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     data   graph     files  public Experimental results: Remineralization experiments to assess the ability of natural
assemblages of bacteria to utilize DOM produced under Si and N limitation (SBDOM project, SBC
LTER project)
   ?     I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_518508

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String S. marinoi experimental design:
DOM was harvested from cultures of S. marinoi 48 hours after the indicated
nutrient became deplete. Harvested media was 2x filtered through 0.2 um
filters. Ambient seawater was the aged seawater media in which the
phytoplankton were grown. Media (6L) and inoculum (2L, 1.2-um filtered water
from the Santa Barbara Channel) were combined in a spigoted polycarbonate
carboy and incubated in the dark at 14C for 4 months.

C. socialis experimental design:
DOM was harvested from cultures of C. socialis 48 hours after the indicated
nutrient became deplete. Harvested media was 2x filtered through 0.2 um
filters. Ambient seawater was the aged seawater media in which the
phytoplankton were grown. Media (6L) and inoculum (2L, 1.2-um filtered water
from the Santa Barbara Channel) were combined in a spigoted polycarbonate
carboy and incubated in the dark at 14C for 4 months.

T. weissflogii experimental design:
DOM was harvested from cultures of T. weissflogii 48 hours after the
indicated nutrient became deplete. Harvested media was 2x filtered through 0.2
um filters. Ambient seawater was the aged seawater media in which the
phytoplankton were grown. Media (6L) and inoculum (2L, 1.2-um filtered water
from the Santa Barbara Channel) were combined in a spigoted polycarbonate
carboy and incubated in the dark at 14C for 4 months.

O. aurita experimental design:
DOM was harvested from cultures of O. aurita 48 hours after the indicated
nutrient became deplete. Harvested media was 2x filtered through 0.2 um
filters. Ambient seawater was the aged seawater media in which the
phytoplankton were grown. This experiment included full-volume inorganic
nutrient controls (ambient seawater with added nitrate and phosphate or
silicate and phosphate, at concentrations comparable to the Si-limited and
N-limited treatments, respectively) and small-volume (2L, same media:inoculum
ratio) controls to look at carry-over from the phytoplankton media that were
sampled less frequently and with fewer replicates (ambient seawater plus f/2
and ambient seawater plus filtered media from an O. aurita culture in
exponential growth, both at concentrations comparable to those at the start of
the O. aurita batch culture grow-ups). Media (6L) and inoculum (2L, 1.2-um
filtered water from the Santa Barbara Channel) were combined in a spigoted
polycarbonate carboy and incubated in the dark at 14C for 4 months.
attribute NC_GLOBAL awards_0_award_nid String 54995
attribute NC_GLOBAL awards_0_award_number String OCE-0850857
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0850857 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Remineralization Experiments
Project: SBDOM
PI: Craig Carlson (UC Santa Barbara, MSI)
Co-PI: Mark Brzezinksi (UC Santa Barbara, MSI)
Version: 09 July 2014
Notes: BDL = Below Detection Limit (0.20 for NO3_NO2; 0.10 for NH4 and PO4)
Lack of standard deviation value indicates replicate was discarded for obvious
contamination and is not reflected in mean value.
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2014-07-09T18:53:14Z
attribute NC_GLOBAL date_modified String 2019-08-28T18:12:35Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.518508.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/518508 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL keywords String abund, ammonium, bact, bact_abund_DAPI, bact_abund_DAPI_sd, bact_abund_FCM, bact_growth_eff, bact_prod, bact_prod_sd, bco, bco-dmo, biological, chemical, chemistry, commerce, concentration, dapi, data, dataset, days, days_elapsed, department, dmo, dna, DNA_sampled, doc, DOC_sd, dSi, earth, Earth Science > Oceans > Ocean Chemistry > Phosphate, eff, elapsed, erddap, fcm, growth, management, mass, mass_concentration_of_phosphate_in_sea_water, nh4, nitrate, nitrite, no2, no3, NO3_NO2, ocean, oceanography, oceans, office, phosphate, po4, point, preliminary, prod, rate, replicate, sampled, science, sea, seawater, species, specifc, specifc_growth_rate, tdn, TDN_sd, time, time_point, treatment, water
attribute NC_GLOBAL keywords_vocabulary String GCMD Science Keywords
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/518508/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/518508 (external link)
attribute NC_GLOBAL param_mapping String {'518508': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/518508/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of California-Santa Barbara
attribute NC_GLOBAL people_0_affiliation_acronym String UCSB-MSI
attribute NC_GLOBAL people_0_person_name String Craig Carlson
attribute NC_GLOBAL people_0_person_nid String 50575
attribute NC_GLOBAL people_0_role String Lead Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of California-Santa Barbara
attribute NC_GLOBAL people_1_affiliation_acronym String UCSB-MSI
attribute NC_GLOBAL people_1_person_name String Mark A. Brzezinski
attribute NC_GLOBAL people_1_person_nid String 50663
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Shannon Rauch
attribute NC_GLOBAL people_2_person_nid String 51498
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String SBDOM,SBC LTER
attribute NC_GLOBAL projects_0_acronym String SBDOM
attribute NC_GLOBAL projects_0_description String This project is also affiliated with the Plumes and Blooms project.
Data:
The following data files have been submitted to BCO-DMO but are not yet available online. Data are restricted until June 2016. Please contact the PI for access prior to public availability:
-- SBDOM10 and SBDOM11 CTD and Niskin bottle data.
The following are available online (see 'Datasets' heading below):
-- SBDOM10 and SBDOM11 cruise plans (available online on deployment pages: PS1009, PS1103)
-- SBDOM10 and SBDOM11 event logs (available online; see 'Datasets' below)
-- Laboratory-based Bloom in a Bottle (BIB) Experiment
-- Laboratory-based Remineralization Experiments
-- SBDOM10 and SBDOM11 data summaries (including CTD data, nutrients, and bacterial production)

Project Description from NSF Award Proposal and Abstract:
Diatom blooms are known to produce prodigious quantities of DOM upon entering nutrient stress with a chemical composition that varies with the type of nutrient limitation (Si or N). This variable composition likely influences the nutritional value of DOM to microbes driving species successions towards functional groups of heterotrophic prokaryotes that are best able to metabolize particular forms of DOM. To date each side of this coupled system of production/consumption has been examined independently. A few studies have examined how limitation by different limiting nutrients affects the chemical character of the DOM produced by phytoplankton, while others have focused on the fate of DOM without detailed understanding of the mechanisms influencing its initial chemical composition.
We propose to investigate the mechanisms determining the character and fate of DOM produced during temperate diatom blooms. Specifically we will investigate how physiological stress on diatoms induced by different limiting nutrients influences the production, chemical composition of DOM and the microbial community structure that respond to it to better understand the mechanisms driving the accumulation and persistence of DOM in marine systems. The research will involve both laboratory and field experiments. The novel aspects of this work are:
1) We will investigate how limitation by either N or Si impacts the quantity and chemical composition of the DOM released by diatoms.
2) Assess how the differences in the chemical composition of the DOM produced under N or Si limitation affect its lability by examining the productivity, growth efficiency and community structure of heterotrophic bacterioplankton responding to the release of substrates.
3) Predicted DOM dynamics based on (1) and (2) will be tested in the field during diatom blooms in the Santa Barbara Channel, California.
While experiments investigating aspects of either 1 or 2 have been conducted successfully in the past (Lancelot, 1983; Billen and Fontigny, 1987; Goldman et al., 1992; Carlson et al.,1999; Cherrier and Bauer, 2004; Conan et al., 2007) ours will be the first study to combine these approaches in an integrated assessment of the mechanisms governing both the production and fate of DOM produced by diatom blooms experiencing limitation by different nutrients.
References:
Lancelot, C. (1983). Factors affecting phytoplankton extracellular release in the Southern Bight of the North Sea. Marine Ecology Progress Series 12: 115-121.
Billen, G. and A. Fontigny (1987). Dynamics of a Phaeocystis -dominated spring bloom in Belgian coastal waters. II. Bacterioplankton dynamics. Mar. Ecol. Prog. Ser. 37: 249-257.
Goldman, J.C., D.A. Hansell and M.R. Dennett (1992). Chemical characterization of three large oceanic diatoms: potential impact on water column chemistry. Marine Ecology Progress Series 88: 257-270.
Carlson, C.A., N.R. Bates, H.W. Ducklow and D.A. Hansell (1999). Estimation of bacterial respiration and growth efficiency in the Ross Sea, Antarctica. Aquatic Microbial Ecology 19: 229-244.
Cherrier, J. and J.E. Bauer (2004). Bacterial utilization of transient plankton-derived dissolved organic carbon and nitrogen inputs in surface ocean waters. Aquatic Microbial Ecology 35(3): 229-241.
Conan, P., M. Sondegaard, T. Kragh, F. Thingstad, M. Pujo-Pay, P.J.l.B. Williams, S. Markager, G. Cauwet, N.H. Borch, D. Evans and B. Rieman (2007). Partitioning of organic production in marine plankton communities: The effects of inorganic nutrient ratios and community composition on new dissolved organic matter. Limnology and Oceanography 52(2): 753-765.
attribute NC_GLOBAL projects_0_end_date String 2014-03
attribute NC_GLOBAL projects_0_geolocation String Pacific California, Santa Barbara Channel
attribute NC_GLOBAL projects_0_name String Mechanisms controlling the production and fate of DOM during diatom blooms
attribute NC_GLOBAL projects_0_project_nid String 2226
attribute NC_GLOBAL projects_0_start_date String 2009-04
attribute NC_GLOBAL projects_1_acronym String SBC LTER
attribute NC_GLOBAL projects_1_description String From http://www.lternet.edu/sites/sbc
The Santa Barbara Coastal LTER is located in the coastal zone of southern California near Santa Barbara. It is bounded by the steep east-west trending Santa Ynez Mountains and coastal plain to the north and the unique Northern Channel Islands archipelago to the south. Santa Barbara Coastal Long-Term Ecological Research (SBC) Project is headquartered at the University of California, Santa Barbara, and is part of the National Science Foundation’s (NSF) Long-Term Ecological Research (LTER) Network.
The research focus of SBC LTER is on ecological systems at the land-ocean margin. Although there is increasing concern about the impacts of human activities on coastal watersheds and nearshore marine environments, there have been few long-term studies of the linkages among oceanic, reef, sandy beaches, wetland, and upland habitats. SBC LTER is helping to fill this gap by studying the effects of oceanic and coastal watershed influences on kelp forests in the Santa Barbara Channel located off the coast of southern California. The primary research objective of SBC LTER is to investigate the relative importance of land vs. ocean processes in structuring giant kelp (Macrocystis pyrifera) forest ecosystems for different conditions of land use, climate and ocean influences.
SBC LTER Data: The Santa Barbara Coastal (SBC) LTER data are managed by and available directly from the SBC project data site URL shown above.  If there are any datasets listed below, they are data sets that were collected at or near the SBC LTER sampling locations, and funded by NSF OCE as ancillary projects related to the SBC LTER core research themes. See the SBC LTER Data Overview page for access to data and information about data management policies.
attribute NC_GLOBAL projects_1_geolocation String Southern California Coastal Zone
attribute NC_GLOBAL projects_1_name String Santa Barbara Coastal Long Term Ecological Research site
attribute NC_GLOBAL projects_1_project_nid String 2227
attribute NC_GLOBAL projects_1_project_website String http://sbc.lternet.edu/ (external link)
attribute NC_GLOBAL projects_1_start_date String 2000-04
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Culture studies of diatoms that dominate spring blooms in the Santa Barbara Channel were used to examine the effects of N and Si stress on the magnitude of production and the chemical composition of DOM (see the BIB Experiments dataset). The ability of natural assemblages of bacteria to utilize DOM produced under Si and N limitation was then assessed using remineralization experiments. Species examined in the remineralization experiments include: Skeletonema marinoi, Chaetoceros socialis, Thalassiosira weissflogii, and Odontella aurita.
attribute NC_GLOBAL title String Experimental results: Remineralization experiments to assess the ability of natural assemblages of bacteria to utilize DOM produced under Si and N limitation (SBDOM project, SBC LTER project)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable species   String  
attribute species bcodmo_name String species
attribute species description String Name of the species.
attribute species long_name String Species
attribute species units String text
variable treatment   String  
attribute treatment bcodmo_name String treatment
attribute treatment description String Treatment condition.
attribute treatment long_name String Treatment
attribute treatment units String text
variable replicate   byte  
attribute replicate _FillValue byte 127
attribute replicate actual_range byte 1, 2
attribute replicate bcodmo_name String replicate
attribute replicate description String Replicate number.
attribute replicate long_name String Replicate
attribute replicate units String integer
variable time_point   byte  
attribute time_point _FillValue byte 127
attribute time_point actual_range byte 0, 20
attribute time_point bcodmo_name String time_point
attribute time_point description String Sampling time point.
attribute time_point long_name String Time Point
attribute time_point units String integer
variable days_elapsed   float  
attribute days_elapsed _FillValue float NaN
attribute days_elapsed actual_range float 0.0, 112.0
attribute days_elapsed bcodmo_name String time_elapsed
attribute days_elapsed description String Number of days elapsed since the start of the experiment.
attribute days_elapsed long_name String Days Elapsed
attribute days_elapsed nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/ (external link)
attribute days_elapsed units String number
variable DOC   float  
attribute DOC _FillValue float NaN
attribute DOC actual_range float 59.2, 110.4
attribute DOC bcodmo_name String DOC
attribute DOC description String Dissolved organic carbon.
attribute DOC long_name String DOC
attribute DOC nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CORGZZZX/ (external link)
attribute DOC units String micromoles per Liter (umol/L)
variable DOC_sd   float  
attribute DOC_sd _FillValue float NaN
attribute DOC_sd actual_range float 0.0, 6.1
attribute DOC_sd bcodmo_name String standard deviation
attribute DOC_sd colorBarMaximum double 50.0
attribute DOC_sd colorBarMinimum double 0.0
attribute DOC_sd description String Standard deviation of DOC.
attribute DOC_sd long_name String DOC Sd
attribute DOC_sd units String micromoles per Liter (umol/L)
variable TDN   float  
attribute TDN _FillValue float NaN
attribute TDN actual_range float 5.0, 23.9
attribute TDN bcodmo_name String Total Dissolved Nitrogren
attribute TDN description String Total dissolved nitrogen.
attribute TDN long_name String TDN
attribute TDN units String micromoles per Liter (umol/L)
variable TDN_sd   float  
attribute TDN_sd _FillValue float NaN
attribute TDN_sd actual_range float 0.0, 1.4
attribute TDN_sd bcodmo_name String standard deviation
attribute TDN_sd colorBarMaximum double 50.0
attribute TDN_sd colorBarMinimum double 0.0
attribute TDN_sd description String Standard deviation of TDN.
attribute TDN_sd long_name String TDN SD
attribute TDN_sd units String micromoles per Liter (umol/L)
variable NO3_NO2   String  
attribute NO3_NO2 bcodmo_name String NO3_NO2
attribute NO3_NO2 description String Nitrate and nitrite.
attribute NO3_NO2 long_name String NO3 NO2
attribute NO3_NO2 units String micromoles per Liter (umol/L)
variable NH4   String  
attribute NH4 bcodmo_name String Ammonium
attribute NH4 description String Concentration of NH4.
attribute NH4 long_name String NH4
attribute NH4 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/AMONAAZX/ (external link)
attribute NH4 units String micromoles per Liter (umol/L)
variable PO4   float  
attribute PO4 _FillValue float NaN
attribute PO4 actual_range float 0.16, 6.03
attribute PO4 bcodmo_name String PO4
attribute PO4 description String Concentration of PO4.
attribute PO4 long_name String Mass Concentration Of Phosphate In Sea Water
attribute PO4 units String micromoles per Liter (umol/L)
variable dSi   float  
attribute dSi _FillValue float NaN
attribute dSi actual_range float 0.07, 12.78
attribute dSi bcodmo_name String unknown
attribute dSi description String Dissolved SO4.
attribute dSi long_name String D Si
attribute dSi units String micromoles per Liter (umol/L)
variable bact_abund_FCM   float  
attribute bact_abund_FCM _FillValue float NaN
attribute bact_abund_FCM actual_range float 9.01E7, 5.6700001E9
attribute bact_abund_FCM bcodmo_name String unknown
attribute bact_abund_FCM description String Bacterial cell abundance measured via flow cytometer using SYBR Green stain.
attribute bact_abund_FCM long_name String Bact Abund FCM
attribute bact_abund_FCM units String cells per Liter (cells/L)
variable bact_abund_DAPI   float  
attribute bact_abund_DAPI _FillValue float NaN
attribute bact_abund_DAPI actual_range float 5.1E8, 1.8E9
attribute bact_abund_DAPI bcodmo_name String unknown
attribute bact_abund_DAPI description String Bacterial cell abundance measured via epifluorescence microscopy using DAPI stain.
attribute bact_abund_DAPI long_name String Bact Abund DAPI
attribute bact_abund_DAPI units String cells per Liter (cells/L)
variable bact_abund_DAPI_sd   float  
attribute bact_abund_DAPI_sd _FillValue float NaN
attribute bact_abund_DAPI_sd actual_range float 6.7E7, 4.7E8
attribute bact_abund_DAPI_sd bcodmo_name String standard deviation
attribute bact_abund_DAPI_sd colorBarMaximum double 50.0
attribute bact_abund_DAPI_sd colorBarMinimum double 0.0
attribute bact_abund_DAPI_sd description String Standard deviation of bact_abund_DAPI.
attribute bact_abund_DAPI_sd long_name String Bact Abund DAPI Sd
attribute bact_abund_DAPI_sd units String cells per Liter (cells/L)
variable bact_prod   float  
attribute bact_prod _FillValue float NaN
attribute bact_prod actual_range float 7.8, 2136.64
attribute bact_prod bcodmo_name String unknown
attribute bact_prod description String Bacterial production measured via 3H leucine uptake.
attribute bact_prod long_name String Bact Prod
attribute bact_prod units String picomoles Leucine per Liter per hour (pmol Leu/L/hr)
variable bact_prod_sd   float  
attribute bact_prod_sd _FillValue float NaN
attribute bact_prod_sd actual_range float 0.0, 197.78
attribute bact_prod_sd bcodmo_name String standard deviation
attribute bact_prod_sd colorBarMaximum double 50.0
attribute bact_prod_sd colorBarMinimum double 0.0
attribute bact_prod_sd description String Standard deviation of bact_prod.
attribute bact_prod_sd long_name String Bact Prod Sd
attribute bact_prod_sd units String picomoles Leucine per Liter per hour (pmol Leu/L/hr)
variable specifc_growth_rate   float  
attribute specifc_growth_rate _FillValue float NaN
attribute specifc_growth_rate actual_range float 0.1, 1.9
attribute specifc_growth_rate bcodmo_name String unknown
attribute specifc_growth_rate description String Specific growth rate (µ) over exponential growth phase.
attribute specifc_growth_rate long_name String Specifc Growth Rate
attribute specifc_growth_rate units String per day
variable bact_growth_eff   byte  
attribute bact_growth_eff _FillValue byte 127
attribute bact_growth_eff actual_range byte 8, 48
attribute bact_growth_eff bcodmo_name String unknown
attribute bact_growth_eff description String Bacterial growth efficiency over exponential growth phase.
attribute bact_growth_eff long_name String Bact Growth Eff
attribute bact_growth_eff units String unitless
variable DNA_sampled   String  
attribute DNA_sampled bcodmo_name String unknown
attribute DNA_sampled description String yes = sample was collected; analyzed samples still in QC.
attribute DNA_sampled long_name String DNA Sampled
attribute DNA_sampled units String yes or no

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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