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     data   graph     files  public Chemical composition of diffuse flow vent fluids collected from the Crab Spa site at East
Pacific Rise during the AT26-10 oceanographic expedition, Jan. 2014 (Microbial Communities at
Deep-Sea Vents project)
   ?     I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_529026

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String These samples were collected with Isobaric-Gas-Tight samplers and processed
onboard R/V Atlantis. The IGT samplers were deployed by Dr. Jeff Seewald from
WHOI. Chemical analysis of the major cation/anion species was conducted at the
Geophysical Lab, Carnegie Institution of Washington\u00a0
attribute NC_GLOBAL awards_0_award_nid String 54989
attribute NC_GLOBAL awards_0_award_number String OCE-1136608
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1136608 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Crab Spa chemistry
East Pacific Rise vent

D. Foustoukos (Carnegie Inst. of Washington)

version: 1 Oct. 2014
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2014-09-30T19:36:39Z
attribute NC_GLOBAL date_modified String 2017-02-13T19:30:24Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.682102
attribute NC_GLOBAL Easternmost_Easting double -104.2915
attribute NC_GLOBAL geospatial_lat_max double 9.83992
attribute NC_GLOBAL geospatial_lat_min double 9.83987
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -104.2915
attribute NC_GLOBAL geospatial_lon_min double -104.29162
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 2512.0
attribute NC_GLOBAL geospatial_vertical_min double 2504.0
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/529026 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Ion Chromatograph
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Metrohm/Dionex, for cation and anion species.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 529035
attribute NC_GLOBAL instruments_0_description String Ion chromatography is a form of liquid chromatography that measures concentrations of ionic species by separating them based on their interaction with a resin. Ionic species separate differently depending on species type and size. Ion chromatographs are able to measure concentrations of major anions, such as fluoride, chloride, nitrate, nitrite, and sulfate, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium in the parts-per-billion (ppb) range. (from http://serc.carleton.edu/microbelife/research_methods/biogeochemical/ic.html)
attribute NC_GLOBAL instruments_0_instrument_name String Ion Chromatograph
attribute NC_GLOBAL instruments_0_instrument_nid String 662
attribute NC_GLOBAL instruments_0_supplied_name String Ion Chromatograph
attribute NC_GLOBAL instruments_1_acronym String IGT Sampler
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 529050
attribute NC_GLOBAL instruments_1_description String Isobaric Gas Tight (IGT) samplers, designed and built by scientists and engineers at WHOI, are titanium instruments designed to be used with deep submergence vehicles to sample corrosive hydrothermal vent fluids at high temperature and high pressure. The IGT prevents the sampled fluid from degassing as pressure decreases during the vehicle’s ascent to the surface.
attribute NC_GLOBAL instruments_1_instrument_name String Isobaric Gas-Tight Sampler
attribute NC_GLOBAL instruments_1_instrument_nid String 529049
attribute NC_GLOBAL instruments_1_supplied_name String IGT Sampler
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, chemical, Cl_stdev, data, dataset, date, depth, deviation, dmo, erddap, latitude, longitude, management, oceanography, office, preliminary, sample, site, so4, SO4_stdev, standard, standard deviation, stdev, time
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/529026/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/529026 (external link)
attribute NC_GLOBAL Northernmost_Northing double 9.83992
attribute NC_GLOBAL param_mapping String {'529026': {'lat': 'master - latitude', 'depth': 'master - depth', 'lon': 'master - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/529026/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Carnegie Institution for Science
attribute NC_GLOBAL people_0_affiliation_acronym String CIS
attribute NC_GLOBAL people_0_person_name String Dionysis Foustoukos
attribute NC_GLOBAL people_0_person_nid String 51518
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Nancy Copley
attribute NC_GLOBAL people_1_person_nid String 50396
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String Microbial Communities at Deep-Sea Vents
attribute NC_GLOBAL projects_0_acronym String Microbial Communities at Deep-Sea Vents
attribute NC_GLOBAL projects_0_description String Deep-sea hydrothermal vents, first discovered in 1977, are poster child ecosystems where microbial chemosynthesis rather than photosynthesis is the primary source of organic carbon. Significant gaps remain in our understanding of the underlying microbiology and biogeochemistry of these fascinating ecosystems. Missing are the identification of specific microorganisms mediating critical reactions in various geothermal systems, metabolic pathways used by the microbes, rates of the catalyzed reactions, amounts of organic carbon being produced, and the larger role of these ecosystems in global biogeochemical cycles. To fill these gaps, the investigators will conduct a 3-year interdisciplinary, international hypothesis-driven research program to understand microbial processes and their quantitative importance at deep-sea vents. Specifically, the investigators will address the following objectives: 1. Determine key relationships between the taxonomic, genetic and functional diversity, as well as the mechanisms of energy and carbon transfer, in deep-sea hydrothermal vent microbial communities. 2. Identify the predominant metabolic pathways and thus the main energy sources driving chemoautotrophic production in high and low temperature diffuse flow vents. 3. Determine energy conservation efficiency and rates of aerobic and anaerobic chemosynthetic primary productivity in high and low temperature diffuse flow vents. 4. Determine gene expression patterns in diffuse-flow vent microbial communities during attachment to substrates and the development of biofilms.


Integration: To address these objectives and to characterize the complexity of microbially-catalyzed processes at deep-sea vents at a qualitatively new level, we will pursue an integrated approach that couples an assessment of taxonomic diversity using cultivation-dependent and -independent approaches with methodologies that address genetic diversity, including a) metagenomics (genetic potential and diversity of community), b) single cell genomics (genetic potential and diversity of uncultured single cells), c) meta-transcriptomics and -proteomics (identification and function of active community members, realized potential of the community). To assess function and response to the environment, these approaches will be combined with 1) measurement of in situ rates of chemoautotrophic production, 2) geochemical characterization of microbial habitats, and 3) shipboard incubations under simulated in situ conditions (hypothesis testing under controlled physicochemical conditions). Network approaches and mathematical simulation will be used to reconstruct the metabolic network of the natural communities. A 3-day long project meeting towards the end of the second year will take place in Woods Hole. This Data Integration and Synthesis meeting will allow for progress reports and presentations from each PI, postdoc, and/or student, with the aim of synthesizing data generated to facilitate the preparation of manuscripts.


Intellectual Merit. Combining the community expression profile with diversity and metagenomic analyses as well as process and habitat characterization will be unique to hydrothermal vent microbiology. The approach will provide new insights into the functioning of deep-sea vent microbial communities and the constraints regulating the interactions between the microbes and their abiotic and biotic environment, ultimately enabling us to put these systems into a quantitative framework and thus a larger global context.


Broader Impacts. This is an interdisciplinary and collaborative effort between 4 US and 4 foreign institutions, creating unique opportunities for networking and fostering international collaborations. This will also benefit the involved students (2 graduate, several undergraduate) and 2 postdoctoral associates. This project will directly contribute to many educational and public outreach activities of the involved PIs, including the WHOI Dive & Discover program; single cell genomics workshops and Cafe Scientifique (Bigelow); REU (WHOI, Bigelow, CIW); COSEE and RIOS (Rutgers), and others. The proposed research fits with the focus of a number of multidisciplinary and international initiatives, in which PIs are active members (SCOR working group on Hydrothermal energy and the ocean carbon cycle, http://www.scorint. org/Working_Groups/wg135.htm; Deep Carbon Observatory at CIW, https://dco.gl.ciw.edu/; Global Biogeochemical Flux (GBF) component of the Ocean Observatories Initiative (OOI), https://www.whoi.edu/GBF-OOI/page.do?pid=41475)
attribute NC_GLOBAL projects_0_end_date String 2014-09
attribute NC_GLOBAL projects_0_name String An Integrated Study of Energy Metabolism, Carbon Fixation, and Colonization Mechanisms in Chemosynthetic Microbial Communities at Deep-Sea Vents
attribute NC_GLOBAL projects_0_project_nid String 2216
attribute NC_GLOBAL projects_0_start_date String 2011-10
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 9.83987
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String This dataset includes\u00a0chemical composition (Cl, SO4, Na, K, Mg, and Ca
concentrations) of diffuse flow vent fluids collected from the Crab Spa
(9.8398\u00ba N, 104.2913\u00ba W) site at East Pacific Rise during the
RV/Atlantic AT26-10 oceanographic expedition, Jan. 2014.
attribute NC_GLOBAL title String Chemical composition of diffuse flow vent fluids collected from the Crab Spa site at East Pacific Rise during the AT26-10 oceanographic expedition, Jan. 2014 (Microbial Communities at Deep-Sea Vents project)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -104.29162
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable date   String  
attribute date bcodmo_name String date
attribute date description String sampling date. Format: YYYY-MM-DD
attribute date long_name String Date
attribute date nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute date source_name String date
attribute date time_precision String 1970-01-01
attribute date units String unitless
variable site   String  
attribute site bcodmo_name String site
attribute site description String sample location
attribute site long_name String Site
attribute site units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 9.83987, 9.83992
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String latitude; north is positive
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -104.29162, -104.2915
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String longitude; east is positive
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable depth   double  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth _FillValue double NaN
attribute depth actual_range double 2504.0, 2512.0
attribute depth axis String Z
attribute depth bcodmo_name String depth
attribute depth colorBarMaximum double 8000.0
attribute depth colorBarMinimum double -8000.0
attribute depth colorBarPalette String TopographyDepth
attribute depth description String depth where sample was taken
attribute depth ioos_category String Location
attribute depth long_name String Depth
attribute depth nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable sample   String  
attribute sample bcodmo_name String sample
attribute sample description String sample identification
attribute sample long_name String Sample
attribute sample nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute sample units String unitless
variable Cl   short  
attribute Cl _FillValue short 32767
attribute Cl actual_range short 533, 632
attribute Cl bcodmo_name String unknown
attribute Cl description String chloride ion concentration
attribute Cl long_name String CL
attribute Cl units String mmoles/kg
variable Cl_stdev   byte  
attribute Cl_stdev _FillValue byte 127
attribute Cl_stdev actual_range byte 9, 21
attribute Cl_stdev bcodmo_name String standard deviation
attribute Cl_stdev description String chloride ion concentration standard deviation
attribute Cl_stdev long_name String Cl Stdev
attribute Cl_stdev units String mmoles/kg
variable SO4   float  
attribute SO4 _FillValue float NaN
attribute SO4 actual_range float 25.2, 30.3
attribute SO4 bcodmo_name String SO4
attribute SO4 description String sulfate concentration
attribute SO4 long_name String SO4
attribute SO4 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/SPHTMAXX/ (external link)
attribute SO4 units String mmoles/kg
variable SO4_stdev   float  
attribute SO4_stdev _FillValue float NaN
attribute SO4_stdev actual_range float 0.2, 5.0
attribute SO4_stdev bcodmo_name String standard deviation
attribute SO4_stdev description String sulfate concentration standard deviation
attribute SO4_stdev long_name String SO4 Stdev
attribute SO4_stdev units String mmoles/kg
variable Na   String  
attribute Na bcodmo_name String Na
attribute Na description String sodium ion concentration
attribute Na units String mmoles/kg
variable K   float  
attribute K _FillValue float NaN
attribute K actual_range float 9.7, 12.2
attribute K bcodmo_name String K
attribute K description String potassium ion concentration
attribute K long_name String K
attribute K units String mmoles/kg
variable Mg   float  
attribute Mg _FillValue float NaN
attribute Mg actual_range float 42.2, 55.6
attribute Mg bcodmo_name String Mg
attribute Mg description String magnesium ion concentration
attribute Mg long_name String MG
attribute Mg units String mmoles/kg
variable Ca   float  
attribute Ca _FillValue float NaN
attribute Ca actual_range float 19.7, 22.1
attribute Ca bcodmo_name String Ca
attribute Ca description String calcium ion concentration
attribute Ca long_name String Ca
attribute Ca units String mmoles/kg

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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