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Title Sum-
Institution Dataset ID
     data   graph     files  public Host-symbiont respiration related to symbiont density; anemones from Key Largo
from (AnemoneOA project)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Full details of the experimental design are provided in Hawkins et al., 2016
attribute NC_GLOBAL awards_0_award_nid String 628966
attribute NC_GLOBAL awards_0_award_number String EF-1316055
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1316055 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Emerging Frontiers Division
attribute NC_GLOBAL awards_0_funding_acronym String NSF EF
attribute NC_GLOBAL awards_0_funding_source_nid String 392
attribute NC_GLOBAL awards_0_program_manager String Irwin Forseth
attribute NC_GLOBAL awards_0_program_manager_nid String 520504
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Anemone host and symbiont respiration
per symbiont density in laboratory setting
Fresh anemones from Key Largo
M. Warner, PI
CS=Citrate Synthase
Version 14 June 2016
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2016-06-17T14:55:35Z
attribute NC_GLOBAL date_modified String 2019-07-02T15:44:44Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.649708.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/649708 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to visualize cells' chlorophyll a fluorescence.  [EVOS system, ThermoFisher (Life Technologies), Waltham, MA, USA; excitation: 628 ± 20 nm, emission: 692 ± 20 nm].
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 708840
attribute NC_GLOBAL instruments_0_description String Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB06/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Microscope-Fluorescence
attribute NC_GLOBAL instruments_0_instrument_nid String 695
attribute NC_GLOBAL instruments_0_supplied_name String fluorescence microscope
attribute NC_GLOBAL instruments_1_acronym String Hemocytometer
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used along with fluorescence microscope to quantify microbial densities.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 708835
attribute NC_GLOBAL instruments_1_description String A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from:
attribute NC_GLOBAL instruments_1_instrument_name String Hemocytometer
attribute NC_GLOBAL instruments_1_instrument_nid String 704
attribute NC_GLOBAL instruments_1_supplied_name String Improved Neubauer hemocytometer
attribute NC_GLOBAL instruments_2_acronym String Spectrophotometer
attribute NC_GLOBAL instruments_2_dataset_instrument_description String ThermoFisher, Waltham, MA, USA
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 708844
attribute NC_GLOBAL instruments_2_description String An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB20/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Spectrophotometer
attribute NC_GLOBAL instruments_2_instrument_nid String 707
attribute NC_GLOBAL instruments_2_supplied_name String NanoDrop spectrophotometer
attribute NC_GLOBAL instruments_3_dataset_instrument_description String an oxygen sensitive optode (Fibox 4, PreSens Gmbh, Regensburg, Germany).
attribute NC_GLOBAL instruments_3_dataset_instrument_nid String 708834
attribute NC_GLOBAL instruments_3_description String An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.
attribute NC_GLOBAL instruments_3_instrument_name String Optode
attribute NC_GLOBAL instruments_3_instrument_nid String 727
attribute NC_GLOBAL instruments_3_supplied_name String Oxygen sensitive optode
attribute NC_GLOBAL instruments_4_acronym String Thermal Cycler
attribute NC_GLOBAL instruments_4_dataset_instrument_description String AB-7500 real-time QPCR system [ThermoFisher (Applied Biosystems), Waltham, MA, USA], with the following cycling conditions: 94◦ C for 10 min, followed by 40 cycles of 94◦C for 15 s, 60◦C for 1 min, and 72◦C for 15 s.
attribute NC_GLOBAL instruments_4_dataset_instrument_nid String 708845
attribute NC_GLOBAL instruments_4_description String General term for a laboratory apparatus commonly used for performing polymerase chain reaction (PCR). The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps.

(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)
attribute NC_GLOBAL instruments_4_instrument_name String PCR Thermal Cycler
attribute NC_GLOBAL instruments_4_instrument_nid String 471582
attribute NC_GLOBAL instruments_4_supplied_name String ThermoFisher QPCR system
attribute NC_GLOBAL instruments_5_dataset_instrument_description String Measured baseline absorbance at 412nm. (Fluostar Omega, BMG, Cary, NC, USA)
attribute NC_GLOBAL instruments_5_dataset_instrument_nid String 743373
attribute NC_GLOBAL instruments_5_description String Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: https://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.
attribute NC_GLOBAL instruments_5_instrument_name String plate reader
attribute NC_GLOBAL instruments_5_instrument_nid String 528693
attribute NC_GLOBAL instruments_5_supplied_name String microplate reader
attribute NC_GLOBAL keywords String activity, anemone, at96, bco, bco-dmo, biological, biomass, chemical, co1, data, dataset, dens, dmo, dna, erddap, fraction, genome, genome_ratio_AT96, genome_ratio_CO1, genome_ratio_CO1_Pro, holobiont, holobiont_resp_total, host, host_CS_activity, host_CS_activity_product_total, host_DNA_total, host_symb_dens_normal, management, normal, oceanography, office, preliminary, pro, product, prot, prot_normal_resp, protein, ratio, resp, respir, sample, symb, symb_biomass_fraction, symb_CS_activity, symb_respir_fraction, total, total_anemone_CS_activity, total_host_protein
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/649708/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/649708 (external link)
attribute NC_GLOBAL param_mapping String {'649708': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/649708/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Delaware
attribute NC_GLOBAL people_0_person_name String Mark E. Warner
attribute NC_GLOBAL people_0_person_nid String 51387
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Ms Dicky Allison
attribute NC_GLOBAL people_1_person_nid String 50382
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String AnemoneOA
attribute NC_GLOBAL projects_0_acronym String AnemoneOA
attribute NC_GLOBAL projects_0_description String The projected rise in carbon dioxide (CO2) in the atmosphere is considered a primary threat to marine systems throughout the world due to both ocean acidification and rising ocean temperatures. Coral reefs are very sensitive to these projected changes in the earth's climate, with continued losses in growth as well as disruption (also known as bleaching) in the symbiotic relationship between the algae (Symbiodinium) living within a diversity of host animals, including stony corals, soft corals and sea anemones. While much information has been gleaned as to how acidification may affect stony corals, considerably less is known about the interactive effects of acidification and temperature to other symbiotic anthozoans.
To this end, this proposal will investigate the long-term impacts of elevated CO2 and temperature on the model sea anemone, Aiptasia pallida, while harboring four different genotypes of Symbiodinium. The primary goals of this project are (1) to determine the sensitivity and capacity for acclimation in molecular and physiological processes while exposed to elevated CO2 and temperature, and (2) to assess the degree to which acclimated adult animals may confer (or transfer) an imprinted physiological characteristic to the next generation of asexual offspring. A series of long-term experiments will be conducted with each animal/algal combination (holobiont) in order to collect initial (3 month) stress markers and genomic data and then follow animal response and asexual reproduction through several generations for one year. The possibility for enhanced resilience or acclimation will be measured by tracking the recovery of each holobiont, followed by repeated exposure to elevated temperature while held in high CO2. This project will tease apart fine scale mechanisms of stress, acclimation, or amelioration that may vary as a function of algal genotype and host animal response, and the degree to which environmental imprinting may pre-acclimate propagules. Project results will provide information regarding how future acidification and warming will affect cnidarian-algal symbioses, and the fundamental profile of their flexibility in stress response processes across organismal, metabolic, genomic and epigenetic scales.
attribute NC_GLOBAL projects_0_end_date String 2016-06
attribute NC_GLOBAL projects_0_geolocation String Lewes, Delaware
attribute NC_GLOBAL projects_0_name String Ocean Acidification: Understanding the Impact of CO2 and Temperature on the Physiological, Genetic, and Epigenetic Response of a Model Sea Anemone System with Different Symbionts
attribute NC_GLOBAL projects_0_project_nid String 628967
attribute NC_GLOBAL projects_0_start_date String 2013-07
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Host-symbiont respiration related to symbiont density; anemones from Key Largo from (AnemoneOA project)
attribute NC_GLOBAL title String Host-symbiont respiration related to symbiont density; anemones from Key Largo from (AnemoneOA project)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable sample   String  
attribute sample bcodmo_name String sample
attribute sample description String sample ID number
attribute sample long_name String Sample
attribute sample nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute sample units String unitless
variable holobiont_resp_total   float  
attribute holobiont_resp_total _FillValue float NaN
attribute holobiont_resp_total actual_range float 0.2603, 1.6616
attribute holobiont_resp_total bcodmo_name String respiration
attribute holobiont_resp_total description String total holobiont (anemone)respiration
attribute holobiont_resp_total long_name String Holobiont Resp Total
attribute holobiont_resp_total units String micromoles of oxygen per hour
variable prot_normal_resp   float  
attribute prot_normal_resp _FillValue float NaN
attribute prot_normal_resp actual_range float 0.6121, 1.9819
attribute prot_normal_resp bcodmo_name String respiration
attribute prot_normal_resp description String Host protein-normalised holobiont respiration
attribute prot_normal_resp long_name String Prot Normal Resp
attribute prot_normal_resp units String micromoles of oxygen per hour per milligram protein
variable host_CS_activity_product_total   float  
attribute host_CS_activity_product_total _FillValue float NaN
attribute host_CS_activity_product_total actual_range float 0.014, 0.1088
attribute host_CS_activity_product_total bcodmo_name String metabolic_enzyme
attribute host_CS_activity_product_total description String Total host citrate synthase activity; the product of host citrate synthase activity and total host protein
attribute host_CS_activity_product_total long_name String Host CS Activity Product Total
attribute host_CS_activity_product_total units String Units per milligram protein X milligrams
variable host_CS_activity   float  
attribute host_CS_activity _FillValue float NaN
attribute host_CS_activity actual_range float 0.0343, 0.1794
attribute host_CS_activity bcodmo_name String metabolic_enzyme
attribute host_CS_activity description String Host citrate synthase specific activity
attribute host_CS_activity long_name String Host CS Activity
attribute host_CS_activity units String units per milligram protein
variable total_host_protein   float  
attribute total_host_protein _FillValue float NaN
attribute total_host_protein actual_range float 0.1369, 2.1751
attribute total_host_protein bcodmo_name String unknown
attribute total_host_protein description String Total host protein
attribute total_host_protein long_name String Total Host Protein
attribute total_host_protein units String milligrams
variable symb_CS_activity   float  
attribute symb_CS_activity _FillValue float NaN
attribute symb_CS_activity actual_range float 0.0334, 0.1677
attribute symb_CS_activity bcodmo_name String metabolic_enzyme
attribute symb_CS_activity description String Symbiont citrate synthase specific activity
attribute symb_CS_activity long_name String Symb CS Activity
attribute symb_CS_activity units String Units per milligram protein
variable total_anemone_CS_activity   float  
attribute total_anemone_CS_activity _FillValue float NaN
attribute total_anemone_CS_activity actual_range float 0.0144, 0.1199
attribute total_anemone_CS_activity bcodmo_name String metabolic_enzyme
attribute total_anemone_CS_activity description String Total holobiont citrate synthase activity
attribute total_anemone_CS_activity long_name String Total Anemone CS Activity
attribute total_anemone_CS_activity units String Units
variable host_DNA_total   double  
attribute host_DNA_total _FillValue double NaN
attribute host_DNA_total actual_range double 0.104517579, 18.21235118
attribute host_DNA_total bcodmo_name String unknown
attribute host_DNA_total description String Total host DNA content
attribute host_DNA_total long_name String Host DNA Total
attribute host_DNA_total units String micrograms
variable host_symb_dens_normal   float  
attribute host_symb_dens_normal _FillValue float NaN
attribute host_symb_dens_normal actual_range float 1.0364, 13.3863
attribute host_symb_dens_normal bcodmo_name String abundance
attribute host_symb_dens_normal description String Host protein-normalised symbiont density
attribute host_symb_dens_normal long_name String Host Symb Dens Normal
attribute host_symb_dens_normal nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/B070/ (external link)
attribute host_symb_dens_normal units String million cells per mg protein
variable symb_biomass_fraction   float  
attribute symb_biomass_fraction _FillValue float NaN
attribute symb_biomass_fraction actual_range float 0.0291, 0.2336
attribute symb_biomass_fraction bcodmo_name String unknown
attribute symb_biomass_fraction colorBarMaximum double 1.0
attribute symb_biomass_fraction colorBarMinimum double 0.0
attribute symb_biomass_fraction description String Symbiont biomass fraction (calculated as total symbiont protein [mg]/total host+symbiont protein [mg])
attribute symb_biomass_fraction long_name String Symb Biomass Fraction
attribute symb_biomass_fraction units String dimensionless
variable symb_respir_fraction   float  
attribute symb_respir_fraction _FillValue float NaN
attribute symb_respir_fraction actual_range float 0.00665, 0.1981
attribute symb_respir_fraction bcodmo_name String unknown
attribute symb_respir_fraction colorBarMaximum double 1.0
attribute symb_respir_fraction colorBarMinimum double 0.0
attribute symb_respir_fraction description String Symbiont respiration fraction (calculated as total symbiont citrate synthase activity [U]/total host+symbiont citrate synthase activity [U])
attribute symb_respir_fraction long_name String Symb Respir Fraction
attribute symb_respir_fraction units String dimensionless
variable genome_ratio_CO1   float  
attribute genome_ratio_CO1 _FillValue float NaN
attribute genome_ratio_CO1 actual_range float 20.2126, 406.0552
attribute genome_ratio_CO1 bcodmo_name String unknown
attribute genome_ratio_CO1 description String Mitochondrial:nuclear genome ratio calculated using CO1 (QiaAmp DNA Mini Kit)
attribute genome_ratio_CO1 long_name String Genome Ratio CO1
attribute genome_ratio_CO1 units String dimensionless
variable genome_ratio_AT96   float  
attribute genome_ratio_AT96 _FillValue float NaN
attribute genome_ratio_AT96 actual_range float 13.1141, 395.0889
attribute genome_ratio_AT96 bcodmo_name String unknown
attribute genome_ratio_AT96 description String Mitochondrial:nuclear genome ratio calculated using ATP6 (QiaAmp DNA Mini Kit)
attribute genome_ratio_AT96 long_name String Genome Ratio AT96
attribute genome_ratio_AT96 units String dimensionless
variable genome_ratio_CO1_Pro   float  
attribute genome_ratio_CO1_Pro _FillValue float NaN
attribute genome_ratio_CO1_Pro actual_range float 50.8763, 352.7607
attribute genome_ratio_CO1_Pro bcodmo_name String unknown
attribute genome_ratio_CO1_Pro description String Mitochondrial:nuclear genome ratio calculated using CO1 (Promega Wizard Kit)
attribute genome_ratio_CO1_Pro long_name String Genome Ratio CO1 Pro
attribute genome_ratio_CO1_Pro units String dimensionless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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