ERDDAP > info > bcodmo_dataset_649708

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Host-Symbiont respiration relationship] - Host-symbiont respiration related to symbiont density; anemones from Key Largo from (AnemoneOA project) (Ocean Acidification: Understanding the Impact of CO2 and Temperature on the Physiological, Genetic, and Epigenetic Response of a Model Sea Anemone System with Different Symbionts)		I   M	background			BCO-DMO	bcodmo_dataset_649708

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Full details of the experimental design are provided in Hawkins et al., 2016
attribute	NC_GLOBAL	awards_0_award_nid	String	628966
attribute	NC_GLOBAL	awards_0_award_number	String	EF-1316055
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1316055
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Emerging Frontiers Division
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF EF
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	392
attribute	NC_GLOBAL	awards_0_program_manager	String	Irwin Forseth
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	520504
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Anemone host and symbiont respiration per symbiont density in laboratory setting Fresh anemones from Key Largo M. Warner, PI CS=Citrate Synthase Version 14 June 2016
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2016-06-17T14:55:35Z
attribute	NC_GLOBAL	date_modified	String	2019-07-02T15:44:44Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.649708.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/649708
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used to visualize cells' chlorophyll a fluorescence.  [EVOS system, ThermoFisher (Life Technologies), Waltham, MA, USA; excitation: 628 ± 20 nm, emission: 692 ± 20 nm].
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	708840
attribute	NC_GLOBAL	instruments_0_description	String	Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB06/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Microscope-Fluorescence
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	695
attribute	NC_GLOBAL	instruments_0_supplied_name	String	fluorescence microscope
attribute	NC_GLOBAL	instruments_1_acronym	String	Hemocytometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Used along with fluorescence microscope to quantify microbial densities.
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	708835
attribute	NC_GLOBAL	instruments_1_description	String	A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from: http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Hemocytometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	704
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Improved Neubauer hemocytometer
attribute	NC_GLOBAL	instruments_2_acronym	String	Spectrophotometer
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	ThermoFisher, Waltham, MA, USA
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	708844
attribute	NC_GLOBAL	instruments_2_description	String	An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.
attribute	NC_GLOBAL	instruments_2_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB20/
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Spectrophotometer
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	707
attribute	NC_GLOBAL	instruments_2_supplied_name	String	NanoDrop spectrophotometer
attribute	NC_GLOBAL	instruments_3_dataset_instrument_description	String	an oxygen sensitive optode (Fibox 4, PreSens Gmbh, Regensburg, Germany).
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	708834
attribute	NC_GLOBAL	instruments_3_description	String	An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Optode
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	727
attribute	NC_GLOBAL	instruments_3_supplied_name	String	Oxygen sensitive optode
attribute	NC_GLOBAL	instruments_4_acronym	String	Thermal Cycler
attribute	NC_GLOBAL	instruments_4_dataset_instrument_description	String	AB-7500 real-time QPCR system [ThermoFisher (Applied Biosystems), Waltham, MA, USA], with the following cycling conditions: 94◦ C for 10 min, followed by 40 cycles of 94◦C for 15 s, 60◦C for 1 min, and 72◦C for 15 s.
attribute	NC_GLOBAL	instruments_4_dataset_instrument_nid	String	708845
attribute	NC_GLOBAL	instruments_4_description	String	General term for a laboratory apparatus commonly used for performing polymerase chain reaction (PCR). The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. (adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)
attribute	NC_GLOBAL	instruments_4_instrument_name	String	PCR Thermal Cycler
attribute	NC_GLOBAL	instruments_4_instrument_nid	String	471582
attribute	NC_GLOBAL	instruments_4_supplied_name	String	ThermoFisher QPCR system
attribute	NC_GLOBAL	instruments_5_dataset_instrument_description	String	Measured baseline absorbance at 412nm. (Fluostar Omega, BMG, Cary, NC, USA)
attribute	NC_GLOBAL	instruments_5_dataset_instrument_nid	String	743373
attribute	NC_GLOBAL	instruments_5_description	String	Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: https://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.
attribute	NC_GLOBAL	instruments_5_instrument_name	String	plate reader
attribute	NC_GLOBAL	instruments_5_instrument_nid	String	528693
attribute	NC_GLOBAL	instruments_5_supplied_name	String	microplate reader
attribute	NC_GLOBAL	keywords	String	activity, anemone, at96, bco, bco-dmo, biological, biomass, chemical, co1, data, dataset, dens, dmo, dna, erddap, fraction, genome, genome_ratio_AT96, genome_ratio_CO1, genome_ratio_CO1_Pro, holobiont, holobiont_resp_total, host, host_CS_activity, host_CS_activity_product_total, host_DNA_total, host_symb_dens_normal, management, normal, oceanography, office, preliminary, pro, product, prot, prot_normal_resp, protein, ratio, resp, respir, sample, symb, symb_biomass_fraction, symb_CS_activity, symb_respir_fraction, total, total_anemone_CS_activity, total_host_protein
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/649708/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/649708
attribute	NC_GLOBAL	param_mapping	String	{'649708': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/649708/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of Delaware
attribute	NC_GLOBAL	people_0_person_name	String	Mark E. Warner
attribute	NC_GLOBAL	people_0_person_nid	String	51387
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Ms Dicky Allison
attribute	NC_GLOBAL	people_1_person_nid	String	50382
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	AnemoneOA
attribute	NC_GLOBAL	projects_0_acronym	String	AnemoneOA
attribute	NC_GLOBAL	projects_0_description	String	The projected rise in carbon dioxide (CO2) in the atmosphere is considered a primary threat to marine systems throughout the world due to both ocean acidification and rising ocean temperatures. Coral reefs are very sensitive to these projected changes in the earth's climate, with continued losses in growth as well as disruption (also known as bleaching) in the symbiotic relationship between the algae (Symbiodinium) living within a diversity of host animals, including stony corals, soft corals and sea anemones. While much information has been gleaned as to how acidification may affect stony corals, considerably less is known about the interactive effects of acidification and temperature to other symbiotic anthozoans. To this end, this proposal will investigate the long-term impacts of elevated CO2 and temperature on the model sea anemone, Aiptasia pallida, while harboring four different genotypes of Symbiodinium. The primary goals of this project are (1) to determine the sensitivity and capacity for acclimation in molecular and physiological processes while exposed to elevated CO2 and temperature, and (2) to assess the degree to which acclimated adult animals may confer (or transfer) an imprinted physiological characteristic to the next generation of asexual offspring. A series of long-term experiments will be conducted with each animal/algal combination (holobiont) in order to collect initial (3 month) stress markers and genomic data and then follow animal response and asexual reproduction through several generations for one year. The possibility for enhanced resilience or acclimation will be measured by tracking the recovery of each holobiont, followed by repeated exposure to elevated temperature while held in high CO2. This project will tease apart fine scale mechanisms of stress, acclimation, or amelioration that may vary as a function of algal genotype and host animal response, and the degree to which environmental imprinting may pre-acclimate propagules. Project results will provide information regarding how future acidification and warming will affect cnidarian-algal symbioses, and the fundamental profile of their flexibility in stress response processes across organismal, metabolic, genomic and epigenetic scales.
attribute	NC_GLOBAL	projects_0_end_date	String	2016-06
attribute	NC_GLOBAL	projects_0_geolocation	String	Lewes, Delaware
attribute	NC_GLOBAL	projects_0_name	String	Ocean Acidification: Understanding the Impact of CO2 and Temperature on the Physiological, Genetic, and Epigenetic Response of a Model Sea Anemone System with Different Symbionts
attribute	NC_GLOBAL	projects_0_project_nid	String	628967
attribute	NC_GLOBAL	projects_0_start_date	String	2013-07
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Host-symbiont respiration related to symbiont density; anemones from Key Largo from (AnemoneOA project)
attribute	NC_GLOBAL	title	String	[Host-Symbiont respiration relationship] - Host-symbiont respiration related to symbiont density; anemones from Key Largo from (AnemoneOA project) (Ocean Acidification: Understanding the Impact of CO2 and Temperature on the Physiological, Genetic, and Epigenetic Response of a Model Sea Anemone System with Different Symbionts)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	sample		String
attribute	sample	bcodmo_name	String	sample
attribute	sample	description	String	sample ID number
attribute	sample	long_name	String	Sample
attribute	sample	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	sample	units	String	unitless
variable	holobiont_resp_total		float
attribute	holobiont_resp_total	_FillValue	float	NaN
attribute	holobiont_resp_total	actual_range	float	0.2603, 1.6616
attribute	holobiont_resp_total	bcodmo_name	String	respiration
attribute	holobiont_resp_total	description	String	total holobiont (anemone)respiration
attribute	holobiont_resp_total	long_name	String	Holobiont Resp Total
attribute	holobiont_resp_total	units	String	micromoles of oxygen per hour
variable	prot_normal_resp		float
attribute	prot_normal_resp	_FillValue	float	NaN
attribute	prot_normal_resp	actual_range	float	0.6121, 1.9819
attribute	prot_normal_resp	bcodmo_name	String	respiration
attribute	prot_normal_resp	description	String	Host protein-normalised holobiont respiration
attribute	prot_normal_resp	long_name	String	Prot Normal Resp
attribute	prot_normal_resp	units	String	micromoles of oxygen per hour per milligram protein
variable	host_CS_activity_product_total		float
attribute	host_CS_activity_product_total	_FillValue	float	NaN
attribute	host_CS_activity_product_total	actual_range	float	0.014, 0.1088
attribute	host_CS_activity_product_total	bcodmo_name	String	metabolic_enzyme
attribute	host_CS_activity_product_total	description	String	Total host citrate synthase activity; the product of host citrate synthase activity and total host protein
attribute	host_CS_activity_product_total	long_name	String	Host CS Activity Product Total
attribute	host_CS_activity_product_total	units	String	Units per milligram protein X milligrams
variable	host_CS_activity		float
attribute	host_CS_activity	_FillValue	float	NaN
attribute	host_CS_activity	actual_range	float	0.0343, 0.1794
attribute	host_CS_activity	bcodmo_name	String	metabolic_enzyme
attribute	host_CS_activity	description	String	Host citrate synthase specific activity
attribute	host_CS_activity	long_name	String	Host CS Activity
attribute	host_CS_activity	units	String	units per milligram protein
variable	total_host_protein		float
attribute	total_host_protein	_FillValue	float	NaN
attribute	total_host_protein	actual_range	float	0.1369, 2.1751
attribute	total_host_protein	bcodmo_name	String	unknown
attribute	total_host_protein	description	String	Total host protein
attribute	total_host_protein	long_name	String	Total Host Protein
attribute	total_host_protein	units	String	milligrams
variable	symb_CS_activity		float
attribute	symb_CS_activity	_FillValue	float	NaN
attribute	symb_CS_activity	actual_range	float	0.0334, 0.1677
attribute	symb_CS_activity	bcodmo_name	String	metabolic_enzyme
attribute	symb_CS_activity	description	String	Symbiont citrate synthase specific activity
attribute	symb_CS_activity	long_name	String	Symb CS Activity
attribute	symb_CS_activity	units	String	Units per milligram protein
variable	total_anemone_CS_activity		float
attribute	total_anemone_CS_activity	_FillValue	float	NaN
attribute	total_anemone_CS_activity	actual_range	float	0.0144, 0.1199
attribute	total_anemone_CS_activity	bcodmo_name	String	metabolic_enzyme
attribute	total_anemone_CS_activity	description	String	Total holobiont citrate synthase activity
attribute	total_anemone_CS_activity	long_name	String	Total Anemone CS Activity
attribute	total_anemone_CS_activity	units	String	Units
variable	host_DNA_total		double
attribute	host_DNA_total	_FillValue	double	NaN
attribute	host_DNA_total	actual_range	double	0.104517579, 18.21235118
attribute	host_DNA_total	bcodmo_name	String	unknown
attribute	host_DNA_total	description	String	Total host DNA content
attribute	host_DNA_total	long_name	String	Host DNA Total
attribute	host_DNA_total	units	String	micrograms
variable	host_symb_dens_normal		float
attribute	host_symb_dens_normal	_FillValue	float	NaN
attribute	host_symb_dens_normal	actual_range	float	1.0364, 13.3863
attribute	host_symb_dens_normal	bcodmo_name	String	abundance
attribute	host_symb_dens_normal	description	String	Host protein-normalised symbiont density
attribute	host_symb_dens_normal	long_name	String	Host Symb Dens Normal
attribute	host_symb_dens_normal	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P03/current/B070/
attribute	host_symb_dens_normal	units	String	million cells per mg protein
variable	symb_biomass_fraction		float
attribute	symb_biomass_fraction	_FillValue	float	NaN
attribute	symb_biomass_fraction	actual_range	float	0.0291, 0.2336
attribute	symb_biomass_fraction	bcodmo_name	String	unknown
attribute	symb_biomass_fraction	colorBarMaximum	double	1.0
attribute	symb_biomass_fraction	colorBarMinimum	double	0.0
attribute	symb_biomass_fraction	description	String	Symbiont biomass fraction (calculated as total symbiont protein [mg]/total host+symbiont protein [mg])
attribute	symb_biomass_fraction	long_name	String	Symb Biomass Fraction
attribute	symb_biomass_fraction	units	String	dimensionless
variable	symb_respir_fraction		float
attribute	symb_respir_fraction	_FillValue	float	NaN
attribute	symb_respir_fraction	actual_range	float	0.00665, 0.1981
attribute	symb_respir_fraction	bcodmo_name	String	unknown
attribute	symb_respir_fraction	colorBarMaximum	double	1.0
attribute	symb_respir_fraction	colorBarMinimum	double	0.0
attribute	symb_respir_fraction	description	String	Symbiont respiration fraction (calculated as total symbiont citrate synthase activity [U]/total host+symbiont citrate synthase activity [U])
attribute	symb_respir_fraction	long_name	String	Symb Respir Fraction
attribute	symb_respir_fraction	units	String	dimensionless
variable	genome_ratio_CO1		float
attribute	genome_ratio_CO1	_FillValue	float	NaN
attribute	genome_ratio_CO1	actual_range	float	20.2126, 406.0552
attribute	genome_ratio_CO1	bcodmo_name	String	unknown
attribute	genome_ratio_CO1	description	String	Mitochondrial:nuclear genome ratio calculated using CO1 (QiaAmp DNA Mini Kit)
attribute	genome_ratio_CO1	long_name	String	Genome Ratio CO1
attribute	genome_ratio_CO1	units	String	dimensionless
variable	genome_ratio_AT96		float
attribute	genome_ratio_AT96	_FillValue	float	NaN
attribute	genome_ratio_AT96	actual_range	float	13.1141, 395.0889
attribute	genome_ratio_AT96	bcodmo_name	String	unknown
attribute	genome_ratio_AT96	description	String	Mitochondrial:nuclear genome ratio calculated using ATP6 (QiaAmp DNA Mini Kit)
attribute	genome_ratio_AT96	long_name	String	Genome Ratio AT96
attribute	genome_ratio_AT96	units	String	dimensionless
variable	genome_ratio_CO1_Pro		float
attribute	genome_ratio_CO1_Pro	_FillValue	float	NaN
attribute	genome_ratio_CO1_Pro	actual_range	float	50.8763, 352.7607
attribute	genome_ratio_CO1_Pro	bcodmo_name	String	unknown
attribute	genome_ratio_CO1_Pro	description	String	Mitochondrial:nuclear genome ratio calculated using CO1 (Promega Wizard Kit)
attribute	genome_ratio_CO1_Pro	long_name	String	Genome Ratio CO1 Pro
attribute	genome_ratio_CO1_Pro	units	String	dimensionless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.