ERDDAP > info > bcodmo_dataset_649728

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Host-symbiont reinfections] - Symbiont removed and anemone reinfected with one of two strains of Symbiodinium from (AnemoneOA project) (Ocean Acidification: Understanding the Impact of CO2 and Temperature on the Physiological, Genetic, and Epigenetic Response of a Model Sea Anemone System with Different Symbionts)		I   M	background			BCO-DMO	bcodmo_dataset_649728

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Full details of the experimental design are provided in Hawkins et al., 2016
attribute	NC_GLOBAL	awards_0_award_nid	String	628966
attribute	NC_GLOBAL	awards_0_award_number	String	EF-1316055
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1316055
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Emerging Frontiers Division
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF EF
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	392
attribute	NC_GLOBAL	awards_0_program_manager	String	Irwin Forseth
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	520504
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Sea Anemone respiratory capacity with two different symbionts in an experimental setting CS = Citrate Synthase M. Warner, PI Version 14 June 16
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2016-06-17T15:05:21Z
attribute	NC_GLOBAL	date_modified	String	2019-07-02T15:06:36Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.649728.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/649728
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	[EVOS system, ThermoFisher (Life Technologies), Waltham, MA, USA; excitation: 628 ± 20 nm, emission: 692 ± 20 nm]. Field of view was determined using the EVOS operating software (4× objective), and cells were counted using the “Analyze Particles” tool in ImageJ (NIH, Bethesda, MD, USA).
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	652568
attribute	NC_GLOBAL	instruments_0_description	String	Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB06/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Microscope-Fluorescence
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	695
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Fluorescence Microscope
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	an oxygen sensitive optode (Fibox 4, PreSens Gmbh, Regensburg, Germany).
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	652570
attribute	NC_GLOBAL	instruments_1_description	String	An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Optode
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	727
attribute	NC_GLOBAL	instruments_1_supplied_name	String	oxygen sensitive optode
attribute	NC_GLOBAL	instruments_2_acronym	String	Thermal Cycler
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	AB-7500 real-time QPCR system [ThermoFisher (Applied Biosystems), Waltham, MA, USA], with the following cycling conditions: 94◦ C for 10 min, followed by 40 cycles of 94◦C for 15 s, 60◦C for 1 min, and 72◦C for 15 s.
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	652569
attribute	NC_GLOBAL	instruments_2_description	String	General term for a laboratory apparatus commonly used for performing polymerase chain reaction (PCR). The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. (adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)
attribute	NC_GLOBAL	instruments_2_instrument_name	String	PCR Thermal Cycler
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	471582
attribute	NC_GLOBAL	instruments_2_supplied_name	String	ThermoFisher QPCR system
attribute	NC_GLOBAL	keywords	String	act, anemone, anemone_resp_rate, bco, bco-dmo, biological, biom, biomass, chemical, data, dataset, density, dmo, erddap, fraction, host, host_CS_act, management, oceanography, office, output, photo, photo_output_symb, preliminary, quotient, rate, resp, resp_biom_quotient, sample, symb, symbio, symbio_biomass_fraction, symbio_density, symbio_resp_fraction, symbiont, symbiont_CS_act, symbiont_id
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/649728/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/649728
attribute	NC_GLOBAL	param_mapping	String	{'649728': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/649728/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of Delaware
attribute	NC_GLOBAL	people_0_person_name	String	Mark E. Warner
attribute	NC_GLOBAL	people_0_person_nid	String	51387
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Ms Dicky Allison
attribute	NC_GLOBAL	people_1_person_nid	String	50382
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	AnemoneOA
attribute	NC_GLOBAL	projects_0_acronym	String	AnemoneOA
attribute	NC_GLOBAL	projects_0_description	String	The projected rise in carbon dioxide (CO2) in the atmosphere is considered a primary threat to marine systems throughout the world due to both ocean acidification and rising ocean temperatures. Coral reefs are very sensitive to these projected changes in the earth's climate, with continued losses in growth as well as disruption (also known as bleaching) in the symbiotic relationship between the algae (Symbiodinium) living within a diversity of host animals, including stony corals, soft corals and sea anemones. While much information has been gleaned as to how acidification may affect stony corals, considerably less is known about the interactive effects of acidification and temperature to other symbiotic anthozoans. To this end, this proposal will investigate the long-term impacts of elevated CO2 and temperature on the model sea anemone, Aiptasia pallida, while harboring four different genotypes of Symbiodinium. The primary goals of this project are (1) to determine the sensitivity and capacity for acclimation in molecular and physiological processes while exposed to elevated CO2 and temperature, and (2) to assess the degree to which acclimated adult animals may confer (or transfer) an imprinted physiological characteristic to the next generation of asexual offspring. A series of long-term experiments will be conducted with each animal/algal combination (holobiont) in order to collect initial (3 month) stress markers and genomic data and then follow animal response and asexual reproduction through several generations for one year. The possibility for enhanced resilience or acclimation will be measured by tracking the recovery of each holobiont, followed by repeated exposure to elevated temperature while held in high CO2. This project will tease apart fine scale mechanisms of stress, acclimation, or amelioration that may vary as a function of algal genotype and host animal response, and the degree to which environmental imprinting may pre-acclimate propagules. Project results will provide information regarding how future acidification and warming will affect cnidarian-algal symbioses, and the fundamental profile of their flexibility in stress response processes across organismal, metabolic, genomic and epigenetic scales.
attribute	NC_GLOBAL	projects_0_end_date	String	2016-06
attribute	NC_GLOBAL	projects_0_geolocation	String	Lewes, Delaware
attribute	NC_GLOBAL	projects_0_name	String	Ocean Acidification: Understanding the Impact of CO2 and Temperature on the Physiological, Genetic, and Epigenetic Response of a Model Sea Anemone System with Different Symbionts
attribute	NC_GLOBAL	projects_0_project_nid	String	628967
attribute	NC_GLOBAL	projects_0_start_date	String	2013-07
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Symbiont removed and anemone reinfected with one of two strains of Symbiodinium from (AnemoneOA project)
attribute	NC_GLOBAL	title	String	[Host-symbiont reinfections] - Symbiont removed and anemone reinfected with one of two strains of Symbiodinium from (AnemoneOA project) (Ocean Acidification: Understanding the Impact of CO2 and Temperature on the Physiological, Genetic, and Epigenetic Response of a Model Sea Anemone System with Different Symbionts)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	sample		String
attribute	sample	bcodmo_name	String	sample
attribute	sample	description	String	Sample ID number
attribute	sample	long_name	String	Sample
attribute	sample	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	sample	units	String	unitless
variable	symbiont_id		String
attribute	symbiont_id	bcodmo_name	String	taxon
attribute	symbiont_id	description	String	Symbiont identity; i.e. Homologous ITS2-type A4 Symbiodinium or Symbiodinium minutum ITS2-type B1
attribute	symbiont_id	long_name	String	Symbiont Id
attribute	symbiont_id	units	String	text
variable	anemone_resp_rate		double
attribute	anemone_resp_rate	_FillValue	double	NaN
attribute	anemone_resp_rate	actual_range	double	0.813157803, 2.214224466
attribute	anemone_resp_rate	bcodmo_name	String	O2 consumption
attribute	anemone_resp_rate	description	String	Host protein-normalised holobiont respiration rate
attribute	anemone_resp_rate	long_name	String	Anemone Resp Rate
attribute	anemone_resp_rate	units	String	micromoles of oxygen per hour per milligram protein
variable	host_CS_act		double
attribute	host_CS_act	_FillValue	double	NaN
attribute	host_CS_act	actual_range	double	0.06816797, 0.185088974
attribute	host_CS_act	bcodmo_name	String	metabolic_enzyme
attribute	host_CS_act	description	String	Host (anemone) citrate synthase specific activity
attribute	host_CS_act	long_name	String	Host CS Act
attribute	host_CS_act	units	String	Units per milligram protein
variable	symbiont_CS_act		double
attribute	symbiont_CS_act	_FillValue	double	NaN
attribute	symbiont_CS_act	actual_range	double	0.009343539, 0.13640119
attribute	symbiont_CS_act	bcodmo_name	String	metabolic_enzyme
attribute	symbiont_CS_act	description	String	Symbiont (Symbiodinium) citrate synthase specific activity
attribute	symbiont_CS_act	long_name	String	Symbiont CS Act
attribute	symbiont_CS_act	units	String	Units per milligram protein
variable	photo_output_symb		double
attribute	photo_output_symb	_FillValue	double	NaN
attribute	photo_output_symb	actual_range	double	0.184067192, 1.337695877
attribute	photo_output_symb	bcodmo_name	String	photosynthesis
attribute	photo_output_symb	description	String	Net photosynthesis per symbiont cell
attribute	photo_output_symb	long_name	String	Photo Output Symb
attribute	photo_output_symb	units	String	picomoles oxygen per hour per cell
variable	symbio_density		double
attribute	symbio_density	_FillValue	double	NaN
attribute	symbio_density	actual_range	double	1098543.993, 8812379.853
attribute	symbio_density	bcodmo_name	String	abundance
attribute	symbio_density	description	String	Host protein-normalised symbiont density
attribute	symbio_density	long_name	String	Symbio Density
attribute	symbio_density	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P03/current/B070/
attribute	symbio_density	units	String	million cells per mg protein
variable	symbio_biomass_fraction		float
attribute	symbio_biomass_fraction	_FillValue	float	NaN
attribute	symbio_biomass_fraction	actual_range	float	0.0183, 0.1581
attribute	symbio_biomass_fraction	bcodmo_name	String	unknown
attribute	symbio_biomass_fraction	colorBarMaximum	double	1.0
attribute	symbio_biomass_fraction	colorBarMinimum	double	0.0
attribute	symbio_biomass_fraction	description	String	Symbiont biomass fraction (calculated as total symbiont protein [mg]/total host+symbiont protein [mg])
attribute	symbio_biomass_fraction	long_name	String	Symbio Biomass Fraction
attribute	symbio_biomass_fraction	units	String	milligrams per milligrams(dimensionless)
variable	symbio_resp_fraction		float
attribute	symbio_resp_fraction	_FillValue	float	NaN
attribute	symbio_resp_fraction	actual_range	float	0.0041, 0.1433
attribute	symbio_resp_fraction	bcodmo_name	String	unknown
attribute	symbio_resp_fraction	colorBarMaximum	double	1.0
attribute	symbio_resp_fraction	colorBarMinimum	double	0.0
attribute	symbio_resp_fraction	description	String	Symbiont respiration fraction (calculated as total symbiont citrate synthase activity [U]/total host+symbiont citrate synthase activity [U])
attribute	symbio_resp_fraction	long_name	String	Symbio Resp Fraction
attribute	symbio_resp_fraction	units	String	dimensionless
variable	resp_biom_quotient		double
attribute	resp_biom_quotient	_FillValue	double	NaN
attribute	resp_biom_quotient	actual_range	double	0.072127984, 1.036789977
attribute	resp_biom_quotient	bcodmo_name	String	unknown
attribute	resp_biom_quotient	description	String	Symbiont respiration fraction/Symbiont biomass fraction
attribute	resp_biom_quotient	long_name	String	Resp Biom Quotient
attribute	resp_biom_quotient	units	String	dimensionless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.