Accessing BCO-DMO data
log in    
Brought to you by BCO-DMO    

ERDDAP > info > bcodmo_dataset_651461

Title Sum-
Institution Dataset ID
   set  data   graph     files  public Laboratory study of long-term growth in juvenile Menidia menidia (Atlantic silverside) at
contrasting CO2 levels for 16 to 122 days in 2015
   ?     I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_651461

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Field sampling and experimental design: Experiments were performed at
University of Connecticut\u2019s Avery Point Campus in the Rankin Laboratory,
a seawater facility adjacent to eastern Long Island Sound. Ripe adult M.
menidia were collected on 1 May 2015 from Mumford Cove (41\u00a019.25\u2019 N,
72\u00a01.09\u2019W), a shallow embayment dominated by eelgrasses (Zostera
marina) and open to the Long Island Sound. Adults were sampled with a 30 m
\u00d7 2 m beach seine, separated by sex, transported live to our laboratory,
and held for 48 hours in large aerated tanks (17 degrees celcius, ambient CO2,
no food). On the day of fertilization (3 May 2015), greater than or equal
to\u00a020 ripe individuals from each sex were strip-spawned and eggs evenly
distributed onto window screens (1 mm fiberglass mesh) submerged in plastic
dishes with clear seawater. Strip-spawned adults were measured for standard
length (mean SL, lower 0.5 cm; females 9.7, males 8.7). Fertilized embryos
quickly attach to the screens via chorionic filaments, which facilitates
precise enumeration and even allotment to treatments and replicates. Following
established protocols for rearing M. menidia offspring (Murray et al., 2014),
replicate containers (20 l) were filled with filtered (to 1 um) and UV
sterilized seawater (31 psu) from Long Island Sound and placed in water baths
(~300 l) controlled for temperature and light conditions (17 degrees celcius,
15h light:9h dark) throughout the duration of the experiment. Within 2h of
fertilization, each of four replicates per treatment received exactly 200
embryos for measure early life survival, while four other replicates per
treatment each received ~400 offspring for long-term rearing. Larvae hatched
~14 days post-fertilization (dpf) and were immediately provided with
standardized rations of newly hatched brine shrimp nauplii Artemia salina (San
Francisco strain, Brine Shrimp Direct) and a commercial larval powder food
(first four days, Otohime Marine Weaning Diet, size A, Reed Mariculture). At 2
days post-hatch (dph), living larvae from survival replicates were counted by
gently scooping small groups into replacement containers. Between 1 to 14 days
post-hatch (dph), all containers were cleaned daily with partial (10%) water

At 16 dph, larvae from the survival replicates were counted and a sub-sample
(Ncontrol = 37, Nhigh = 33) was preserved in 10% formaldehyde/seawater
solution for later total length (TL) measurements (nearest 0.01 mm) via
calibrated, digital images (ImagePro Premier\u00a0V9.1). All surviving larvae
were transferred to larger (50 l) tubs and maintained under the previously
described protocol. At 33 dph, larvae from the survival replicates were
counted and then all larvae transferred to 50 l tubs fitted with screen-
covered holes (1 mm mesh) to promote water exchange from a 300 l seawater
bath. Due to space constraints, from 33 to 54 dph larvae from the survival
replicates were pooled into a single container per CO2 treatment. Larvae were
provided rations of nauplii and supplemented with commercial powder food
(Otohime B1, Reed Mariculture). At 54 dph, all juveniles from survival and
grow-out replicates were counted and pooled at equal numbers into 300 l circle
tanks (two tanks per treatment, ~615 fish per tank). Juveniles were provided
equal rations of newly hatched nauplii and B1 commercial powder food. Tanks
were siphoned for waste daily and partial water changes completed twice
weekly. Additional sub-samples for length measurements (TL, nearest 0.01 mm)
were made at 36 dph (Ncontrol = 20, Nhigh = 20), 68 dph (Ncontrol = 20, Nhigh
= 20) and 100 dph (Ncontrol = 28, Nhigh = 28).

At 122 dph, the experiment was terminated and all surviving juveniles were
euthanized via an overdose of Tricaine-S (MS 222, Western Chemical) for
preservation. While some juveniles from each treatment were immediately frozen
at -80 degrees celcius\u00a0for fatty acid analyses; ~75% of the samples were
fixed in 10% buffered formaldehyde/seawater solution for TL (Ncontrol = 1,025;
Nhigh = 1,100, nearest 0.01 mm) and weight measurements (Ncontrol = 720; Nhigh
= 786, nearest 0.01g).\u00a0
attribute NC_GLOBAL awards_0_award_nid String 650183
attribute NC_GLOBAL awards_0_award_number String OCE-1536336
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1536336 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Long-term Menidia growth
H.Baumann, J.Nye, PIs
Version 7 July 2016
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2016-07-07T20:53:22Z
attribute NC_GLOBAL date_modified String 2017-11-15T20:19:17Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.652124
attribute NC_GLOBAL Easternmost_Easting double -72.01483611
attribute NC_GLOBAL geospatial_lat_max double 41.32133889
attribute NC_GLOBAL geospatial_lat_min double 41.32133889
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -72.01483611
attribute NC_GLOBAL geospatial_lon_min double -72.01483611
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/651461 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String pH Sensor
attribute NC_GLOBAL instruments_0_dataset_instrument_description String handheld pH probe
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 651464
attribute NC_GLOBAL instruments_0_description String General term for an instrument that measures the pH or how acidic or basic a solution is.
attribute NC_GLOBAL instruments_0_instrument_name String pH Sensor
attribute NC_GLOBAL instruments_0_instrument_nid String 674
attribute NC_GLOBAL instruments_0_supplied_name String Orion ROSS Ultra pH/ATC Triode
attribute NC_GLOBAL instruments_1_acronym String pH Sensor
attribute NC_GLOBAL instruments_1_dataset_instrument_description String portable pH meter
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 651470
attribute NC_GLOBAL instruments_1_description String General term for an instrument that measures the pH or how acidic or basic a solution is.
attribute NC_GLOBAL instruments_1_instrument_name String pH Sensor
attribute NC_GLOBAL instruments_1_instrument_nid String 674
attribute NC_GLOBAL instruments_1_supplied_name String Orion Star A121 pH Portable Meter
attribute NC_GLOBAL instruments_2_acronym String Automatic titrator
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Potentiometric Titrator
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 651471
attribute NC_GLOBAL instruments_2_description String Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB12/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Automatic titrator
attribute NC_GLOBAL instruments_2_instrument_nid String 682
attribute NC_GLOBAL instruments_2_supplied_name String Mettler Toledo G20
attribute NC_GLOBAL keywords String adult, adult_collection_site, age, bco, bco-dmo, biological, carbon, carbon dioxide, chemical, co2, CO2_treatment, collection, condition, condition_factor, data, dataset, date, date_sample, dioxide, dmo, erddap, factor, fertilization, latitude, length, length_total, longitude, management, oceanography, office, pH_treatment, preliminary, sample, site, species, tank, temperature, time, total, treatment, weight, wet, wet_weight
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/651461/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/651461 (external link)
attribute NC_GLOBAL Northernmost_Northing double 41.32133889
attribute NC_GLOBAL param_mapping String {'651461': {'lat': 'master - latitude', 'lon': 'master - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/651461/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Connecticut
attribute NC_GLOBAL people_0_affiliation_acronym String UConn
attribute NC_GLOBAL people_0_person_name String Hannes Baumann
attribute NC_GLOBAL people_0_person_nid String 528586
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Stony Brook University - SoMAS
attribute NC_GLOBAL people_1_affiliation_acronym String SUNY-SB SoMAS
attribute NC_GLOBAL people_1_person_name String Janet Nye
attribute NC_GLOBAL people_1_person_nid String 650186
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of Connecticut
attribute NC_GLOBAL people_2_affiliation_acronym String UConn
attribute NC_GLOBAL people_2_person_name String Hannes Baumann
attribute NC_GLOBAL people_2_person_nid String 528586
attribute NC_GLOBAL people_2_role String Contact
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Hannah Ake
attribute NC_GLOBAL people_3_person_nid String 650173
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL people_4_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_4_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_4_person_name String Ms Dicky Allison
attribute NC_GLOBAL people_4_person_nid String 50382
attribute NC_GLOBAL people_4_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_4_role_type String related
attribute NC_GLOBAL project String HYPOA
attribute NC_GLOBAL projects_0_acronym String HYPOA
attribute NC_GLOBAL projects_0_description String Description from NSF award abstract:
Coastal marine ecosystems provide a number of important services and resources for humans, and at the same time, coastal waters are subject to environmental stressors such as increases in ocean acidification and reductions in dissolved oxygen. The effects of these stressors on coastal marine organisms remain poorly understood because most research to date has examined the sensitivity of species to one factor, but not to more than one in combination. This project will determine how a model fish species, the Atlantic silverside, will respond to observed and predicted levels of dissolved carbon dioxide (CO2) and oxygen (O2). Shorter-term experiments will measure embryo and larval survival, growth, and metabolism, and determine whether parents experiencing stressful conditions produce more robust offspring. Longer-term experiments will study the consequences of ocean acidification over the entire life span by quantifying the effects of high-CO2 conditions on the ratio of males to females, lifetime growth, and reproductive investment. These studies will provide a more comprehensive view of how multiple stressors may impact populations of Atlantic silversides and potentially other important forage fish species. This collaborative project will support and train three graduate students at the University of Connecticut and the Stony Brook University (NY), two institutions that attract students from minority groups. It will also provide a variety of opportunities for undergraduates to participate in research and the public to learn about the study, through summer research projects, incorporation in the "Women in Science and Engineering" program, and interactive displays of environmental data from monitoring buoys. The two early-career investigators are committed to increasing ocean literacy and awareness of NSF-funded research through public talks and presentations.
This project responds to the recognized need for multi-stressor assessments of species sensitivities to anthropogenic environmental change. It will combine environmental monitoring with advanced experimental approaches to characterize early and whole life consequences of acidification and hypoxia in the Atlantic silverside (Menidia menidia), a valued model species and important forage fish along most of the US east coast. Experiments will employ a newly constructed, computer-controlled fish rearing system to allow independent and combined manipulation of seawater pCO2 and dissolved oxygen (DO) content and the application of static and fluctuating pCO2 and DO levels that were chosen to represent contemporary and potential future scenarios in productive coastal habitats. First CO2, DO, and CO2 × DO dependent reaction norms will be quantified for fitness-relevant early life history (ELH) traits including pre- and post-hatch survival, time to hatch, post-hatch growth, by rearing offspring collected from wild adults from fertilization to 20 days post hatch (dph) using a full factorial design of 3 CO2 × 3 DO levels. Second, the effects of tidal and diel CO2 × DO fluctuations of different amplitudes on silverside ELH traits will be quantified. To address knowledge gaps regarding the CO2-sensitivity in this species, laboratory manipulations of adult spawner environments and reciprocal offspring exposure experiments will elucidate the role of transgenerational plasticity as a potential short-term mechanism to cope with changing environments. To better understand the mechanisms of fish early life CO2-sensitivity, the effects of temperature × CO2 on pre- and post-hatch metabolism will be robustly quantified. The final objective is to rear silversides from fertilization to maturity under different CO2 levels and assess potential CO2-effects on sex ratio and whole life growth and fecundity.
Related references:
Gobler, C.J. and Baumann, H. (2016) Hypoxia and acidification in ocean ecosystems: Coupled dynamics and effects on marine life. Biology Letters 12:20150976. doi:10.1098/rsbl.2015.0976
Baumann, H. (2016) Combined effects of ocean acidification, warming, and hypoxia on marine organisms. Limnology and Oceanography e-Lectures 6:1-43. doi:10.1002/loe2.10002
Depasquale, E., Baumann, H., and Gobler, C.J. (2015) Variation in early life stage vulnerability among Northwest Atlantic estuarine forage fish to ocean acidification and low oxygen Marine Ecology Progress Series 523: 145–156.doi:10.3354/meps11142
attribute NC_GLOBAL projects_0_end_date String 2018-11
attribute NC_GLOBAL projects_0_geolocation String Eastern Long Island Sound, CT, USA
attribute NC_GLOBAL projects_0_name String Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish
attribute NC_GLOBAL projects_0_project_nid String 650184
attribute NC_GLOBAL projects_0_start_date String 2015-12
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 41.32133889
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String species,adult_collection_site,latitude,longitude,temp
attribute NC_GLOBAL summary String Total length, wet weight, and condition factor of juvenile
Menidia\u00a0menidia reared at contrasting CO2 levels.

These data are\u00a0associated with the corresponding paper:

Murray, C.S., Fuiman, L., and Baumann, H.\u00a0Consequences of elevated
CO2\u00a0exposure across multiple life stages in a coastal forage
fish.\u00a0ICES Journal of Marine Science\u00a074:1051-1061(2017).
attribute NC_GLOBAL title String Laboratory study of long-term growth in juvenile Menidia menidia (Atlantic silverside) at contrasting CO2 levels for 16 to 122 days in 2015
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -72.01483611
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable species   String  
attribute species bcodmo_name String species
attribute species description String Menidia menidia; the Atlantic silverside
attribute species long_name String Species
attribute species units String unitless
variable adult_collection_site   String  
attribute adult_collection_site bcodmo_name String site
attribute adult_collection_site description String site of the collection of wild adult spawners on 1 May 2015
attribute adult_collection_site long_name String Adult Collection Site
attribute adult_collection_site units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 41.32133889, 41.32133889
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String latitude of the adult collection site
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -72.01483611, -72.01483611
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String longitude of the adult collections site
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable date_fertilization   String  
attribute date_fertilization bcodmo_name String date
attribute date_fertilization description String date offspring were fertilized by strip-spawning; over twenty adults of each sex; format mm-dd-YYYY
attribute date_fertilization long_name String Date Fertilization
attribute date_fertilization nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute date_fertilization source_name String date_fertilization
attribute date_fertilization time_precision String 1970-01-01
attribute date_fertilization units String unitless
variable age   byte  
attribute age _FillValue byte 127
attribute age actual_range byte 16, 122
attribute age bcodmo_name String age
attribute age description String age in days post hatch (dph) on the date of sampling
attribute age long_name String Age
attribute age units String days
variable date_sample   String  
attribute date_sample bcodmo_name String date
attribute date_sample description String date offspring were sampled for length and weight measurements; format mm-dd-YYYY
attribute date_sample long_name String Date Sample
attribute date_sample nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute date_sample time_precision String 1970-01-01
attribute date_sample units String unitless
variable temp   byte  
attribute temp _FillValue byte 127
attribute temp actual_range byte 17, 17
attribute temp bcodmo_name String temperature
attribute temp description String seventeen degrees celcius; all fish experienced the same temperature
attribute temp long_name String Temperature
attribute temp nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/ (external link)
attribute temp units String degrees celsius
variable pH_treatment   float  
attribute pH_treatment _FillValue float NaN
attribute pH_treatment actual_range float 7.45, 8.05
attribute pH_treatment bcodmo_name String pH
attribute pH_treatment description String average pH levels experienced by the fish; 7.45 or 8.05
attribute pH_treatment long_name String P H Treatment
attribute pH_treatment nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute pH_treatment units String dimensionless
variable CO2_treatment   short  
attribute CO2_treatment _FillValue short 32767
attribute CO2_treatment actual_range short 500, 2150
attribute CO2_treatment bcodmo_name String pCO2
attribute CO2_treatment description String calculated pCO2 levels; calculated by CO2SYS; based on alkalinity pH and temperature
attribute CO2_treatment long_name String CO2 Treatment
attribute CO2_treatment nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/ (external link)
attribute CO2_treatment units String microatomosphere
variable length_total   float  
attribute length_total _FillValue float NaN
attribute length_total actual_range float 6.15, 59.4
attribute length_total bcodmo_name String fish_len
attribute length_total description String total length of the fish at sampling
attribute length_total long_name String Length Total
attribute length_total units String millimeter
variable wet_weight   short  
attribute wet_weight _FillValue short 32767
attribute wet_weight actual_range short 30, 750
attribute wet_weight bcodmo_name String weight
attribute wet_weight description String wet weight of the fish at sampling
attribute wet_weight long_name String Wet Weight
attribute wet_weight units String milligram
variable condition_factor   float  
attribute condition_factor _FillValue float NaN
attribute condition_factor actual_range float 0.3317, 0.9544
attribute condition_factor bcodmo_name String unknown
attribute condition_factor description String condition factor calculated as wetWeight/totalLength to the 2.734608 power
attribute condition_factor long_name String Condition Factor
attribute condition_factor units String dimensionless
variable tank   String  
attribute tank bcodmo_name String tank
attribute tank description String tank number; A B C D
attribute tank long_name String Tank
attribute tank units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

ERDDAP, Version 2.02
Disclaimers | Privacy Policy | Contact