ERDDAP > info > bcodmo_dataset_651461

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
	set	data	graph		files	public	Laboratory study of long-term growth in juvenile Menidia menidia (Atlantic silverside) at contrasting CO2 levels for 16 to 122 days in 2015		I   M	background			BCO-DMO	bcodmo_dataset_651461

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute	NC_GLOBAL	acquisition_description	String	Field sampling and experimental design: Experiments were performed at University of Connecticut\u2019s Avery Point Campus in the Rankin Laboratory, a seawater facility adjacent to eastern Long Island Sound. Ripe adult M. menidia were collected on 1 May 2015 from Mumford Cove (41\u00a019.25\u2019 N, 72\u00a01.09\u2019W), a shallow embayment dominated by eelgrasses (Zostera marina) and open to the Long Island Sound. Adults were sampled with a 30 m \u00d7 2 m beach seine, separated by sex, transported live to our laboratory, and held for 48 hours in large aerated tanks (17 degrees celcius, ambient CO2, no food). On the day of fertilization (3 May 2015), greater than or equal to\u00a020 ripe individuals from each sex were strip-spawned and eggs evenly distributed onto window screens (1 mm fiberglass mesh) submerged in plastic dishes with clear seawater. Strip-spawned adults were measured for standard length (mean SL, lower 0.5 cm; females 9.7, males 8.7). Fertilized embryos quickly attach to the screens via chorionic filaments, which facilitates precise enumeration and even allotment to treatments and replicates. Following established protocols for rearing M. menidia offspring (Murray et al., 2014), replicate containers (20 l) were filled with filtered (to 1 um) and UV sterilized seawater (31 psu) from Long Island Sound and placed in water baths (~300 l) controlled for temperature and light conditions (17 degrees celcius, 15h light:9h dark) throughout the duration of the experiment. Within 2h of fertilization, each of four replicates per treatment received exactly 200 embryos for measure early life survival, while four other replicates per treatment each received ~400 offspring for long-term rearing. Larvae hatched ~14 days post-fertilization (dpf) and were immediately provided with standardized rations of newly hatched brine shrimp nauplii Artemia salina (San Francisco strain, Brine Shrimp Direct) and a commercial larval powder food (first four days, Otohime Marine Weaning Diet, size A, Reed Mariculture). At 2 days post-hatch (dph), living larvae from survival replicates were counted by gently scooping small groups into replacement containers. Between 1 to 14 days post-hatch (dph), all containers were cleaned daily with partial (10%) water exchange. At 16 dph, larvae from the survival replicates were counted and a sub-sample (Ncontrol = 37, Nhigh = 33) was preserved in 10% formaldehyde/seawater solution for later total length (TL) measurements (nearest 0.01 mm) via calibrated, digital images (ImagePro Premier\u00a0V9.1). All surviving larvae were transferred to larger (50 l) tubs and maintained under the previously described protocol. At 33 dph, larvae from the survival replicates were counted and then all larvae transferred to 50 l tubs fitted with screen- covered holes (1 mm mesh) to promote water exchange from a 300 l seawater bath. Due to space constraints, from 33 to 54 dph larvae from the survival replicates were pooled into a single container per CO2 treatment. Larvae were provided rations of nauplii and supplemented with commercial powder food (Otohime B1, Reed Mariculture). At 54 dph, all juveniles from survival and grow-out replicates were counted and pooled at equal numbers into 300 l circle tanks (two tanks per treatment, ~615 fish per tank). Juveniles were provided equal rations of newly hatched nauplii and B1 commercial powder food. Tanks were siphoned for waste daily and partial water changes completed twice weekly. Additional sub-samples for length measurements (TL, nearest 0.01 mm) were made at 36 dph (Ncontrol = 20, Nhigh = 20), 68 dph (Ncontrol = 20, Nhigh = 20) and 100 dph (Ncontrol = 28, Nhigh = 28). At 122 dph, the experiment was terminated and all surviving juveniles were euthanized via an overdose of Tricaine-S (MS 222, Western Chemical) for preservation. While some juveniles from each treatment were immediately frozen at -80 degrees celcius\u00a0for fatty acid analyses; ~75% of the samples were fixed in 10% buffered formaldehyde/seawater solution for TL (Ncontrol = 1,025; Nhigh = 1,100, nearest 0.01 mm) and weight measurements (Ncontrol = 720; Nhigh = 786, nearest 0.01g).\u00a0
attribute	NC_GLOBAL	awards_0_award_nid	String	650183
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1536336
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1536336
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Michael E. Sieracki
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50446
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Long-term Menidia growth H.Baumann, J.Nye, PIs Version 7 July 2016
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2016-07-07T20:53:22Z
attribute	NC_GLOBAL	date_modified	String	2017-11-15T20:19:17Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.652124
attribute	NC_GLOBAL	Easternmost_Easting	double	-72.01483611
attribute	NC_GLOBAL	geospatial_lat_max	double	41.32133889
attribute	NC_GLOBAL	geospatial_lat_min	double	41.32133889
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	-72.01483611
attribute	NC_GLOBAL	geospatial_lon_min	double	-72.01483611
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/651461
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	pH Sensor
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	handheld pH probe
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	651464
attribute	NC_GLOBAL	instruments_0_description	String	General term for an instrument that measures the pH or how acidic or basic a solution is.
attribute	NC_GLOBAL	instruments_0_instrument_name	String	pH Sensor
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	674
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Orion ROSS Ultra pH/ATC Triode
attribute	NC_GLOBAL	instruments_1_acronym	String	pH Sensor
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	portable pH meter
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	651470
attribute	NC_GLOBAL	instruments_1_description	String	General term for an instrument that measures the pH or how acidic or basic a solution is.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	pH Sensor
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	674
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Orion Star A121 pH Portable Meter
attribute	NC_GLOBAL	instruments_2_acronym	String	Automatic titrator
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Potentiometric Titrator
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	651471
attribute	NC_GLOBAL	instruments_2_description	String	Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.
attribute	NC_GLOBAL	instruments_2_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB12/
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Automatic titrator
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	682
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Mettler Toledo G20
attribute	NC_GLOBAL	keywords	String	adult, adult_collection_site, age, bco, bco-dmo, biological, carbon, carbon dioxide, chemical, co2, CO2_treatment, collection, condition, condition_factor, data, dataset, date, date_sample, dioxide, dmo, erddap, factor, fertilization, latitude, length, length_total, longitude, management, oceanography, office, pH_treatment, preliminary, sample, site, species, tank, temperature, time, total, treatment, weight, wet, wet_weight
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/651461/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/651461
attribute	NC_GLOBAL	Northernmost_Northing	double	41.32133889
attribute	NC_GLOBAL	param_mapping	String	{'651461': {'lat': 'master - latitude', 'lon': 'master - longitude'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/651461/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of Connecticut
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UConn
attribute	NC_GLOBAL	people_0_person_name	String	Hannes Baumann
attribute	NC_GLOBAL	people_0_person_nid	String	528586
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Stony Brook University - SoMAS
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	SUNY-SB SoMAS
attribute	NC_GLOBAL	people_1_person_name	String	Janet Nye
attribute	NC_GLOBAL	people_1_person_nid	String	650186
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	University of Connecticut
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	UConn
attribute	NC_GLOBAL	people_2_person_name	String	Hannes Baumann
attribute	NC_GLOBAL	people_2_person_nid	String	528586
attribute	NC_GLOBAL	people_2_role	String	Contact
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	people_3_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_3_person_name	String	Hannah Ake
attribute	NC_GLOBAL	people_3_person_nid	String	650173
attribute	NC_GLOBAL	people_3_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	people_4_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_4_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_4_person_name	String	Ms Dicky Allison
attribute	NC_GLOBAL	people_4_person_nid	String	50382
attribute	NC_GLOBAL	people_4_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_4_role_type	String	related
attribute	NC_GLOBAL	project	String	HYPOA
attribute	NC_GLOBAL	projects_0_acronym	String	HYPOA
attribute	NC_GLOBAL	projects_0_description	String	Description from NSF award abstract: Coastal marine ecosystems provide a number of important services and resources for humans, and at the same time, coastal waters are subject to environmental stressors such as increases in ocean acidification and reductions in dissolved oxygen. The effects of these stressors on coastal marine organisms remain poorly understood because most research to date has examined the sensitivity of species to one factor, but not to more than one in combination. This project will determine how a model fish species, the Atlantic silverside, will respond to observed and predicted levels of dissolved carbon dioxide (CO2) and oxygen (O2). Shorter-term experiments will measure embryo and larval survival, growth, and metabolism, and determine whether parents experiencing stressful conditions produce more robust offspring. Longer-term experiments will study the consequences of ocean acidification over the entire life span by quantifying the effects of high-CO2 conditions on the ratio of males to females, lifetime growth, and reproductive investment. These studies will provide a more comprehensive view of how multiple stressors may impact populations of Atlantic silversides and potentially other important forage fish species. This collaborative project will support and train three graduate students at the University of Connecticut and the Stony Brook University (NY), two institutions that attract students from minority groups. It will also provide a variety of opportunities for undergraduates to participate in research and the public to learn about the study, through summer research projects, incorporation in the "Women in Science and Engineering" program, and interactive displays of environmental data from monitoring buoys. The two early-career investigators are committed to increasing ocean literacy and awareness of NSF-funded research through public talks and presentations. This project responds to the recognized need for multi-stressor assessments of species sensitivities to anthropogenic environmental change. It will combine environmental monitoring with advanced experimental approaches to characterize early and whole life consequences of acidification and hypoxia in the Atlantic silverside (Menidia menidia), a valued model species and important forage fish along most of the US east coast. Experiments will employ a newly constructed, computer-controlled fish rearing system to allow independent and combined manipulation of seawater pCO2 and dissolved oxygen (DO) content and the application of static and fluctuating pCO2 and DO levels that were chosen to represent contemporary and potential future scenarios in productive coastal habitats. First CO2, DO, and CO2 × DO dependent reaction norms will be quantified for fitness-relevant early life history (ELH) traits including pre- and post-hatch survival, time to hatch, post-hatch growth, by rearing offspring collected from wild adults from fertilization to 20 days post hatch (dph) using a full factorial design of 3 CO2 × 3 DO levels. Second, the effects of tidal and diel CO2 × DO fluctuations of different amplitudes on silverside ELH traits will be quantified. To address knowledge gaps regarding the CO2-sensitivity in this species, laboratory manipulations of adult spawner environments and reciprocal offspring exposure experiments will elucidate the role of transgenerational plasticity as a potential short-term mechanism to cope with changing environments. To better understand the mechanisms of fish early life CO2-sensitivity, the effects of temperature × CO2 on pre- and post-hatch metabolism will be robustly quantified. The final objective is to rear silversides from fertilization to maturity under different CO2 levels and assess potential CO2-effects on sex ratio and whole life growth and fecundity. Related references: Gobler, C.J. and Baumann, H. (2016) Hypoxia and acidification in ocean ecosystems: Coupled dynamics and effects on marine life. Biology Letters 12:20150976. doi:10.1098/rsbl.2015.0976 Baumann, H. (2016) Combined effects of ocean acidification, warming, and hypoxia on marine organisms. Limnology and Oceanography e-Lectures 6:1-43. doi:10.1002/loe2.10002 Depasquale, E., Baumann, H., and Gobler, C.J. (2015) Variation in early life stage vulnerability among Northwest Atlantic estuarine forage fish to ocean acidification and low oxygen Marine Ecology Progress Series 523: 145–156.doi:10.3354/meps11142
attribute	NC_GLOBAL	projects_0_end_date	String	2018-11
attribute	NC_GLOBAL	projects_0_geolocation	String	Eastern Long Island Sound, CT, USA
attribute	NC_GLOBAL	projects_0_name	String	Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish
attribute	NC_GLOBAL	projects_0_project_nid	String	650184
attribute	NC_GLOBAL	projects_0_start_date	String	2015-12
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	41.32133889
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	subsetVariables	String	species,adult_collection_site,latitude,longitude,temp
attribute	NC_GLOBAL	summary	String	Total length, wet weight, and condition factor of juvenile Menidia\u00a0menidia reared at contrasting CO2 levels. These data are\u00a0associated with the corresponding paper: Murray, C.S., Fuiman, L., and Baumann, H.\u00a0Consequences of elevated CO2\u00a0exposure across multiple life stages in a coastal forage fish.\u00a0ICES Journal of Marine Science\u00a074:1051-1061(2017).
attribute	NC_GLOBAL	title	String	Laboratory study of long-term growth in juvenile Menidia menidia (Atlantic silverside) at contrasting CO2 levels for 16 to 122 days in 2015
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	Westernmost_Easting	double	-72.01483611
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	species		String
attribute	species	bcodmo_name	String	species
attribute	species	description	String	Menidia menidia; the Atlantic silverside
attribute	species	long_name	String	Species
attribute	species	units	String	unitless
variable	adult_collection_site		String
attribute	adult_collection_site	bcodmo_name	String	site
attribute	adult_collection_site	description	String	site of the collection of wild adult spawners on 1 May 2015
attribute	adult_collection_site	long_name	String	Adult Collection Site
attribute	adult_collection_site	units	String	unitless
variable	latitude		double
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	_FillValue	double	NaN
attribute	latitude	actual_range	double	41.32133889, 41.32133889
attribute	latitude	axis	String	Y
attribute	latitude	bcodmo_name	String	latitude
attribute	latitude	colorBarMaximum	double	90.0
attribute	latitude	colorBarMinimum	double	-90.0
attribute	latitude	description	String	latitude of the adult collection site
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LATX/
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	longitude		double
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	_FillValue	double	NaN
attribute	longitude	actual_range	double	-72.01483611, -72.01483611
attribute	longitude	axis	String	X
attribute	longitude	bcodmo_name	String	longitude
attribute	longitude	colorBarMaximum	double	180.0
attribute	longitude	colorBarMinimum	double	-180.0
attribute	longitude	description	String	longitude of the adult collections site
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LONX/
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east
variable	date_fertilization		String
attribute	date_fertilization	bcodmo_name	String	date
attribute	date_fertilization	description	String	date offspring were fertilized by strip-spawning; over twenty adults of each sex; format mm-dd-YYYY
attribute	date_fertilization	long_name	String	Date Fertilization
attribute	date_fertilization	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	date_fertilization	source_name	String	date_fertilization
attribute	date_fertilization	time_precision	String	1970-01-01
attribute	date_fertilization	units	String	unitless
variable	age		byte
attribute	age	_FillValue	byte	127
attribute	age	actual_range	byte	16, 122
attribute	age	bcodmo_name	String	age
attribute	age	description	String	age in days post hatch (dph) on the date of sampling
attribute	age	long_name	String	Age
attribute	age	units	String	days
variable	date_sample		String
attribute	date_sample	bcodmo_name	String	date
attribute	date_sample	description	String	date offspring were sampled for length and weight measurements; format mm-dd-YYYY
attribute	date_sample	long_name	String	Date Sample
attribute	date_sample	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	date_sample	time_precision	String	1970-01-01
attribute	date_sample	units	String	unitless
variable	temp		byte
attribute	temp	_FillValue	byte	127
attribute	temp	actual_range	byte	17, 17
attribute	temp	bcodmo_name	String	temperature
attribute	temp	description	String	seventeen degrees celcius; all fish experienced the same temperature
attribute	temp	long_name	String	Temperature
attribute	temp	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/
attribute	temp	units	String	degrees celsius
variable	pH_treatment		float
attribute	pH_treatment	_FillValue	float	NaN
attribute	pH_treatment	actual_range	float	7.45, 8.05
attribute	pH_treatment	bcodmo_name	String	pH
attribute	pH_treatment	description	String	average pH levels experienced by the fish; 7.45 or 8.05
attribute	pH_treatment	long_name	String	P H Treatment
attribute	pH_treatment	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/
attribute	pH_treatment	units	String	dimensionless
variable	CO2_treatment		short
attribute	CO2_treatment	_FillValue	short	32767
attribute	CO2_treatment	actual_range	short	500, 2150
attribute	CO2_treatment	bcodmo_name	String	pCO2
attribute	CO2_treatment	description	String	calculated pCO2 levels; calculated by CO2SYS; based on alkalinity pH and temperature
attribute	CO2_treatment	long_name	String	CO2 Treatment
attribute	CO2_treatment	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/
attribute	CO2_treatment	units	String	microatomosphere
variable	length_total		float
attribute	length_total	_FillValue	float	NaN
attribute	length_total	actual_range	float	6.15, 59.4
attribute	length_total	bcodmo_name	String	fish_len
attribute	length_total	description	String	total length of the fish at sampling
attribute	length_total	long_name	String	Length Total
attribute	length_total	units	String	millimeter
variable	wet_weight		short
attribute	wet_weight	_FillValue	short	32767
attribute	wet_weight	actual_range	short	30, 750
attribute	wet_weight	bcodmo_name	String	weight
attribute	wet_weight	description	String	wet weight of the fish at sampling
attribute	wet_weight	long_name	String	Wet Weight
attribute	wet_weight	units	String	milligram
variable	condition_factor		float
attribute	condition_factor	_FillValue	float	NaN
attribute	condition_factor	actual_range	float	0.3317, 0.9544
attribute	condition_factor	bcodmo_name	String	unknown
attribute	condition_factor	description	String	condition factor calculated as wetWeight/totalLength to the 2.734608 power
attribute	condition_factor	long_name	String	Condition Factor
attribute	condition_factor	units	String	dimensionless
variable	tank		String
attribute	tank	bcodmo_name	String	tank
attribute	tank	description	String	tank number; A B C D
attribute	tank	long_name	String	Tank
attribute	tank	units	String	unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.