ERDDAP > info > bcodmo_dataset_660050

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	Experimental data on growth rates of Pleurochrysis carterae analyzed at Bigelow Laboratory from 2013. (OA Copes Coccoliths project)		I   M	background			BCO-DMO	bcodmo_dataset_660050

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Cultures:\u00a0Pleurochrysis\u00a0carterae\u00a0cultures were maintained in exponential growth phase under axenic conditions in semi-continuous batch culture using L1-Si media prepared on 0.2 um-filtered, UV-sterilized, autoclaved seawater.\u00a0 Cultures were acclimated to one of three\u00a0pCO2\u00a0treatments for > 9 generations before experiments were performed.\u00a0 Cultures were maintained in an incubator at 16.5 +/- 0.5 degrees C and 470 umol photons/m-2/s\u00a0PAR on a 14-10 light-dark cycle. pCO2\u00a0Treatments: Carbonate chemistry was manipulated by bubbling cultures and prepared media with 500 mL/min\u00a0with 0.2 um-filtered 280, 380, or 750 ppm\u00a0pCO2\u00a0air.\u00a0 The\u00a0pCO2\u00a0levels of the treatment air were established using two mass flow controllers (Aalborg, Orangeburg, NY, USA) for each treatment to precisely mix in-house compressed air and pure CO2\u00a0(Maine Oxy, Auburn, ME, USA).\u00a0 The in-house compressed air was stripped of CO2\u00a0to less than 10 ppm CO2\u00a0using a Puregas VCD CO2\u00a0Adsorber (Puregas, LLC, Broomfield, CO, USA).\u00a0 The\u00a0pCO2\u00a0of the gas mixtures was stable to +/- 8 ppm.\u00a0\u00a0pCO2\u00a0values of the cultures may be different than the target levels due to biological activity. Growth rate measurements:\u00a0 At the same time each day, the cell density of each\u00a0pCO2\u00a0treatment culture was measured in order to calculate the growth rate.\u00a0 The data analyzed represent three consecutive growth cycles. Cell density:\u00a0Culture density was measured using a Moxi Z mini automated cell counter (ORFLO Technologies, Ketchum, ID, USA), which has a coefficient of variation of 4%.
attribute	NC_GLOBAL	awards_0_award_nid	String	514411
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1220068
attribute	NC_GLOBAL	awards_0_data_url	String	http://nsf.gov/awardsearch/showAward?AWD_ID=1220068
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Growth Rate of P. carterae W. Balch and D. Fields, PIs Version 28 September 2016
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2016-09-28T21:44:38Z
attribute	NC_GLOBAL	date_modified	String	2019-04-23T21:30:15Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.660050.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/660050
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	MFC
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Indicate and control set flow rates of gases. Manufactured in Orangeburg, NY USA.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	660059
attribute	NC_GLOBAL	instruments_0_description	String	Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Mass Flow Controller
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	712
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Aalborg Mass Flow Controller
attribute	NC_GLOBAL	instruments_1_acronym	String	CO2 Adsorber
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Instrument stripped compressed air of CO2
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	660060
attribute	NC_GLOBAL	instruments_1_description	String	CO2 Adsorber - an instrument designed to remove CO2 and moisture from compressed air.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	CO2 Adsorber
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	651526
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Puregas VCD CO2 Adsorber
attribute	NC_GLOBAL	instruments_2_acronym	String	ACC
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Measures culture density
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	660061
attribute	NC_GLOBAL	instruments_2_description	String	Automated Cell Counter (ACC) - a tool used for counting live and/or dead cells in a culture.  It can also be used to size particles.
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Automated Cell Counter
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	651528
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Moxi Z Automated Cell Counter
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, carbon, carbon dioxide, cell, cell_density, chemical, co2, cycle, data, dataset, day, density, deviation, dioxide, dmo, erddap, growth, growth_cycle, growthRate, intercept, linest, linest_intercept, linest_slope, ln_cellDensity, management, mean, mean_growthRate, oceanography, office, pCO2_treatment, preliminary, rate, slope, standard, standard deviation, stdev, stdev_growthRate, treatment
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/660050/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/660050
attribute	NC_GLOBAL	param_mapping	String	{'660050': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/660050/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Bigelow Laboratory for Ocean Sciences
attribute	NC_GLOBAL	people_0_person_name	String	William M. Balch
attribute	NC_GLOBAL	people_0_person_nid	String	50650
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Bigelow Laboratory for Ocean Sciences
attribute	NC_GLOBAL	people_1_person_name	String	David Fields
attribute	NC_GLOBAL	people_1_person_nid	String	51141
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Bigelow Laboratory for Ocean Sciences
attribute	NC_GLOBAL	people_2_person_name	String	William M. Balch
attribute	NC_GLOBAL	people_2_person_nid	String	50650
attribute	NC_GLOBAL	people_2_role	String	Contact
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	people_3_affiliation	String	Bigelow Laboratory for Ocean Sciences
attribute	NC_GLOBAL	people_3_person_name	String	Meredith White
attribute	NC_GLOBAL	people_3_person_nid	String	514420
attribute	NC_GLOBAL	people_3_role	String	Contact
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	people_4_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_4_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_4_person_name	String	Hannah Ake
attribute	NC_GLOBAL	people_4_person_nid	String	650173
attribute	NC_GLOBAL	people_4_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_4_role_type	String	related
attribute	NC_GLOBAL	project	String	OA_Copes_Coccoliths
attribute	NC_GLOBAL	projects_0_acronym	String	OA_Copes_Coccoliths
attribute	NC_GLOBAL	projects_0_description	String	(Extracted from the NSF award abstract) Ocean acidification is one of the most pressing marine science issues of our time, with potential biological impacts spanning all marine phyla and potential societal impacts affecting man's relationship to the sea. Rising levels of atmospheric pCO2 are increasing the acidity of the world oceans. It is generally held that average surface ocean pH has already declined by 0.1 pH units relative to the pre-industrial level (Orr et al., 2005), and is projected to decrease 0.3 to 0.46 units by the end of this century, depending on CO2 emission scenarios (Caldeira and Wickett, 2005). The overall goal of this research is to parameterize how changes in pCO2 levels could alter the biological and alkalinity pumps of the world ocean. Specifically, the direct and indirect effects of ocean acidification will be examined within a simple, controlled predator/prey system containing a single prey phytoplankton species (the coccolithophore, Emiliania huxleyi) and a single predator (the oceanic metazoan grazer, Calanus finmarchicus). The experiments are designed to elucidate both direct effects (i.e. effects of ocean acidification on the individual organisms only) and interactive effects (i.e. effects on the combined predator/prey system). Interactive experiments with phytoplankton prey and zooplankton predator are a critical starting point for predicting the overall impact of ocean acidification in marine ecosystems. To meet these goals, a state-of-the-art facility will be constructed with growth chambers that are calibrated and have highly-controlled pH and alkalinity levels. The strength of this approach lies in meticulous calibration and redundant measurements that will be made to ensure that conditions within the chambers are well described and tightly monitored for DIC levels. Growth and calcification rates in coccolithophores and the developmental rates, morphological and behavioral effects on copepods will be measured. The PIC and POC in the algae and the excreted fecal pellets will be monitored for changes in the PIC/POC ratio, a key parameter for modeling feedback mechanisms for rising pCO2 levels. In addition, 14C experiments are planned to measure calcification rates in coccolithophores and dissolution rates as a result of grazing. These key experiments will verify closure in the mass balance of PIC, allowing the determination of actual dissolution rates of PIC within the guts of copepod grazers.
attribute	NC_GLOBAL	projects_0_end_date	String	2015-07
attribute	NC_GLOBAL	projects_0_geolocation	String	Laboratory experiments; East Boothbay, Maine
attribute	NC_GLOBAL	projects_0_name	String	Effects of ocean acidification on Emiliania huxleyi and Calanus finmarchicus; insights into the oceanic alkalinity and biological carbon pumps
attribute	NC_GLOBAL	projects_0_project_nid	String	514415
attribute	NC_GLOBAL	projects_0_start_date	String	2012-08
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Experimental data on growth rates of Pleurochrysis carterae analyzed at Bigelow Laboratory from 2013. (OA Copes Coccoliths project)
attribute	NC_GLOBAL	title	String	Experimental data on growth rates of Pleurochrysis carterae analyzed at Bigelow Laboratory from 2013. (OA Copes Coccoliths project)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	pCO2_treatment		short
attribute	pCO2_treatment	_FillValue	short	32767
attribute	pCO2_treatment	actual_range	short	280, 750
attribute	pCO2_treatment	bcodmo_name	String	treatment
attribute	pCO2_treatment	description	String	The independent variable; one of three pCO2 levels (280 ppm, 380 ppm, or 750 ppm). These treatment levels are nominal values as they represent the target pCO2 for each treatment.
attribute	pCO2_treatment	long_name	String	P CO2 Treatment
attribute	pCO2_treatment	units	String	ppm
variable	day		float
attribute	day	_FillValue	float	NaN
attribute	day	actual_range	float	59.2, 71.0
attribute	day	bcodmo_name	String	day
attribute	day	description	String	The day the measurement was taken (since cultures were started).
attribute	day	long_name	String	Day
attribute	day	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/DAYXXXXX/
attribute	day	units	String	unitless
variable	growth_cycle		byte
attribute	growth_cycle	_FillValue	byte	127
attribute	growth_cycle	actual_range	byte	1, 3
attribute	growth_cycle	bcodmo_name	String	growth
attribute	growth_cycle	description	String	These data come from three consecutive growth cycles.
attribute	growth_cycle	long_name	String	Growth Cycle
attribute	growth_cycle	units	String	unitless
variable	cell_density		int
attribute	cell_density	_FillValue	int	2147483647
attribute	cell_density	actual_range	int	1900, 222000
attribute	cell_density	bcodmo_name	String	cell_concentration
attribute	cell_density	description	String	Cell density of the culture as measured by the Moxi Z Automated Cell Counter.
attribute	cell_density	long_name	String	Cell Density
attribute	cell_density	units	String	cell per milliliter cells/mL
variable	ln_cellDensity		float
attribute	ln_cellDensity	_FillValue	float	NaN
attribute	ln_cellDensity	actual_range	float	7.55, 12.31
attribute	ln_cellDensity	bcodmo_name	String	cell_concentration
attribute	ln_cellDensity	description	String	The natural log of the cell density.
attribute	ln_cellDensity	long_name	String	Ln Cell Density
attribute	ln_cellDensity	units	String	ln(cells/mL)
variable	linest_slope		float
attribute	linest_slope	_FillValue	float	NaN
attribute	linest_slope	actual_range	float	0.374, 0.751
attribute	linest_slope	bcodmo_name	String	growth
attribute	linest_slope	description	String	The slope resulting from the excel function LINEST which calculates the statistics for a line by using the 'least squares' method to calculate a straight line that best fits the data. One line is calculated for each growth cycle and the slope represents the growth rate of the algae during that growth cycle. In this case the line was calculated from ln(cell density) and day.
attribute	linest_slope	long_name	String	Linest Slope
attribute	linest_slope	units	String	(ln(cells/mL))/day
variable	linest_intercept		float
attribute	linest_intercept	_FillValue	float	NaN
attribute	linest_intercept	actual_range	float	-37.12, -15.6
attribute	linest_intercept	bcodmo_name	String	unknown
attribute	linest_intercept	description	String	The intercept resulting from the excel function LINEST which calculates the statistics for a line by using the 'least squares' method to calculate a straight line that best fits the data. One line is calculated for each growth cycle.
attribute	linest_intercept	long_name	String	Linest Intercept
attribute	linest_intercept	units	String	unitless
variable	growthRate		float
attribute	growthRate	_FillValue	float	NaN
attribute	growthRate	actual_range	float	0.374, 0.751
attribute	growthRate	bcodmo_name	String	growth
attribute	growthRate	description	String	The growth rate for the given growth cycle. This is the slope of the line fit to the ln(cell density) and day data. One growth rate is calculated for each growth cycle.
attribute	growthRate	long_name	String	Growth Rate
attribute	growthRate	units	String	u/day
variable	mean_growthRate		float
attribute	mean_growthRate	_FillValue	float	NaN
attribute	mean_growthRate	actual_range	float	0.46, 0.58
attribute	mean_growthRate	bcodmo_name	String	growth
attribute	mean_growthRate	description	String	The average growth rate from the three growth cycles.
attribute	mean_growthRate	long_name	String	Mean Growth Rate
attribute	mean_growthRate	units	String	u/day
variable	stdev_growthRate		float
attribute	stdev_growthRate	_FillValue	float	NaN
attribute	stdev_growthRate	actual_range	float	0.1, 0.16
attribute	stdev_growthRate	bcodmo_name	String	standard deviation
attribute	stdev_growthRate	description	String	The standard deviation of the growth rates from the three growth cycles.
attribute	stdev_growthRate	long_name	String	Stdev Growth Rate
attribute	stdev_growthRate	units	String	day-1

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.