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     data   graph     files  public Experimental data on growth rates of Pleurochrysis carterae analyzed at Bigelow Laboratory
from 2013. (OA Copes Coccoliths project)
   ?     I   M   background (external link) RSS BCO-DMO bcodmo_dataset_660050

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Cultures:\u00a0Pleurochrysis\u00a0carterae\u00a0cultures were maintained in
exponential growth phase under axenic conditions in semi-continuous batch
culture using L1-Si media prepared on 0.2 um-filtered, UV-sterilized,
autoclaved seawater.\u00a0 Cultures were acclimated to one of
three\u00a0pCO2\u00a0treatments for > 9 generations before experiments were
performed.\u00a0 Cultures were maintained in an incubator at 16.5 +/- 0.5
degrees C and 470 umol photons/m-2/s\u00a0PAR on a 14-10 light-dark cycle.

pCO2\u00a0Treatments: Carbonate chemistry was manipulated by bubbling cultures
and prepared media with 500 mL/min\u00a0with 0.2 um-filtered 280, 380, or 750
ppm\u00a0pCO2\u00a0air.\u00a0 The\u00a0pCO2\u00a0levels of the treatment air
were established using two mass flow controllers (Aalborg, Orangeburg, NY,
USA) for each treatment to precisely mix in-house compressed air and pure
CO2\u00a0(Maine Oxy, Auburn, ME, USA).\u00a0 The in-house compressed air was
stripped of CO2\u00a0to less than 10 ppm CO2\u00a0using a Puregas VCD
CO2\u00a0Adsorber (Puregas, LLC, Broomfield, CO, USA).\u00a0
The\u00a0pCO2\u00a0of the gas mixtures was stable to +/- 8
ppm.\u00a0\u00a0pCO2\u00a0values of the cultures may be different than the
target levels due to biological activity.

Growth rate measurements:\u00a0 At the same time each day, the cell density of
each\u00a0pCO2\u00a0treatment culture was measured in order to calculate the
growth rate.\u00a0 The data analyzed represent three consecutive growth
cycles.

Cell density:\u00a0Culture density was measured using a Moxi Z mini automated
cell counter (ORFLO Technologies, Ketchum, ID, USA), which has a coefficient
of variation of 4%.
attribute NC_GLOBAL awards_0_award_nid String 514411
attribute NC_GLOBAL awards_0_award_number String OCE-1220068
attribute NC_GLOBAL awards_0_data_url String http://nsf.gov/awardsearch/showAward?AWD_ID=1220068 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Dr David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Growth Rate of P. carterae
W. Balch and D. Fields, PIs
Version 28 September 2016
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv.pl v1.0
attribute NC_GLOBAL date_created String 2016-09-28T21:44:38Z
attribute NC_GLOBAL date_modified String 2019-04-23T21:30:15Z
attribute NC_GLOBAL defaultDataQuery String &time
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.660050.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/660050 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String MFC
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Indicate and control set flow rates of gases. Manufactured in Orangeburg, NY USA.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 660059
attribute NC_GLOBAL instruments_0_description String Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases
attribute NC_GLOBAL instruments_0_instrument_name String Mass Flow Controller
attribute NC_GLOBAL instruments_0_instrument_nid String 712
attribute NC_GLOBAL instruments_0_supplied_name String Aalborg Mass Flow Controller
attribute NC_GLOBAL instruments_1_acronym String CO2 Adsorber
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Instrument stripped compressed air of CO2
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 660060
attribute NC_GLOBAL instruments_1_description String CO2 Adsorber -�an instrument designed to�remove CO2 and moisture from compressed air.
attribute NC_GLOBAL instruments_1_instrument_name String CO2 Adsorber
attribute NC_GLOBAL instruments_1_instrument_nid String 651526
attribute NC_GLOBAL instruments_1_supplied_name String Puregas VCD CO2 Adsorber
attribute NC_GLOBAL instruments_2_acronym String ACC
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Measures culture density
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 660061
attribute NC_GLOBAL instruments_2_description String Automated Cell Counter (ACC) - a tool used for�counting live and/or dead cells in a culture.��It can also be used to size particles.
attribute NC_GLOBAL instruments_2_instrument_name String Automated Cell Counter
attribute NC_GLOBAL instruments_2_instrument_nid String 651528
attribute NC_GLOBAL instruments_2_supplied_name String Moxi Z Automated Cell Counter
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, carbon, carbon dioxide, cell, cell_density, chemical, co2, cycle, data, dataset, day, density, deviation, dioxide, dmo, erddap, growth, growth_cycle, growthRate, intercept, linest, linest_intercept, linest_slope, ln_cellDensity, management, mean, mean_growthRate, oceanography, office, pCO2_treatment, physical, physical oceanography, preliminary, rate, slope, standard, standard deviation, statistics, stdev, stdev_growthRate, time, treatment
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/660050 (external link)
attribute NC_GLOBAL param_mapping String {'660050': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/660050/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Bigelow Laboratory for Ocean Sciences
attribute NC_GLOBAL people_0_person_name String William M. Balch
attribute NC_GLOBAL people_0_person_nid String 50650
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Bigelow Laboratory for Ocean Sciences
attribute NC_GLOBAL people_1_person_name String Dr David Fields
attribute NC_GLOBAL people_1_person_nid String 51141
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Bigelow Laboratory for Ocean Sciences
attribute NC_GLOBAL people_2_person_name String William M. Balch
attribute NC_GLOBAL people_2_person_nid String 50650
attribute NC_GLOBAL people_2_role String Contact
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL people_3_affiliation String Bigelow Laboratory for Ocean Sciences
attribute NC_GLOBAL people_3_person_name String Dr Meredith White
attribute NC_GLOBAL people_3_person_nid String 514420
attribute NC_GLOBAL people_3_role String Contact
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL people_4_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_4_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_4_person_name String Hannah Ake
attribute NC_GLOBAL people_4_person_nid String 650173
attribute NC_GLOBAL people_4_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_4_role_type String related
attribute NC_GLOBAL project String Effects of ocean acidification on Emiliania huxleyi and Calanus finmarchicus; insights into the oceanic alkalinity and biological carbon pumps
attribute NC_GLOBAL projects_0_acronym String OA_Copes_Coccoliths
attribute NC_GLOBAL projects_0_description String (Extracted from the NSF award abstract)
Ocean acidification is one of the most pressing marine science issues of our time, with potential biological impacts spanning all marine phyla and potential societal impacts affecting man's relationship to the sea. Rising levels of atmospheric pCO2 are increasing the acidity of the world oceans. It is generally held that average surface ocean pH has already declined by 0.1 pH units relative to the pre-industrial level (Orr et al., 2005), and is projected to decrease 0.3 to 0.46 units by the end of this century, depending on CO2 emission scenarios (Caldeira and Wickett, 2005). The overall goal of this research is to parameterize how changes in pCO2 levels could alter the biological and alkalinity pumps of the world ocean. Specifically, the direct and indirect effects of ocean acidification will be examined within a simple, controlled predator/prey system containing a single prey phytoplankton species (the coccolithophore, Emiliania huxleyi) and a single predator (the oceanic metazoan grazer, Calanus finmarchicus). The experiments are designed to elucidate both direct effects (i.e. effects of ocean acidification on the individual organisms only) and interactive effects (i.e. effects on the combined predator/prey system). Interactive experiments with phytoplankton prey and zooplankton predator are a critical starting point for predicting the overall impact of ocean acidification in marine ecosystems. To meet these goals, a state-of-the-art facility will be constructed with growth chambers that are calibrated and have highly-controlled pH and alkalinity levels. The strength of this approach lies in meticulous calibration and redundant measurements that will be made to ensure that conditions within the chambers are well described and tightly monitored for DIC levels. Growth and calcification rates in coccolithophores and the developmental rates, morphological and behavioral effects on copepods will be measured. The PIC and POC in the algae and the excreted fecal pellets will be monitored for changes in the PIC/POC ratio, a key parameter for modeling feedback mechanisms for rising pCO2 levels. In addition, 14C experiments are planned to measure calcification rates in coccolithophores and dissolution rates as a result of grazing. These key experiments will verify closure in the mass balance of PIC, allowing the determination of actual dissolution rates of PIC within the guts of copepod grazers.
attribute NC_GLOBAL projects_0_end_date String 2015-07
attribute NC_GLOBAL projects_0_geolocation String Laboratory experiments; East Boothbay, Maine
attribute NC_GLOBAL projects_0_name String Effects of ocean acidification on Emiliania huxleyi and Calanus finmarchicus; insights into the oceanic alkalinity and biological carbon pumps
attribute NC_GLOBAL projects_0_project_nid String 514415
attribute NC_GLOBAL projects_0_start_date String 2012-08
attribute NC_GLOBAL publisher_name String Hannah Ake
attribute NC_GLOBAL publisher_role String BCO-DMO Data Manager(s)
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v29
attribute NC_GLOBAL summary String Experimental data on growth rates of Pleurochrysis carterae analyzed at Bigelow Laboratory from 2013. (OA Copes Coccoliths project)
attribute NC_GLOBAL title String Experimental data on growth rates of Pleurochrysis carterae analyzed at Bigelow Laboratory from 2013. (OA Copes Coccoliths project)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.0-alpha
variable pCO2_treatment   short  
attribute pCO2_treatment _FillValue short 32767
attribute pCO2_treatment actual_range short 280, 750
attribute pCO2_treatment description String The independent variable; one of three pCO2 levels (280 ppm, 380 ppm, or 750 ppm). These treatment levels are nominal values as they represent the target pCO2 for each treatment.
attribute pCO2_treatment ioos_category String CO2
attribute pCO2_treatment long_name String P CO2 Treatment
attribute pCO2_treatment units String ppm
variable day   float  
attribute day _FillValue float NaN
attribute day actual_range float 59.2, 71.0
attribute day description String The day the measurement was taken (since cultures were started).
attribute day ioos_category String Time
attribute day long_name String Day
attribute day units String unitless
variable growth_cycle   byte  
attribute growth_cycle _FillValue byte 127
attribute growth_cycle actual_range byte 1, 3
attribute growth_cycle description String These data come from three consecutive growth cycles.
attribute growth_cycle ioos_category String Unknown
attribute growth_cycle long_name String Growth Cycle
attribute growth_cycle units String unitless
variable cell_density   int  
attribute cell_density _FillValue int 2147483647
attribute cell_density actual_range int 1900, 222000
attribute cell_density description String Cell density of the culture as measured by the Moxi Z Automated Cell Counter.
attribute cell_density ioos_category String Physical Oceanography
attribute cell_density long_name String Cell Density
attribute cell_density units String cell per milliliter cells/mL
variable ln_cellDensity   float  
attribute ln_cellDensity _FillValue float NaN
attribute ln_cellDensity actual_range float 7.55, 12.31
attribute ln_cellDensity description String The natural log of the cell density.
attribute ln_cellDensity ioos_category String Physical Oceanography
attribute ln_cellDensity long_name String Ln Cell Density
attribute ln_cellDensity units String ln(cells/mL)
variable linest_slope   float  
attribute linest_slope _FillValue float NaN
attribute linest_slope actual_range float 0.374, 0.751
attribute linest_slope description String The slope resulting from the excel function LINEST which calculates the statistics for a line by using the 'least squares' method to calculate a straight line that best fits the data. One line is calculated for each growth cycle and the slope represents the growth rate of the algae during that growth cycle. In this case the line was calculated from ln(cell density) and day.
attribute linest_slope ioos_category String Unknown
attribute linest_slope long_name String Linest Slope
attribute linest_slope units String (ln(cells/mL))/day
variable linest_intercept   float  
attribute linest_intercept _FillValue float NaN
attribute linest_intercept actual_range float -37.12, -15.6
attribute linest_intercept description String The intercept resulting from the excel function LINEST which calculates the statistics for a line by using the 'least squares' method to calculate a straight line that best fits the data. One line is calculated for each growth cycle.
attribute linest_intercept ioos_category String Unknown
attribute linest_intercept long_name String Linest Intercept
attribute linest_intercept units String unitless
variable growthRate   float  
attribute growthRate _FillValue float NaN
attribute growthRate actual_range float 0.374, 0.751
attribute growthRate description String The growth rate for the given growth cycle. This is the slope of the line fit to the ln(cell density) and day data. One growth rate is calculated for each growth cycle.
attribute growthRate ioos_category String Unknown
attribute growthRate long_name String Growth Rate
attribute growthRate units String u/day
variable mean_growthRate   float  
attribute mean_growthRate _FillValue float NaN
attribute mean_growthRate actual_range float 0.46, 0.58
attribute mean_growthRate description String The average growth rate from the three growth cycles.
attribute mean_growthRate ioos_category String Statistics
attribute mean_growthRate long_name String Mean Growth Rate
attribute mean_growthRate units String u/day
variable stdev_growthRate   float  
attribute stdev_growthRate _FillValue float NaN
attribute stdev_growthRate actual_range float 0.1, 0.16
attribute stdev_growthRate description String The standard deviation of the growth rates from the three growth cycles.
attribute stdev_growthRate ioos_category String Unknown
attribute stdev_growthRate long_name String Stdev Growth Rate
attribute stdev_growthRate units String day-1

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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