ERDDAP > info > bcodmo_dataset_660194

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Pleurochrysis carterae 24 hour culture dynamics] - Pleurochrysis carterae diel culture dynamics analyzed at Bigelow Laboratory from 2013 (OA Copes Coccoliths project) (Effects of ocean acidification on Emiliania huxleyi and Calanus finmarchicus; insights into the oceanic alkalinity and biological carbon pumps)		I   M	background			BCO-DMO	bcodmo_dataset_660194

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Cultures:\u00a0Pleurochrysis\u00a0carterae\u00a0cultures were maintained in exponential growth phase under axenic conditions in semi-continuous batch culture using L1-Si media prepared on 0.2 um-filtered, UV-sterilized, autoclaved seawater.\u00a0 Cultures were acclimated to one of three\u00a0pCO2\u00a0treatments for > 9 generations before experiments were performed.\u00a0 Cultures were maintained in an incubator at 16.5 +/- 0.5 \u00a0degrees C and 470 umol photons/m-2/s\u00a0PAR on a 14-10 light-dark cycle where the lights turned on at 6 am and turned off at 8 pm. pCO2\u00a0Treatments: Carbonate chemistry was manipulated by bubbling cultures and prepared media with 500 mL/min\u00a0with 0.2 um-filtered 280, 380, or 750 ppm\u00a0pCO2\u00a0air.\u00a0 The\u00a0pCO2\u00a0levels of the treatment air were established using two mass flow controllers (Aalborg, Orangeburg, NY, USA) for each treatment to precisely mix in-house compressed air and pure CO2\u00a0(Maine Oxy, Auburn, ME, USA).\u00a0 The in-house compressed air was stripped of CO2\u00a0to less than 10 ppm CO2\u00a0using a Puregas VCD CO2\u00a0Adsorber (Puregas, LLC, Broomfield, CO, USA).\u00a0 The\u00a0pCO2\u00a0of the gas mixtures was stable to +/- 8 ppm.\u00a0\u00a0pCO2\u00a0values of the cultures may be different than the target levels due to biological activity. 24 h Culture Dynamics Monitoring:\u00a0 To understand the chemical and biological culture dynamics over a 24 h period, the investigators took measurements of one culture from each\u00a0pCO2\u00a0treatment every hour for 24 h.\u00a0 To reduce analytical costs, some parameters were not measured each hour (PIC, nutrients, total alkalinity).\u00a0 During the dark period, a red lamp was used to limit the light that might influence the cultures. \u00a0 pH Measurements:\u00a0 The pH of the cultures was measured using an OrionTM\u00a0ROSSTM\u00a0electrode connected to an Orion StarTM\u00a0A211 Benchtop pH meter (ThermoFisher Scientific, Waltham, MA, USA), calibrated with NBS buffers (EK Industries, Inc., Joliet, IL, USA) and corrected to the total scale using spectrophotometric pH measurements of culture samples.\u00a0 Spectrophotometric pH measurements of 0.2 um-filtered culture samples were made with 20 mM\u00a0m-Cresol purple sodium salt indicator dye (Alfa Aesar, Ward Hill, MA, USA) using a Hitachi U-3010 spectrophotometer (Hitachi High- Technologies, Clarksburg, MD, USA) equipped with a water circulated cell holder connected to a VWR 1160 water bath (VWR, Radnor, PA, USA) set at 16.5 degrees C, holding a 1 cm quartz cell.\u00a0 The method followed the procedure described by Clayton and Byrne (1993) and Dickson et al. (2007), using the refit equation of Liu et al. (2011), resulting in a resolution of +/-0.004 pH units. \u00a0 Temperature:\u00a0Temperature measurements were made with an OrionTM\u00a0ROSSTM\u00a0electrode connected to an Orion StarTM\u00a0A211 Benchtop pH meter (ThermoFisher Scientific, Waltham, MA, USA). Salinity:\u00a0 Salinity was measured using an Acorn SALT 6 handheld salinity meter (Oakton Instruments, Vernon Hills, IL, USA) with a resolution of +/- 0.1 ppt. in vivo Fluorescence:\u00a0 Fluoresence was measured using a Turner 10-AU fluorometer (Turner Designs, Sunnyvale, CA, USA). Cell density and cell diameter: Culture density and mean cell diameter were measured using a Moxi Z mini automated cell counter (ORFLO Technologies, Ketchum, ID, USA), which has a coefficient of variation of 4%. Particulate Inorganic Carbon:\u00a0 Bulk culture PIC analyses followed the technique of Fernandez et al. (1993): 10 mL culture samples were filtered onto 0.4 um polycarbonate filters and rinsed with potassium borate buffer with the pH adjusted to 8.0 to remove seawater calcium chloride.\u00a0 Filters were carefully moved to trace-metal free centrifuge tubes and digested with 5 mL of 5% nitric acid.\u00a0 The calcium concentration was measured using a Jobin Yvon Ultima C inductively coupled plasma-atomic emission spectrometer (ICP- AES, HORIBA, Ltd., Kyoto, Japan).\u00a0 Bulk culture PIC measurements were corrected to PIC/cell-1\u00a0using the corresponding cell density measurements. Nutrients (measured at University of California Santa Barbara):\u00a0 Culture samples were filtered to 0.2 um to remove all algal cells and coccoliths, and samples were frozen prior to analysis.\u00a0 Total N (nitrate + nitrite), nitrate, phosphate, and silicate were measured by Flow Injection Analysis at the University of California, Santa Barbara, Marine Science Institute\u2019s Analytical Lab using a QuikChem 8000 (Lachat Instruments, Loveland, CO, USA). Nutrients (measured by Bigelow Analytical Services):\u00a0 Culture samples were filtered to 0.2 um to remove all algal cells and coccoliths, and samples were frozen prior to analysis.\u00a0 Nitrate and phosphate were measured by Continuous Flow Analysis by Bigelow Analytical Services using a SEAL AutoAnalyzer 3 HR (SEAL Analytical Inc., Mequon, WI, USA). Total Alkalinity:\u00a0 Culture samples were filtered to 0.2 um to remove all algal cells and coccoliths.\u00a0 Total alkalinity was measured via titration with 0.01 N HCl using a Metrohm Titrando 888 controlled by Tiamo software (Metrohm, Riverview, FL, USA) to perform automated Gran titrations of 4 mL samples.\u00a0 Titrations were corrected to Certified Reference Materials (supplied by the laboratory of Andrew Dickson, Scripps Institution of Oceanography, La Jolla, CA, USA).
attribute	NC_GLOBAL	awards_0_award_nid	String	514411
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1220068
attribute	NC_GLOBAL	awards_0_data_url	String	http://nsf.gov/awardsearch/showAward?AWD_ID=1220068
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	24 Hour Culture Dynamics W. Balch and D. Fields, PIs Version 28 September 2016
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2016-09-29T19:04:44Z
attribute	NC_GLOBAL	date_modified	String	2019-06-11T15:43:42Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.660194.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/660194
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Nutrient Autoanalyzer
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Continuous flow analysis performed by Bigelow Analytical Services (SEAL Analytical Inc., Mequon, WI, USA).
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	663847
attribute	NC_GLOBAL	instruments_0_description	String	Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB04/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Nutrient Autoanalyzer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	558
attribute	NC_GLOBAL	instruments_0_supplied_name	String	SEAL AutoAnalyzer 3 HR
attribute	NC_GLOBAL	instruments_1_acronym	String	pH Sensor
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Orion ROSS electrode was connected to an Orion Star A211 Benchtop pH meter (ThermoFisher Scientific, Waltham, MA, USA)
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	660206
attribute	NC_GLOBAL	instruments_1_description	String	General term for an instrument that measures the pH or how acidic or basic a solution is.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	pH Sensor
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	674
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Orion ROSS electrode
attribute	NC_GLOBAL	instruments_2_acronym	String	Automatic titrator
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Automatic titrations controlled by Tiamo software (Metrohm, Riverview, FL, USA).
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	660211
attribute	NC_GLOBAL	instruments_2_description	String	Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.
attribute	NC_GLOBAL	instruments_2_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB12/
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Automatic titrator
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	682
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Metrohm Titrando 888
attribute	NC_GLOBAL	instruments_3_acronym	String	Spectrophotometer
attribute	NC_GLOBAL	instruments_3_dataset_instrument_description	String	Spectrophotometric pH measurements were taken of culture samples
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	660207
attribute	NC_GLOBAL	instruments_3_description	String	An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.
attribute	NC_GLOBAL	instruments_3_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB20/
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Spectrophotometer
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	707
attribute	NC_GLOBAL	instruments_3_supplied_name	String	Hitachi U-3010 spectrophotometer
attribute	NC_GLOBAL	instruments_4_acronym	String	Salinity Sensor
attribute	NC_GLOBAL	instruments_4_dataset_instrument_description	String	Salinity measured using this instrument with a resolution of +/- 0.1 ppt (Oakton Instruments, Vernon Hills, IL, USA)
attribute	NC_GLOBAL	instruments_4_dataset_instrument_nid	String	660208
attribute	NC_GLOBAL	instruments_4_description	String	Category of instrument that simultaneously measures electrical conductivity and temperature in the water column to provide temperature and salinity data.
attribute	NC_GLOBAL	instruments_4_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/350/
attribute	NC_GLOBAL	instruments_4_instrument_name	String	Salinity Sensor
attribute	NC_GLOBAL	instruments_4_instrument_nid	String	710
attribute	NC_GLOBAL	instruments_4_supplied_name	String	Acorn SALT 6 handheld salinity meter
attribute	NC_GLOBAL	instruments_5_acronym	String	MFC
attribute	NC_GLOBAL	instruments_5_dataset_instrument_description	String	Indicate and control set flow rates of gases. Manufactured in Orangeburg, NY USA.
attribute	NC_GLOBAL	instruments_5_dataset_instrument_nid	String	660203
attribute	NC_GLOBAL	instruments_5_description	String	Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases
attribute	NC_GLOBAL	instruments_5_instrument_name	String	Mass Flow Controller
attribute	NC_GLOBAL	instruments_5_instrument_nid	String	712
attribute	NC_GLOBAL	instruments_5_supplied_name	String	Aalborg Mass Flow Controller
attribute	NC_GLOBAL	instruments_6_acronym	String	ICP-OES
attribute	NC_GLOBAL	instruments_6_dataset_instrument_description	String	Calcium concentration measured (ICP-AES, HORIBA, Ltd., Kyoto, Japan).
attribute	NC_GLOBAL	instruments_6_dataset_instrument_nid	String	660209
attribute	NC_GLOBAL	instruments_6_description	String	Also referred to as an Inductively coupled plasma atomic emission spectroscope (ICP-AES). These instruments pass nebulised samples into an inductively-coupled gas plasma (8-10000 K) where they are atomised and excited. The de-excitation optical emissions at characteristic wavelengths are spectroscopically analysed. It is often used in the detection of trace metals.
attribute	NC_GLOBAL	instruments_6_instrument_name	String	Inductively Coupled Plasma Optical Emission Spectrometer
attribute	NC_GLOBAL	instruments_6_instrument_nid	String	639924
attribute	NC_GLOBAL	instruments_6_supplied_name	String	Jobin Yvon Ultima C
attribute	NC_GLOBAL	instruments_7_acronym	String	CO2 Adsorber
attribute	NC_GLOBAL	instruments_7_dataset_instrument_description	String	Instrument stripped compressed air of CO2
attribute	NC_GLOBAL	instruments_7_dataset_instrument_nid	String	660204
attribute	NC_GLOBAL	instruments_7_description	String	CO2 Adsorber - an instrument designed to remove CO2 and moisture from compressed air.
attribute	NC_GLOBAL	instruments_7_instrument_name	String	CO2 Adsorber
attribute	NC_GLOBAL	instruments_7_instrument_nid	String	651526
attribute	NC_GLOBAL	instruments_7_supplied_name	String	Puregas VCD CO2 Adsorber
attribute	NC_GLOBAL	instruments_8_acronym	String	ACC
attribute	NC_GLOBAL	instruments_8_dataset_instrument_description	String	Measures culture density
attribute	NC_GLOBAL	instruments_8_dataset_instrument_nid	String	660205
attribute	NC_GLOBAL	instruments_8_description	String	Automated Cell Counter (ACC) - a tool used for counting live and/or dead cells in a culture.  It can also be used to size particles.
attribute	NC_GLOBAL	instruments_8_instrument_name	String	Automated Cell Counter
attribute	NC_GLOBAL	instruments_8_instrument_nid	String	651528
attribute	NC_GLOBAL	instruments_8_supplied_name	String	Moxi Z Automated Cell Counter
attribute	NC_GLOBAL	keywords	String	approx, approx_time, bco, bco-dmo, biological, calcite, carbon, carbon dioxide, carbonate, cell, cell_density, chemical, chemistry, co2, co3, concentration, cper, data, dataset, date, density, diameter, dic, dioxide, dmo, earth, Earth Science > Oceans > Ocean Chemistry > Nitrate, Earth Science > Oceans > Ocean Chemistry > pH, Earth Science > Oceans > Ocean Chemistry > Phosphate, Earth Science > Oceans > Salinity/Density > Salinity, elapsed, elapsed_time, erddap, fluorescence, fluorescencePerCellDensity, hco3, inVivo_fluorescence, iso, light, management, mass, mass_concentration_of_phosphate_in_sea_water, mean, mean_cellDiameter, mean_SiO4, mole, mole_concentration_of_nitrate_in_sea_water, n02, nitrate, no3, NO3_Bigelow, NO3_NO2, NO3_UCSB, ocean, oceanography, oceans, office, omega, omega_calcite, pCO2, pCO2_treatment, per, phosphate, pic, PIC_pgCPerCell, PIC_ugCPerL, po4, PO4_Bigelow, PO4_UCSB, practical, preliminary, reported, salinity, scale, science, sea, sea_water_ph_reported_on_total_scale, sea_water_practical_salinity, seawater, SiO4, temperature, time, time2, total, treatment, vivo, water
attribute	NC_GLOBAL	keywords_vocabulary	String	GCMD Science Keywords
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/660194/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/660194
attribute	NC_GLOBAL	param_mapping	String	{'660194': {'ISO_DateTime_UTC': 'flag - time'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/660194/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Bigelow Laboratory for Ocean Sciences
attribute	NC_GLOBAL	people_0_person_name	String	William M. Balch
attribute	NC_GLOBAL	people_0_person_nid	String	50650
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Bigelow Laboratory for Ocean Sciences
attribute	NC_GLOBAL	people_1_person_name	String	David Fields
attribute	NC_GLOBAL	people_1_person_nid	String	51141
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Bigelow Laboratory for Ocean Sciences
attribute	NC_GLOBAL	people_2_person_name	String	William M. Balch
attribute	NC_GLOBAL	people_2_person_nid	String	50650
attribute	NC_GLOBAL	people_2_role	String	Contact
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	people_3_affiliation	String	Bigelow Laboratory for Ocean Sciences
attribute	NC_GLOBAL	people_3_person_name	String	Meredith White
attribute	NC_GLOBAL	people_3_person_nid	String	514420
attribute	NC_GLOBAL	people_3_role	String	Contact
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	people_4_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_4_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_4_person_name	String	Hannah Ake
attribute	NC_GLOBAL	people_4_person_nid	String	650173
attribute	NC_GLOBAL	people_4_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_4_role_type	String	related
attribute	NC_GLOBAL	project	String	OA_Copes_Coccoliths
attribute	NC_GLOBAL	projects_0_acronym	String	OA_Copes_Coccoliths
attribute	NC_GLOBAL	projects_0_description	String	(Extracted from the NSF award abstract) Ocean acidification is one of the most pressing marine science issues of our time, with potential biological impacts spanning all marine phyla and potential societal impacts affecting man's relationship to the sea. Rising levels of atmospheric pCO2 are increasing the acidity of the world oceans. It is generally held that average surface ocean pH has already declined by 0.1 pH units relative to the pre-industrial level (Orr et al., 2005), and is projected to decrease 0.3 to 0.46 units by the end of this century, depending on CO2 emission scenarios (Caldeira and Wickett, 2005). The overall goal of this research is to parameterize how changes in pCO2 levels could alter the biological and alkalinity pumps of the world ocean. Specifically, the direct and indirect effects of ocean acidification will be examined within a simple, controlled predator/prey system containing a single prey phytoplankton species (the coccolithophore, Emiliania huxleyi) and a single predator (the oceanic metazoan grazer, Calanus finmarchicus). The experiments are designed to elucidate both direct effects (i.e. effects of ocean acidification on the individual organisms only) and interactive effects (i.e. effects on the combined predator/prey system). Interactive experiments with phytoplankton prey and zooplankton predator are a critical starting point for predicting the overall impact of ocean acidification in marine ecosystems. To meet these goals, a state-of-the-art facility will be constructed with growth chambers that are calibrated and have highly-controlled pH and alkalinity levels. The strength of this approach lies in meticulous calibration and redundant measurements that will be made to ensure that conditions within the chambers are well described and tightly monitored for DIC levels. Growth and calcification rates in coccolithophores and the developmental rates, morphological and behavioral effects on copepods will be measured. The PIC and POC in the algae and the excreted fecal pellets will be monitored for changes in the PIC/POC ratio, a key parameter for modeling feedback mechanisms for rising pCO2 levels. In addition, 14C experiments are planned to measure calcification rates in coccolithophores and dissolution rates as a result of grazing. These key experiments will verify closure in the mass balance of PIC, allowing the determination of actual dissolution rates of PIC within the guts of copepod grazers.
attribute	NC_GLOBAL	projects_0_end_date	String	2015-07
attribute	NC_GLOBAL	projects_0_geolocation	String	Laboratory experiments; East Boothbay, Maine
attribute	NC_GLOBAL	projects_0_name	String	Effects of ocean acidification on Emiliania huxleyi and Calanus finmarchicus; insights into the oceanic alkalinity and biological carbon pumps
attribute	NC_GLOBAL	projects_0_project_nid	String	514415
attribute	NC_GLOBAL	projects_0_start_date	String	2012-08
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Pleurochrysis carterae diel culture dynamics analyzed at Bigelow Laboratory from 2013 (OA Copes Coccoliths project)
attribute	NC_GLOBAL	time_coverage_end	String	2013-07-18T10:23:00Z
attribute	NC_GLOBAL	time_coverage_start	String	2013-07-17T09:05:00Z
attribute	NC_GLOBAL	title	String	[Pleurochrysis carterae 24 hour culture dynamics] - Pleurochrysis carterae diel culture dynamics analyzed at Bigelow Laboratory from 2013 (OA Copes Coccoliths project) (Effects of ocean acidification on Emiliania huxleyi and Calanus finmarchicus; insights into the oceanic alkalinity and biological carbon pumps)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	pCO2_treatment		short
attribute	pCO2_treatment	_FillValue	short	32767
attribute	pCO2_treatment	actual_range	short	280, 750
attribute	pCO2_treatment	bcodmo_name	String	treatment
attribute	pCO2_treatment	description	String	The independent variable; one of three pCO2 levels (280 ppm, 380 ppm, or 750 ppm). These treatment levels are nominal values as they represent the target pCO2 for each treatment. Within each pCO2 treatment there are 14 days worth of culture measurements for the algal cultures. Measurements of the media (without algae) were made on days 0 and 14. Only chemical measurements were made on the media not biological measurements.
attribute	pCO2_treatment	long_name	String	P CO2 Treatment
attribute	pCO2_treatment	units	String	parts per million (ppm)
variable	approx_time		String
attribute	approx_time	bcodmo_name	String	time
attribute	approx_time	description	String	The approximate time of the given measurement; HH:MM
attribute	approx_time	long_name	String	Approx Time
attribute	approx_time	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/AHMSAA01/
attribute	approx_time	units	String	unitless
variable	elapsed_time		byte
attribute	elapsed_time	_FillValue	byte	127
attribute	elapsed_time	actual_range	byte	0, 25
attribute	elapsed_time	bcodmo_name	String	time_elapsed
attribute	elapsed_time	description	String	The time elapsed since the beginning of the 24 h monitoring.
attribute	elapsed_time	long_name	String	Elapsed Time
attribute	elapsed_time	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/
attribute	elapsed_time	units	String	hours
variable	date		String
attribute	date	bcodmo_name	String	date
attribute	date	description	String	The date the measurement was taken; YYYY/mm/dd
attribute	date	long_name	String	Date
attribute	date	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	date	time_precision	String	1970-01-01
attribute	date	units	String	unitless
variable	time2		String
attribute	time2	bcodmo_name	String	time
attribute	time2	description	String	The actual time that the measurements for that timepoint began; HH:MM
attribute	time2	long_name	String	Time
attribute	time2	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/AHMSAA01/
attribute	time2	units	String	unitless
variable	light		String
attribute	light	bcodmo_name	String	unknown
attribute	light	description	String	Indicates whether the lights in the incubator were turned on or off. When the lights were on the intensity was 470 umol photons/m-2/s PAR. When the lights were off the intensity was 0 umol photons/m-2/s PAR and the investigators used a red light when in the incubator to take samples.
attribute	light	long_name	String	Light
attribute	light	units	String	unitless
variable	pH		float
attribute	pH	_FillValue	float	NaN
attribute	pH	actual_range	float	7.683, 8.424
attribute	pH	bcodmo_name	String	pH
attribute	pH	colorBarMaximum	double	9.0
attribute	pH	colorBarMinimum	double	7.0
attribute	pH	description	String	pH measured on the total scale.
attribute	pH	long_name	String	Sea Water Ph Reported On Total Scale
attribute	pH	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/
attribute	pH	units	String	pH
variable	temperature		float
attribute	temperature	_FillValue	float	NaN
attribute	temperature	actual_range	float	15.9, 17.0
attribute	temperature	bcodmo_name	String	temperature
attribute	temperature	description	String	Temperature
attribute	temperature	long_name	String	Temperature
attribute	temperature	units	String	celsius
variable	salinity		float
attribute	salinity	_FillValue	float	NaN
attribute	salinity	actual_range	float	32.1, 32.6
attribute	salinity	bcodmo_name	String	sal
attribute	salinity	colorBarMaximum	double	37.0
attribute	salinity	colorBarMinimum	double	32.0
attribute	salinity	description	String	Salinity
attribute	salinity	long_name	String	Sea Water Practical Salinity
attribute	salinity	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PSALST01/
attribute	salinity	units	String	practical salinity units (PSU)
variable	inVivo_fluorescence		float
attribute	inVivo_fluorescence	_FillValue	float	NaN
attribute	inVivo_fluorescence	actual_range	float	2.76, 7.21
attribute	inVivo_fluorescence	bcodmo_name	String	fluorescence
attribute	inVivo_fluorescence	description	String	fluorescence to indicate relative chlorophyll-a concentration.
attribute	inVivo_fluorescence	long_name	String	In Vivo Fluorescence
attribute	inVivo_fluorescence	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/
attribute	inVivo_fluorescence	units	String	relative fluorescence units
variable	cell_density		int
attribute	cell_density	_FillValue	int	2147483647
attribute	cell_density	actual_range	int	31000, 88600
attribute	cell_density	bcodmo_name	String	cell_concentration
attribute	cell_density	description	String	cell density of the culture as measured by the Moxi Z Automated Cell Counter.
attribute	cell_density	long_name	String	Cell Density
attribute	cell_density	units	String	cells/mL
variable	mean_cellDiameter		float
attribute	mean_cellDiameter	_FillValue	float	NaN
attribute	mean_cellDiameter	actual_range	float	8.871, 11.045
attribute	mean_cellDiameter	bcodmo_name	String	unknown
attribute	mean_cellDiameter	description	String	mean cell diameter as measured by the Moxi Z Automated Cell Counter.
attribute	mean_cellDiameter	long_name	String	Mean Cell Diameter
attribute	mean_cellDiameter	units	String	microns (um)
variable	fluorescencePerCellDensity		float
attribute	fluorescencePerCellDensity	_FillValue	float	NaN
attribute	fluorescencePerCellDensity	actual_range	float	7.0E-5, 1.2E-4
attribute	fluorescencePerCellDensity	bcodmo_name	String	fluorescence
attribute	fluorescencePerCellDensity	description	String	Fluorescence divided by cell density to give an estimate of fluorescence per cell.
attribute	fluorescencePerCellDensity	long_name	String	Fluorescence Per Cell Density
attribute	fluorescencePerCellDensity	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/
attribute	fluorescencePerCellDensity	units	String	fluorescence/cell/mL
variable	PIC_ugCPerL		float
attribute	PIC_ugCPerL	_FillValue	float	NaN
attribute	PIC_ugCPerL	actual_range	float	373.42, 860.91
attribute	PIC_ugCPerL	bcodmo_name	String	PIC
attribute	PIC_ugCPerL	description	String	Particulate inorganic carbon concentration
attribute	PIC_ugCPerL	long_name	String	PIC Ug CPer L
attribute	PIC_ugCPerL	units	String	ugC/L
variable	PIC_pgCPerCell		float
attribute	PIC_pgCPerCell	_FillValue	float	NaN
attribute	PIC_pgCPerCell	actual_range	float	6.49, 21.54
attribute	PIC_pgCPerCell	bcodmo_name	String	PIC
attribute	PIC_pgCPerCell	description	String	Particulate inorganic carbon concentration with unit conversion to pg/mL-1 and divided by the cell density to give pg C per cell.
attribute	PIC_pgCPerCell	long_name	String	PIC Pg CPer Cell
attribute	PIC_pgCPerCell	units	String	pgC/cell
variable	NO3_NO2		float
attribute	NO3_NO2	_FillValue	float	NaN
attribute	NO3_NO2	actual_range	float	576.55, 879.48
attribute	NO3_NO2	bcodmo_name	String	NO3_NO2
attribute	NO3_NO2	colorBarMaximum	double	50.0
attribute	NO3_NO2	colorBarMinimum	double	0.0
attribute	NO3_NO2	description	String	Nitrate + Nitrite concentration measured at the University of California Santa Barbara.
attribute	NO3_NO2	long_name	String	Mole Concentration Of Nitrate In Sea Water
attribute	NO3_NO2	units	String	umol/kg
variable	NO3_UCSB		float
attribute	NO3_UCSB	_FillValue	float	NaN
attribute	NO3_UCSB	actual_range	float	614.87, 867.84
attribute	NO3_UCSB	bcodmo_name	String	NO3
attribute	NO3_UCSB	colorBarMaximum	double	50.0
attribute	NO3_UCSB	colorBarMinimum	double	0.0
attribute	NO3_UCSB	description	String	Nitrate concentration, measured at the University of California Santa Barbara
attribute	NO3_UCSB	long_name	String	Mole Concentration Of Nitrate In Sea Water
attribute	NO3_UCSB	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/NTRAIGGS/
attribute	NO3_UCSB	units	String	umol/kg
variable	NO3_Bigelow		float
attribute	NO3_Bigelow	_FillValue	float	NaN
attribute	NO3_Bigelow	actual_range	float	633.38, 866.12
attribute	NO3_Bigelow	bcodmo_name	String	NO3
attribute	NO3_Bigelow	colorBarMaximum	double	50.0
attribute	NO3_Bigelow	colorBarMinimum	double	0.0
attribute	NO3_Bigelow	description	String	Nitrate concentration, measured by Bigelow Analytical Services
attribute	NO3_Bigelow	long_name	String	Mole Concentration Of Nitrate In Sea Water
attribute	NO3_Bigelow	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/NTRAIGGS/
attribute	NO3_Bigelow	units	String	umol/kg
variable	PO4_UCSB		float
attribute	PO4_UCSB	_FillValue	float	NaN
attribute	PO4_UCSB	actual_range	float	17.1, 32.47
attribute	PO4_UCSB	bcodmo_name	String	PO4
attribute	PO4_UCSB	description	String	Phosphate concentration measured at the University of California Santa Barbara
attribute	PO4_UCSB	long_name	String	Mass Concentration Of Phosphate In Sea Water
attribute	PO4_UCSB	units	String	umol/kg
variable	PO4_Bigelow		float
attribute	PO4_Bigelow	_FillValue	float	NaN
attribute	PO4_Bigelow	actual_range	float	21.57, 32.73
attribute	PO4_Bigelow	bcodmo_name	String	PO4
attribute	PO4_Bigelow	description	String	Phosphate concentration measured by Bigelow Analytical Services
attribute	PO4_Bigelow	long_name	String	Mass Concentration Of Phosphate In Sea Water
attribute	PO4_Bigelow	units	String	umol/kg
variable	SiO4		float
attribute	SiO4	_FillValue	float	NaN
attribute	SiO4	actual_range	float	10.93, 24.0
attribute	SiO4	bcodmo_name	String	SiO4
attribute	SiO4	description	String	Silicate concentration measured at the University of California Santa Barbara
attribute	SiO4	long_name	String	Si O4
attribute	SiO4	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/SLCAAAZX/
attribute	SiO4	units	String	umol/kg
variable	mean_SiO4		float
attribute	mean_SiO4	_FillValue	float	NaN
attribute	mean_SiO4	actual_range	float	12.33, 21.59
attribute	mean_SiO4	bcodmo_name	String	SiO4
attribute	mean_SiO4	description	String	Silicate concentration estimated as the average of the values from the first and last timepoint. Biologically there should be no change in silicate concentration because coccolithophores do not take up silicate. However silicate values are needed to calculate the carbonate chemistry parameters in CO2sys so the average was used for timepoints when carbonate chemistry values were calculated.
attribute	mean_SiO4	long_name	String	Mean Si O4
attribute	mean_SiO4	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/SLCAAAZX/
attribute	mean_SiO4	units	String	umol/kg
variable	TA		float
attribute	TA	_FillValue	float	NaN
attribute	TA	actual_range	float	2050.8, 2174.9
attribute	TA	bcodmo_name	String	TALK
attribute	TA	description	String	Total alkalinity
attribute	TA	long_name	String	TA
attribute	TA	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/MDMAP014/
attribute	TA	units	String	ueq/kg
variable	DIC		float
attribute	DIC	_FillValue	float	NaN
attribute	DIC	actual_range	float	1751.9, 2006.8
attribute	DIC	bcodmo_name	String	DIC
attribute	DIC	description	String	Dissolved inorganic carbon concentration calculated using CO2sys
attribute	DIC	long_name	String	DIC
attribute	DIC	units	String	umol/kg
variable	pCO2		float
attribute	pCO2	_FillValue	float	NaN
attribute	pCO2	actual_range	float	201.9, 928.1
attribute	pCO2	bcodmo_name	String	pCO2
attribute	pCO2	description	String	Partial pressure of carbon dioxide in the water calculated using CO2sys. This calculated value more accurately represents the pCO2 of the culture than the nominal treatment value, which represents the pCO2 of the air bubbled into the culture.
attribute	pCO2	long_name	String	P CO2
attribute	pCO2	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/
attribute	pCO2	units	String	uatm
variable	HCO3		float
attribute	HCO3	_FillValue	float	NaN
attribute	HCO3	actual_range	float	1555.6, 1895.9
attribute	HCO3	bcodmo_name	String	bicarbonate
attribute	HCO3	description	String	Bicarbonate concentration calculated using CO2sys
attribute	HCO3	long_name	String	HCO3
attribute	HCO3	units	String	umol/kg
variable	CO3		float
attribute	CO3	_FillValue	float	NaN
attribute	CO3	actual_range	float	68.2, 215.5
attribute	CO3	bcodmo_name	String	carbonate
attribute	CO3	description	String	Carbonate concentration calculated using CO2sys
attribute	CO3	long_name	String	CO3
attribute	CO3	units	String	umol/kg
variable	CO2		float
attribute	CO2	_FillValue	float	NaN
attribute	CO2	actual_range	float	7.3, 33.6
attribute	CO2	bcodmo_name	String	pCO2
attribute	CO2	description	String	Carbon dioxide concentration calculated using CO2sys
attribute	CO2	long_name	String	CO2
attribute	CO2	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/
attribute	CO2	units	String	umol/kg
variable	omega_calcite		float
attribute	omega_calcite	_FillValue	float	NaN
attribute	omega_calcite	actual_range	float	1.66, 5.24
attribute	omega_calcite	bcodmo_name	String	Calcite Saturation State
attribute	omega_calcite	description	String	Saturation state of calcium carbonate with respect to calcite calculated using CO2sys
attribute	omega_calcite	long_name	String	Omega Calcite
attribute	omega_calcite	units	String	unitless
variable	time		double
attribute	time	_CoordinateAxisType	String	Time
attribute	time	actual_range	double	1.3740519E9, 1.37414298E9
attribute	time	axis	String	T
attribute	time	bcodmo_name	String	ISO_DateTime_UTC
attribute	time	description	String	Date/Time (UTC) ISO formatted; YYYY/mm/dd;HH:MM:SS
attribute	time	ioos_category	String	Time
attribute	time	long_name	String	ISO Date Time UTC
attribute	time	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/
attribute	time	source_name	String	ISO_DateTime_UTC
attribute	time	standard_name	String	time
attribute	time	time_origin	String	01-JAN-1970 00:00:00
attribute	time	time_precision	String	1970-01-01T00:00:00Z
attribute	time	units	String	seconds since 1970-01-01T00:00:00Z

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.