BCO-DMO ERDDAP
Accessing BCO-DMO data
log in    
Brought to you by BCO-DMO    

ERDDAP > info > bcodmo_dataset_660527

Grid
DAP
Data
Sub-
set
Table
DAP
Data
Make
A
Graph
W
M
S
Source
Data
Files
Acces-
sible
?
Title Sum-
mary
ISO,
Metadata
Back-
ground
Info
RSS E
mail
Institution Dataset ID
   set  data   graph     files  public Sediment geochemical and microbial activity data collected on R/V Oden along the East
Siberian Arctic Shelf from 2014 (ESAS Water Column Methane project)
   ?     I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_660527

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Acquisition methods are described in the following publication: Orcutt, B.N.
et al. 2005

Core sectioning, porewater\u00a0collection\u00a0and analysis

At each sampling site, sediment sub-samples were collected for porewater
analyses and at selected depths for microbial rate assays (AOM, anaerobic
oxidation of methane oxidation; methanogenesis (MOG) from bicarbonate and
acetate). Sediment was expelled from core liner using a hydraulic extruder
under anoxic conditions. The depth intervals for extrusion varied. At each
depth interval, a sub-sample was collected into a cut-off syringe for
dissolved methane concentration quantification. Another 5 mL\u00a0sub-
sample\u00a0was collected into pre-weighed and pre-combusted glass vial for
determination of porosity (determined by the change in weight after drying at
80 degrees celsius to a constant weight). The remaining material was used for
porewater extraction. Sample fixation and\u00a0analyses\u00a0for dissolved
constituents followed the methods of Joye et al. (2010).\u00a0

Microbial Activity Measurements\u00a0

To determine AOM and MOG rates, 8 to 12 sub-samples (5 cm3) were collected
from a core by manual insertion of a glass tube. For AOM, 100 uL of
dissolved\u00a014CH4\u00a0tracer (about 2,000,000 DPM as gas) was injected
into each core. Samples were incubated for 36 to 48 hours at\u00a0in
situ\u00a0temperature.\u00a0 Following incubation, samples were transferred to
20 mL glass vials containing 2 mL of 2M NaOH (which served to arrest
biological activity and fix\u00a014CO2\u00a0as\u00a014C-HCO3-).\u00a0 Each
vial was sealed with a\u00a0teflon-lined screw cap, vortexed to mix the sample
and base, and immediately frozen. Time zero samples were fixed immediately
after radiotracer injection. The specific activity of the tracer substrate
(14CH4) was determined by injecting 50 uL directly into scintillation cocktail
(Scintiverse BD) followed by liquid scintillation counting. The accumulation
of 14C product (14CO2) was determined by acid digestion following the method
of Joye et al. (2010).\u00a0 The AOM rate was calculated using equation 1:

AOM Rate = [CH4] x alphaCH4 /t x (a-14CO2/a-14CH4)\u00a0\u00a0
\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0 \u00a0(Eq. 1)

Here, the AOM Rate is expressed as nmol CH4 oxidized per cm3 sediment per day
(nmol\u00a0cm-3 d-1), [CH4] is the methane concentration (uM), alphaCH4 is the
isotope fractionation factor for AOM (1.06; (ALPERIN and REEBURGH, 1988)), t
is the incubation time (d), a-14CO2 is the activity of the product pool, and
a-14CH4 is the activity of the substrate pool. If methane concentration was
not available, the turnover time of the 14CH4 tracer is presented.

Rates of bicarbonate-based-methanogenesis and acetoclastic methanogenesis were
determined by incubating samples in gas-tight, closed-tube vessels without
headspace, to prevent the loss of gaseous 14CH4 product during sample
manipulation. These sample tubes were sealed using custom-designed plungers
(black Hungate stoppers with the lip removed containing a plastic
\u201ctail\u201d that was run through the stopper) were inserted at the base
of the tube; the sediment was then pushed via the plunger to the top of the
tube until a small amount protruded through the tube opening. A butyl rubber
septa\u00a0was\u00a0then eased into the tube opening to displace sediment in
contact with the atmosphere and close the tube, which was then sealed
with\u00a0a open-top\u00a0screw cap.\u00a0 The rubber materials used in these
assays were boiled in 1N NaOH for 1 hour, followed by several rinses in
boiling milliQ, to leach potentially toxic substances. \u00a0 \u00a0

A volume of radiotracer solution (100 uL of 14C-HCO3- tracer (~1 x
107\u00a0dpm\u00a0in slightly alkaline milliQ\u00a0water) or 1,2-14C-CH3COO-
tracer (~5 x 107\u00a0dpm\u00a0in slightly alkaline milliQ\u00a0water)) was
injected into each sample. Samples were incubated as described above and then
2 ml of 2N NaOH was injected through the top stopper into each sample to
terminate biological activity (time zero samples were fixed prior to tracer
injection).\u00a0 Samples were mixed to evenly distribute NaOH through the
sample.\u00a0 Production of 14CH4 was quantified by stripping methane from the
tubes with an air carrier, converting the 14CH4 to 14CO2 in a combustion
furnace, and subsequent trapping of the 14CO2 in NaOH as carbonate (CRAGG et
al., 1990; CRILL and MARTENS, 1986).\u00a0\u00a0Activity\u00a0of 14CO2 was
measured subsequently by liquid scintillation counting.\u00a0

The rates of Bi-MOG and Ac-MOG rates were calculated using equations 2 and 3,
respectively:

Bi-MOG Rate = [HCO3-] x alphaHCO3/t x\u00a0 (a-14CH4/a-H14CO3-) \u00a0 \u00a0
(Eq. 2)

Ac-MOG Rate = [CH3COO-] x alphaCH3COO-/t\u00a0 x\u00a0 (a-14CH4/a-14CH314COO-)
\u00a0 \u00a0 (Eq. 3)

Both rates are expressed as nmol HCO3- or CH3COO-, respectively, reduced cm-3
d-1, alphaHCO3\u00a0and alphaCH3COO- are the isotope fractionation factors for
MOG (assumed to be 1.06). [HCO3-] and [CH3COO-] are the\u00a0pore\u00a0water
bicarbonate (mM) and acetate (uM) concentrations, respectively, t is
incubation time (d), a-14CH4 is the activity of the product pool, and a-H14CO3
and a-14CH314COO are the activities of the substrate pools. If samples for
substrate concentration determination were not available, the substrate
turnover constant instead of the rate is presented.

For water column methane oxidation rate assays, triplicate 20 mL of live water
(in addition to one 20 mL sample which was killed with ethanol (750 uL of pure
EtOH) before tracer addition) were transferred from the CTD into serum vials.
Samples were amended with 2 x 10^6 DPM of 3H-labeled-methane tracer and
incubated for 24 to 72 hours (linearity of activity was tested and confirmed).
After incubation, samples were fixed with ethanol, as above, and a sub-sample
to determine total sample activity (3H-methane + 3H-water) was collected.
Next, the sample was purged with nitrogen to remove the 3H-methane tracer and
a sub-sample was amended with scintillation fluid and counted on a shipboard
scintillation counter to determine the activity of tracer in the product of
3H-methane oxidation, 3H-water. The methane oxidation rate was calculated as:

MOX Rate = [methane concentration in nM] x alphaCH4/t\u00a0 x\u00a0 (a-3H-
H2O/a-3H-CH4-) \u00a0 \u00a0 (Eq. 3)
attribute NC_GLOBAL awards_0_award_nid String 651766
attribute NC_GLOBAL awards_0_award_number String PLR-1023444
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=1023444 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Polar Programs
attribute NC_GLOBAL awards_0_funding_acronym String NSF PLR
attribute NC_GLOBAL awards_0_funding_source_nid String 490497
attribute NC_GLOBAL awards_0_program_manager String Henrietta N Edmonds
attribute NC_GLOBAL awards_0_program_manager_nid String 51517
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Arctic Sediment Data
S. Joye and V. Samarkin, PIs
Version 4 October 2016
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2016-10-04T21:16:40Z
attribute NC_GLOBAL date_modified String 2019-04-22T21:24:55Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.660527.1
attribute NC_GLOBAL Easternmost_Easting double 172.361
attribute NC_GLOBAL geospatial_lat_max double 78.942
attribute NC_GLOBAL geospatial_lat_min double 74.44
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double 172.361
attribute NC_GLOBAL geospatial_lon_min double 125.243
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/660527 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String CTD
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to collect water column samples
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 660538
attribute NC_GLOBAL instruments_0_description String The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column and permits scientists observe the physical properties in real time via a conducting cable connecting the CTD to a deck unit and computer on the ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast. This instrument designation is used when specific make and model are not known.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/130/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String CTD profiler
attribute NC_GLOBAL instruments_0_instrument_nid String 417
attribute NC_GLOBAL instruments_0_supplied_name String CTD
attribute NC_GLOBAL instruments_1_acronym String Multi Corer
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Core used in sampling
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 660536
attribute NC_GLOBAL instruments_1_description String The Multi Corer is a benthic coring device used to collect multiple, simultaneous, undisturbed sediment/water samples from the seafloor. Multiple coring tubes with varying sampling capacity depending on tube dimensions are mounted in a frame designed to sample the deep ocean seafloor. For more information, see Barnett et al. (1984) in Oceanologica Acta, 7, pp. 399-408.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/51/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Multi Corer
attribute NC_GLOBAL instruments_1_instrument_nid String 532
attribute NC_GLOBAL instruments_1_supplied_name String Multiple core
attribute NC_GLOBAL instruments_2_acronym String LSC
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Used to determine activity of tracer substrate
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 660537
attribute NC_GLOBAL instruments_2_description String Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used the quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB21/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Liquid Scintillation Counter
attribute NC_GLOBAL instruments_2_instrument_nid String 624
attribute NC_GLOBAL instruments_2_supplied_name String Liquid scintillation counter
attribute NC_GLOBAL keywords String aom, AOM_rate, bco, bco-dmo, biological, carbonate, ch3, ch4, chemical, co3, collection, collection_type, coo, data, dataset, depth, dmo, erddap, h14, latitude, longitude, management, mog, month, oceanography, office, preliminary, rate, sample, sample_ID, sed, sed_CH4, sediment, sediment_depth, srr, station, turnover, turnover_14_CH3COO_MOG, turnover_H14_CO3_MOG, turnover_SRR, type, year
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/660527/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/660527 (external link)
attribute NC_GLOBAL Northernmost_Northing double 78.942
attribute NC_GLOBAL param_mapping String {'660527': {'lat': 'master - latitude', 'sediment_depth': 'flag - depth', 'lon': 'master - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/660527/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Georgia
attribute NC_GLOBAL people_0_affiliation_acronym String UGA
attribute NC_GLOBAL people_0_person_name String Samantha B. Joye
attribute NC_GLOBAL people_0_person_nid String 51421
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of Georgia
attribute NC_GLOBAL people_1_affiliation_acronym String UGA
attribute NC_GLOBAL people_1_person_name String Vladimir Samarkin
attribute NC_GLOBAL people_1_person_nid String 641543
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of Georgia
attribute NC_GLOBAL people_2_affiliation_acronym String UGA
attribute NC_GLOBAL people_2_person_name String Samantha B. Joye
attribute NC_GLOBAL people_2_person_nid String 51421
attribute NC_GLOBAL people_2_role String Contact
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Hannah Ake
attribute NC_GLOBAL people_3_person_nid String 650173
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String ESAS Water Column Methane
attribute NC_GLOBAL projects_0_acronym String ESAS Water Column Methane
attribute NC_GLOBAL projects_0_description String We propose to study methane (CH4) release over the East Siberian Arctic shelf (ESAS), the largest (~10% of the world ocean shelf area) and the shallowest shelf (mean depth
attribute NC_GLOBAL projects_0_end_date String 2015-08
attribute NC_GLOBAL projects_0_geolocation String East Siberian Arctic Shelf
attribute NC_GLOBAL projects_0_name String The East Siberian Arctic Shelf as a Source of Atmospheric Methane: First Approach to Quantitative Assessment
attribute NC_GLOBAL projects_0_project_nid String 651769
attribute NC_GLOBAL projects_0_start_date String 2010-09
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 74.44
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String year,month,collection_type
attribute NC_GLOBAL summary String Sediment geochemical and microbial activity data collected on R/V Oden along the East Siberian Arctic Shelf from 2014 (ESAS Water Column Methane project)
attribute NC_GLOBAL title String Sediment geochemical and microbial activity data collected on R/V Oden along the East Siberian Arctic Shelf from 2014 (ESAS Water Column Methane project)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double 125.243
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable year   short  
attribute year _FillValue short 32767
attribute year actual_range short 2014, 2014
attribute year bcodmo_name String year
attribute year description String Year of sampling; YYYY
attribute year long_name String Year
attribute year nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/YEARXXXX/ (external link)
attribute year units String unitless
variable month   byte  
attribute month _FillValue byte 127
attribute month actual_range byte 7, 7
attribute month bcodmo_name String month
attribute month description String Month of sampling; mm
attribute month long_name String Month
attribute month nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/MNTHXXXX/ (external link)
attribute month units String unitless
variable collection_type   String  
attribute collection_type bcodmo_name String sampling_method
attribute collection_type description String Method used to collect samples
attribute collection_type long_name String Collection Type
attribute collection_type units String unitless
variable station   byte  
attribute station _FillValue byte 127
attribute station actual_range byte 1, 63
attribute station bcodmo_name String station
attribute station description String Station where sampling occurred
attribute station long_name String Station
attribute station units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 74.44, 78.942
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double 125.243, 172.361
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable sample_ID   String  
attribute sample_ID bcodmo_name String sample
attribute sample_ID description String PI issued sample ID number
attribute sample_ID long_name String Sample ID
attribute sample_ID nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute sample_ID units String unitless
variable sediment_depth   double  
attribute sediment_depth _FillValue double NaN
attribute sediment_depth actual_range double -5.0, 52.5
attribute sediment_depth bcodmo_name String depth_core
attribute sediment_depth description String Depth of sediment; negative depth values represent overlying water samples
attribute sediment_depth long_name String Sediment Depth
attribute sediment_depth units String centimeters
variable sed_CH4   float  
attribute sed_CH4 _FillValue float NaN
attribute sed_CH4 actual_range float 0.0, 53.52
attribute sed_CH4 bcodmo_name String unknown
attribute sed_CH4 description String Methane concentration in sediment
attribute sed_CH4 long_name String Sed CH4
attribute sed_CH4 units String microns (uM)
variable AOM_rate   float  
attribute AOM_rate _FillValue float NaN
attribute AOM_rate actual_range float 0.05, 247.62
attribute AOM_rate bcodmo_name String unknown
attribute AOM_rate description String Anaerobic Oxidation of Methane; CH4 oxidized in sediment per day; Rates were measured at stations 13 and 23 only.
attribute AOM_rate long_name String AOM Rate
attribute AOM_rate units String picomole per centimeter per day (pmol/cm/day)
variable turnover_14_CH3COO_MOG   String  
attribute turnover_14_CH3COO_MOG bcodmo_name String unknown
attribute turnover_14_CH3COO_MOG description String 14-CH3COO methanogenesis turnover; Rates were measured at stations 13 and 23 only.
attribute turnover_14_CH3COO_MOG long_name String Turnover 14 CH3 COO MOG
attribute turnover_14_CH3COO_MOG units String percent
variable turnover_H14_CO3_MOG   String  
attribute turnover_H14_CO3_MOG bcodmo_name String unknown
attribute turnover_H14_CO3_MOG description String H14-CO3 methanogenesis turnover; Rates were measured at stations 13 and 23 only.
attribute turnover_H14_CO3_MOG long_name String Turnover H14 CO3 MOG
attribute turnover_H14_CO3_MOG units String percent
variable turnover_SRR   float  
attribute turnover_SRR _FillValue float NaN
attribute turnover_SRR actual_range float 0.002, 0.127
attribute turnover_SRR bcodmo_name String unknown
attribute turnover_SRR description String Sulfate reduction methanogenesis turnover; Rates were measured at stations 13 and 23 only.
attribute turnover_SRR long_name String Turnover SRR
attribute turnover_SRR units String percent

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
ERDDAP, Version 2.02
Disclaimers | Privacy Policy | Contact