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     data   graph     files  public [Water Column Data - CTD] - Water column data from CTD casts along the East Siberian Arctic
Shelf on R/V Oden during 2011 (ESAS Water Column Methane project) (The East Siberian Arctic
Shelf as a Source of Atmospheric Methane: First Approach to Quantitative Assessment)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Acquisition methods are described in the following publication: Orcut, B. et
al. 2005

Core sectioning, porewater\u00a0collection\u00a0and analysis

At each sampling site, sediment sub-samples were collected for porewater
analyses and at selected depths for microbial rate assays (AOM, anaerobic
oxidation of methane oxidation; methanogenesis (MOG) from bicarbonate and
acetate). Sediment was expelled from core liner using a hydraulic extruder
under anoxic conditions. The depth intervals for extrusion varied. At each
depth interval, a sub-sample was collected into a cut-off syringe for
dissolved methane concentration quantification. Another 5 mL\u00a0sub-
sample\u00a0was collected into pre-weighed and pre-combusted glass vial for
determination of porosity (determined by the change in weight after drying at
80 degrees celsius to a constant weight). The remaining material was used for
porewater extraction. Sample fixation and\u00a0analyses\u00a0for dissolved
constituents followed the methods of Joye et al. (2010).\u00a0

Microbial Activity Measurements\u00a0

To determine AOM and MOG rates, 8 to 12 sub-samples (5 cm3) were collected
from a core by manual insertion of a glass tube. For AOM, 100 uL of
dissolved\u00a014CH4\u00a0tracer (about 2,000,000 DPM as gas) was injected
into each core. Samples were incubated for 36 to 48 hours at\u00a0in
situ\u00a0temperature.\u00a0 Following incubation, samples were transferred to
20 mL glass vials containing 2 mL of 2M NaOH (which served to arrest
biological activity and fix\u00a014CO2\u00a0as\u00a014C-HCO3-).\u00a0 Each
vial was sealed with a\u00a0teflon-lined screw cap, vortexed to mix the sample
and base, and immediately frozen. Time zero samples were fixed immediately
after radiotracer injection. The specific activity of the tracer substrate
(14CH4) was determined by injecting 50 uL directly into scintillation cocktail
(Scintiverse BD) followed by liquid scintillation counting. The accumulation
of 14C product (14CO2) was determined by acid digestion following the method
of Joye et al. (2010).\u00a0 The AOM rate was calculated using equation 1:

AOM Rate = [CH4] x alphaCH4 /t x (a-14CO2/a-14CH4)\u00a0\u00a0
\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0 \u00a0(Eq. 1)

Here, the AOM Rate is expressed as nmol CH4 oxidized per cm3 sediment per day
(nmol\u00a0cm-3 d-1), [CH4] is the methane concentration (uM), alphaCH4 is the
isotope fractionation factor for AOM (1.06; (ALPERIN and REEBURGH, 1988)), t
is the incubation time (d), a-14CO2 is the activity of the product pool, and
a-14CH4 is the activity of the substrate pool. If methane concentration was
not available, the turnover time of the 14CH4 tracer is presented.

Rates of bicarbonate-based-methanogenesis and acetoclastic methanogenesis were
determined by incubating samples in gas-tight, closed-tube vessels without
headspace, to prevent the loss of gaseous 14CH4 product during sample
manipulation. These sample tubes were sealed using custom-designed plungers
(black Hungate stoppers with the lip removed containing a plastic
\u201ctail\u201d that was run through the stopper) were inserted at the base
of the tube; the sediment was then pushed via the plunger to the top of the
tube until a small amount protruded through the tube opening. A butyl rubber
septa\u00a0was\u00a0then eased into the tube opening to displace sediment in
contact with the atmosphere and close the tube, which was then sealed
with\u00a0a open-top\u00a0screw cap.\u00a0 The rubber materials used in these
assays were boiled in 1N NaOH for 1 hour, followed by several rinses in
boiling milliQ, to leach potentially toxic substances. \u00a0 \u00a0

A volume of radiotracer solution (100 uL of 14C-HCO3- tracer (~1 x
107\u00a0dpm\u00a0in slightly alkaline milliQ\u00a0water) or 1,2-14C-CH3COO-
tracer (~5 x 107\u00a0dpm\u00a0in slightly alkaline milliQ\u00a0water)) was
injected into each sample. Samples were incubated as described above and then
2 ml of 2N NaOH was injected through the top stopper into each sample to
terminate biological activity (time zero samples were fixed prior to tracer
injection).\u00a0 Samples were mixed to evenly distribute NaOH through the
sample.\u00a0 Production of 14CH4 was quantified by stripping methane from the
tubes with an air carrier, converting the 14CH4 to 14CO2 in a combustion
furnace, and subsequent trapping of the 14CO2 in NaOH as carbonate (CRAGG et
al., 1990; CRILL and MARTENS, 1986).\u00a0\u00a0Activity\u00a0of 14CO2 was
measured subsequently by liquid scintillation counting.\u00a0

The rates of Bi-MOG and Ac-MOG rates were calculated using equations 2 and 3,
respectively:

Bi-MOG Rate = [HCO3-] x alphaHCO3/t x\u00a0 (a-14CH4/a-H14CO3-) \u00a0 \u00a0
(Eq. 2)

Ac-MOG Rate = [CH3COO-] x alphaCH3COO-/t\u00a0 x\u00a0 (a-14CH4/a-14CH314COO-)
\u00a0 \u00a0 (Eq. 3)

Both rates are expressed as nmol HCO3- or CH3COO-, respectively, reduced cm-3
d-1, alphaHCO3\u00a0and alphaCH3COO- are the isotope fractionation factors for
MOG (assumed to be 1.06). [HCO3-] and [CH3COO-] are the\u00a0pore\u00a0water
bicarbonate (mM) and acetate (uM) concentrations, respectively, t is
incubation time (d), a-14CH4 is the activity of the product pool, and a-H14CO3
and a-14CH314COO are the activities of the substrate pools. If samples for
substrate concentration determination were not available, the substrate
turnover constant instead of the rate is presented.

For water column methane oxidation rate assays, triplicate 20 mL of live water
(in addition to one 20 mL sample which was killed with ethanol (750 uL of pure
EtOH) before tracer addition) were transferred from the CTD into serum vials.
Samples were amended with 2 x 10^6 DPM of 3H-labeled-methane tracer and
incubated for 24 to 72 hours (linearity of activity was tested and confirmed).
After incubation, samples were fixed with ethanol, as above, and a sub-sample
to determine total sample activity (3H-methane + 3H-water) was collected.
Next, the sample was purged with nitrogen to remove the 3H-methane tracer and
a sub-sample was amended with scintillation fluid and counted on a shipboard
scintillation counter to determine the activity of tracer in the product of
3H-methane oxidation, 3H-water. The methane oxidation rate was calculated as:

MOX Rate = [methane concentration in nM] x alphaCH4/t\u00a0 x\u00a0 (a-3H-
H2O/a-3H-CH4-) \u00a0 \u00a0 (Eq. 3)
attribute NC_GLOBAL awards_0_award_nid String 651766
attribute NC_GLOBAL awards_0_award_number String PLR-1023444
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=1023444 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Polar Programs
attribute NC_GLOBAL awards_0_funding_acronym String NSF PLR
attribute NC_GLOBAL awards_0_funding_source_nid String 490497
attribute NC_GLOBAL awards_0_program_manager String Henrietta N Edmonds
attribute NC_GLOBAL awards_0_program_manager_nid String 51517
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Water Column Data
S. Joye and V. Samarkin, PIs
Version 4 October 2016
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2016-10-04T23:10:06Z
attribute NC_GLOBAL date_modified String 2019-04-22T21:00:22Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.660543.1
attribute NC_GLOBAL Easternmost_Easting double 178.9479
attribute NC_GLOBAL geospatial_lat_max double 77.3829
attribute NC_GLOBAL geospatial_lat_min double 65.0835
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double 178.9479
attribute NC_GLOBAL geospatial_lon_min double 125.0406
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 651.0
attribute NC_GLOBAL geospatial_vertical_min double 10.0
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/660543 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String CTD
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to collect water column samples
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 660553
attribute NC_GLOBAL instruments_0_description String The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column and permits scientists observe the physical properties in real time via a conducting cable connecting the CTD to a deck unit and computer on the ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast. This instrument designation is used when specific make and model are not known.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/130/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String CTD profiler
attribute NC_GLOBAL instruments_0_instrument_nid String 417
attribute NC_GLOBAL instruments_0_supplied_name String CTD
attribute NC_GLOBAL keywords String ammonium, bco, bco-dmo, biological, ch4, chemical, chemistry, commerce, concentration, data, dataset, date, density, department, depth, depth_max, depth_sample, dmo, doc, don, dop, earth, Earth Science > Oceans > Ocean Chemistry > pH, Earth Science > Oceans > Ocean Chemistry > Phosphate, Earth Science > Oceans > Ocean Chemistry > Silicate, Earth Science > Oceans > Salinity/Density > Salinity, erddap, latitude, longitude, management, mass, mass_concentration_of_phosphate_in_sea_water, mass_concentration_of_silicate_in_sea_water, mox, nh4, nitrite, no2, nox, ocean, oceanography, oceans, office, phosphate, po4, practical, preliminary, reported, salinity, scale, science, sea, sea_water_ph_reported_on_total_scale, sea_water_practical_salinity, seawater, silicate, so4, station, tdn, tdp, temperature, time, total, water
attribute NC_GLOBAL keywords_vocabulary String GCMD Science Keywords
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/660543/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/660543 (external link)
attribute NC_GLOBAL Northernmost_Northing double 77.3829
attribute NC_GLOBAL param_mapping String {'660543': {'lat': 'master - latitude', 'depth_max': 'flag - depth', 'lon': 'master - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/660543/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Georgia
attribute NC_GLOBAL people_0_affiliation_acronym String UGA
attribute NC_GLOBAL people_0_person_name String Samantha B. Joye
attribute NC_GLOBAL people_0_person_nid String 51421
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of Georgia
attribute NC_GLOBAL people_1_affiliation_acronym String UGA
attribute NC_GLOBAL people_1_person_name String Vladimir Samarkin
attribute NC_GLOBAL people_1_person_nid String 641543
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of Georgia
attribute NC_GLOBAL people_2_affiliation_acronym String UGA
attribute NC_GLOBAL people_2_person_name String Samantha B. Joye
attribute NC_GLOBAL people_2_person_nid String 51421
attribute NC_GLOBAL people_2_role String Contact
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Hannah Ake
attribute NC_GLOBAL people_3_person_nid String 650173
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String ESAS Water Column Methane
attribute NC_GLOBAL projects_0_acronym String ESAS Water Column Methane
attribute NC_GLOBAL projects_0_description String We propose to study methane (CH4) release over the East Siberian Arctic shelf (ESAS), the largest (~10% of the world ocean shelf area) and the shallowest shelf (mean depth
attribute NC_GLOBAL projects_0_end_date String 2015-08
attribute NC_GLOBAL projects_0_geolocation String East Siberian Arctic Shelf
attribute NC_GLOBAL projects_0_name String The East Siberian Arctic Shelf as a Source of Atmospheric Methane: First Approach to Quantitative Assessment
attribute NC_GLOBAL projects_0_project_nid String 651769
attribute NC_GLOBAL projects_0_start_date String 2010-09
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 65.0835
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Water column data from CTD casts along the East Siberian Arctic Shelf on R/V Oden during 2011 (ESAS Water Column Methane project)
attribute NC_GLOBAL title String [Water Column Data - CTD] - Water column data from CTD casts along the East Siberian Arctic Shelf on R/V Oden during 2011 (ESAS Water Column Methane project) (The East Siberian Arctic Shelf as a Source of Atmospheric Methane: First Approach to Quantitative Assessment)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double 125.0406
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable station   byte  
attribute station _FillValue byte 127
attribute station actual_range byte 2, 105
attribute station bcodmo_name String station
attribute station description String Station where sampling occurred
attribute station long_name String Station
attribute station units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 65.0835, 77.3829
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double 125.0406, 178.9479
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable date   String  
attribute date bcodmo_name String date
attribute date description String Date of sampling; YYYY/mm/dd
attribute date long_name String Date
attribute date nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute date source_name String date
attribute date time_precision String 1970-01-01
attribute date units String unitless
variable depth   double  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth _FillValue double NaN
attribute depth actual_range double 10.0, 651.0
attribute depth axis String Z
attribute depth bcodmo_name String depth_max
attribute depth colorBarMaximum double 8000.0
attribute depth colorBarMinimum double -8000.0
attribute depth colorBarPalette String TopographyDepth
attribute depth description String Max depth on site where sampling occurred
attribute depth ioos_category String Location
attribute depth long_name String Depth
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable depth_sample   float  
attribute depth_sample _FillValue float NaN
attribute depth_sample actual_range float 2.8, 638.5
attribute depth_sample bcodmo_name String depth
attribute depth_sample colorBarMaximum double 8000.0
attribute depth_sample colorBarMinimum double -8000.0
attribute depth_sample colorBarPalette String TopographyDepth
attribute depth_sample description String Depth where sampling occurred
attribute depth_sample long_name String Depth
attribute depth_sample nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute depth_sample standard_name String depth
attribute depth_sample units String meters
variable temperature   float  
attribute temperature _FillValue float NaN
attribute temperature actual_range float -1.7, 6.0
attribute temperature bcodmo_name String temperature
attribute temperature description String Temperature where sampling occurred
attribute temperature long_name String Temperature
attribute temperature units String celsius
variable pH   float  
attribute pH _FillValue float NaN
attribute pH actual_range float 7.4, 8.3
attribute pH bcodmo_name String pH
attribute pH colorBarMaximum double 9.0
attribute pH colorBarMinimum double 7.0
attribute pH description String pH of water sample
attribute pH long_name String Sea Water Ph Reported On Total Scale
attribute pH nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute pH units String pH
variable salinity   float  
attribute salinity _FillValue float NaN
attribute salinity actual_range float 14.1, 34.9
attribute salinity bcodmo_name String sal
attribute salinity colorBarMaximum double 37.0
attribute salinity colorBarMinimum double 32.0
attribute salinity description String Salinity of water sample
attribute salinity long_name String Sea Water Practical Salinity
attribute salinity nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PSALST01/ (external link)
attribute salinity units String practical salinity units (PSU)
variable Cl   short  
attribute Cl _FillValue short 32767
attribute Cl actual_range short 228, 570
attribute Cl bcodmo_name String unknown
attribute Cl description String Chlorine concentration
attribute Cl long_name String CL
attribute Cl units String millimoles (mM)
variable SO4   float  
attribute SO4 _FillValue float NaN
attribute SO4 actual_range float 14.6, 28.2
attribute SO4 bcodmo_name String SO4
attribute SO4 description String Sulfate concentration
attribute SO4 long_name String SO4
attribute SO4 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/SPHTMAXX/ (external link)
attribute SO4 units String millimoles (mM)
variable DOC   short  
attribute DOC _FillValue short 32767
attribute DOC actual_range short 32, 364
attribute DOC bcodmo_name String DOC
attribute DOC description String Dissolved organic carbon concentration
attribute DOC long_name String DOC
attribute DOC nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CORGZZZX/ (external link)
attribute DOC units String micromoles (uM)
variable TDN   byte  
attribute TDN _FillValue byte 127
attribute TDN actual_range byte 6, 42
attribute TDN bcodmo_name String Total Dissolved Nitrogren
attribute TDN description String Total dissolved nitrogen concentration
attribute TDN long_name String TDN
attribute TDN units String micromoles (uM)
variable DON   byte  
attribute DON _FillValue byte 127
attribute DON actual_range byte 2, 13
attribute DON bcodmo_name String Dissolved Organic Nitrogen
attribute DON description String Dissolved organic nitrogen concentration
attribute DON long_name String DON
attribute DON units String micromoles (uM)
variable Nox   String  
attribute Nox bcodmo_name String unknown
attribute Nox description String Mono-nitrogen oxide concentration
attribute Nox long_name String Nox
attribute Nox units String micromoles (uM)
variable NO2   String  
attribute NO2 bcodmo_name String NO2
attribute NO2 description String Nitrite concentration
attribute NO2 long_name String NO2
attribute NO2 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/NTRIAAZX/ (external link)
attribute NO2 units String micromoles (uM)
variable NH4   String  
attribute NH4 bcodmo_name String Ammonium
attribute NH4 description String Ammonium concentration
attribute NH4 long_name String NH4
attribute NH4 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/AMONAAZX/ (external link)
attribute NH4 units String micromoles (uM)
variable TDP   float  
attribute TDP _FillValue float NaN
attribute TDP actual_range float 0.3, 5.3
attribute TDP bcodmo_name String Total Dissolved Phosphorus
attribute TDP description String Total dissolved phosphorus concetration
attribute TDP long_name String TDP
attribute TDP units String micromoles (uM)
variable DOP   String  
attribute DOP bcodmo_name String Dissolved Organic Phosphorus
attribute DOP description String Dissolved organic phosphorus concentration
attribute DOP long_name String DOP
attribute DOP units String micromoles (uM)
variable PO4   float  
attribute PO4 _FillValue float NaN
attribute PO4 actual_range float 0.2, 4.2
attribute PO4 bcodmo_name String PO4
attribute PO4 description String Phosphate concentration
attribute PO4 long_name String Mass Concentration Of Phosphate In Sea Water
attribute PO4 units String micromoles (uM)
variable Si   float  
attribute Si _FillValue float NaN
attribute Si actual_range float 0.5, 113.5
attribute Si bcodmo_name String Si
attribute Si description String Silicon concentation
attribute Si long_name String Mass Concentration Of Silicate In Sea Water
attribute Si units String micromoles (uM)
variable CH4   float  
attribute CH4 _FillValue float NaN
attribute CH4 actual_range float 1.8, 191.4
attribute CH4 bcodmo_name String unknown
attribute CH4 description String Methane concentration
attribute CH4 long_name String CH4
attribute CH4 units String micromoles (uM)
variable MOX   float  
attribute MOX _FillValue float NaN
attribute MOX actual_range float 0.001, 0.76
attribute MOX bcodmo_name String unknown
attribute MOX description String Methane oxidation rate
attribute MOX long_name String MOX
attribute MOX units String nanomole per liter per day (nmol/L/d)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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