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     data   graph     files  public Nutrient concentrations, microbiology (Vibrio abundance), trace metals, and environmental
conditions from collection sites in the Florida Keys National Marine Sanctuary, 2014-2016
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Samples were collected offshore from Alligator Reef (2014) and Looe Key Reef
(2015-2016). Measurements included temperature, salinity, pH, abundance of
Vibrio, chlorophyll-a, DOC, TDN, DON, NH4, NO3, NO2, Orthophosphate ,SiO4, and
dFe.

Dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) were
determined using the filtrate of water samples that were passed through
precombusted 25 mm GF/F filters and stored frozen (-20C) until analysis.\u00a0
Samples were subsequently analyzed using the High Temperature Catalytic
Oxidation method on a Shimadzu TOC-Vs analyzer with nitrogen module.\u00a0
Standard curves were run twice daily using a DIW blank and five concentrations
of either acid potassium phthalate solution or potassium nitrate for DOC and
TDN, respectively.\u00a0 Three to five subsamples were taken from each
standard and water sample and injected in sequence.\u00a0 Reagent grade
glucosamine was used as a laboratory check standard and inserted throughout
each run, as were Certified Reference Material Program (CRMP) deep-water
standards of known DOC/TDN concentration.\u00a0

Nutrients data went through internal lab QAQC process. BDL means below
detection limit. The method detection limit (MDL) was determined using 9
samples on two different runs and correct student-T value.
attribute NC_GLOBAL awards_0_award_nid String 553932
attribute NC_GLOBAL awards_0_award_number String OCE-1357423
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1357423 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Seeded microcosm experiments: collection data
Nutrient concentrations, microbiology (Vibrio abundance), trace metals, and environmental conditions
PI's: E. Lipp (UGA), W. Landing (FSU) E. Ottesen (UGA), M. Wetz (TAMU)
version: 2018-05-03 [added 2016 data]
replaces version: 2016-11-01
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL dataset_current_state String Final with updates expected
attribute NC_GLOBAL date_created String 2016-11-01T20:39:57Z
attribute NC_GLOBAL date_modified String 2020-06-03T14:11:32Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.663707.1
attribute NC_GLOBAL Easternmost_Easting double -80.68487
attribute NC_GLOBAL geospatial_lat_max double 24.74287
attribute NC_GLOBAL geospatial_lat_min double 24.54322
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -80.68487
attribute NC_GLOBAL geospatial_lon_min double -81.41115
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 317.0
attribute NC_GLOBAL geospatial_vertical_min double 28.0
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/663707 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Gas Analyzer
attribute NC_GLOBAL instruments_0_dataset_instrument_description String To measure dissolved organic nitrogen
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 663737
attribute NC_GLOBAL instruments_0_description String Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.
attribute NC_GLOBAL instruments_0_instrument_name String Gas Analyzer
attribute NC_GLOBAL instruments_0_instrument_nid String 720
attribute NC_GLOBAL instruments_0_supplied_name String chemoluminescence gas analyzer
attribute NC_GLOBAL instruments_1_dataset_instrument_description String To measure colony counts
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 663736
attribute NC_GLOBAL instruments_1_description String Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: https://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.
attribute NC_GLOBAL instruments_1_instrument_name String plate reader
attribute NC_GLOBAL instruments_1_instrument_nid String 528693
attribute NC_GLOBAL keywords String altimetry, ammonia, ammonium, bco, bco-dmo, biological, chemical, chemistry, Chl_a, chlorophyll, commerce, concentration, concentration_of_chlorophyll_in_sea_water, data, dataset, date, department, depth, dFe, dmo, doc, don, earth, Earth Science > Oceans > Ocean Chemistry > Ammonia, Earth Science > Oceans > Ocean Chemistry > Chlorophyll, Earth Science > Oceans > Ocean Chemistry > pH, erddap, laboratory, latitude, local, longitude, management, mole, mole_concentration_of_ammonium_in_sea_water, mole_concentration_of_nitrite_in_sea_water, nh4, nitrate, nitrite, NO2, no3, notes, ocean, oceanography, oceans, office, orthophosphate, preliminary, reported, sal, sample, satellite, scale, science, sea, sea_water_ph_reported_on_total_scale, seawater, SiO4, start, station, station_notes, tdn, temperature, time, time_start_local, total, vibrio, water
attribute NC_GLOBAL keywords_vocabulary String GCMD Science Keywords
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/663707/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/663707 (external link)
attribute NC_GLOBAL Northernmost_Northing double 24.74287
attribute NC_GLOBAL param_mapping String {'663707': {'lat': 'master - latitude', 'depth': 'master - depth', 'lon': 'master - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/663707/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Georgia
attribute NC_GLOBAL people_0_affiliation_acronym String UGA
attribute NC_GLOBAL people_0_person_name String Erin K. Lipp
attribute NC_GLOBAL people_0_person_nid String 553935
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Florida State University
attribute NC_GLOBAL people_1_affiliation_acronym String FSU - EOAS
attribute NC_GLOBAL people_1_person_name String William M. Landing
attribute NC_GLOBAL people_1_person_nid String 51302
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of Georgia
attribute NC_GLOBAL people_2_affiliation_acronym String UGA
attribute NC_GLOBAL people_2_person_name String Elizabeth Ottesen
attribute NC_GLOBAL people_2_person_nid String 553937
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Texas A&M University
attribute NC_GLOBAL people_3_affiliation_acronym String TAMU
attribute NC_GLOBAL people_3_person_name String Michael Wetz
attribute NC_GLOBAL people_3_person_nid String 553945
attribute NC_GLOBAL people_3_role String Co-Principal Investigator
attribute NC_GLOBAL people_3_role_type String originator
attribute NC_GLOBAL people_4_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_4_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_4_person_name String Nancy Copley
attribute NC_GLOBAL people_4_person_nid String 50396
attribute NC_GLOBAL people_4_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_4_role_type String related
attribute NC_GLOBAL project String Vibrio-dust deposition
attribute NC_GLOBAL projects_0_acronym String Vibrio-dust deposition
attribute NC_GLOBAL projects_0_description String Description from NSF award abstract:
Dust and mineral aerosols are a significant source of micro and macronutrients to oligotrophic ocean surface waters. Evidence is growing that heterotrophic microbes may play key roles in processing deposited minerals and nutrients. Yet it is not known which components of dust stimulate the heterotrophic bacteria, which cellular mechanisms are responsible for the utilization of those components and how the activity of these bacteria affect the availability and utilization of dust-derived minerals and nutrients by marine autotrophs. Knowledge of these factors is key to understanding how dust deposition impacts carbon cycles and for predicting the response of tropical oceans to future changes in the frequency and intensity of dust deposition events. The objective of this project is to examine the specific effects of aeolian dust on heterotrophic microbes in a tropical marine system under controlled conditions. The central hypothesis is that in oligotrophic tropical systems numerically minor opportunistic bacteria are the first responders to influx of dust constituents and respond primarily by rapidly accessing soluble trace metals and limiting nutrients that are deposited with Saharan dust. The project will focus on two specific aims: 1) Quantify changes in community structure, composition and transcriptional activity among marine microbial populations upon exposure to dust, and 2) Identify key components in Saharan dust aerosols that stimulate or repress growth and/or activity in Vibrio, a model opportunistic marine heterotrophic group. The study will use a series of controlled experiments designed to identify and quantify heterotrophic microbial response to dust deposition events using both natural communities and model bacteria (Vibrio) through metagenomics, transcriptomics and atmospheric and marine biogeochemical techniques. This innovative approach will identify the most critical (reactive) components leached from dust aerosols on the microbial community as well as elucidate potential mechanisms of response.
There is great interest in the biological response to dust aerosols given its potentially large influence on biogeochemical cycling, but there has been relatively little work that has addressed the mechanisms of response (especially among the heterotrophic microbial fraction) or identified the relative importance of specific constituents of dust aerosols. A detailed framework for microbial response (focusing on opportunistic heterotrophs) will facilitate efforts to link autotrophic and heterotrophic processing. This contribution is significant because it will provide one of the first end-to-end (chemistry to physiology to ecology) mechanistic pathways for marine biological response to desert dust aerosols.
attribute NC_GLOBAL projects_0_end_date String 2017-03
attribute NC_GLOBAL projects_0_geolocation String Florida Keys, FL, USA
attribute NC_GLOBAL projects_0_name String Vibrio as a model microbe for opportunistic heterotrophic response to Saharan dust deposition events in marine waters
attribute NC_GLOBAL projects_0_project_nid String 553933
attribute NC_GLOBAL projects_0_start_date String 2014-04
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 24.54322
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Nutrient concentrations, microbiology (Vibrio abundance), trace metals, and environmental conditions from collection sites in the Florida Keys National Marine Sanctuary, 2014-2016.
attribute NC_GLOBAL title String Nutrient concentrations, microbiology (Vibrio abundance), trace metals, and environmental conditions from collection sites in the Florida Keys National Marine Sanctuary, 2014-2016
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -81.41115
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5
variable sample   String  
attribute sample bcodmo_name String sample
attribute sample description String sample identifier
attribute sample long_name String Sample
attribute sample nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute sample units String unitless
variable date   String  
attribute date bcodmo_name String date
attribute date description String sampling date formatted as yyyy-mm-dd
attribute date long_name String Date
attribute date nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute date source_name String date
attribute date time_precision String 1970-01-01
attribute date units String year-month-day
variable time_start_local   String  
attribute time_start_local bcodmo_name String time_start_local
attribute time_start_local description String local start time formatted as HH:MM
attribute time_start_local long_name String Time Start Local
attribute time_start_local units String hours:minutes
variable depth   double  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth _FillValue double NaN
attribute depth actual_range double 28.0, 317.0
attribute depth axis String Z
attribute depth bcodmo_name String depth
attribute depth colorBarMaximum double 8000.0
attribute depth colorBarMinimum double -8000.0
attribute depth colorBarPalette String TopographyDepth
attribute depth description String depth
attribute depth ioos_category String Location
attribute depth long_name String Depth
attribute depth nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable location   String  
attribute location bcodmo_name String site
attribute location description String sample collection location
attribute location long_name String Location
attribute location units String unitless
variable station_notes   String  
attribute station_notes bcodmo_name String comment
attribute station_notes description String comments pertaining to the station
attribute station_notes long_name String Station Notes
attribute station_notes units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 24.54322, 24.74287
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String latitude; north is positive
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -81.41115, -80.68487
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String longitude; east is positive
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable temp   float  
attribute temp _FillValue float NaN
attribute temp actual_range float 25.0, 29.0
attribute temp bcodmo_name String temperature
attribute temp description String temperature
attribute temp long_name String Temperature
attribute temp nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/ (external link)
attribute temp units String degrees Celsius
variable sal   byte  
attribute sal _FillValue byte 127
attribute sal actual_range byte 35, 36
attribute sal bcodmo_name String sal
attribute sal description String salinity
attribute sal long_name String Sal
attribute sal nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PSALST01/ (external link)
attribute sal units String Practical Salinity Units (PSU)
variable pH   float  
attribute pH _FillValue float NaN
attribute pH actual_range float 7.86, 8.01
attribute pH bcodmo_name String pH
attribute pH colorBarMaximum double 9.0
attribute pH colorBarMinimum double 7.0
attribute pH description String pH: The measure of the acidity or basicity of an aqueous solution
attribute pH long_name String Sea Water Ph Reported On Total Scale
attribute pH nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute pH units String unitless; pH scale
variable Vibrio   float  
attribute Vibrio _FillValue float NaN
attribute Vibrio actual_range float 0.0, 1250.0
attribute Vibrio bcodmo_name String unknown
attribute Vibrio description String Vibrio colony concentration. CFU/ml determined by spread on TCBS agar in triplicate for each experimental replicate and counting green and yellow colonies after 18 - 24 incubation at 30 C. limit of detection was 3.3 CFU/ml (determined using 100 ul spread volume in triplicate); 0.0 = below detection limit.
attribute Vibrio long_name String Vibrio
attribute Vibrio units String colony forming units/milliliter (CFU/ml)
variable Chl_a   float  
attribute Chl_a _FillValue float NaN
attribute Chl_a actual_range float 0.1, 1.04
attribute Chl_a bcodmo_name String chlorophyll a
attribute Chl_a colorBarMaximum double 30.0
attribute Chl_a colorBarMinimum double 0.03
attribute Chl_a colorBarScale String Log
attribute Chl_a description String Chlorophyll-a concentration determined by acetone freeze thaw using EPA method 445.0 (non-acidification); bdl for below detection limit.
attribute Chl_a long_name String Concentration Of Chlorophyll In Sea Water
attribute Chl_a nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/ (external link)
attribute Chl_a units String micrograms/liter (ug/ml)
variable DOC   float  
attribute DOC _FillValue float NaN
attribute DOC actual_range float 58.4, 181.2
attribute DOC bcodmo_name String DOC
attribute DOC description String Dissolved organic carbon concentration: determined using oxidative high temperature combustion-infrared analysis. MDL is 11.16 micromol/L.
attribute DOC long_name String DOC
attribute DOC nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CORGZZZX/ (external link)
attribute DOC units String micromoles/liter (ug/ml)
variable TDN   float  
attribute TDN _FillValue float NaN
attribute TDN actual_range float 0.0, 14.6
attribute TDN bcodmo_name String Total Dissolved Nitrogren
attribute TDN description String Total dissolved nitrogen concentration determined using oxidative high temperature combustion-infrared analysis.
attribute TDN long_name String TDN
attribute TDN units String micromoles/liter (ug/ml)
variable DON   float  
attribute DON _FillValue float NaN
attribute DON actual_range float 1.4, 12.8
attribute DON bcodmo_name String Dissolved Organic Nitrogen
attribute DON description String Dissolved organic nitrogen concentration; MDL is 5.38 micromol/L (determined using 9 samples on two different runs and correct student-T value).  bdl = below detection limit.
attribute DON long_name String DON
attribute DON units String micromoles/liter (ug/ml)
variable NH4   float  
attribute NH4 _FillValue float NaN
attribute NH4 actual_range float 0.6, 7.95
attribute NH4 bcodmo_name String Ammonium
attribute NH4 colorBarMaximum double 5.0
attribute NH4 colorBarMinimum double 0.0
attribute NH4 description String Ammonium concentration determined by the automated phenate method 4500-NH3G. (20th Edition Std. Meth.); MDL is 0.3 micro gram/L (determined using 9 samples on two different runs and correct student-T value).  bdl = below detection limit.
attribute NH4 long_name String Mole Concentration Of Ammonium In Sea Water
attribute NH4 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/AMONAAZX/ (external link)
attribute NH4 units String micromoles/liter (ug/ml)
variable NO3   String  
attribute NO3 bcodmo_name String NO3
attribute NO3 description String Nitrate concentration determined by the automated cadmium reduction method 4500-NO3- F (20th Edition Std. Meth.); MDL is 0.3 micro gram/L (determined using 9 samples on two different runs and correct student-T value). bdl = below detection limit.
attribute NO3 long_name String NO3
attribute NO3 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/NTRAIGGS/ (external link)
attribute NO3 units String micromoles/liter (ug/ml)
variable NO2   float  
attribute NO2 _FillValue float NaN
attribute NO2 actual_range float 0.02, 0.2
attribute NO2 bcodmo_name String NO2
attribute NO2 colorBarMaximum double 1.0
attribute NO2 colorBarMinimum double 0.0
attribute NO2 description String Nitrite concentration determined as with Nitrate without running the sample through a cadmium column (20th Edition Std. Meth.); MDL is 0.1 micro gram/L (determined using 9 samples on two different runs and correct student-T value). bdl = below detection limit.
attribute NO2 long_name String Mole Concentration Of Nitrite In Sea Water
attribute NO2 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/NTRIAAZX/ (external link)
attribute NO2 units String micromoles/liter (ug/ml)
variable Orthophosphate   String  
attribute Orthophosphate bcodmo_name String PO4
attribute Orthophosphate description String Orthophosphate concentration determined by the automated ascorbic acid reduction method 4500-P F. (20th Edition Std. Meth.); MDL is 0.2 micro gram/L (determined using 9 samples on two different runs and correct student-T value). bdl = below detection limit.
attribute Orthophosphate long_name String Orthophosphate
attribute Orthophosphate units String micromoles/liter (ug/ml)
variable SiO4   String  
attribute SiO4 bcodmo_name String SiO4
attribute SiO4 description String Silicate concentration determined by the automated molybdate-reactive silica method 4500-SiO2 E. (20th Edition Std. Meth.); MDL is 0.3 micro gram/L (determined using 9 samples on two different runs and correct student-T value). bdl = below detection limit.
attribute SiO4 long_name String Si O4
attribute SiO4 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/SLCAAAZX/ (external link)
attribute SiO4 units String micromoles/liter (ug/ml)
variable dFe   float  
attribute dFe _FillValue float NaN
attribute dFe actual_range float 0.71, 5.662
attribute dFe bcodmo_name String Fe
attribute dFe description String dissolved iron concentration determined in the 0.2 um filtered fraction using ICP-MSas described in Milne et al. 2010. Analytica Chimera Acta 665: 200-207
attribute dFe long_name String D Fe
attribute dFe units String nanomoles/liter (ug/ml)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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