ERDDAP > info > bcodmo_dataset_664013

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	Light-dark calcification rates of Pleurochrysis carterae analyzed at Bigelow Laboratory in 2013 (OA Copes Coccoliths project)		I   M	background			BCO-DMO	bcodmo_dataset_664013

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Cultures:\u00a0Pleurochrysis carterae\u00a0cultures were maintained in exponential growth phase under axenic conditions in semi-continuous batch culture using L1-Si media prepared on 0.2 um-filtered, UV-sterilized, autoclaved seawater.\u00a0 Cultures were acclimated to one of three\u00a0pCO2\u00a0treatments for > 9 generations before experiments were performed.\u00a0 Cultures were maintained in an incubator at 16.5 +/- 0.5 deg C and 470 umol photons/m-2/s\u00a0PAR. pCO2: Carbonate chemistry was manipulated by bubbling cultures and prepared media with 500 mL/min\u00a0with 0.2 um-filtered 280, 380, or 750 ppm\u00a0pCO2\u00a0air.\u00a0 The\u00a0pCO2\u00a0levels of the treatment air were established using two mass flow controllers (Aalborg, Orangeburg, NY, USA) for each treatment to precisely mix in-house compressed air and pure CO2\u00a0(Maine Oxy, Auburn, ME, USA).\u00a0 The in-house compressed air was stripped of CO2\u00a0to less than 10 ppm CO2\u00a0using a Puregas VCD CO2\u00a0Adsorber (Puregas, LLC, Broomfield, CO, USA).\u00a0 The\u00a0pCO2\u00a0of the gas mixtures was stable to +/- 8 ppm.\u00a0\u00a0pCO2\u00a0values of the cultures may be different than the target levels due to biological activity. Dissolution of existing coccoliths:\u00a0 Coccoliths were dissolved by 1.75 M HCl to drop the pH to 5.5 for 2 min.\u00a0 Following the dissolution (de- lithing), 1.75 M NaOH was added to bring the pH back to the respective starting pH.\u00a0 Dissolution of coccoliths was immediately confirmed by looking at the cells under cross-polarized light microscopy to verify the absence of birefringence indicative of CaCO3.\u00a0 Dissolution was further confirmed by filtering the acidified/neutralized sample onto a 0.4 um polycarbonate filter.\u00a0 Filters were mounted on stubs, sputter-coated with gold using a Denton Desk IV sputter coater (Denton Vacuum, Moorestown, NJ, USA), and imaged on a Zeiss Supra25 field emission scanning electron microscope (Carl Zeiss Microscopy, LLC, Thornwood, NY, USA).\u00a0 At least 15 cells per sample were imaged and the number of coccoliths/cell\u00a0was manually counted to determine the number of coccoliths that remained after the acidification/neutralization dissolution step. 24 h incubation in either light or dark conditions:\u00a0 To determine the number of coccoliths formed (as a proxy for calcification rate) in 24 h in either light or dark conditions, for each\u00a0pCO2\u00a0level, 15 mL of de- lithed culture were added to 8 scintillation vials.\u00a0 Three vials were \u2018light\u2019 replicates, three vials were \u2018dark\u2019 replicates, and two vials were poisoned with buffered formalin to serve as a \u2018light\u2019 blank and a \u2018dark\u2019 blank.\u00a0 Dark replicate and blank vials were covered in black aluminum foil and all vials were incubated together for 24 h in an incubator set at 16.5 +/- 0.5 deg C and 470 umol photons/m-2/s\u00a0PAR on a 14-10 h light-dark cycle.\u00a0The experiment was timed to start when the lights in the incubator turned on in the morning, thus the \u2018light\u2019 replicates were exposed to light for 14 of 24 h.\u00a0 Determination of attached coccoliths:\u00a0\u00a0Coccolith formation was assessed by counting the number of coccoliths formed during the incubation period.\u00a0 After the 24 h incubation period, each replicate and blank vial was filtered onto a 0.4 um polycarbonate filters.\u00a0 Filters were mounted on stubs, sputter-coated with gold using a Denton Desk IV sputter coater (Denton Vacuum, Moorestown, NJ, USA), and imaged on a Zeiss Supra25 field emission SEM (Carl Zeiss Microscopy, LLC, Thornwood, NY, USA).\u00a0 At least 15 cells per replicate were imaged and the number of coccoliths/cell\u00a0was manually counted.\u00a0 The counted coccoliths represented calcification during the 24 h incubation period and the average number of coccoliths per cell for each replicate and blank is reported.
attribute	NC_GLOBAL	awards_0_award_nid	String	514411
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1220068
attribute	NC_GLOBAL	awards_0_data_url	String	http://nsf.gov/awardsearch/showAward?AWD_ID=1220068
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Light-Dark Calcification W. Balch and D. Fields, PIs Version 4 November 2016
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2016-11-04T21:25:51Z
attribute	NC_GLOBAL	date_modified	String	2019-04-18T15:55:03Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.664013.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/664013
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	in-situ incubator
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Dark replicate and blank vials were covered in black aluminum foil and all vials were incubated together for 24 h in an incubator set at 16.5 +/- 0.5 deg C and 470 umol photons/m-2/s PAR on a 14-10 h light-dark cycle.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	664040
attribute	NC_GLOBAL	instruments_0_description	String	A device on shipboard or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/82/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	In-situ incubator
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	494
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Incubator
attribute	NC_GLOBAL	instruments_1_acronym	String	pH Sensor
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Orion ROSS electrode was connected to an Orion Star A211 Benchtop pH meter (ThermoFisher Scientific, Waltham, MA, USA)
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	664025
attribute	NC_GLOBAL	instruments_1_description	String	General term for an instrument that measures the pH or how acidic or basic a solution is.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	pH Sensor
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	674
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Orion ROSS electrode
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Carl Zeiss Microscopy, LLC, Thornwood, NY, USA
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	664039
attribute	NC_GLOBAL	instruments_2_description	String	Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of electrons behaving as waves.
attribute	NC_GLOBAL	instruments_2_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB07/
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Microscope-Electron
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	709
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Zeiss Supra25 field emission SEM
attribute	NC_GLOBAL	instruments_3_acronym	String	MFC
attribute	NC_GLOBAL	instruments_3_dataset_instrument_description	String	Indicate and control set flow rates of gases. Manufactured in Orangeburg, NY USA.
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	664022
attribute	NC_GLOBAL	instruments_3_description	String	Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Mass Flow Controller
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	712
attribute	NC_GLOBAL	instruments_3_supplied_name	String	Aalborg Mass Flow Controller
attribute	NC_GLOBAL	instruments_4_acronym	String	CO2 Adsorber
attribute	NC_GLOBAL	instruments_4_dataset_instrument_description	String	Instrument stripped compressed air of CO2
attribute	NC_GLOBAL	instruments_4_dataset_instrument_nid	String	664023
attribute	NC_GLOBAL	instruments_4_description	String	CO2 Adsorber - an instrument designed to remove CO2 and moisture from compressed air.
attribute	NC_GLOBAL	instruments_4_instrument_name	String	CO2 Adsorber
attribute	NC_GLOBAL	instruments_4_instrument_nid	String	651526
attribute	NC_GLOBAL	instruments_4_supplied_name	String	Puregas VCD CO2 Adsorber
attribute	NC_GLOBAL	instruments_5_acronym	String	Sputter Coater
attribute	NC_GLOBAL	instruments_5_dataset_instrument_description	String	Filters were mounted on stubs, sputter-coated with gold using a Denton Desk IV sputter coater (Denton Vacuum, Moorestown, NJ, USA).
attribute	NC_GLOBAL	instruments_5_dataset_instrument_nid	String	664042
attribute	NC_GLOBAL	instruments_5_description	String	Sputter coating is the standard method for preparing non-conducting or poorly conducting specimens prior to observation in a scanning electron microscope (SEM)
attribute	NC_GLOBAL	instruments_5_instrument_name	String	Sputter Coater
attribute	NC_GLOBAL	instruments_5_instrument_nid	String	664041
attribute	NC_GLOBAL	instruments_5_supplied_name	String	Denton Desk IV sputter coater
attribute	NC_GLOBAL	keywords	String	attached, attached_blankCorrected, bco, bco-dmo, biological, blank, carbon, carbon dioxide, chemical, co2, conditions, corrected, data, dataset, deviation, dioxide, dmo, erddap, light, light_conditions, management, mean, mean_attached_blankCorrected, oceanography, office, pCO2_treatment, preliminary, sample, standard, standard deviation, stdev, stdev_attached_blankCorrected, treatment
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/664013/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/664013
attribute	NC_GLOBAL	param_mapping	String	{'664013': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/664013/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Bigelow Laboratory for Ocean Sciences
attribute	NC_GLOBAL	people_0_person_name	String	William M. Balch
attribute	NC_GLOBAL	people_0_person_nid	String	50650
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Bigelow Laboratory for Ocean Sciences
attribute	NC_GLOBAL	people_1_person_name	String	David Fields
attribute	NC_GLOBAL	people_1_person_nid	String	51141
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Bigelow Laboratory for Ocean Sciences
attribute	NC_GLOBAL	people_2_person_name	String	William M. Balch
attribute	NC_GLOBAL	people_2_person_nid	String	50650
attribute	NC_GLOBAL	people_2_role	String	Contact
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	people_3_affiliation	String	Bigelow Laboratory for Ocean Sciences
attribute	NC_GLOBAL	people_3_person_name	String	Meredith White
attribute	NC_GLOBAL	people_3_person_nid	String	514420
attribute	NC_GLOBAL	people_3_role	String	Contact
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	people_4_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_4_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_4_person_name	String	Hannah Ake
attribute	NC_GLOBAL	people_4_person_nid	String	650173
attribute	NC_GLOBAL	people_4_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_4_role_type	String	related
attribute	NC_GLOBAL	project	String	OA_Copes_Coccoliths
attribute	NC_GLOBAL	projects_0_acronym	String	OA_Copes_Coccoliths
attribute	NC_GLOBAL	projects_0_description	String	(Extracted from the NSF award abstract) Ocean acidification is one of the most pressing marine science issues of our time, with potential biological impacts spanning all marine phyla and potential societal impacts affecting man's relationship to the sea. Rising levels of atmospheric pCO2 are increasing the acidity of the world oceans. It is generally held that average surface ocean pH has already declined by 0.1 pH units relative to the pre-industrial level (Orr et al., 2005), and is projected to decrease 0.3 to 0.46 units by the end of this century, depending on CO2 emission scenarios (Caldeira and Wickett, 2005). The overall goal of this research is to parameterize how changes in pCO2 levels could alter the biological and alkalinity pumps of the world ocean. Specifically, the direct and indirect effects of ocean acidification will be examined within a simple, controlled predator/prey system containing a single prey phytoplankton species (the coccolithophore, Emiliania huxleyi) and a single predator (the oceanic metazoan grazer, Calanus finmarchicus). The experiments are designed to elucidate both direct effects (i.e. effects of ocean acidification on the individual organisms only) and interactive effects (i.e. effects on the combined predator/prey system). Interactive experiments with phytoplankton prey and zooplankton predator are a critical starting point for predicting the overall impact of ocean acidification in marine ecosystems. To meet these goals, a state-of-the-art facility will be constructed with growth chambers that are calibrated and have highly-controlled pH and alkalinity levels. The strength of this approach lies in meticulous calibration and redundant measurements that will be made to ensure that conditions within the chambers are well described and tightly monitored for DIC levels. Growth and calcification rates in coccolithophores and the developmental rates, morphological and behavioral effects on copepods will be measured. The PIC and POC in the algae and the excreted fecal pellets will be monitored for changes in the PIC/POC ratio, a key parameter for modeling feedback mechanisms for rising pCO2 levels. In addition, 14C experiments are planned to measure calcification rates in coccolithophores and dissolution rates as a result of grazing. These key experiments will verify closure in the mass balance of PIC, allowing the determination of actual dissolution rates of PIC within the guts of copepod grazers.
attribute	NC_GLOBAL	projects_0_end_date	String	2015-07
attribute	NC_GLOBAL	projects_0_geolocation	String	Laboratory experiments; East Boothbay, Maine
attribute	NC_GLOBAL	projects_0_name	String	Effects of ocean acidification on Emiliania huxleyi and Calanus finmarchicus; insights into the oceanic alkalinity and biological carbon pumps
attribute	NC_GLOBAL	projects_0_project_nid	String	514415
attribute	NC_GLOBAL	projects_0_start_date	String	2012-08
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Light-dark calcification rates of Pleurochrysis carterae analyzed at Bigelow Laboratory in 2013 (OA Copes Coccoliths project)
attribute	NC_GLOBAL	title	String	Light-dark calcification rates of Pleurochrysis carterae analyzed at Bigelow Laboratory in 2013 (OA Copes Coccoliths project)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	pCO2_treatment		short
attribute	pCO2_treatment	_FillValue	short	32767
attribute	pCO2_treatment	actual_range	short	280, 750
attribute	pCO2_treatment	bcodmo_name	String	treatment
attribute	pCO2_treatment	description	String	The independent variable - one of three pCO2 levels (280 ppm, 380 ppm, or 750 ppm) These treatment levels are nominal values as they represent the target pCO2 for each treatment.
attribute	pCO2_treatment	long_name	String	P CO2 Treatment
attribute	pCO2_treatment	units	String	parts per million (ppm)
variable	sample		String
attribute	sample	bcodmo_name	String	sample
attribute	sample	description	String	For each pCO2 treatment there are three types of samples; After de-lithing: These samples were taken immediately after the coccolithophores were acidified to dissolve their coccoliths and then neutralized to return the culture pH to the original pH. These samples were taken before the 24 h incubation period and therefore do not have a Light or Dark treatment. Replicate: These represent the three replicates for each light condition and each CO2 treatment. These samples were taken after the cells incubated in either light or dark conditions for 24 h. Blank: These samples were killed by the addition of formalin but were allowed to incubate in either light or dark conditions for 24 h.
attribute	sample	long_name	String	Sample
attribute	sample	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	sample	units	String	unitless
variable	light_conditions		String
attribute	light_conditions	bcodmo_name	String	PAR
attribute	light_conditions	description	String	This identifies whether the sample was incubated for 24 h in light (470 umol photons/m-2/s PAR) or dark (0 umol photons/m-2/s PAR) conditions.
attribute	light_conditions	long_name	String	Light Conditions
attribute	light_conditions	units	String	unitless
variable	attached		float
attribute	attached	_FillValue	float	NaN
attribute	attached	actual_range	float	0.0, 33.0
attribute	attached	bcodmo_name	String	cell_concentration
attribute	attached	description	String	This is the number of attached coccoliths observed per cell. This number represents an average of counts from at least 15 cells per replicate or sample.
attribute	attached	long_name	String	Attached
attribute	attached	units	String	coccoliths per cell
variable	attached_blankCorrected		float
attribute	attached_blankCorrected	_FillValue	float	NaN
attribute	attached_blankCorrected	actual_range	float	0.0, 32.8
attribute	attached_blankCorrected	bcodmo_name	String	cell_concentration
attribute	attached_blankCorrected	description	String	This is the number of attached coccoliths observed per cell with the number of coccoliths observed per cell from the blank samples subtracted to account for any coccoliths that might have remained after cells were de-lithed.
attribute	attached_blankCorrected	long_name	String	Attached Blank Corrected
attribute	attached_blankCorrected	units	String	coccoliths per cell
variable	mean_attached_blankCorrected		float
attribute	mean_attached_blankCorrected	_FillValue	float	NaN
attribute	mean_attached_blankCorrected	actual_range	float	0.21, 30.72
attribute	mean_attached_blankCorrected	bcodmo_name	String	mean
attribute	mean_attached_blankCorrected	description	String	The average value for each set of three replicates.
attribute	mean_attached_blankCorrected	long_name	String	Mean Attached Blank Corrected
attribute	mean_attached_blankCorrected	units	String	coccoliths per cell
variable	stdev_attached_blankCorrected		float
attribute	stdev_attached_blankCorrected	_FillValue	float	NaN
attribute	stdev_attached_blankCorrected	actual_range	float	0.37, 2.75
attribute	stdev_attached_blankCorrected	bcodmo_name	String	standard deviation
attribute	stdev_attached_blankCorrected	description	String	The standard deviation for each set of three replicates.
attribute	stdev_attached_blankCorrected	long_name	String	Stdev Attached Blank Corrected
attribute	stdev_attached_blankCorrected	units	String	coccoliths per cell

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.