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     data   graph     files  public [Salp sample log] - Salp specimen log for genomic and transcriptomic study collected from
ARSV Laurence M. Gould, Umitaka-Maru, R/V Polarstern LMG1110, UM-08-09, ANT-XXVII-2 in the
Southern Ocean from 2009-2011 (Salp_Antarctic project) (Population ecology of Salpa thompsoni
based on molecular indicators)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String 1) Cruise R/V LM GOULD (LMG1110): Samples collected using Multiple
Opening/Closing Net and Environmental Sensing System (MOCNESS) with a mouth
opening of 1-m2 and nine 335\u03bcm mesh nets; upper 200 m were sampled with a
2.3 m2 Isaacs-Kidd Midwater Trawl (IKMT) with a 505 \u00b5m mesh
net.\u00a0Western Antarctic Peninsula region, Southern Ocean (November-
December 2011)

2) Cruise\u00a0R/V Polarstern (PS-ANT-XVII/2): Samples collected using a
Rectangular Midwater Trawl (RMT 1+8) from the upper 200 m.\u00a0Western
Antarctic Peninsula region, Southern Ocean (January 2011)

3) Cruise R/V Umitaka Maru (UM-08-09): Samples collected using a RMT 1+8 from
2,000 m to surface. Indian Sector, Southern Ocean (January 2009)
attribute NC_GLOBAL awards_0_award_nid String 54892
attribute NC_GLOBAL awards_0_award_number String ANT-1044982
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1044982 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Antarctic Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF ANT
attribute NC_GLOBAL awards_0_funding_source_nid String 369
attribute NC_GLOBAL awards_0_program_manager String Dr Peter Milne
attribute NC_GLOBAL awards_0_program_manager_nid String 51468
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Salpa thompsoni collection information
PI: A. Bucklin (UConn)
version: 2017-01-06
NOTE: These data were published in Table 1, Batta-Lona et al (2016) Pol. Bio. doi:10.1007/s00300-016-2051-6
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2017-01-06T19:21:21Z
attribute NC_GLOBAL date_modified String 2018-10-03T18:16:40Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.728220
attribute NC_GLOBAL Easternmost_Easting double 42.002
attribute NC_GLOBAL geospatial_lat_max double -60.901
attribute NC_GLOBAL geospatial_lat_min double -66.183
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double 42.002
attribute NC_GLOBAL geospatial_lon_min double -74.567
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/672600 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String MOC1
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 674089
attribute NC_GLOBAL instruments_0_description String The Multiple Opening/Closing Net and Environmental Sensing System or MOCNESS is a family of net systems based on the Tucker Trawl principle. The MOCNESS-1 carries nine 1-m2 nets usually of 335 micrometer mesh and is intended for use with the macrozooplankton. All nets are black to reduce contrast with the background. A motor/toggle release assembly is mounted on the top portion of the frame and stainless steel cables with swaged fittings are used to attach the net bar to the toggle release. A stepping motor in a pressure compensated case filled with oil turns the escapement crankshaft of the toggle release which sequentially releases the nets to an open then closed position on command from the surface. -- from the MOCNESS Operations Manual (1999 + 2003).
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L22/current/NETT0097/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String MOCNESS1
attribute NC_GLOBAL instruments_0_instrument_nid String 437
attribute NC_GLOBAL instruments_0_supplied_name String Multiple Opening/Closing Net and Environmental Sensing System (MOCNESS) with a mouth opening of 1-m2 and nine 335μm mesh nets
attribute NC_GLOBAL instruments_1_acronym String TrawlMid
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 674090
attribute NC_GLOBAL instruments_1_description String A mid-water or pelagic trawl is a net towed at a chosen depth in the water column to catch schooling fish such as herring and mackerel. Midwater trawl nets have very large front openings to herd schooling fish toward the back end where they become trapped in the narrow "broiler". The sides of the deployed net are spread horizontally with two large metal foils, called "doors," positioned in front of the net. As the trawler moves forward, the doors, and therefore the net, are forced outward, keeping the net open.
This instrument designation is used when specific make and model are not known.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/23/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Midwater Trawl
attribute NC_GLOBAL instruments_1_instrument_nid String 467
attribute NC_GLOBAL instruments_1_supplied_name String Rectangular Midwater Trawl (RMT 1+8)
attribute NC_GLOBAL instruments_2_acronym String IKMT
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 674091
attribute NC_GLOBAL instruments_2_description String A trawl with a pentagonal mouth opening and a dihedral depressor vane as part of the mouth opening. IKMTs come in various dimensions (refer to individual dataset documentation).

The original IKMTs were 10 foot (304 cm) and 15 foot (457 cm) at the mouth. The 10 foot IKMT net was 31 feet (9.45 m) in length (Wiebe and Benfield 2003).
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L22/current/NETT0071/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Isaacs-Kidd Midwater Trawl
attribute NC_GLOBAL instruments_2_instrument_nid String 715
attribute NC_GLOBAL instruments_2_supplied_name String 2.3 m2 Isaacs-Kidd Midwater Trawl (IKMT) with a 505 µm mesh net
attribute NC_GLOBAL keywords String altimetry, area, bco, bco-dmo, biological, chemical, cruise, cruise_id, data, dataset, depth, depth_range, depth_w, dmo, erddap, laboratory, latitude, longitude, management, num, num_samples, oceanography, office, preliminary, region, sal, samples, satellite, season, station, temperature
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/672600/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/672600 (external link)
attribute NC_GLOBAL Northernmost_Northing double -60.901
attribute NC_GLOBAL param_mapping String {'672600': {'lat': 'master - latitude', 'lon': 'master - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/672600/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Connecticut
attribute NC_GLOBAL people_0_affiliation_acronym String UConn - Avery Point
attribute NC_GLOBAL people_0_person_name String Ann Bucklin
attribute NC_GLOBAL people_0_person_nid String 50389
attribute NC_GLOBAL people_0_role String Chief Scientist
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of Connecticut
attribute NC_GLOBAL people_1_affiliation_acronym String UConn - Avery Point
attribute NC_GLOBAL people_1_person_name String Ann Bucklin
attribute NC_GLOBAL people_1_person_nid String 50389
attribute NC_GLOBAL people_1_role String Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of Connecticut
attribute NC_GLOBAL people_2_affiliation_acronym String UConn
attribute NC_GLOBAL people_2_person_name String Dr Rachel J. O'Neill
attribute NC_GLOBAL people_2_person_nid String 51393
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Connecticut Sea Grant
attribute NC_GLOBAL people_3_affiliation_acronym String CTSG
attribute NC_GLOBAL people_3_person_name String Dr Diana Payne
attribute NC_GLOBAL people_3_person_nid String 51392
attribute NC_GLOBAL people_3_role String Co-Principal Investigator
attribute NC_GLOBAL people_3_role_type String originator
attribute NC_GLOBAL people_4_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_4_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_4_person_name String Nancy Copley
attribute NC_GLOBAL people_4_person_nid String 50396
attribute NC_GLOBAL people_4_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_4_role_type String related
attribute NC_GLOBAL project String Salp_Antarctic
attribute NC_GLOBAL projects_0_acronym String Salp_Antarctic
attribute NC_GLOBAL projects_0_description String The Antarctic salp, Salpa thompsoni, is an increasingly important player in the vulnerable Antarctic Peninsula pelagic ecosystem. Observations of high abundance of Salpa thompsoni during the summer in the Southern Ocean suggest that this species is capable of rapid somatic and population growth, and frequently forms dense blooms under favorable environmental conditions. The proposed research will examine genome-wide patterns of gene expression, target gene expression levels, and patterns of population genetic diversity and structure of the target salp species. Our preliminary results and data analysis have provided a promising basis for transcriptomic studies of S. thompsoni in the Southern Ocean. The proposed next steps in our genomic/transcriptomic analysis of Salpa thompsoni are: 1) completion of a reference transcriptome as a basis for genome-wide analysis of gene expression; 2) whole transcriptome shotgun sequencing (RNA-Seq) analysis to characterize gene expression in relation to individual characteristics and environmental conditions; 3) quantitative real-time PCR (qRT-PCR) characterization and validation of gene expression for 10-20 top differentially-expressed genes; and 4) detection of strand-specific allelic variation at SNP (Single Nucleotide Polymorphic) sites to analyze clonal diversity and population genetic diversity and structure. We hypothesize that: 1) deep analysis of the Salpa thompsoni transcriptome will reveal significant associations among selected set of differentially-expressed genes and critical life history stages and events (e.g., ontogenetic maturation, sexual reproduction, senescence) of the salp; and 2) the species will show variable levels of clonal diversity and significant genetic differentiation among salp populations in different regions of the Southern Ocean. Samples will be obtained from research cruises during 2011-2013 in diverse regions of the Southern Ocean; dedicated sample and data collection will be carried out during a cruise of the R/V LM GOULD (LMG11-10) to the Western Antarctic Peninsula region in November, 2011. The significance of this effort lies in new understanding of the molecular processes underlying the complex life history and population dynamics of S. thompsoni in relation to the Antarctic pelagic ecosystem and extreme and variable environmental conditions of the Southern Ocean.
Most of the data from this project are available from the Marine Geoscience Data System (MGDS), part of IEDA and is available at https://www.marine-geo.org/tools/search/Files.php?data_set_uid=18148.
attribute NC_GLOBAL projects_0_end_date String 2014-05
attribute NC_GLOBAL projects_0_geolocation String Southern Ocean
attribute NC_GLOBAL projects_0_name String Population ecology of Salpa thompsoni based on molecular indicators
attribute NC_GLOBAL projects_0_project_nid String 2174
attribute NC_GLOBAL projects_0_start_date String 2011-06
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double -66.183
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String This dataset is a log of the Salpa thompsoni specimens used for
genomics/transcriptomics. Reported parameters include cruise id, region,
season and area, location, number of samples collected, temperature, salinity,
depth range of the samples, and the depth of the water at the collection site.

Related Dataset: [Salp genome and transcriptome](\\https://www.bco-
dmo.org/dataset/675040\\)
attribute NC_GLOBAL title String [Salp sample log] - Salp specimen log for genomic and transcriptomic study collected from ARSV Laurence M. Gould, Umitaka-Maru, R/V Polarstern LMG1110, UM-08-09, ANT-XXVII-2 in the Southern Ocean from 2009-2011 (Salp_Antarctic project) (Population ecology of Salpa thompsoni based on molecular indicators)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -74.567
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable cruise_id   String  
attribute cruise_id bcodmo_name String cruise_id
attribute cruise_id description String cruise identifier
attribute cruise_id long_name String Cruise Id
attribute cruise_id units String unitless
variable region   String  
attribute region bcodmo_name String region
attribute region description String region of Southern Ocean
attribute region long_name String Region
attribute region units String unitless
variable season   String  
attribute season bcodmo_name String season
attribute season description String season and year of collection
attribute season long_name String Season
attribute season units String unitless
variable area   String  
attribute area bcodmo_name String region
attribute area description String area within region of Southern Ocean
attribute area long_name String Area
attribute area units String unitless
variable station   short  
attribute station _FillValue short 32767
attribute station actual_range short 4, 184
attribute station bcodmo_name String station
attribute station description String station number
attribute station long_name String Station
attribute station units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double -66.183, -60.901
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String latitude; north is positive
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -74.567, 42.002
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String longitude; east is positive
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable num_samples   byte  
attribute num_samples _FillValue byte 127
attribute num_samples actual_range byte 4, 5
attribute num_samples bcodmo_name String count
attribute num_samples description String number of samples collected
attribute num_samples long_name String Num Samples
attribute num_samples units String unitless
variable temp   float  
attribute temp _FillValue float NaN
attribute temp actual_range float -1.28, 1.28
attribute temp bcodmo_name String temperature
attribute temp description String temperature
attribute temp long_name String Temperature
attribute temp nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/ (external link)
attribute temp units String degrees Celsius
variable sal   float  
attribute sal _FillValue float NaN
attribute sal actual_range float 33.39, 34.6
attribute sal bcodmo_name String sal
attribute sal description String salinity
attribute sal long_name String Sal
attribute sal nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PSALST01/ (external link)
attribute sal units String PPT
variable depth_range   String  
attribute depth_range bcodmo_name String depth_range
attribute depth_range description String sampled depth
attribute depth_range long_name String Depth
attribute depth_range standard_name String depth
attribute depth_range units String meters
variable depth_w   short  
attribute depth_w _FillValue short 32767
attribute depth_w actual_range short 452, 4300
attribute depth_w bcodmo_name String depth_w
attribute depth_w colorBarMaximum double 8000.0
attribute depth_w colorBarMinimum double -8000.0
attribute depth_w colorBarPalette String TopographyDepth
attribute depth_w description String water column depth
attribute depth_w long_name String Depth
attribute depth_w standard_name String depth
attribute depth_w units String meters

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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