ERDDAP > info > bcodmo_dataset_680935

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
	set	data	graph		files	public	[Quantitative PCR data] - Quantitative PCR data from samples collected on the R/V Sarmiento de Gamboa cruise in the subtropical North Atlantic Ocean between January and March 2011 (Microbial associations in zooplankton project) (Microbial associations in zooplankton: significance for the marine nitrogen cycle)		F   I   M	background			BCO-DMO	bcodmo_dataset_680935

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson,.odvTxt
attribute	NC_GLOBAL	acquisition_description	String	Samples were collected from Niskin bottles, filtered, and processed for DNA analyses. For detailed methods see Benavides et al. 2016.
attribute	NC_GLOBAL	awards_0_award_nid	String	542156
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1130495
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward?AWD_ID=1130495
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	qPCR Data Moisander and Benavidas, PIs Version 2 February 2017
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2017-02-02T20:50:59Z
attribute	NC_GLOBAL	date_modified	String	2019-04-04T18:19:43Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.680935.1
attribute	NC_GLOBAL	Easternmost_Easting	double	-13.3
attribute	NC_GLOBAL	geospatial_lat_max	double	27.8
attribute	NC_GLOBAL	geospatial_lat_min	double	24.5
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	-13.3
attribute	NC_GLOBAL	geospatial_lon_min	double	-76.2
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/680935
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Thermal Cycler
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used to process PCR samples
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	680992
attribute	NC_GLOBAL	instruments_0_description	String	General term for a laboratory apparatus commonly used for performing polymerase chain reaction (PCR). The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. (adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)
attribute	NC_GLOBAL	instruments_0_instrument_name	String	PCR Thermal Cycler
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	471582
attribute	NC_GLOBAL	instruments_0_supplied_name	String	PCR
attribute	NC_GLOBAL	keywords	String	a11, bco, bco-dmo, biological, chemical, data, dataset, date, dmo, erddap, fraction, gamma, Gamma_24774A11, group, group_B, het1, iso, latitude, longitude, management, number, oceanography, office, preliminary, project, size, size_fraction, station, time, time2, tricho, ucyn, UCYN_A
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/680935/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/680935
attribute	NC_GLOBAL	Northernmost_Northing	double	27.8
attribute	NC_GLOBAL	param_mapping	String	{'680935': {'lat': 'master - latitude', 'lon': 'master - longitude', 'ISO_DateTime_UTC': 'master - time'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/680935/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of Massachusetts Dartmouth
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UMASSD-SMAST
attribute	NC_GLOBAL	people_0_person_name	String	Dr Pia Moisander
attribute	NC_GLOBAL	people_0_person_nid	String	564454
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Université de Toulon
attribute	NC_GLOBAL	people_1_person_name	String	Mar Benavides
attribute	NC_GLOBAL	people_1_person_nid	String	680979
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_2_person_name	String	Hannah Ake
attribute	NC_GLOBAL	people_2_person_nid	String	650173
attribute	NC_GLOBAL	people_2_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	project	String	Microbial associations in zooplankton
attribute	NC_GLOBAL	projects_0_acronym	String	Microbial associations in zooplankton
attribute	NC_GLOBAL	projects_0_description	String	Description from NSF award abstract: Nitrogen (N2) fixation is a key biogeochemical process providing new N for the marine ecosystem. Current estimates of the global rates suggest that the inputs and outputs of N are not in balance, leaving an apparent deficiency in N2 fixation in the oceanic N budget. Based on recovery of N2 fixation gene fragments, however, numerous potential N2-fixing microbes are present in the euphotic layer and below, yet evidence for their contribution to N2-fixation is lacking. The goal of this study is to identify and determine the importance of potentially diazotrophic, heterotrophic prokaryotes occupying a previously unstudied niche in the upper ocean. This project PIs hypothesize that N2 fixation in zooplankton-associated microbial communities is an important source of new N in the open ocean contributing to the oceanic N budget. In oligotrophic, N-limited open ocean waters, zooplankton are faced with the same low N availability in their diets that limits growth of primary producers and heterotrophic microbes in the open ocean. Zooplankton guts would be a particularly suitable environment for heterotrophic N2-fixers as the nitrogenase enzyme is inhibited by oxygen. In many terrestrial insects, N2 fixation by gut microbes serves as a source of N. The aim of this study is to investigate whether similar associations occur in marine zooplankton that live on N-limited phytoplankton. The PIs will investigate specific associations between copepods from N-limited and non-N limited oceanic waters with microorganisms that are potentially or actively fixing N. They will also investigate the fate of this N to study whether the new N is incorporated into the zooplankton host. The main research objectives are to determine whether: 1. diazotrophic microflora exist in copepods and what is their seasonal and spatial variability, 2. microbial communities associated with copepods are actively fixing N2, 3. diazotroph communities contribute to the N nutrition of their zooplankton hosts. Monthly samples will be collected in the oligotrophic Sargasso Sea, in association with BATS sampling cruises. In addition, experiments and field studies will be conducted in Bermuda during the summer. For comparison to a site that is not severely N limited, and for methods development, additional studies will be carried out in coastal Gulf of Maine waters. Selected copepod species and their fecal pellets will be analyzed for the presence of nifH genes and their expression using cloning and sequencing and Reverse Transcriptase-PCR. Quantitative PCR will be employed to investigate the seasonal distributions of dominant phylotypes. Anaerobic cultivation will be used for the enrichment of the gut microflora. Zooplankton will be incubated with 15N2 to quantify microbial N2 fixation activity and its fate of N through zooplankton overall 15N content and nanoSIMS determination of 15N localization. Products of this study will include novel microbial isolates or consortia, evidence regarding taxa-specific expression of N2 fixation in these microhabitats, and a first estimate for the importance of this process for the oceanic N budget. This project addresses fundamental issues regarding the sources of oceanic nitrogen and will test hypotheses regarding previously overlooked diazotrophic niches. As open ocean productivity is frequently N limited, characterization of new sources of N will have global impacts.
attribute	NC_GLOBAL	projects_0_end_date	String	2016-08
attribute	NC_GLOBAL	projects_0_geolocation	String	Sargasso Sea, Eastern and Western North Atlantic.
attribute	NC_GLOBAL	projects_0_name	String	Microbial associations in zooplankton: significance for the marine nitrogen cycle
attribute	NC_GLOBAL	projects_0_project_nid	String	564455
attribute	NC_GLOBAL	projects_0_start_date	String	2011-09
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	24.5
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	subsetVariables	String	project
attribute	NC_GLOBAL	summary	String	Quantitative PCR data from samples collected on the R/V Sarmiento de Gamboa cruise in the subtropical North Atlantic Ocean between January and March 2011 (Microbial associations in zooplankton project)
attribute	NC_GLOBAL	time_coverage_end	String	2011-03-09T15:10:00Z
attribute	NC_GLOBAL	time_coverage_start	String	2011-01-28T08:25:00Z
attribute	NC_GLOBAL	title	String	[Quantitative PCR data] - Quantitative PCR data from samples collected on the R/V Sarmiento de Gamboa cruise in the subtropical North Atlantic Ocean between January and March 2011 (Microbial associations in zooplankton project) (Microbial associations in zooplankton: significance for the marine nitrogen cycle)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	Westernmost_Easting	double	-76.2
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	project		String
attribute	project	bcodmo_name	String	project
attribute	project	description	String	PI issued project name
attribute	project	long_name	String	Project
attribute	project	units	String	unitless
variable	date		String
attribute	date	bcodmo_name	String	date
attribute	date	description	String	Date of sampling; YYYY/mm/dd
attribute	date	long_name	String	Date
attribute	date	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	date	time_precision	String	1970-01-01
attribute	date	units	String	unitless
variable	time2		String
attribute	time2	bcodmo_name	String	time
attribute	time2	description	String	Time of sampling; HH:MM
attribute	time2	long_name	String	Time
attribute	time2	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/AHMSAA01/
attribute	time2	units	String	unitless
variable	station		short
attribute	station	_FillValue	short	32767
attribute	station	actual_range	short	1, 139
attribute	station	bcodmo_name	String	station
attribute	station	description	String	Station where sampling occurred
attribute	station	long_name	String	Station
attribute	station	units	String	unitless
variable	latitude		double
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	_FillValue	double	NaN
attribute	latitude	actual_range	double	24.5, 27.8
attribute	latitude	axis	String	Y
attribute	latitude	bcodmo_name	String	latitude
attribute	latitude	colorBarMaximum	double	90.0
attribute	latitude	colorBarMinimum	double	-90.0
attribute	latitude	description	String	Latitude; N is positive
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LATX/
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	longitude		double
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	_FillValue	double	NaN
attribute	longitude	actual_range	double	-76.2, -13.3
attribute	longitude	axis	String	X
attribute	longitude	bcodmo_name	String	longitude
attribute	longitude	colorBarMaximum	double	180.0
attribute	longitude	colorBarMinimum	double	-180.0
attribute	longitude	description	String	Longitude; E is positive
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LONX/
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east
variable	number		short
attribute	number	_FillValue	short	32767
attribute	number	actual_range	short	5691, 5735
attribute	number	bcodmo_name	String	sample
attribute	number	colorBarMaximum	double	100.0
attribute	number	colorBarMinimum	double	0.0
attribute	number	description	String	Sample ID number
attribute	number	long_name	String	Number
attribute	number	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	number	units	String	unitless
variable	size_fraction		float
attribute	size_fraction	_FillValue	float	NaN
attribute	size_fraction	actual_range	float	0.2, 10.0
attribute	size_fraction	bcodmo_name	String	filter_size
attribute	size_fraction	colorBarMaximum	double	1.0
attribute	size_fraction	colorBarMinimum	double	0.0
attribute	size_fraction	description	String	Filter size
attribute	size_fraction	long_name	String	Size Fraction
attribute	size_fraction	units	String	microns
variable	UCYN_A		float
attribute	UCYN_A	_FillValue	float	NaN
attribute	UCYN_A	actual_range	float	0.0, 198531.3
attribute	UCYN_A	bcodmo_name	String	genetic_conc
attribute	UCYN_A	description	String	UCYN-A gene copy concentration
attribute	UCYN_A	long_name	String	UCYN A
attribute	UCYN_A	units	String	genes per liter
variable	Gamma_24774A11		float
attribute	Gamma_24774A11	_FillValue	float	NaN
attribute	Gamma_24774A11	actual_range	float	0.0, 3320.6
attribute	Gamma_24774A11	bcodmo_name	String	genetic_conc
attribute	Gamma_24774A11	description	String	Gamma 24774A11 gene copy concentration
attribute	Gamma_24774A11	long_name	String	Gamma 24774 A11
attribute	Gamma_24774A11	units	String	genes per liter
variable	group_B		float
attribute	group_B	_FillValue	float	NaN
attribute	group_B	actual_range	float	0.0, 1934.5
attribute	group_B	bcodmo_name	String	genetic_conc
attribute	group_B	description	String	Group B gene copy concentration
attribute	group_B	long_name	String	Group B
attribute	group_B	units	String	genes per liter
variable	Tricho		float
attribute	Tricho	_FillValue	float	NaN
attribute	Tricho	actual_range	float	0.0, 875890.7
attribute	Tricho	bcodmo_name	String	genetic_conc
attribute	Tricho	description	String	Tricho gene copy concentration
attribute	Tricho	long_name	String	Tricho
attribute	Tricho	units	String	genes per liter
variable	Het1		float
attribute	Het1	_FillValue	float	NaN
attribute	Het1	actual_range	float	0.0, 342.5
attribute	Het1	bcodmo_name	String	genetic_conc
attribute	Het1	description	String	Het1 gene copy concentration
attribute	Het1	long_name	String	Het1
attribute	Het1	units	String	genes per liter
variable	time		double
attribute	time	_CoordinateAxisType	String	Time
attribute	time	actual_range	double	1.2962031E9, 1.2996834E9
attribute	time	axis	String	T
attribute	time	bcodmo_name	String	ISO_DateTime_UTC
attribute	time	description	String	Date/Time (UTC) ISO formatted
attribute	time	ioos_category	String	Time
attribute	time	long_name	String	ISO Date Time UTC
attribute	time	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/
attribute	time	source_name	String	ISO_DateTime_UTC
attribute	time	standard_name	String	time
attribute	time	time_origin	String	01-JAN-1970 00:00:00
attribute	time	time_precision	String	1970-01-01T00:00:00Z
attribute	time	units	String	seconds since 1970-01-01T00:00:00Z

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.