ERDDAP > info > bcodmo_dataset_682298

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Seeded microcosm experiments: all data] - Nutrients, microbiology, trace metals, and environmental conditions from seeded microcosm experiments (Vibrio as a model microbe for opportunistic heterotrophic response to Saharan dust deposition events in marine waters)		I   M	background			BCO-DMO	bcodmo_dataset_682298

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Samples were collected offshore from Alligator Reef (2014) and Looe Key Reef (2015). The experimental treatments included additions of dust and iron in 2014 with a control of acidified water. In 2015, treatments included additions of dust from Barbados, iron, nitrates, phosphates, and carbon with controls of acidified water and leachate. Nutrients data went through internal lab QAQC process. BDL means below detection limit. \u00a0The method detection limit (MDL) was determined using 9 samples on two different runs and correct student-T value.
attribute	NC_GLOBAL	awards_0_award_nid	String	553932
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1357423
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1357423
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Michael E. Sieracki
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50446
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Seeded microcosm experiments Nutrients, microbiology, trace metals, and environmental conditions PI's: E. Lipp (UGA), W. Landing (FSU) E. Ottesen (UGA), M. Wetz (TAMU) version: 2016-11-01 BDL = below detection limit
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	dataset_current_state	String	Final with updates expected
attribute	NC_GLOBAL	date_created	String	2017-02-15T15:51:25Z
attribute	NC_GLOBAL	date_modified	String	2020-05-18T19:24:17Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.682298.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/682298
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Gas Analyzer
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	To measure dissolved organic nitrogen
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	682308
attribute	NC_GLOBAL	instruments_0_description	String	Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Gas Analyzer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	720
attribute	NC_GLOBAL	instruments_0_supplied_name	String	chemoluminescence gas analyzer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	To measure colony counts
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	682307
attribute	NC_GLOBAL	instruments_1_description	String	Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: https://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	plate reader
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	528693
attribute	NC_GLOBAL	keywords	String	ammonium, bco, bco-dmo, biological, chemical, chemistry, Chl_a, chlorophyll, commerce, concentration, concentration_of_chlorophyll_in_sea_water, dark, data, dataset, department, dFe, dmo, doc, don, earth, Earth Science > Oceans > Ocean Chemistry > Chlorophyll, elapsed, erddap, expt, light, light_dark, management, mole, mole_concentration_of_nitrite_in_sea_water, nh4, nitrate, nitrite, NO2, no3, ocean, oceanography, oceans, office, orthophosphate, preliminary, replicate, sample, science, sea, seawater, SiO4, time, time_elapsed_hr, treatment, vessel, vibrio, water, year
attribute	NC_GLOBAL	keywords_vocabulary	String	GCMD Science Keywords
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/682298/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/682298
attribute	NC_GLOBAL	param_mapping	String	{'682298': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/682298/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of Georgia
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UGA
attribute	NC_GLOBAL	people_0_person_name	String	Erin K. Lipp
attribute	NC_GLOBAL	people_0_person_nid	String	553935
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Florida State University
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	FSU - EOAS
attribute	NC_GLOBAL	people_1_person_name	String	William M. Landing
attribute	NC_GLOBAL	people_1_person_nid	String	51302
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	University of Georgia
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	UGA
attribute	NC_GLOBAL	people_2_person_name	String	Elizabeth Ottesen
attribute	NC_GLOBAL	people_2_person_nid	String	553937
attribute	NC_GLOBAL	people_2_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	Texas A&M University
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	TAMU
attribute	NC_GLOBAL	people_3_person_name	String	Michael Wetz
attribute	NC_GLOBAL	people_3_person_nid	String	553945
attribute	NC_GLOBAL	people_3_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_3_role_type	String	originator
attribute	NC_GLOBAL	people_4_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_4_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_4_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_4_person_nid	String	50396
attribute	NC_GLOBAL	people_4_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_4_role_type	String	related
attribute	NC_GLOBAL	project	String	Vibrio-dust deposition
attribute	NC_GLOBAL	projects_0_acronym	String	Vibrio-dust deposition
attribute	NC_GLOBAL	projects_0_description	String	Description from NSF award abstract: Dust and mineral aerosols are a significant source of micro and macronutrients to oligotrophic ocean surface waters. Evidence is growing that heterotrophic microbes may play key roles in processing deposited minerals and nutrients. Yet it is not known which components of dust stimulate the heterotrophic bacteria, which cellular mechanisms are responsible for the utilization of those components and how the activity of these bacteria affect the availability and utilization of dust-derived minerals and nutrients by marine autotrophs. Knowledge of these factors is key to understanding how dust deposition impacts carbon cycles and for predicting the response of tropical oceans to future changes in the frequency and intensity of dust deposition events. The objective of this project is to examine the specific effects of aeolian dust on heterotrophic microbes in a tropical marine system under controlled conditions. The central hypothesis is that in oligotrophic tropical systems numerically minor opportunistic bacteria are the first responders to influx of dust constituents and respond primarily by rapidly accessing soluble trace metals and limiting nutrients that are deposited with Saharan dust. The project will focus on two specific aims: 1) Quantify changes in community structure, composition and transcriptional activity among marine microbial populations upon exposure to dust, and 2) Identify key components in Saharan dust aerosols that stimulate or repress growth and/or activity in Vibrio, a model opportunistic marine heterotrophic group. The study will use a series of controlled experiments designed to identify and quantify heterotrophic microbial response to dust deposition events using both natural communities and model bacteria (Vibrio) through metagenomics, transcriptomics and atmospheric and marine biogeochemical techniques. This innovative approach will identify the most critical (reactive) components leached from dust aerosols on the microbial community as well as elucidate potential mechanisms of response. There is great interest in the biological response to dust aerosols given its potentially large influence on biogeochemical cycling, but there has been relatively little work that has addressed the mechanisms of response (especially among the heterotrophic microbial fraction) or identified the relative importance of specific constituents of dust aerosols. A detailed framework for microbial response (focusing on opportunistic heterotrophs) will facilitate efforts to link autotrophic and heterotrophic processing. This contribution is significant because it will provide one of the first end-to-end (chemistry to physiology to ecology) mechanistic pathways for marine biological response to desert dust aerosols.
attribute	NC_GLOBAL	projects_0_end_date	String	2017-03
attribute	NC_GLOBAL	projects_0_geolocation	String	Florida Keys, FL, USA
attribute	NC_GLOBAL	projects_0_name	String	Vibrio as a model microbe for opportunistic heterotrophic response to Saharan dust deposition events in marine waters
attribute	NC_GLOBAL	projects_0_project_nid	String	553933
attribute	NC_GLOBAL	projects_0_start_date	String	2014-04
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Nutrients, microbiology, trace metals, and environmental conditions from seeded microcosm experiments.
attribute	NC_GLOBAL	title	String	[Seeded microcosm experiments: all data] - Nutrients, microbiology, trace metals, and environmental conditions from seeded microcosm experiments (Vibrio as a model microbe for opportunistic heterotrophic response to Saharan dust deposition events in marine waters)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.5
variable	year		short
attribute	year	_FillValue	short	32767
attribute	year	actual_range	short	2014, 2015
attribute	year	bcodmo_name	String	year
attribute	year	description	String	year of measurement
attribute	year	long_name	String	Year
attribute	year	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/YEARXXXX/
attribute	year	units	String	year
variable	expt		String
attribute	expt	bcodmo_name	String	exp_id
attribute	expt	description	String	experiment identifier
attribute	expt	long_name	String	Expt
attribute	expt	units	String	unitless
variable	treatment		String
attribute	treatment	bcodmo_name	String	treatment
attribute	treatment	description	String	experimental treatments: For 2014: control acidified water; dust= dust leachate from Whatman41 filters used to collect dust aerosols from Barbados (added to provide +1 nM Fe); FeCl = FeCl3 (added to provide +1 nM Fe) For 2015:  control acidified water; 2nMFe: 2 nM Fe provided by FeCl3 20nMFe: 20 nM Fe provided by FeCl3 control_leachate: control leachate (leached from Whatman41 blank filter); dust_barbados: dust leachate from Whatman41 filters used to collect dust aerosols from Barbados (added to provide +2 nM Fe); Fe: FeCl3 (added to provide +1 nM Fe); NO3: HNO3 added to provide +0.2 uM N; PO4: K2HPO4 added to provide +0.01 uM P; C: Pyruvate added to provide +10 uM C; FeCNP: mix of FeCl3 (+2 nM Fe), pyruvate (+10 uM C), HNO3 (+0.2 uM), and K2HPO4 (+0.01 uM)
attribute	treatment	long_name	String	Treatment
attribute	treatment	units	String	unitless
variable	light_dark		String
attribute	light_dark	bcodmo_name	String	treatment
attribute	light_dark	description	String	whether experiment took place in the light or dark
attribute	light_dark	long_name	String	Light Dark
attribute	light_dark	units	String	unitless
variable	vessel		byte
attribute	vessel	_FillValue	byte	127
attribute	vessel	actual_range	byte	1, 42
attribute	vessel	bcodmo_name	String	sample
attribute	vessel	description	String	sample vessel identifier
attribute	vessel	long_name	String	Vessel
attribute	vessel	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	vessel	units	String	unitless
variable	replicate		String
attribute	replicate	bcodmo_name	String	replicate
attribute	replicate	description	String	replicate identifier
attribute	replicate	long_name	String	Replicate
attribute	replicate	units	String	unitless
variable	time_elapsed_hr		byte
attribute	time_elapsed_hr	_FillValue	byte	127
attribute	time_elapsed_hr	actual_range	byte	0, 94
attribute	time_elapsed_hr	bcodmo_name	String	time_elapsed
attribute	time_elapsed_hr	description	String	time since start of experiment
attribute	time_elapsed_hr	long_name	String	Time Elapsed Hr
attribute	time_elapsed_hr	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/
attribute	time_elapsed_hr	units	String	hours
variable	sample		String
attribute	sample	bcodmo_name	String	sample
attribute	sample	description	String	sample identifier: 'year_treatment_light or dark_vessel number_replicate_time
attribute	sample	long_name	String	Sample
attribute	sample	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	sample	units	String	unitless
variable	Vibrio		float
attribute	Vibrio	_FillValue	float	NaN
attribute	Vibrio	actual_range	float	0.0, 2800.0
attribute	Vibrio	bcodmo_name	String	unknown
attribute	Vibrio	description	String	Vibrio concentration: determined by spread plating on TCBS agar and countng ager 18-24 incubation at 30 C. Limit of detection was 3.3 CFU/ml (determined using 100 ul spread volume in triplicate). The data uses a value of 0.0 for below detection limit.
attribute	Vibrio	long_name	String	Vibrio
attribute	Vibrio	units	String	colony forming units/milliliter (CFU/ml)
variable	Chl_a		float
attribute	Chl_a	_FillValue	float	NaN
attribute	Chl_a	actual_range	float	0.03, 0.59
attribute	Chl_a	bcodmo_name	String	chlorophyll a
attribute	Chl_a	colorBarMaximum	double	30.0
attribute	Chl_a	colorBarMinimum	double	0.03
attribute	Chl_a	colorBarScale	String	Log
attribute	Chl_a	description	String	Chlorophyll-a concentration: determined by acetone freeze thaw using EPA method 445.0 (non-acidificaton). Data went through internal lab QAQC process. BDL= below detection limit.
attribute	Chl_a	long_name	String	Concentration Of Chlorophyll In Sea Water
attribute	Chl_a	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/
attribute	Chl_a	units	String	microgram/liter (ug/L)
variable	DOC		float
attribute	DOC	_FillValue	float	NaN
attribute	DOC	actual_range	float	61.0, 339.7
attribute	DOC	bcodmo_name	String	DOC
attribute	DOC	description	String	Dissolved organic carbon concentration: determined using oxidatve high temperature combuston-infrared analysis. MDL is 11.16 micro mol/L.
attribute	DOC	long_name	String	DOC
attribute	DOC	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CORGZZZX/
attribute	DOC	units	String	micromol/liter C (umol/L)
variable	DON		float
attribute	DON	_FillValue	float	NaN
attribute	DON	actual_range	float	1.4, 26.1
attribute	DON	bcodmo_name	String	Dissolved Organic Nitrogen
attribute	DON	description	String	Dissolved organic nitrogen concentration: determined the same as DOC samples but the sample is converted to nitrogen monoxide and measured by a chemoluminescence gas analyzer. The analyte sampled is TDN and the inorganic values are subtracted to get DON. MDL is 5.38 micro mol/L.
attribute	DON	long_name	String	DON
attribute	DON	units	String	micromol/liter N (umol/L)
variable	NH4		String
attribute	NH4	bcodmo_name	String	Ammonium
attribute	NH4	description	String	Ammonium concentration: determined by the automated phenate method 4500-NH3G. 20th Edition Std. Meth. MDL is 0.3 micro gram/L.
attribute	NH4	long_name	String	NH4
attribute	NH4	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/AMONAAZX/
attribute	NH4	units	String	micromol/liter N (umol/L)
variable	NO3		String
attribute	NO3	bcodmo_name	String	NO3
attribute	NO3	description	String	Nitrate concentration: determined by the automated cadmium reducton method 4500-NO3- F. 20th Edition Std. Method. MDL is 0.3 micro gram/L.
attribute	NO3	long_name	String	NO3
attribute	NO3	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/NTRAIGGS/
attribute	NO3	units	String	micromol/liter N (umol/L)
variable	NO2		float
attribute	NO2	_FillValue	float	NaN
attribute	NO2	actual_range	float	0.01, 0.14
attribute	NO2	bcodmo_name	String	NO2
attribute	NO2	colorBarMaximum	double	1.0
attribute	NO2	colorBarMinimum	double	0.0
attribute	NO2	description	String	Nitrite concentration: determined as with Nitrate without running the sample through a cadmium column. 20th Edition Std. Meth. MDL is 0.1 micro gram/L.
attribute	NO2	long_name	String	Mole Concentration Of Nitrite In Sea Water
attribute	NO2	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/NTRIAAZX/
attribute	NO2	units	String	micromol/liter N (umol/L)
variable	Orthophosphate		String
attribute	Orthophosphate	bcodmo_name	String	PO4
attribute	Orthophosphate	description	String	Orthophosphate concentration: determined by the automated ascorbic acid reducton method 4500-P F. 20th Edition Std. Meth. MDL is 0.2 micro gram/ L.
attribute	Orthophosphate	long_name	String	Orthophosphate
attribute	Orthophosphate	units	String	micromol/liter P (umol/L)
variable	SiO4		String
attribute	SiO4	bcodmo_name	String	SiO4
attribute	SiO4	description	String	Silicate concentration: determined by the automated molybdate-reactve silica method 4500-SiO2 E. 20th Edition Std. Meth. MDL is 0.3 micro gram/L.
attribute	SiO4	long_name	String	Si O4
attribute	SiO4	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/SLCAAAZX/
attribute	SiO4	units	String	micromol/liter Si (umol/L)
variable	dFe		float
attribute	dFe	_FillValue	float	NaN
attribute	dFe	actual_range	float	0.71, 6.38
attribute	dFe	bcodmo_name	String	Fe
attribute	dFe	description	String	dissolved iron concentration: determined in the 0.2 um filtered fracton using ICP-MSas described in Milne et al. 2010. Analytca Chimera Acta 665: 200-207
attribute	dFe	long_name	String	D Fe
attribute	dFe	units	String	nanoMolar (nM)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.