BCO-DMO ERDDAP
Accessing BCO-DMO data
log in    
Brought to you by BCO-DMO    

ERDDAP > info > bcodmo_dataset_699469

Grid
DAP
Data
Sub-
set
Table
DAP
Data
Make
A
Graph
W
M
S
Source
Data
Files
Acces-
sible
?
Title Sum-
mary
FGDC,
ISO,
Metadata
Back-
ground
Info
RSS E
mail
Institution Dataset ID
   set  data   graph     files  public [Clear Lake 800-yr reconstruction] - Record of d2H of dinosterol variability in down core
lake sediments from Clear Lake, Palau (Do Parallel Patterns Arise from Parallel Processes?)
   ?   F   I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_699469

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Sampling and analytical procedures:
Clear Lake sediments were extracted using a Dionex Accelerated Solvent
Extractor (ASE 200), and the resulting total lipid extract was separated into
neutral and polar fractions using column chromatography with aminopropyl gel
as the stationary phase. The neutral fraction was then separated into
hydrocarbon, wax ester, sterol and polar fractions via a silica gel column
chromatography. Dinosterol was isolated then from the sterol fraction via
reverse phase (RP)- high-performance liquid chromatography (HPLC)\u2028.

Instruments:
Dinosterol was isolated from the sterol fraction via reverse phase (RP)-
high performance liquid chromatography (HPLC). An Agilent 1100 HPLC with an
integrated autoinjector, quaternary pump, and fraction collector was coupled
to an Agilent 1100 LC/MSD SL mass spectrometer with a multimode source that
was operated in positive atmospheric pressure chemical ionization (APCI+)
mode. The HPLC method used is outlined in Nelson and Sachs
(2013).\u00a0Subsequently to HPLC separation the fraction containing
dinosterol was analyzed via GC-MSD to verify sufficient baseline separation.
Adjacent HPLC fractions were also analyzed to ensure that no dinosterol eluted
into those fractions.

After the dinosterol was sufficiently isolated from co-eluting compounds, the
sample was injected onto a GC-irms for determination of the d2H of
dinosterol.\u00a0 Hydrogen isotope determinations were made using a Finnigan
Delta V Plus Isotope Ratio Mass Spectrometer (irMS) coupled to a Thermo Trace
GC Ultra with a Varian VF-17ms FactorFour capillary column (60 m x 0.32 mm x
0.25 m) and a pyrolysis reactor. Samples were injected into a split/splitless
inlet in splitless mode at 310 C. The oven temperature was ramped from 100 C
to 220 C at a rate of 20 C/min, then at 2 C /min up to 325 C where it was held
for 17 min. The carrier gas, He, was held constant at 2.6 mL/min. The
pyroloysis reactor was maintained at 1400 C. Isotope values, expressed as D
values, were calculated in Isodat software relative to VSMOW using a co-
injection standard containing nC28 nC32, nC40, and nC44 of known \u22022H
values (obtained from Arndt Schimmelmann, Indiana University, Bloomington, IN,
USA). The measured isotope values of dinosterol were corrected for the
addition of hydrogen atoms (of known D value) that occurred during
acetylation. Each sample was analyzed in at least duplicate, and error bars
represent standard deviations of replicate measurements.
attribute NC_GLOBAL awards_0_award_nid String 55104
attribute NC_GLOBAL awards_0_award_number String OCE-1241247
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1241247 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Clear Lake 800-yr reconstruction
Record of d2H of dinosterol variability in down core lake sediments
PI: Julian P. Sachs (University of Washington)
Co-PI: Julie N. Richey (University of Washington)
Version: 04 May 2017
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2017-05-04T19:36:35Z
attribute NC_GLOBAL date_modified String 2019-08-02T19:07:33Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.699469.1
attribute NC_GLOBAL Easternmost_Easting double 134.3594
attribute NC_GLOBAL geospatial_lat_max double 7.153166667
attribute NC_GLOBAL geospatial_lat_min double 7.153166667
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double 134.3594
attribute NC_GLOBAL geospatial_lon_min double 134.3594
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/699469 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String IR Mass Spec
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Hydrogen isotope determinations were made using a Finnigan Delta V Plus Isotope Ratio Mass Spectrometer (irMS) coupled to a Thermo Trace GC Ultra with a Varian VF-17ms FactorFour capillary column (60 m x 0.32 mm x 0.25 m) and a pyrolysis reactor.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 699743
attribute NC_GLOBAL instruments_0_description String The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB16/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Isotope-ratio Mass Spectrometer
attribute NC_GLOBAL instruments_0_instrument_nid String 469
attribute NC_GLOBAL instruments_0_supplied_name String Finnigan Delta V Plus Isotope Ratio Mass Spectrometer (irMS)
attribute NC_GLOBAL instruments_1_acronym String HPLC
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Dinosterol was isolated from the sterol fraction via reverse phase (RP)- high performance liquid chromatography (HPLC). An Agilent 1100 HPLC with an integrated autoinjector, quaternary pump, and fraction collector was coupled to an Agilent 1100 LC/MSD SL mass spectrometer with a multimode source that was operated in positive atmospheric pressure chemical ionization (APCI+) mode.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 699741
attribute NC_GLOBAL instruments_1_description String A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB11/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String High Performance Liquid Chromatograph
attribute NC_GLOBAL instruments_1_instrument_nid String 506
attribute NC_GLOBAL instruments_1_supplied_name String high-performance liquid chromatography
attribute NC_GLOBAL instruments_2_acronym String Mass Spec
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Dinosterol was isolated from the sterol fraction via reverse phase (RP)- high performance liquid chromatography (HPLC). An Agilent 1100 HPLC with an integrated autoinjector, quaternary pump, and fraction collector was coupled to an Agilent 1100 LC/MSD SL mass spectrometer with a multimode source that was operated in positive atmospheric pressure chemical ionization (APCI+) mode.
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 699742
attribute NC_GLOBAL instruments_2_description String General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB16/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Mass Spectrometer
attribute NC_GLOBAL instruments_2_instrument_nid String 685
attribute NC_GLOBAL instruments_2_supplied_name String Agilent 1100 LC/MSD SL mass spectrometer
attribute NC_GLOBAL keywords String age, bco, bco-dmo, biological, chemical, core, core_depth, data, dataset, dDdino, dDdino_stdev, ddino, depth, deviation, dmo, erddap, latitude, longitude, management, oceanography, office, preliminary, standard, standard deviation, stdev
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/699469/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/699469 (external link)
attribute NC_GLOBAL Northernmost_Northing double 7.153166667
attribute NC_GLOBAL param_mapping String {'699469': {'lat': 'master - latitude', 'lon': 'master - longitude', 'core_depth': 'flag - depth'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/699469/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Washington
attribute NC_GLOBAL people_0_affiliation_acronym String UW
attribute NC_GLOBAL people_0_person_name String Julian P. Sachs
attribute NC_GLOBAL people_0_person_nid String 51578
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of Washington
attribute NC_GLOBAL people_1_affiliation_acronym String UW
attribute NC_GLOBAL people_1_person_name String Julie N. Richey
attribute NC_GLOBAL people_1_person_nid String 699725
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Shannon Rauch
attribute NC_GLOBAL people_2_person_nid String 51498
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String PaPaPro
attribute NC_GLOBAL projects_0_acronym String PaPaPro
attribute NC_GLOBAL projects_0_description String This project will survey the taxonomic, genetic, and functional diversity of the organisms found in marine lakes, and investigate the processes that cause gains and losses in this biodiversity. Marine lakes formed as melting ice sheets raised sea level after the last glacial maximum and flooded hundreds of inland valleys around the world. Inoculated with marine life from the surrounding sea and then isolated to varying degrees for the next 6,000 to 15,000 years, these marine lakes provide multiple, independent examples of how environments and interactions between species can drive extinction and speciation. Researchers will survey the microbes, algae, invertebrates, and fishes present in 40 marine lakes in Palau and Papua, and study how diversity has changed over time by retrieving the remains of organisms preserved in sediments on the lake bottoms. The project will test whether the number of species, the diversity of functional roles played by organisms, and the genetic diversity within species increase and decrease in parallel; whether certain species can greatly curtail diversity by changing the environment; whether the size of a lake determines its biodiversity; and whether the processes that control diversity in marine organisms are similar to those that operate on land.
Because biodiversity underlies the ecosystem services on which society depends, society has a great interest in understanding the processes that generate and retain biodiversity in nature. This project will also help conserve areas of economic importance. Marine lakes in the study region are important for tourism, and researchers will work closely with governmental and non-governmental conservation and education groups and with diving and tourism businesses to raise awareness of the value and threats to marine lakes in Indonesia and Palau.
attribute NC_GLOBAL projects_0_end_date String 2017-12
attribute NC_GLOBAL projects_0_geolocation String Western Pacific; Palau; Indonesia (West Papua)
attribute NC_GLOBAL projects_0_name String Do Parallel Patterns Arise from Parallel Processes?
attribute NC_GLOBAL projects_0_project_nid String 2238
attribute NC_GLOBAL projects_0_project_website String http://marinelakes.ucmerced.edu/ (external link)
attribute NC_GLOBAL projects_0_start_date String 2013-01
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 7.153166667
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String location,latitude,longitude
attribute NC_GLOBAL summary String Record of d2H of dinosterol variability in down core lake sediments from Clear Lake, Palau.
attribute NC_GLOBAL title String [Clear Lake 800-yr reconstruction] - Record of d2H of dinosterol variability in down core lake sediments from Clear Lake, Palau (Do Parallel Patterns Arise from Parallel Processes?)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double 134.3594
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable location   String  
attribute location bcodmo_name String site
attribute location description String Name of the sampling location
attribute location long_name String Location
attribute location units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 7.153166667, 7.153166667
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude of the location
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double 134.3594, 134.3594
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude of the location
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable core_depth   double  
attribute core_depth _FillValue double NaN
attribute core_depth actual_range double 0.5, 87.5
attribute core_depth bcodmo_name String depth_bsf
attribute core_depth description String Depth below sediment-water interface
attribute core_depth long_name String Core Depth
attribute core_depth units String centimeters (cm)
variable age   short  
attribute age _FillValue short 32767
attribute age actual_range short 1284, 2002
attribute age bcodmo_name String age
attribute age description String Calendar age of sample in year A.D.
attribute age long_name String Age
attribute age units String unitless
variable dDdino   float  
attribute dDdino _FillValue float NaN
attribute dDdino actual_range float -317.9, -266.9
attribute dDdino bcodmo_name String unknown
attribute dDdino description String d2Hdino: hydrogen isotopic composition of dinosterol
attribute dDdino long_name String D Ddino
attribute dDdino units String per mil
variable dDdino_stdev   float  
attribute dDdino_stdev _FillValue float NaN
attribute dDdino_stdev actual_range float 0.2, 10.7
attribute dDdino_stdev bcodmo_name String unknown
attribute dDdino_stdev description String 1 sigma standard deviation on d2Hdino
attribute dDdino_stdev long_name String D Ddino Stdev
attribute dDdino_stdev units String per mil

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
ERDDAP, Version 2.22
Disclaimers | Privacy Policy | Contact