BCO-DMO ERDDAP
Accessing BCO-DMO data
log in    
Brought to you by BCO-DMO    

ERDDAP > info > bcodmo_dataset_700961

Grid
DAP
Data
Sub-
set
Table
DAP
Data
Make
A
Graph
W
M
S
Source
Data
Files
Acces-
sible
?
Title Sum-
mary
ISO,
Metadata
Back-
ground
Info
RSS E
mail
Institution Dataset ID
   set  data   graph     files  public NCBI Sequence Read Archive (SRA) accession numbers for fastq sequence files for each
zooplankton community sample (Plankton Population Genetics project)
   ?     I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_700961

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String SAMPLE INFORMATION

Sample identifiers include the following codes.

MOCNESS tow
FA3: Night sampling
FA4: Day sampling

Depth range:
N1: 1500-1000m
N2: 1000-700m
N3: 700-500m
N4: 500-300m
N5: 300-200m
N6: 200-150m
N7: 150-100m
N8: 100-50m
N9: 50m-0m

Wet-sieved zooplankton size fractions
SF1: 0.2-0.5 mm
SF2: 0.5-1.0 mm
SF3: 1.0-2.0 mm
attribute NC_GLOBAL awards_0_award_nid String 473046
attribute NC_GLOBAL awards_0_award_number String OCE-1255697
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=1255697 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL awards_1_award_nid String 537990
attribute NC_GLOBAL awards_1_award_number String OCE-1338959
attribute NC_GLOBAL awards_1_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1338959 (external link)
attribute NC_GLOBAL awards_1_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_1_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_1_funding_source_nid String 355
attribute NC_GLOBAL awards_1_program_manager String David L. Garrison
attribute NC_GLOBAL awards_1_program_manager_nid String 50534
attribute NC_GLOBAL awards_2_award_nid String 539716
attribute NC_GLOBAL awards_2_award_number String OCE-1029478
attribute NC_GLOBAL awards_2_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1029478 (external link)
attribute NC_GLOBAL awards_2_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_2_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_2_funding_source_nid String 355
attribute NC_GLOBAL awards_2_program_manager String David L. Garrison
attribute NC_GLOBAL awards_2_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String ALOHA Zooplankton metabarcoding: SRA
PI: Erica Goetze
data version: 07 Jun 2017
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2017-05-25T19:52:10Z
attribute NC_GLOBAL date_modified String 2017-08-02T12:08:50Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.704665
attribute NC_GLOBAL Easternmost_Easting double -158.0
attribute NC_GLOBAL geospatial_lat_max double 22.75
attribute NC_GLOBAL geospatial_lat_min double 22.75
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -158.0
attribute NC_GLOBAL geospatial_lon_min double -158.0
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/700961 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Automated Sequencer
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Illumina MiSeq using V3 chemistry (300-bp, paired-end)
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 700970
attribute NC_GLOBAL instruments_0_description String General term for a laboratory instrument used for deciphering the order of bases in a strand of DNA. Sanger sequencers detect fluorescence from different dyes that are used to identify the A, C, G, and T extension reactions. Contemporary or Pyrosequencer methods are based on detecting the activity of DNA polymerase (a DNA synthesizing enzyme) with another chemoluminescent enzyme. Essentially, the method allows sequencing of a single strand of DNA by synthesizing the complementary strand along it, one base pair at a time, and detecting which base was actually added at each step.
attribute NC_GLOBAL instruments_0_instrument_name String Automated DNA Sequencer
attribute NC_GLOBAL instruments_0_instrument_nid String 649
attribute NC_GLOBAL instruments_0_supplied_name String Illumina MiSeq
attribute NC_GLOBAL instruments_1_acronym String Thermal Cycler
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 700972
attribute NC_GLOBAL instruments_1_description String General term for a laboratory apparatus commonly used for performing polymerase chain reaction (PCR). The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps.

(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)
attribute NC_GLOBAL instruments_1_instrument_name String PCR Thermal Cycler
attribute NC_GLOBAL instruments_1_instrument_nid String 471582
attribute NC_GLOBAL instruments_1_supplied_name String quantitative PCR by the Evolutionary Genetics Core Facility (Hawaii Institute of Marine Biology)
attribute NC_GLOBAL instruments_2_acronym String Bioanalyzer
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 700971
attribute NC_GLOBAL instruments_2_description String A Bioanalyzer is a laboratory instrument that provides the sizing and quantification of DNA, RNA, and proteins. One example is the Agilent Bioanalyzer 2100.
attribute NC_GLOBAL instruments_2_instrument_name String Bioanalyzer
attribute NC_GLOBAL instruments_2_instrument_nid String 626182
attribute NC_GLOBAL instruments_2_supplied_name String Agilent 2100 Bioanalyzer
attribute NC_GLOBAL keywords String accession, analysis, analysis_name, bco, bco-dmo, biological, bioproject, bioproject_accession, bioproject_link, biosample, biosample_accession, biosample_link, chemical, data, dataset, description, design, design_description, dmo, erddap, instrument, instrument_model, latitude, layout, library, library_ID, library_layout, library_selection, library_source, library_strategy, link, longitude, management, model, name, oceanography, office, platform, preliminary, selection, source, strategy, title
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/700961/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/700961 (external link)
attribute NC_GLOBAL Northernmost_Northing double 22.75
attribute NC_GLOBAL param_mapping String {'700961': {'lat': 'master - latitude', 'lon': 'master - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/700961/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Hawaii
attribute NC_GLOBAL people_0_person_name String Erica Goetze
attribute NC_GLOBAL people_0_person_nid String 473048
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of Hawaii
attribute NC_GLOBAL people_1_person_name String Erica Goetze
attribute NC_GLOBAL people_1_person_nid String 473048
attribute NC_GLOBAL people_1_role String Contact
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Amber York
attribute NC_GLOBAL people_2_person_nid String 643627
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String Plankton Population Genetics
attribute NC_GLOBAL projects_0_acronym String Plankton Population Genetics
attribute NC_GLOBAL projects_0_description String Description from NSF award abstract:
Marine zooplankton show strong ecological responses to climate change, but little is known about their capacity for evolutionary response. Many authors have assumed that the evolutionary potential of zooplankton is limited. However, recent studies provide circumstantial evidence for the idea that selection is a dominant evolutionary force acting on these species, and that genetic isolation can be achieved at regional spatial scales in pelagic habitats. This RAPID project will take advantage of a unique opportunity for basin-scale transect sampling through participation in the Atlantic Meridional Transect (AMT) cruise in 2014. The cruise will traverse more than 90 degrees of latitude in the Atlantic Ocean and include boreal-temperate, subtropical and tropical waters. Zooplankton samples will be collected along the transect, and mitochondrial and microsatellite markers will be used to identify the geographic location of strong genetic breaks within three copepod species. Bayesian and coalescent analytical techniques will test if these regions act as dispersal barriers. The physiological condition of animals collected in distinct ocean habitats will be assessed by measurements of egg production (at sea) as well as body size (condition index), dry weight, and carbon and nitrogen content. The PI will test the prediction that ocean regions that serve as dispersal barriers for marine holoplankton are areas of poor-quality habitat for the target species, and that this is a dominant mechanism driving population genetic structure in oceanic zooplankton.
Note: This project is funded by an NSF RAPID award. This RAPID grant supported the shiptime costs, and all the sampling reported in the AMT24 zooplankton ecology cruise report (PDF).
Online science outreach blog at: https://atlanticplankton.wordpress.com
attribute NC_GLOBAL projects_0_end_date String 2015-11
attribute NC_GLOBAL projects_0_geolocation String Atlantic Ocean, 46 N - 46 S
attribute NC_GLOBAL projects_0_name String Basin-scale genetics of marine zooplankton
attribute NC_GLOBAL projects_0_project_nid String 537991
attribute NC_GLOBAL projects_0_start_date String 2013-12
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 22.75
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String latitude,longitude,bioproject_accession,title,library_strategy,library_source,library_selection,library_layout,platform,instrument_model,design_description,bioproject_link
attribute NC_GLOBAL summary String These data include sample information and accession links to sequence data at
The National Center for Biotechnology Information (NCBI) Sequence Read Archive
(SRA).

This data submission consists of metabarcoding data for the zooplankton
community in the epipelagic, mesopelagic and upper bathypelagic zones
(0-1500m) of the North Pacific Subtropical Gyre. The goal of this study was to
assess the hidden diversity present in zooplankton assemblages in midwaters,
and detect vertical gradients in species richness, depth distributions, and
community composition of the full zooplankton assemblage. Samples were
collected in June 2014 from Station ALOHA (22.75, -158) using a 1 meter square
Multiple Opening and Closing Nets and Environmental Sampling System (MOCNESS,
200um mesh), on R/V Falkor cruise FK140613. \u00a0Next generation sequence
data (Illumina MiSeq, V3 chemistry, 300-bp paired-end) of the zooplankton
assemblage derive from amplicons of the V1-V2 region of 18S rRNA (primers
described in Fonseca et al. 2010). The data includes sequences and read count
abundance information for molecular OTUs from both holoplanktonic and
meroplanktonic taxa

Related dataset containing OTU tables and fasta sequences (representative /
most abundance read for each OTU):
[Metabarcoding zooplankton at ](\\http://www.bco-
dmo.org/dataset/700279\\)[station](\\http://www.bco-
dmo.org/dataset/700279\\)[ ALOHA: OTU tables and ](\\http://www.bco-
dmo.org/dataset/700279\\)[fasta](\\http://www.bco-
dmo.org/dataset/700279\\)[ files](\\http://www.bco-
dmo.org/dataset/700279\\)
attribute NC_GLOBAL title String NCBI Sequence Read Archive (SRA) accession numbers for fastq sequence files for each zooplankton community sample (Plankton Population Genetics project)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -158.0
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable analysis_name   String  
attribute analysis_name bcodmo_name String sample_descrip
attribute analysis_name description String Descriptive name of analysis including percent clustering and subsampling or no subsampling
attribute analysis_name long_name String Analysis Name
attribute analysis_name units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 22.75, 22.75
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude of sample site (Station ALOHA)
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -158.0, -158.0
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude of sample site (Station ALOHA)
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable library_ID   String  
attribute library_ID bcodmo_name String sample
attribute library_ID description String Short unique identifier for the sequencing library.
attribute library_ID long_name String Library ID
attribute library_ID nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute library_ID units String unitless
variable bioproject_accession   String  
attribute bioproject_accession bcodmo_name String accession number
attribute bioproject_accession description String NCBI BioProject identifier
attribute bioproject_accession long_name String Bioproject Accession
attribute bioproject_accession units String unitless
variable biosample_accession   String  
attribute biosample_accession bcodmo_name String accession number
attribute biosample_accession description String NCBI BioSample identifier
attribute biosample_accession long_name String Biosample Accession
attribute biosample_accession units String unitless
variable title   String  
attribute title bcodmo_name String sample_descrip
attribute title description String Short description that will identify the dataset on public pages.
attribute title long_name String Title
attribute title units String unitless
variable library_strategy   String  
attribute library_strategy bcodmo_name String sample_descrip
attribute library_strategy description String Amplicon = Sequencing of overlapping or distinct PCR or RT-PCR products
attribute library_strategy long_name String Library Strategy
attribute library_strategy units String unitless
variable library_source   String  
attribute library_source bcodmo_name String sample_descrip
attribute library_source description String Metagenomic = Mixed material from metagenome
attribute library_source long_name String Library Source
attribute library_source units String unitless
variable library_selection   String  
attribute library_selection bcodmo_name String sample_descrip
attribute library_selection description String PCR = Source material was selected by designed primers
attribute library_selection long_name String Library Selection
attribute library_selection units String unitless
variable library_layout   String  
attribute library_layout bcodmo_name String sample_descrip
attribute library_layout description String Paired-end or Single
attribute library_layout long_name String Library Layout
attribute library_layout units String unitless
variable platform   String  
attribute platform bcodmo_name String sample_descrip
attribute platform description String Sequencing platforms [Illumina]
attribute platform long_name String Platform
attribute platform units String unitless
variable instrument_model   String  
attribute instrument_model bcodmo_name String instrument
attribute instrument_model description String Illumina instrument and model used for sequencing
attribute instrument_model long_name String Instrument Model
attribute instrument_model units String unitless
variable design_description   String  
attribute design_description bcodmo_name String sample_descrip
attribute design_description description String Free-form description of the methods used to create the sequencing library; a brief materials and methods section.
attribute design_description long_name String Design Description
attribute design_description units String unitless
variable bioproject_link   String  
attribute bioproject_link bcodmo_name String unknown
attribute bioproject_link description String bioproject_link
attribute bioproject_link long_name String Bioproject Link
attribute bioproject_link units String unitless
variable biosample_link   String  
attribute biosample_link bcodmo_name String unknown
attribute biosample_link description String biosample_link
attribute biosample_link long_name String Biosample Link
attribute biosample_link units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
ERDDAP, Version 2.02
Disclaimers | Privacy Policy | Contact