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Title Sum-
RSS Institution Dataset ID
     data   graph     files  public Fe(II) concentrations over time in iron oxidizing bacteria cultures.    ?     I   M   background (external link) RSS BCO-DMO bcodmo_dataset_709440

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String These data were collected by placing each strain in a 100 mL serum vial with 6
mL of their standard, published media with 30 mg zero valent iron as a source
of Fe(II).\u00a0 The headspace was filled with a gas mix of 8% oxygen/10%
carbon dioxide/82% nitrogen by using bottled gas mixes and a regulator to
flush the headspace without overpressurization. Each vial was sampled once per
day and the Fe(II) concentration was determined by ferrozine assay.
attribute NC_GLOBAL awards_0_award_nid String 626092
attribute NC_GLOBAL awards_0_award_number String OCE-1459252
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=1459252 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Dr David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Timecourses
P. Girguis and D. Emerson, PIs
Version 21 July 2017
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.2d 13 Jun 2019
attribute NC_GLOBAL date_created String 2017-07-21T18:03:59Z
attribute NC_GLOBAL date_modified String 2019-03-26T18:32:09Z
attribute NC_GLOBAL defaultDataQuery String &time
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.709440.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/709440 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Spectrophotometer
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to measure ferrozine assay
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 709453
attribute NC_GLOBAL instruments_0_description String An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB20/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Spectrophotometer
attribute NC_GLOBAL instruments_0_instrument_nid String 707
attribute NC_GLOBAL instruments_0_supplied_name String Cary 100 UV-Vis Spectrophotometer (Agilent Technologies)
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, chemical, concentration, data, dataset, date, deviation, dmo, erddap, Fe_II_concentration, management, oceanography, office, preliminary, standard, standard_deviation, statistics, time, time2, vial
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/709440 (external link)
attribute NC_GLOBAL param_mapping String {'709440': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/709440/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Harvard University
attribute NC_GLOBAL people_0_person_name String Dr Peter Girguis
attribute NC_GLOBAL people_0_person_nid String 544586
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Bigelow Laboratory for Ocean Sciences
attribute NC_GLOBAL people_1_person_name String Dr David Emerson
attribute NC_GLOBAL people_1_person_nid String 544585
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Harvard University
attribute NC_GLOBAL people_2_person_name String Jacob Cohen
attribute NC_GLOBAL people_2_person_nid String 650331
attribute NC_GLOBAL people_2_role String Contact
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Hannah Ake
attribute NC_GLOBAL people_3_person_nid String 650173
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String Collaborative Research: The Role of Iron-oxidizing Bacteria in the Sedimentary Iron Cycle: Ecological, Physiological and Biogeochemical Implications
attribute NC_GLOBAL projects_0_acronym String SedimentaryIronCycle
attribute NC_GLOBAL projects_0_description String Iron is a critical element for life that serves as an essential trace element for eukaryotic organisms. It is also able to support the growth of a cohort of microbes that can either gain energy for growth via oxidation of ferrous (Fe(II)) to ferric (Fe(III)) iron, or by utilizing Fe(III) for anaerobic respiration coupled to oxidation of simple organic matter or H2. This coupled process is referred to as the microbial iron cycle. One of the primary sources of iron to the ocean comes from dissolved iron (dFe) that is produced through oxidation and reduction processes in the sediment where iron is abundant. The dFe is transported into the overlaying water where it is an essential nutrient for phytoplankton responsible for primary production in the world’s oceans. In fact, iron limitation significantly impacts production in as much as a third of the world’s open oceans. The basic geochemistry of this process is understood; however important gaps exist in our knowledge about the details of how the iron cycle works, and how critical a role bacteria play in it.
Intellectual Merit. Conventional wisdom holds that most of the iron oxidation in sediments is abiological, as a result of the rapid kinetics of chemical iron oxidation in the presence of oxygen. This proposal aims to question this conventional view and enhance our understanding of the microbes involved in the sedimentary iron cycle, with an emphasis on the bacteria that catalyze the oxidation of iron. These Fe-oxidizing bacteria (FeOB) utilize iron as a sole energy source for growth, and are autotrophic.� They were only discovered in the ocean about forty-five years ago, and are now known to be abundant at hydrothermal vents that emanate ferrous-rich fluids. More recently, the first evidence was published that they could inhabit coastal sediments, albeit at reduced numbers, and even be abundant in some continental shelf sediments. These habitats are far removed from hydrothermal vents, and reveal the sediments may be an important habitat for FeOB that live on ferrous iron generated in the sediment. This begs the question: are FeOB playing an important role in the oxidative part of the sedimentary Fe-cycle? One important attribute of FeOB is their ability to grow at very low levels of O2, an essential strategy for them to outcompete chemical iron oxidation. How low a level of O2 can sustain them, and how this might affect their distribution in sediments is unknown. In part, this is due to the technical challenges of measuring O2 concentrations and dynamics at very low levels; yet these concentrations could be where FeOB flourish. The central hypothesis of this proposal is that FeOB are more common in marine sedimentary environments than previously recognized, and play a substantive role in governing the iron flux from the sediments into the water column by constraining the release of dFe from sediments. A set of experimental objectives are proposed to test this. A survey of near shore regions in the Gulf of Maine, and a transect along the Monterey Canyon off the coast of California will obtain cores of sedimentary muds and look at the vertical distribution of FeOB and putative Fe-reducing bacteria using sensitive techniques to detect their presence and relative abundance. Some of these same sediments will be used in a novel reactor system that will allow for precise control of O2 levels and iron concentration to measure the dynamics of the iron cycle under different oxygen regimens. Finally pure cultures of FeOB with different O2 affinities will be tested in a bioreactor coupled to a highly sensitive mass spectrometer to determine the lower limits of O2 utilization for different FeOB growing on iron, thus providing mechanistic insight into their activity and distribution in low oxygen environments.
Broader Impacts. An important impact of climate change on marine environments is a predicted increase in low O2 or hypoxic zones in the ocean. Hypoxia in association with marine sediments will have a profound influence on the sedimentary iron cycle, and is likely to lead to greater inputs of dFe into the ocean. In the longer term, this increase in dFe flux could alleviate iron-limitation in some regions of the ocean, thereby enhancing the rate of CO2-fixation and draw down of CO2 from the atmosphere. This is one important reason for developing a better understanding of microbial control of sedimentary iron cycle. This project will also provide training to a postdoctoral scientist, graduate students and undergraduates. This project will contribute to a student initiated exhibit, entitled ‘Iron and the evolution of life on Earth’ at the Harvard Museum of Natural History providing a unique opportunity for undergraduate training and outreach.
attribute NC_GLOBAL projects_0_geolocation String Intertidal coastal river and coastal shelf sediments, mid-coast, Maine, USA; Monteray Bay Canyon, sediments, CA, USA
attribute NC_GLOBAL projects_0_name String Collaborative Research: The Role of Iron-oxidizing Bacteria in the Sedimentary Iron Cycle: Ecological, Physiological and Biogeochemical Implications
attribute NC_GLOBAL projects_0_project_nid String 544584
attribute NC_GLOBAL publisher_name String Hannah Ake
attribute NC_GLOBAL publisher_role String BCO-DMO Data Manager(s)
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v29
attribute NC_GLOBAL summary String Fe(II) concentrations over time in iron oxidizing bacteria cultures.
attribute NC_GLOBAL title String Fe(II) concentrations over time in iron oxidizing bacteria cultures.
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5-beta
variable vial   String  
attribute vial description String [strain] ASWkan replacement description
attribute vial ioos_category String Unknown
attribute vial long_name String Vial
attribute vial units String unitless
variable date   String  
attribute date description String Date measurement was taken; YYYY/MM/DD
attribute date ioos_category String Time
attribute date long_name String Date
attribute date source_name String date
attribute date units String unitless
variable time2   float  
attribute time2 _FillValue float NaN
attribute time2 actual_range float 20.0, 114.5
attribute time2 description String Time since start of experiment
attribute time2 ioos_category String Time
attribute time2 long_name String Time
attribute time2 units String hours
variable Fe_II_concentration   double  
attribute Fe_II_concentration _FillValue double NaN
attribute Fe_II_concentration actual_range double 0.17383495, 1.57250016
attribute Fe_II_concentration description String Concentration of Fe(II) in media (average of triplicate samples)
attribute Fe_II_concentration ioos_category String Unknown
attribute Fe_II_concentration long_name String Fe II Concentration
attribute Fe_II_concentration units String mM
variable standard_deviation   double  
attribute standard_deviation _FillValue double NaN
attribute standard_deviation actual_range double 7.44154E-4, 0.129878975
attribute standard_deviation colorBarMaximum double 50.0
attribute standard_deviation colorBarMinimum double 0.0
attribute standard_deviation description String Standard deviation of triplicate samples
attribute standard_deviation ioos_category String Statistics
attribute standard_deviation long_name String Standard Deviation
attribute standard_deviation units String mM

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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