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   set  data   graph     files  public [Initial spores] - Pterosiphonia bipinnata and Corallina vancouveriensis initial experiment
attachment and duration at Deadman Bay on San Juan Island, Washington (Effects of Ocean
Acidification on Coastal Organisms: An Ecomaterials Perspective)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Sporophytic specimens of Pterosiphonia bipinnata and Corallina vancouveriensis
were collected from the mid to low intertidal zone on San Juan Island,
Washington (Deadman Bay: 48 30' 48.93\"N, 123 8'56.14\"W), immediately
transported to Friday Harbor Laboratories (FHL), and maintained in an outdoor
seawater table for up to one week before testing. Reproductive parent fronds
were held at ambient levels of seawater pH/pCO2 and were only exposed to
experimental conditions while releasing spores. Spore release and settlement
was performed in the Ocean Acidification Environmental Laboratory (OAEL) at
FHL, allowing precise control of pH and temperature in a flow-through system.
Two pH treatments were established by bubbling CO2 (7.75, 7.30 total scale) at
11C and confirmed with carbonate water chemistry analyses. Specifically,
spectrophotometric pH was determined as per SOP6 and total alkalinity was
measured using an open cell titrator as outlined in SOP3b (Dickson et al.
2007). Ambient pH values for the Salish Sea are approximately 7.8, and pH as
low as 7.3 has been documented in nearshore environments in Washington.\u00a0

\u00a0The spore settlement apparatus consisted of a carriage system that
slowly (1-2.5 cm/hr.) drove reproductive algal thalli across a glass
settlement plate (0.6 x 7.6 x 60 cm), while releasing spores. The working
section (14 x 1.5 cm) was defined at one end of the plate. Spores landing in
the working section had a decreasing gradient of attachment time, determined
by the rate parent thalli were driven across the plate and the time the plate
was allowed to set. Error in attachment time was estimated to be 6-12 minutes
given sinking rates of spores of comparable spore size (50-100 um) and release
height (5-10 cm).

The shear flume was designed to release a tall column of water that flushes
quickly across the working section of the spore settlement plate. Water column
height was varied to create a range of shear stresses. \u00a0Shear stresses
generated by each water column height were calculated according to Schultz et
al. 2000, using the height of the channel (4.3 mm), the pressure gradient
across the working section (measured with a manometer), and the length of the
working section. \u00a0Most spores were removed in the first 10 seconds of
continuous exposure to shear and longer exposure times result in little
additional detachment, so trials were limited to 15 seconds.\u00a0

Prior to each test, the shear flume was fitted on the settlement plate with
clamps and the released spores were photographed using a microscope
(Steindorff SXC, New York Microscopes) connected to a camera (Nikon Coolpix
S3300), using the lines drawn in the working section for reference. For the
attachment time assay, a low shear stress (1 Pa) was applied and the remaining
spores were photographed and counted.\u00a0

For the attachment strength assay, spores were allowed to set in stationary
water for 35-48 hours in P. bipinnata and for 5-10 hours in C.
vancouveriensis, since maximum attachment of each species was found in these
time frames using the logistic regression analyses of attachment time. For P.
bipinnata, replication was achieved by repeating the experiment in time since
one frond moved across the entire settlement plate in each run. Due to
limitations with C. vancouveriensis spore release, several fronds were used to
provide sufficient spores for this assay. In this case, each frond that
released spores was considered a replicate, and this was repeated across
several days. All spore releases were natural, as attempts to artificially
induce spore release (osmotic, temperature, or light) were unsuccessful.\u00a0

Attached spores were exposed to increasing shear stresses (1, 4, 7, 17 and 20
Pa). Shear stresses in the flume represented boundary layer velocities of 0.2
\u2013 4 ms-1, similar to intertidal field conditions. At each position and at
each shear stress applied, spores were counted through photo-analysis in
ImageJ (version 1.48; U.S. National Institutes of Health, Bethesda, MD). With
each successive application of shear stress, we expected an increasing
percentage of spores to detach from the settlement plate, up to a maximum
value assuming some spores would be stronger than our assay. The effect of
shear stress on spore detachment in each species was tested using an
exponential rise-to-maximum non-linear regression (SigmaPlot 11.0, R2 = 0.84 -
0.99), which provided estimates of two parameters: the maximum percentage of
spores detached and the initial dependence of detachment on shear stress. The
first derivatives of fitted, non-linear regressions were plotted to estimate
frequency distributions of spore attachment strength.
attribute NC_GLOBAL awards_0_award_nid String 55120
attribute NC_GLOBAL awards_0_award_number String OCE-1041213
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=1041213 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Mary Beth Saffo
attribute NC_GLOBAL awards_0_program_manager_nid String 51608
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Pterosiphonia bipinnata and Corallina vancouveriensis initial experiment attachment and duration at Deadman Bay on San Juan Island, Washington
PI: Emily Carrington
Version: 2019-10-01
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2017-07-27T17:12:24Z
attribute NC_GLOBAL date_modified String 2019-10-29T15:22:25Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.710033.1
attribute NC_GLOBAL Easternmost_Easting double -123.14893
attribute NC_GLOBAL geospatial_lat_max double 48.51359
attribute NC_GLOBAL geospatial_lat_min double 48.51359
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -123.14893
attribute NC_GLOBAL geospatial_lon_min double -123.14893
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/710033 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String camera
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Prior to each test, the shear flume was fitted on the settlement plate with clamps and the released spores were photographed using a microscope (Steindorff SXC, New York Microscopes) connected to a camera (Nikon Coolpix S3300), using the lines drawn in the working section for reference.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 710040
attribute NC_GLOBAL instruments_0_description String All types of photographic equipment including stills, video, film and digital systems.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/311/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Camera
attribute NC_GLOBAL instruments_0_instrument_nid String 520
attribute NC_GLOBAL instruments_0_supplied_name String camera (Nikon Coolpix S3300)
attribute NC_GLOBAL instruments_1_acronym String Pressure Sensor
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Pressure gradient across the working section (measured with a manometer).
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 710038
attribute NC_GLOBAL instruments_1_description String A pressure sensor is a device used to measure absolute, differential, or gauge pressures. It is used only when detailed instrument documentation is not available.
attribute NC_GLOBAL instruments_1_instrument_name String Pressure Sensor
attribute NC_GLOBAL instruments_1_instrument_nid String 567
attribute NC_GLOBAL instruments_1_supplied_name String manometer
attribute NC_GLOBAL instruments_2_acronym String Water Temp Sensor
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Specifically, spectrophotometric pH was determined as per SOP6 and total alkalinity was measured using an open cell titrator as outlined in SOP3b. As described in Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to Best Practices for Ocean CO2 Measurements. PICES Special Publication 3, 191 pp.
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 710036
attribute NC_GLOBAL instruments_2_description String General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/134/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Water Temperature Sensor
attribute NC_GLOBAL instruments_2_instrument_nid String 647
attribute NC_GLOBAL instruments_2_supplied_name String Thermometer
attribute NC_GLOBAL instruments_3_acronym String pH Sensor
attribute NC_GLOBAL instruments_3_dataset_instrument_description String Specifically, spectrophotometric pH was determined as per SOP6. As described in Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to Best Practices for Ocean CO2 Measurements. PICES Special Publication 3, 191 pp.
attribute NC_GLOBAL instruments_3_dataset_instrument_nid String 710037
attribute NC_GLOBAL instruments_3_description String General term for an instrument that measures the pH or how acidic or basic a solution is.
attribute NC_GLOBAL instruments_3_instrument_name String pH Sensor
attribute NC_GLOBAL instruments_3_instrument_nid String 674
attribute NC_GLOBAL instruments_3_supplied_name String pH meter
attribute NC_GLOBAL instruments_4_dataset_instrument_description String Prior to each test, the shear flume was fitted on the settlement plate with clamps and the released spores were photographed using a microscope (Steindorff SXC, New York Microscopes) connected to a camera (Nikon Coolpix S3300), using the lines drawn in the working section for reference.
attribute NC_GLOBAL instruments_4_dataset_instrument_nid String 710039
attribute NC_GLOBAL instruments_4_description String Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".
attribute NC_GLOBAL instruments_4_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB05/ (external link)
attribute NC_GLOBAL instruments_4_instrument_name String Microscope-Optical
attribute NC_GLOBAL instruments_4_instrument_nid String 708
attribute NC_GLOBAL instruments_4_supplied_name String microscope (Steindorff SXC, New York Microscopes)
attribute NC_GLOBAL keywords String attached, bco, bco-dmo, biological, chemical, data, dataset, dmo, erddap, latitude, longitude, management, oceanography, office, preliminary, species, time, time2, treatment
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/710033/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/710033 (external link)
attribute NC_GLOBAL Northernmost_Northing double 48.51359
attribute NC_GLOBAL param_mapping String {'710033': {'lat': 'flag - latitude', 'lon': 'flag - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/710033/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Washington
attribute NC_GLOBAL people_0_affiliation_acronym String FHL
attribute NC_GLOBAL people_0_person_name String Emily Carrington
attribute NC_GLOBAL people_0_person_nid String 51609
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of British Columbia
attribute NC_GLOBAL people_1_affiliation_acronym String UBC-Bot
attribute NC_GLOBAL people_1_person_name String Patrick Martone
attribute NC_GLOBAL people_1_person_nid String 710046
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of British Columbia
attribute NC_GLOBAL people_2_affiliation_acronym String UBC-Bot
attribute NC_GLOBAL people_2_person_name String Rebecca Guenther
attribute NC_GLOBAL people_2_person_nid String 710051
attribute NC_GLOBAL people_2_role String Contact
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Mathew Biddle
attribute NC_GLOBAL people_3_person_nid String 708682
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String OA - Ecomaterials Perspective
attribute NC_GLOBAL projects_0_acronym String OA - Ecomaterials Perspective
attribute NC_GLOBAL projects_0_description String Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective
This award will support researchers based at the University of Washington's Friday Harbor Laboratories. The overall focus of the project is to determine how ocean acidification affects the integrity of biomaterials and how these effects in turn alter interactions among members of marine communities. The research plan emphasizes an ecomaterial approach; a team of biomaterials and ecomechanics experts will apply their unique perspective to detail how different combinations of environmental conditions affect the structural integrity and ecological performance of organisms. The study targets a diversity of ecologically important taxa, including bivalves, snails, crustaceans, and seaweeds, thereby providing insight into the range of possible biological responses to future changes in climate conditions. The proposal will enhance our understanding of the ecological consequences of climate change, a significant societal problem.
Each of the study systems has broader impacts in fields beyond ecomechanics. Engineers are particularly interested in biomaterials and in each system there are materials with commercial potential. The project will integrate research and education by supporting doctoral student dissertation research, providing undergraduate research opportunities via three training programs at FHL, and summer internships for talented high school students, recruited from the FHL Science Outreach Program. The participation of underrepresented groups will be broadened by actively recruiting URM and female students. Results will be disseminated in a variety of forums, including peer-reviewed scientific publications, undergraduate and graduate course material, service learning activities in K-8 classrooms, demonstrations at FHL's annual Open House, and columns for a popular science magazine.
attribute NC_GLOBAL projects_0_end_date String 2013-08
attribute NC_GLOBAL projects_0_geolocation String Friday Harbor, WA
attribute NC_GLOBAL projects_0_name String Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective
attribute NC_GLOBAL projects_0_project_nid String 2250
attribute NC_GLOBAL projects_0_project_website String http://depts.washington.edu/fhl/oael.html (external link)
attribute NC_GLOBAL projects_0_start_date String 2010-09
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 48.51359
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String latitude,longitude,location
attribute NC_GLOBAL summary String Pterosiphonia bipinnata and Corallina vancouveriensis initial experiment attachment and duration at Deadman Bay on San Juan Island, Washington
attribute NC_GLOBAL title String [Initial spores] - Pterosiphonia bipinnata and Corallina vancouveriensis initial experiment attachment and duration at Deadman Bay on San Juan Island, Washington (Effects of Ocean Acidification on Coastal Organisms: An Ecomaterials Perspective)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -123.14893
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable time2   double  
attribute time2 _FillValue double NaN
attribute time2 actual_range double 0.28, 48.83
attribute time2 bcodmo_name String duration
attribute time2 description String time elapsed
attribute time2 long_name String Time
attribute time2 units String hours
variable Attached   byte  
attribute Attached _FillValue byte 127
attribute Attached actual_range byte 0, 1
attribute Attached bcodmo_name String unknown
attribute Attached description String not attached (0) or attached (1)
attribute Attached long_name String Attached
attribute Attached units String unitless
variable Treatment   String  
attribute Treatment bcodmo_name String treatment
attribute Treatment description String High (7.8) or Low (7.3) pH conditions
attribute Treatment long_name String Treatment
attribute Treatment units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 48.51359, 48.51359
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String latitude with positive values indicating North.
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -123.14893, -123.14893
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String longitude with negative values indicating West
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable location   String  
attribute location bcodmo_name String site
attribute location description String name of the location
attribute location long_name String Location
attribute location units String unitless
variable Species   String  
attribute Species bcodmo_name String species
attribute Species description String Latin name of seaweed
attribute Species long_name String Species
attribute Species units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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