ERDDAP > info > bcodmo_dataset_712090

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
	set	data	graph		files	public	N isotopic composition of Phenylalanine and Glutamic Acid from a number of organisms, demonstrating new HPLC protocol for precise isotopic measurements		I   M	background			BCO-DMO	bcodmo_dataset_712090

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Methodology described in Broek & McCarthy (2014): AA standards Standard L-AA powders were purchased from Alfa Aesar and Acros Organics and used to prepare individual liquid standards (0.05 M), which were then combined as an equimolar mixture of 16 individual AAs (\"16 AA Standard\") for developing separations. The 16 AA Standard contained the proteinaceous AAs: glycine (Gly), L-alanine (Ala), L-arginine (Arg), L-aspartic acid (Asp), L-glutamic acid (Glu), L-histidine His), L-isoleucine (Ile), L-leucine (Leu), L-lysine (Lys), D/L-methionine (Met), L-phenylalanine (Phe), L-proline (Pro), L-serine (Ser), L-threonine (Thr), L-valine (Val); and nonprotein AA nor- leucine(Nle), which ;is commonly used as an internal standard (Popp et al. 2007; McCarthy et al. 2013). The \u03b415N and \u03b413C values for dry standards were determined by standard EA-IRMS at the University of California, Santa Cruz Stable Isotope Laboratory (UCSC-SIL) following standard protocols ([http://es.ucsc.edu/](\\"http://es.ucsc.edu/\\") ~ silab). Average precision of EA-IRMS \u03b415N standard values was 0.11 \u00b1 0.07 \u2030. Additionally, a commercially available equimolar AA standard mixture \"Pierce Amino Acid Standard H\" (Pierce H)(Thermo Scientific) containing the same AAs as the \"16 AA Standard\" with the exception of the nonprotein AA Nle and addition of the proteinaceous AAscysteine (Cys) and tyrosine (Tyr) was used to construct individual calibration curves, so as to verify relative molar abundance of individual AAs in natural samples. Sample preparation: The cyanobacteria sample (Spirulina Sp.) was obtained as a bulk commercial dry powder (Spirulina Pacifica, Nutrex Hawaii, Kailua-Kona, HI). This same sample has been used previously as a McCarthy laboratory internal quality control standard, and its CSI-AA values have been measured repeatedly by GC-C-IRMS, allowing an investigation of the long-term accuracy and precision of the GC-C-IRMS instrument. Coastal mussel (Myilitus Califorianus) sample was collected in 2012 from Santa Cruz, CA. The mussel was previously dissected, and the adductor muscle tissue removed and lyophilized prior to storage. We used a subsample of adductor muscle collected for a prior study (Vokhshoori and McCarthy 2014) hydrolyzing the bulk lyophilized adductor muscle tissue directly without lipid extraction. The deep-sea bamboo coral (genus isidella) sample was previously collected in 2007 from Monterey Bay, CA, USA (36 44.6538N, 122 2.2329W, 870.2 m) (Hill, pers. comm. 2011). A proteinaceous node was separated from the calcium carbonate skeleton and oven dried (60 degrees C, 24 h). White sea bass muscle tissue was subsampled from an incidental recreational catch in 2007, landed from Santa Cruz Island, Channel Islands, CA (J. Patterson, pers. comm. 2007). Fish muscle tissue was also lyophilized prior to hydrolysis. Harbor seal blood was collected in May-June 2007 from a wild animal in Tomales Bay, CA (38 13.9N, 122 58.1W) under NMFS Research Permit no. 555-1565. Blood serum was purified, lipid extracted, and lyophilized prior to hydrolysis, as described previously (Germain et al. 2011). For all sample types, proteinaceous material was hydrolyzed by adding 40-50 mg of bulk dry sample to an 8 mL glass vial, followed by 5 mL of 6 N hydrochloric acid (HCl) at room temperature. The vials were flushed with nitrogen gas, sealed, and allowed to hydrolyze under standard conditions (110 degrees C, 20 h). Hydrolysis under acidic conditions quantitatively deaminates asparagine (Asn) to aspartic acid, and glutamine (Gln) to glutamic acid (Barrett 1985). Therefore, in this protocol (and all others based on acid hydrolysis), measured Glu in fact represents Gln+Glu, and measured Asp represents Asp+Asn. We note that while the abbreviations Glx and Asx are sometimes used to denote these combined Gln+Glu and Asp+Asn fractions, we have elected to simply use Asp and Glu as abbreviations, as defined above, in order to correspond better with prior TPCSIA literature. Additionally, acid hydrolysis is known to destroy cysteine (Cys), precluding it from analysis (Barrett 1985). Resulting hydrolysates were dried to completion under nitrogen gas and brought up in 0.1 N HCl to a final concentration of 1 mg tissue/ 100 L HCl. Approximately 75% of each of the resulting mixtures was reserved for HPLC/EA-IRMS analysis, and the remaining material was dried to completion for derivatization and subsequent GC-C-IRMS analysis. GC-C-IRMS Analysis\u2028: Trifluoroacetyl isopropyl ester (TFA-IP) AA derivatives were prepared using standardized lab protocols, as described previously (McCarthy et al. 2013). Briefly, hydrolyzed samples were esterified in 300 uL 1:5 mixture of acetyl chloride:2-propanol (110 degrees C, 60 minutes). The resulting amino acid isopropyl esters were then acylated in 350 uL 1:3 mixture of dichloromethane (DCM):trifluoroacetic acid anhydride (100 degrees C, 15 minutes). Derivatized AAs were dissolved in DCM to a final ratio of 1 mg of original proteinaceous material to 50 uL DCM. Isotopic analysis was conducted on a Thermo Trace GC Ultra (Thermo Fisher Scientific, West Palm Beach, FL, USA) coupled via a Thermo GC IsoLink to a ThermoFinnigan DeltaPlus XP isotope ratio monitoring mass spectrometer (Thermo Fisher Scientific).\u00a0Derivatives (1 L) were injected (injector temp. 250 degrees C constant) onto an Agilent DB-5 column (50 m x 0.32 mm ID x 0.52 um film thickness, Agilent Technologies, Inc., Santa Clara, CA, USA), with a He carrier flow rate of 2 mL/min (constant-flow). Separations were achieved with a four-ramp oven program: 52 deg C, 2 min hold; ramp 1 = 15 deg C /min to 75 deg C, hold for 2 min; ramp 2 = 4 deg C /min to 185 deg C, hold for 2 min; ramp 3 = 4 deg C /min to 200 deg C; ramp 4 = 30 deg C /min to 240 deg C, hold for 5 min. This method allows for the determination of 11-15 AAs depending on derivatization efficiency and instrument sensitivity. Values are typically obtained for Gly, Ala, Glu, Ile, Leu, Phe, Pro, Ser, Thr, Val, Nle, and Lys.\u00a0Values for Met, His and Arg are obtained only in some samples, depending on concentration and derivatization efficiency. For \u03b415N AA values, samples were analyzed in quadruplicate (n=4) with bracketed lab AA isotopic standard mix for subsequent standard offset and drift corrections. Corrections based on authentic external standards were applied using previously published protocols (McCarthy et al. 2013).\u2028 HPLC/EA-IRMS: Liquid chromatographic separations were conducted using a Shimadzu HPLC system (Shimadzu Scientific Instruments, Inc., Columbia, MD, USA) equipped with: system controller (SCL-10A vp), degasser (DGU-20A5), 2 pumps (LC-20AD), autosampler (SIL-20A) with an adjustable injection volume of 0.1-100 uL, and coupled to a Shimadzu automated fraction collector (FRC-20A). An adjustable flow splitter (Analytical Sales and Services, Inc., Pompton Plains, NJ, USA) was used inline following the chromatography column to direct ~15% of the flow to a SEDERE (Alfortville, France) evaporative light scattering detector (ELSD- LT II, Sedex 85LT) for peak detection and quantitation. A semi-preparative scale SiELC Primesep A column (10 x 250 mm, 100 angstrom pore size, 5 um particle size; SiELC Technologies Ltd., Prospect Heights, IL, USA) was used for amino acid purification. The Primesep A column used here is a reverse- phase semi-preparative scale column embedded with strong acidic ion-pairing groups. Such mixed phase columns have been developed specifically for the separation of charged organic compounds as the acidic sites in the stationary phase interact with the charged functional groups and provide additional retention mechanisms to increase chromatographic separation potential. For a more detailed description of the retention mechanisms of the Primesep A column see (McCullagh et al. 2006; 2010).\u2028 Typically, 75-100 uL of sample solution was loaded onto the HPLC instrument. A binary solvent ramp program was used consisting of 0.1% trifluoroacetic acid (TFA) in HPLC grade water (aqueous phase) and 0.1% TFA in acetonitrile (organic phase). The final solvent ramp program used for optimal separation was as follows: starting with 100% aqueous / 0% organic; increased from 0 to 0.5% organic from 0-30 minutes; increased to 15% organic from 30-35 minutes; increased to 22.5% from 35-70 minutes; increased to 30% from 70-95 minutes; held at 30% until 140 minutes. The column was then cleaned and equilibrated by increasing to 100% and holding for 20 minutes; then decreasing to 50% and holding for an additional 15 minutes; then decreasing to 0% and holding until the method ends at 180 minutes. A flow rate ramp is also employed in which the total flow rate is held at 2.5 mL/minute for 0-30 minutes; increased to 4.5 mL/minute from 30-35 minutes; held at 4.5 mL/min from 35-170 minutes; then decreased back to 2.5 mL/minute from 170-175 minutes and held until the completion of the analysis.\u2028 Purified AAs were collected into 3.5 mL tubes via the automated fraction collector using time-based collections, and then transferred to 20 mL glass vials. The solvent was removed under vacuum using a Jouan centrifugal evaporator (Societe Jouan, Saint-Herblain, France) at a chamber temperature of 60 degrees C. Dry AA residues were then re-dissolved into a small volume (~30 L) of 0.1 N HCl, transferred into pre-ashed tin (Sn) EA capsules, and dried to completion in a 60 degrees C oven for 12 hours. Capsules were then pressed into cubes and analyzed for \u03b415N and \u03b413C values by EA-IRMS. EA-IRMS analysis was conducted in the UCSC shared Stable Isotope Laboratory facility (UCSC-SIL), using an EA-IRMS analyzer dedicated to smaller samples. This system uses a Carlo Erba CHNS-O EA1108-Elemental Analyzer, interfaced via a Thermo Finnigan Gasbench II device to a Thermo Finnigan Delta Plus XP isotope ratio mass spectrometer (Thermo Fisher Scientific), configured after Polissar et al. (2009). For AAs in this study, we found that \u2264 100 nmol quantities of purified AA material could be routinely measured using this instrument, although as discussed below a standard EA configuration could also equally be used. Raw EA-IRMS \u03b415N and \u03b413C values were corrected for instrument drift and size effects using AA isotopic standards and standard correction protocols used by the UCSC-SIL ([http://es.ucsc.edu/~silab](\\"http://es.ucsc.edu/~silab\\")).\u2028
attribute	NC_GLOBAL	awards_0_award_nid	String	704683
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1131816
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1131816
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Candace O. Major
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	51690
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Relative Amino Acid Abundance (mol %) HPLC Method Glu and Phe 15N values PI: Matthew McCarthy (UCSC) Version: 02 August 2017
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2017-08-04T17:02:19Z
attribute	NC_GLOBAL	date_modified	String	2019-08-02T15:50:09Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.712090.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/712090
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	IR Mass Spec
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Isotopic analysis was conducted on a Thermo Trace GC Ultra (Thermo Fisher Scientific, West Palm Beach, FL, USA) coupled via a Thermo GC IsoLink to a ThermoFinnigan DeltaPlus XP isotope ratio monitoring mass spectrometer (Thermo Fisher Scientific).
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	712097
attribute	NC_GLOBAL	instruments_0_description	String	The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB16/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Isotope-ratio Mass Spectrometer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	469
attribute	NC_GLOBAL	instruments_0_supplied_name	String	ThermoFinnigan DeltaPlus XP isotope ratio monitoring mass spectrometer
attribute	NC_GLOBAL	instruments_1_acronym	String	HPLC
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Liquid chromatographic separations were conducted using a Shimadzu HPLC system (Shimadzu Scientific Instruments, Inc., Columbia, MD, USA) equipped with: system controller (SCL-10A vp), degasser (DGU-20A5), 2 pumps (LC-20AD), autosampler (SIL-20A) with an adjustable injection volume of 0.1-100 μL, and coupled to a Shimadzu automated fraction collector (FRC-20A).
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	712098
attribute	NC_GLOBAL	instruments_1_description	String	A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB11/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	High Performance Liquid Chromatograph
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	506
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Shimadzu HPLC
attribute	NC_GLOBAL	instruments_2_acronym	String	Gas Chromatograph
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Derivatives (1 L) were injected (injector temp. 250 degrees C constant) onto an Agilent DB-5 column.
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	712099
attribute	NC_GLOBAL	instruments_2_description	String	Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)
attribute	NC_GLOBAL	instruments_2_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB02/
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Gas Chromatograph
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	661
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Agilent DB-5 column
attribute	NC_GLOBAL	instruments_3_acronym	String	Gas Chromatograph
attribute	NC_GLOBAL	instruments_3_dataset_instrument_description	String	Isotopic analysis was conducted on a Thermo Trace GC Ultra (Thermo Fisher Scientific, West Palm Beach, FL, USA) coupled via a Thermo GC IsoLink to a ThermoFinnigan DeltaPlus XP isotope ratio monitoring mass spectrometer (Thermo Fisher Scientific).
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	712096
attribute	NC_GLOBAL	instruments_3_description	String	Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)
attribute	NC_GLOBAL	instruments_3_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB02/
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Gas Chromatograph
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	661
attribute	NC_GLOBAL	instruments_3_supplied_name	String	Thermo Trace GC Ultra
attribute	NC_GLOBAL	instruments_4_dataset_instrument_description	String	EA-IRMS analysis was conducted in the UCSC shared Stable Isotope Laboratory facility (UCSC-SIL), using an EA-IRMS analyzer dedicated to smaller samples. This system uses a Carlo Erba CHNS-O EA1108-Elemental Analyzer, interfaced via a Thermo Finnigan Gasbench II device to a Thermo Finnigan Delta Plus XP isotope ratio mass spectrometer (Thermo Fisher Scientific).
attribute	NC_GLOBAL	instruments_4_dataset_instrument_nid	String	712095
attribute	NC_GLOBAL	instruments_4_description	String	Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material.
attribute	NC_GLOBAL	instruments_4_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB01/
attribute	NC_GLOBAL	instruments_4_instrument_name	String	Elemental Analyzer
attribute	NC_GLOBAL	instruments_4_instrument_nid	String	546339
attribute	NC_GLOBAL	instruments_4_supplied_name	String	Carlo Erba CHNS-O EA1108-elemental analyzer
attribute	NC_GLOBAL	keywords	String	acid, amino, amino_acid, bco, bco-dmo, biological, chemical, data, dataset, deviation, dmo, erddap, gcc, hplc, management, mol, mol_pcnt_GCC, mol_pcnt_GCC_stdev, mol_pcnt_hplc, mol_pcnt_hplc_stdev, oceanography, office, pcnt, preliminary, sample, sample_type, standard, standard deviation, stdev, type
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/712090/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/712090
attribute	NC_GLOBAL	param_mapping	String	{'712090': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/712090/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of California-Santa Cruz
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UC Santa Cruz
attribute	NC_GLOBAL	people_0_person_name	String	Matthew D. McCarthy
attribute	NC_GLOBAL	people_0_person_nid	String	557245
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Shannon Rauch
attribute	NC_GLOBAL	people_1_person_nid	String	51498
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	Amino Acid Sediment 15N
attribute	NC_GLOBAL	projects_0_acronym	String	Amino Acid Sediment 15N
attribute	NC_GLOBAL	projects_0_description	String	The bioavailability of nutrients plays a crucial role in oceanic biological productivity, the carbon cycle, and climate change. The global ocean inventory of nitrogen (N) is determined by the balance of N-fixation (sources) and denitrification (sinks). In this three-year project, a researcher from the University of California, Santa Cruz, will focus on developing compound-specific N isotope (d15N) analysis of amino acids as a new tool for understanding N source and transformation of organic matter in paleo-reservoirs. The offsets in the isotopic ratios of individual amino acid groups may yield information about trophic transfer, heterotrophic microbial reworking, and autotrophic versus heterotrophic sources. By measuring and comparing the bulk and amino acid d15N in size-fractioned samples from plankton tows, sediments traps, and multi-cores in oxic and suboxic depositional environments, the researcher will: (1) Provide a proxy of the d15N of average exported photoautotrophic organic matter; and (2) Provide a new level of detail into sedimentary organic N degradation and preservation. Broader impacts: This project will improve understanding of the fundamental underpinnings and behaviors of d15N amino acid patterns and how they behave in contrasting sedimentary environments, while also developing a potential paleoceanographic proxy. Funding will support a graduate student and undergraduate research at the institution. The researcher will also conduct community outreach in the form of a workshop/tutorial on the proxy development.
attribute	NC_GLOBAL	projects_0_end_date	String	2016-09
attribute	NC_GLOBAL	projects_0_geolocation	String	California Margin , Santa Barbara Basin , CA current system, Eastern Tropical Pacific
attribute	NC_GLOBAL	projects_0_name	String	The Use of Nitrogen Isotopes of Amino Acids To Understand Marine Sedimentary 15N Records
attribute	NC_GLOBAL	projects_0_project_nid	String	704684
attribute	NC_GLOBAL	projects_0_start_date	String	2011-10
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	subsetVariables	String	mol_pcnt_GCC_stdev
attribute	NC_GLOBAL	summary	String	N isotopic composition of Phenylalanine and Glutamic Acid from a number of organisms, demonstrating new HPLC protocol for precise isotopic measurements.
attribute	NC_GLOBAL	title	String	N isotopic composition of Phenylalanine and Glutamic Acid from a number of organisms, demonstrating new HPLC protocol for precise isotopic measurements
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	sample_type		String
attribute	sample_type	bcodmo_name	String	sample_type
attribute	sample_type	description	String	Description of the sample type.
attribute	sample_type	long_name	String	Sample Type
attribute	sample_type	units	String	unitless
variable	amino_acid		String
attribute	amino_acid	bcodmo_name	String	amino_acid
attribute	amino_acid	description	String	Amino acid
attribute	amino_acid	long_name	String	Amino Acid
attribute	amino_acid	units	String	unitless
variable	mol_pcnt_hplc		float
attribute	mol_pcnt_hplc	_FillValue	float	NaN
attribute	mol_pcnt_hplc	actual_range	float	1.63, 21.66
attribute	mol_pcnt_hplc	bcodmo_name	String	amino_conc
attribute	mol_pcnt_hplc	description	String	Relative amino acid abundance; determined by HPLC-ELSD. Average precision: +/- 1
attribute	mol_pcnt_hplc	long_name	String	Mol Pcnt Hplc
attribute	mol_pcnt_hplc	units	String	percent (%)
variable	mol_pcnt_hplc_stdev		float
attribute	mol_pcnt_hplc_stdev	_FillValue	float	NaN
attribute	mol_pcnt_hplc_stdev	actual_range	float	0.03, 0.63
attribute	mol_pcnt_hplc_stdev	bcodmo_name	String	amino_conc
attribute	mol_pcnt_hplc_stdev	description	String	Standard deviation of relative amino acid abundance determined by HPLC-ELSD.
attribute	mol_pcnt_hplc_stdev	long_name	String	Mol Pcnt Hplc Stdev
attribute	mol_pcnt_hplc_stdev	units	String	percent (%)
variable	mol_pcnt_GCC		float
attribute	mol_pcnt_GCC	_FillValue	float	NaN
attribute	mol_pcnt_GCC	actual_range	float	1.8, 18.55
attribute	mol_pcnt_GCC	bcodmo_name	String	amino_conc
attribute	mol_pcnt_GCC	description	String	Relative amino acid abundance; determined by GC-C-IRMS. Average precision: +/- 1
attribute	mol_pcnt_GCC	long_name	String	Mol Pcnt GCC
attribute	mol_pcnt_GCC	units	String	percent (%)
variable	mol_pcnt_GCC_stdev		double
attribute	mol_pcnt_GCC_stdev	_FillValue	double	NaN
attribute	mol_pcnt_GCC_stdev	bcodmo_name	String	amino_conc
attribute	mol_pcnt_GCC_stdev	description	String	Standard deviation of relative amino acid abundance determined by GC-C-IRMS.
attribute	mol_pcnt_GCC_stdev	long_name	String	Mol Pcnt GCC Stdev
attribute	mol_pcnt_GCC_stdev	units	String	percent (%)

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