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Title Sum-
Institution Dataset ID
     data   graph     files  public Single cell isotope incorporation of 1H, 2H, 12C14N, 12C15N. 12C12C, 12C13C from Chikyu-
337 (IODP 337)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String SIP Incubation Preparation
IODP Expedition 337 operations commenced July 26 and continued through
September 30, 2012 on the D/V Chikyu. Utilizing riser drilling, a sedimentary
sequence was recovered down to 2466 m below seafloor (mbsf) at Hole C0020A (41
10' 36\" N, 142 12' 02\" E) in 1180 m water depth off the Shimokita Peninsula.
The drilled sequence transitioned from open marine (youngest; late Pliocene,
~5 Ma) to terrestrial (oldest; late Oligocene, ~30 Ma) with depth. Models for
maximum temperature reached by Expedition 337 coring report 63.7 degrees C.
Shipboard sedimentological, geochemical, and microbiological data and methods
are available through IODP publications. Additional coal petrography is
available in Gross et al.\u00a0 A total of 52 incubation amendment conditions
were prepared onboard to interrogate a range of potential deep-biosphere
metabolic strategies, and then incubated back in the lab at temperatures
approximating that measured in situ. In this study, incubations from shale
(Core 8L4; 1606 mbsf; 37C incubation temperature), coal (Core 15R3; 1921 mbsf;
45C incubation temperature), and mixed (homogenized mixture from multiple
cores 19R1, 19R5, 19R7, 20R3, 23R6, 23R8, 24R3, 25R1, 25R2, and 25R3;
1950-1999 mbsf; 45C incubation temperature) with methanol and methylamine
substrate additions were analyzed. Age estimates of these samples are early to
middle Miocene. In situ temperatures ranged from 38C to 48C at these sample
depths with pressures ~30 MPa. Two coal beds were included in these
incubations: a shallower coal-only sample deposited under more marine-
influenced conditions (~1921 mbsf, core 15R3) and a deeper coal bed deposited
under more limnic conditions that was included in the mixed lithology sample
(~2000 mbsf, cores 24R3 and 25R1). Cores used for incubations were prepared by
removal of outer drill-fluid-contaminated layers by sterile ceramic knife as
soon as possible after core recovery and stored at 4C until incubation
preparation, while maintaining an anaerobic atmosphere during the entire
process.\u00a0For preparation of the SIP incubations, the interior portion of
the core was manually crushed into cm-sized pieces under sterile, anaerobic
conditions and distributed evenly into sterile 50 ml glass vials with butyl
rubber stoppers and screw caps (Nichidenrika-Glass Co. Ltd.).\u00a0 Vials were
flushed with argon and pressurized to 1 atm argon headspace. Sterile C-, N-,
and S-free media (1% PBS, 30 g/L NaCl, 12 g/L MgCl2, and 3 g/L KCl) was
prepared anaerobically with deuterated water (20 at. % 2H2O). 20 at. % 2H2O
was selected as the highest level of enrichment with little to no effect on
the activity of microorganisms in pure culture. Time point 1, time point 2,
and autoclaved treatments were prepared for each substrate condition. Time
point 1 incubations lasted for six months, while time point 2 and autoclaved
treatments were maintained at the in situ incubation temperature for 2.5
years. Due to low levels of activity ascertained from geochemical
measurements, all NanoSIMS analyses were conducted on time point 2 and
autoclaved samples. Amendments and incubation conditions for the methyl-
substrate subset analyzed in this study are provided in this
dataset.\u00a0Equimolar amounts of substrate (30 umol C, 1.5 mM final; 3 umol
N, 0.15 mM final) were added across incubation conditions at 50 at. %
(Cambridge Isotopes). Hydrogen was added as 5 mL 100% H2 overpressure to
incubations (~15% H2 headspace). A full list of the additional incubation
conditions prepared onboard are listed in cruise Methods. Alkalinity (34.39
\u2013 9.68 mM) ammonium (2.80 \u2013 1.83 mM) concentrations from formation
fluid samples collected onboard exceed concentrations of C and N amendments.
Concentrations of methylamine (0.05 mM) and methanol (1 mM) measured from
lignite coal also suggest our substrate additions were environmentally
relevant. After 30 months of incubation (March 2014) all treatments were
sampled for geochemical analyses prior to preparation for NanoSIMS.\u00a03 ml
of headspace gas was removed to a vial filled with 0.1 M NaOH for methane
analysis. About 1 ml of liquid was filtered through a 0.1 um 13 mm Whatman
Polycarbonate Nuclepore Track-Etched Membrane (110405) for DIC analysis. See
Supplemental Methods of Trembath-Reichert et al. for detailed description of
methane and DIC analyses.

Sample preparation for NanoSIMS analysis
To overcome technical challenges for NanoSIMS analysis of low biomass
samples, cell separation and fluorescence-activated cell sorting (FACS) were
used to directly concentrate cells in a small analysis area, ~1 to 0.5 sq.
mm.\u00a0NanoSIMS samples were prepared from paraformaldehyde (PFA)-fixed cell
separates after 894 days of incubation. Cell preservation, separation,
enumeration, and FACS were all conducted in the clean booth and clean room
facilities at Kochi Institute for Core Sample Research, JAMSTEC. Half of the
solid and half of the liquid portion of each sample were fixed overnight in a
solution of 2% paraformaldehyde (PFA), 3 \u00d7 phosphate buffered saline
(PBS).\u00a0Samples were then subjected to two washes, incubating in 3 \u00d7
PBS for 6 hrs and then 2 hrs, after each wash respectively. Samples were
centrifuged (3500 \u00d7 g) and supernatant was decanted after each wash. PFA-
fixed samples were stored in 50 % ethanol : 3 \u00d7 PBS. The other half of
the sample was preserved in glyTE (70% glycerol, 100mM Tris, 10mM EDTA;
Bigelow Single Cell Genomics Center preservation protocol) and frozen by cell
alive system (CAS) and stored at -80C. 1 ml liquid and ~1 g sediment chips
were subsampled by pipet and sterile cell culture loop, respectively, from the
PFA-fixed sample.\u00a0Cell separation, microscopy, and sorting procedures
followed Morono et al., with the following modifications: 1) samples were
sonicated (Bioruptor UCD-250, COSMO BIO) in an ice bath for 20 cycles of 30
sec 200 W, 30 sec off, and 2) samples were incubated in hydrofluoric acid post
initial sonication, rather than after first density gradient
separation.\u00a0Cell detection limit was determined by no-sample added
controls run in parallel with samples. Cells were stained with SYBR Green I
(1:40 dilution of SYBR Green in Tris (10 mM) \u2013EDTA (1mM) (TE) and sorted
following the flow cytometry protocol of Morono et al. Sorted cells were
concentrated directly from the sorter onto NanoSIMS compatible 0.2 um
polycarbonate filters coated with indium tin oxide (ITO) as described in
Morono et al.\u00a0and Inagaki et al. ITO coating on polycarbonate membranes
(Isopore GTBP02500 Millipore) was prepared by sputtering deposition technique
at Astellatech Co. Ltd. (Kanagawa, Japan).\u00a0Scanning electron microscopy
(SEM) of the filters was done on a Zeiss 1550 VP Field Emission Scanning
Electron Microscope at the GPS Division Analytical Facility at Caltech and
SYBR stained cells were imaged with a BX51 epifluorescence microscope
(Olympus, Tokyo, Japan) using 20\u00d7 (UPlanFL N) dry, 60\u00d7 (PlanApo N),
and 100\u00d7 (UPlanFL N) oil immersion objectives.
attribute NC_GLOBAL awards_0_award_nid String 554980
attribute NC_GLOBAL awards_0_award_number String OCE-0939564
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=0939564 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String NanoSIMS HCN - All Data
from IODP Expedition 337
PI: Elizabeth Trembath-Reichert
Version: 10 August 2017
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2017-08-10T15:46:58Z
attribute NC_GLOBAL date_modified String 2019-08-02T16:14:21Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.712652.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/712652 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_dataset_instrument_description String SYBR stained cells were imaged with a BX51 epifluorescence microscope (Olympus, Tokyo, Japan).
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 712677
attribute NC_GLOBAL instruments_0_description String Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB06/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Microscope-Fluorescence
attribute NC_GLOBAL instruments_0_instrument_nid String 695
attribute NC_GLOBAL instruments_0_supplied_name String BX51 epifluorescence microscope
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Cell targets were identified (by SYBR stain) and marked on NanoSIMS membranes with a laser dissection microscope (LMD6000; Leica Microsystems) for ease of rediscovery on the NanoSIMS.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 712675
attribute NC_GLOBAL instruments_1_description String Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB05/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Microscope-Optical
attribute NC_GLOBAL instruments_1_instrument_nid String 708
attribute NC_GLOBAL instruments_1_supplied_name String LMD6000
attribute NC_GLOBAL instruments_2_acronym String SEM
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Scanning electron microscopy (SEM) of the filters was done on a Zeiss 1550 VP Field Emission Scanning Electron Microscope at the GPS Division Analytical Facility at Caltech.
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 712676
attribute NC_GLOBAL instruments_2_description String Scanning electron microscope
attribute NC_GLOBAL instruments_2_instrument_name String Scanning Electron Microscope
attribute NC_GLOBAL instruments_2_instrument_nid String 637895
attribute NC_GLOBAL instruments_2_supplied_name String Zeiss 1550 VP Field Emission Scanning Electron Microscope
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, c12, C12_LWratio, C12_MEANi, C12_PIXELSi, C12_Poiss_Ei, C12_Poiss_pcnt_Ei, C12_SIZEi, c13, C13C12_LWratio, C13C12_MEANi, C13C12_PIXELSi, C13C12_Poiss_Ei, C13C12_Poiss_pcnt_Ei, C13C12_SIZEi, c13f, C13f_MEAN, C13R_MEAN, chemical, condition, data, dataset, dmo, erddap, file, file_name, H1_LWratio, H1_MEANi, H1_PIXELSi, H1_Poiss_Ei, H1_Poiss_pcnt_Ei, H1_SIZEi, H2_LWratio, H2_MEANi, H2_PIXELSi, H2_Poiss_Ei, H2_Poiss_pcnt_Ei, H2_SIZEi, h2f, H2f_MEAN, H2R_MEAN, lwratio, management, mean, meani, morph, n14, N14C12_LWratio, N14C12_MEANi, N14C12_PIXELSi, N14C12_Poiss_Ei, N14C12_Poiss_pcnt_Ei, N14C12_SIZEi, n15, N15C12_LWratio, N15C12_MEANi, N15C12_PIXELSi, N15C12_Poiss_Ei, N15C12_Poiss_pcnt_Ei, N15C12_SIZEi, n15f, N15f_MEAN, N15R_MEAN, name, oceanography, office, pcnt, pixelsi, poiss, preliminary, roi, sample, sizei, sum, SumC, SumH, SumN, xi
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/712652/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/712652 (external link)
attribute NC_GLOBAL param_mapping String {'712652': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/712652/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String California Institute of Technology
attribute NC_GLOBAL people_0_affiliation_acronym String Caltech
attribute NC_GLOBAL people_0_person_name String Elizabeth Trembath-Reichert
attribute NC_GLOBAL people_0_person_nid String 672596
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Shannon Rauch
attribute NC_GLOBAL people_1_person_nid String 51498
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String Deep biosphere cell activity
attribute NC_GLOBAL projects_0_acronym String Deep biosphere cell activity
attribute NC_GLOBAL projects_0_description String IODP Expedition 337 set the record for deepest marine scientific drilling down to 2.4 kmbsf. This cruise also had the unique opportunity to retrieve deep cores from the Shimokita coal bed system in Japan with the aseptic and anaerobic conditions necessary to look for deep life. Onboard scientists prepared nearly 1,700 microbiology samples shared among five different countries to study life in the deep biosphere. Samples spanned over 1km in sampling depths and include representatives of shale, sandstone, and coal lithologies. Findings from previous IODP and deep mine expeditions suggest the genetic potential for methylotrophy in the deep subsurface, but it has yet to be observed in incubations. A subset of Expedition 337 anoxic incubations were prepared with a range of 13C-methyl substrates (methane, methylamine, and methanol) and maintained near in situ temperatures. To observe 13C methyl compound metabolism over time, we monitored the δ13C of the dissolved inorganic carbon and methane (by-products of methyl compound metabolism) over a period of 1.5 years. Our geochemical evidence suggests that the coal horizon incubated with 13C-methylamine showed the highest activity of all methyl incubations. Therefore, there are not only cells in the deeply buried terrigenous coal bed at Shimokita, but a microbial community that can be activated by methylotrophic compounds. Incubations showing the highest geochemical activity were prepared at the JAMSTEC Kochi Core Center for nanoSIMS analysis in March of 2015, and will be analyzed at Caltech in the coming months. This will allow us to observe if cells also incorporated the labeled methyl compounds into their body mass and provide another line of evidence that these substrates were used by the deep coalbed microbial community.
attribute NC_GLOBAL projects_0_end_date String 2015-04
attribute NC_GLOBAL projects_0_geolocation String Hole C0020A (41°10′36″N, 142°12′02″E) Shimokita Peninsula.
attribute NC_GLOBAL projects_0_name String Determination of deep biosphere cell activity and identity utilizing the state of the art low-biomass, single cell techniques developed at JAMSTEC in their class 10,000 clean room
attribute NC_GLOBAL projects_0_project_nid String 672592
attribute NC_GLOBAL projects_0_project_website String http://www.darkenergybiosphere.org/award/determination-of-deep-biosphere-cell-activity-and-identity-utilizing-the-state-of-the-art-low-biomass-single-cell-techniques-developed-at-jamstec-in-their-class-10000-clean-room/ (external link)
attribute NC_GLOBAL projects_0_start_date String 2015-03
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Single cell isotope incorporation of 1H, 2H, 12C14N, 12C15N. 12C12C, 12C13C ions from samples collected on IODP Expedition 337.
attribute NC_GLOBAL title String Single cell isotope incorporation of 1H, 2H, 12C14N, 12C15N. 12C12C, 12C13C from Chikyu-337 (IODP 337)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable file_name   String  
attribute file_name bcodmo_name String file_name
attribute file_name description String Name of raw nanosims frame
attribute file_name long_name String File Name
attribute file_name units String unitless
variable Sample   short  
attribute Sample _FillValue short 32767
attribute Sample actual_range short 6056, 6230
attribute Sample bcodmo_name String sample
attribute Sample description String Sample identifier
attribute Sample long_name String Sample
attribute Sample nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Sample units String unitless
variable Condition   String  
attribute Condition bcodmo_name String sample_descrip
attribute Condition description String Identifies amendments
attribute Condition long_name String Condition
attribute Condition units String unitless
variable ROI   byte  
attribute ROI _FillValue byte 127
attribute ROI actual_range byte 0, 69
attribute ROI bcodmo_name String sample_descrip
attribute ROI description String Region of interest; each file_name will have multiple regions of interest (ROIs), which is the single cell data
attribute ROI long_name String ROI
attribute ROI units String unitless
variable Morph   String  
attribute Morph bcodmo_name String sample_descrip
attribute Morph description String Morphology of the sample
attribute Morph long_name String Morph
attribute Morph units String unitless
variable Xi   float  
attribute Xi _FillValue float NaN
attribute Xi actual_range float 0.0, 505.2
attribute Xi bcodmo_name String unknown
attribute Xi description String x-coordinate where the ROI is in the image frame
attribute Xi long_name String Xi
attribute Xi units String microns (or pixels, if specified)
variable Yi   float  
attribute Yi _FillValue float NaN
attribute Yi actual_range float 0.0, 502.61
attribute Yi bcodmo_name String unknown
attribute Yi description String y-coordinate where the ROI is in the image frame
attribute Yi long_name String Yi
attribute Yi units String microns (or pixels, if specified)
variable H1_MEANi   int  
attribute H1_MEANi _FillValue int 2147483647
attribute H1_MEANi actual_range int 0, 17990659
attribute H1_MEANi bcodmo_name String count
attribute H1_MEANi description String 1H Mean ion counts
attribute H1_MEANi long_name String H1 MEANi
attribute H1_MEANi units String unitless
variable H1_Poiss_Ei   float  
attribute H1_Poiss_Ei _FillValue float NaN
attribute H1_Poiss_Ei actual_range float 0.0, 4241.54
attribute H1_Poiss_Ei bcodmo_name String unknown
attribute H1_Poiss_Ei description String 1H Poisson standard error
attribute H1_Poiss_Ei long_name String H1 Poiss Ei
attribute H1_Poiss_Ei units String unitless
variable H1_Poiss_pcnt_Ei   float  
attribute H1_Poiss_pcnt_Ei _FillValue float NaN
attribute H1_Poiss_pcnt_Ei actual_range float 0.0, 17.15
attribute H1_Poiss_pcnt_Ei bcodmo_name String unknown
attribute H1_Poiss_pcnt_Ei description String 1H Poisson percentage
attribute H1_Poiss_pcnt_Ei long_name String H1 Poiss Pcnt Ei
attribute H1_Poiss_pcnt_Ei units String unitless
variable H1_SIZEi   float  
attribute H1_SIZEi _FillValue float NaN
attribute H1_SIZEi actual_range float 0.0, 22.34
attribute H1_SIZEi bcodmo_name String unknown
attribute H1_SIZEi description String 1H Size of ROI
attribute H1_SIZEi long_name String H1 SIZEi
attribute H1_SIZEi units String microns (or pixels, if specified)
variable H1_PIXELSi   int  
attribute H1_PIXELSi _FillValue int 2147483647
attribute H1_PIXELSi actual_range int 0, 256910
attribute H1_PIXELSi bcodmo_name String unknown
attribute H1_PIXELSi description String 1H Number of pixels in ROI
attribute H1_PIXELSi long_name String H1 PIXELSi
attribute H1_PIXELSi units String unitless
variable H1_LWratio   float  
attribute H1_LWratio _FillValue float NaN
attribute H1_LWratio actual_range float 0.0, 8.02
attribute H1_LWratio bcodmo_name String unknown
attribute H1_LWratio description String 1H Length to width ratio of ROI
attribute H1_LWratio long_name String H1 LWratio
attribute H1_LWratio units String unitless
variable H2_MEANi   int  
attribute H2_MEANi _FillValue int 2147483647
attribute H2_MEANi actual_range int 0, 92693
attribute H2_MEANi bcodmo_name String count
attribute H2_MEANi description String 2H Mean ion counts
attribute H2_MEANi long_name String H2 MEANi
attribute H2_MEANi units String unitless
variable H2_Poiss_Ei   float  
attribute H2_Poiss_Ei _FillValue float NaN
attribute H2_Poiss_Ei actual_range float 0.0, 304.46
attribute H2_Poiss_Ei bcodmo_name String unknown
attribute H2_Poiss_Ei description String 2H Poisson standard error
attribute H2_Poiss_Ei long_name String H2 Poiss Ei
attribute H2_Poiss_Ei units String unitless
variable H2_Poiss_pcnt_Ei   float  
attribute H2_Poiss_pcnt_Ei _FillValue float NaN
attribute H2_Poiss_pcnt_Ei actual_range float 0.0, 100.0
attribute H2_Poiss_pcnt_Ei bcodmo_name String unknown
attribute H2_Poiss_pcnt_Ei description String 2H Poisson percentage
attribute H2_Poiss_pcnt_Ei long_name String H2 Poiss Pcnt Ei
attribute H2_Poiss_pcnt_Ei units String unitless
variable H2_SIZEi   float  
attribute H2_SIZEi _FillValue float NaN
attribute H2_SIZEi actual_range float 0.0, 22.34
attribute H2_SIZEi bcodmo_name String unknown
attribute H2_SIZEi description String 2H Size of ROI
attribute H2_SIZEi long_name String H2 SIZEi
attribute H2_SIZEi units String microns (or pixels, if specified)
variable H2_PIXELSi   int  
attribute H2_PIXELSi _FillValue int 2147483647
attribute H2_PIXELSi actual_range int 0, 256910
attribute H2_PIXELSi bcodmo_name String unknown
attribute H2_PIXELSi description String 2H Number of pixels in ROI
attribute H2_PIXELSi long_name String H2 PIXELSi
attribute H2_PIXELSi units String unitless
variable H2_LWratio   float  
attribute H2_LWratio _FillValue float NaN
attribute H2_LWratio actual_range float 0.0, 8.02
attribute H2_LWratio bcodmo_name String unknown
attribute H2_LWratio description String 2H Length to width ratio of ROI
attribute H2_LWratio long_name String H2 LWratio
attribute H2_LWratio units String unitless
variable H2R_MEAN   double  
attribute H2R_MEAN _FillValue double NaN
attribute H2R_MEAN actual_range double 0.0, 0.292050012
attribute H2R_MEAN bcodmo_name String unknown
attribute H2R_MEAN description String Mean ratio of minor ion to major ion
attribute H2R_MEAN long_name String H2 R MEAN
attribute H2R_MEAN units String unitless
variable H2f_MEAN   double  
attribute H2f_MEAN _FillValue double NaN
attribute H2f_MEAN actual_range double 0.0, 0.226036151
attribute H2f_MEAN bcodmo_name String unknown
attribute H2f_MEAN description String Fractional abundance of minor ion to major ion
attribute H2f_MEAN long_name String H2f MEAN
attribute H2f_MEAN units String unitless
variable C12_MEANi   int  
attribute C12_MEANi _FillValue int 2147483647
attribute C12_MEANi actual_range int 0, 62579745
attribute C12_MEANi bcodmo_name String count
attribute C12_MEANi description String 12C2 Mean ion counts
attribute C12_MEANi long_name String C12 MEANi
attribute C12_MEANi units String unitless
variable C12_Poiss_Ei   float  
attribute C12_Poiss_Ei _FillValue float NaN
attribute C12_Poiss_Ei actual_range float 0.0, 7910.74
attribute C12_Poiss_Ei bcodmo_name String unknown
attribute C12_Poiss_Ei description String 12C2 Poisson standard error
attribute C12_Poiss_Ei long_name String C12 Poiss Ei
attribute C12_Poiss_Ei units String unitless
variable C12_Poiss_pcnt_Ei   float  
attribute C12_Poiss_pcnt_Ei _FillValue float NaN
attribute C12_Poiss_pcnt_Ei actual_range float 0.0, 7.25
attribute C12_Poiss_pcnt_Ei bcodmo_name String unknown
attribute C12_Poiss_pcnt_Ei description String 12C2 Poisson percentage
attribute C12_Poiss_pcnt_Ei long_name String C12 Poiss Pcnt Ei
attribute C12_Poiss_pcnt_Ei units String unitless
variable C12_SIZEi   float  
attribute C12_SIZEi _FillValue float NaN
attribute C12_SIZEi actual_range float 0.0, 22.34
attribute C12_SIZEi bcodmo_name String unknown
attribute C12_SIZEi description String 12C2 Size of ROI
attribute C12_SIZEi long_name String C12 SIZEi
attribute C12_SIZEi units String microns (or pixels, if specified)
variable C12_PIXELSi   int  
attribute C12_PIXELSi _FillValue int 2147483647
attribute C12_PIXELSi actual_range int 0, 256910
attribute C12_PIXELSi bcodmo_name String unknown
attribute C12_PIXELSi description String 12C2 Number of pixels in ROI
attribute C12_PIXELSi long_name String C12 PIXELSi
attribute C12_PIXELSi units String unitless
variable C12_LWratio   float  
attribute C12_LWratio _FillValue float NaN
attribute C12_LWratio actual_range float 0.0, 8.02
attribute C12_LWratio bcodmo_name String unknown
attribute C12_LWratio description String 12C2 Length to width ratio of ROI
attribute C12_LWratio long_name String C12 LWratio
attribute C12_LWratio units String unitless
variable C13C12_MEANi   int  
attribute C13C12_MEANi _FillValue int 2147483647
attribute C13C12_MEANi actual_range int 0, 1180583
attribute C13C12_MEANi bcodmo_name String count
attribute C13C12_MEANi description String 13C12C Mean ion counts
attribute C13C12_MEANi long_name String C13 C12 MEANi
attribute C13C12_MEANi units String unitless
variable C13C12_Poiss_Ei   float  
attribute C13C12_Poiss_Ei _FillValue float NaN
attribute C13C12_Poiss_Ei actual_range float 0.0, 1086.55
attribute C13C12_Poiss_Ei bcodmo_name String unknown
attribute C13C12_Poiss_Ei description String 13C12C Poisson standard error
attribute C13C12_Poiss_Ei long_name String C13 C12 Poiss Ei
attribute C13C12_Poiss_Ei units String unitless
variable C13C12_Poiss_pcnt_Ei   float  
attribute C13C12_Poiss_pcnt_Ei _FillValue float NaN
attribute C13C12_Poiss_pcnt_Ei actual_range float 0.0, 40.82
attribute C13C12_Poiss_pcnt_Ei bcodmo_name String unknown
attribute C13C12_Poiss_pcnt_Ei description String 13C12C Poisson percentage
attribute C13C12_Poiss_pcnt_Ei long_name String C13 C12 Poiss Pcnt Ei
attribute C13C12_Poiss_pcnt_Ei units String unitless
variable C13C12_SIZEi   float  
attribute C13C12_SIZEi _FillValue float NaN
attribute C13C12_SIZEi actual_range float 0.0, 22.34
attribute C13C12_SIZEi bcodmo_name String unknown
attribute C13C12_SIZEi description String 13C12C Size of ROI
attribute C13C12_SIZEi long_name String C13 C12 SIZEi
attribute C13C12_SIZEi units String microns (or pixels, if specified)
variable C13C12_PIXELSi   int  
attribute C13C12_PIXELSi _FillValue int 2147483647
attribute C13C12_PIXELSi actual_range int 0, 256910
attribute C13C12_PIXELSi bcodmo_name String unknown
attribute C13C12_PIXELSi description String 13C12C Number of pixels in ROI
attribute C13C12_PIXELSi long_name String C13 C12 PIXELSi
attribute C13C12_PIXELSi units String unitless
variable C13C12_LWratio   float  
attribute C13C12_LWratio _FillValue float NaN
attribute C13C12_LWratio actual_range float 0.0, 8.02
attribute C13C12_LWratio bcodmo_name String unknown
attribute C13C12_LWratio description String 13C12C Length to width ratio of ROI
attribute C13C12_LWratio long_name String C13 C12 LWratio
attribute C13C12_LWratio units String unitless
variable C13R_MEAN   double  
attribute C13R_MEAN _FillValue double NaN
attribute C13R_MEAN actual_range double 0.0, 0.054152446
attribute C13R_MEAN bcodmo_name String unknown
attribute C13R_MEAN description String mean ratio of minor ion to major ion
attribute C13R_MEAN long_name String C13 R MEAN
attribute C13R_MEAN units String unitless
variable C13f_MEAN   double  
attribute C13f_MEAN _FillValue double NaN
attribute C13f_MEAN actual_range double 0.0, 0.051370602
attribute C13f_MEAN bcodmo_name String unknown
attribute C13f_MEAN description String fractional abundance of minor ion to major ion
attribute C13f_MEAN long_name String C13f MEAN
attribute C13f_MEAN units String unitless
variable N14C12_MEANi   int  
attribute N14C12_MEANi _FillValue int 2147483647
attribute N14C12_MEANi actual_range int 0, 175861356
attribute N14C12_MEANi bcodmo_name String count
attribute N14C12_MEANi description String 14N12C Mean ion counts
attribute N14C12_MEANi long_name String N14 C12 MEANi
attribute N14C12_MEANi units String unitless
variable N14C12_Poiss_Ei   float  
attribute N14C12_Poiss_Ei _FillValue float NaN
attribute N14C12_Poiss_Ei actual_range float 0.0, 13261.27
attribute N14C12_Poiss_Ei bcodmo_name String unknown
attribute N14C12_Poiss_Ei description String 14N12C Poisson standard error
attribute N14C12_Poiss_Ei long_name String N14 C12 Poiss Ei
attribute N14C12_Poiss_Ei units String unitless
variable N14C12_Poiss_pcnt_Ei   float  
attribute N14C12_Poiss_pcnt_Ei _FillValue float NaN
attribute N14C12_Poiss_pcnt_Ei actual_range float 0.0, 2.82
attribute N14C12_Poiss_pcnt_Ei bcodmo_name String unknown
attribute N14C12_Poiss_pcnt_Ei description String 14N12C Poisson percentage
attribute N14C12_Poiss_pcnt_Ei long_name String N14 C12 Poiss Pcnt Ei
attribute N14C12_Poiss_pcnt_Ei units String unitless
variable N14C12_SIZEi   float  
attribute N14C12_SIZEi _FillValue float NaN
attribute N14C12_SIZEi actual_range float 0.0, 22.34
attribute N14C12_SIZEi bcodmo_name String unknown
attribute N14C12_SIZEi description String 14N12C Size of ROI
attribute N14C12_SIZEi long_name String N14 C12 SIZEi
attribute N14C12_SIZEi units String microns (or pixels, if specified)
variable N14C12_PIXELSi   int  
attribute N14C12_PIXELSi _FillValue int 2147483647
attribute N14C12_PIXELSi actual_range int 0, 256910
attribute N14C12_PIXELSi bcodmo_name String unknown
attribute N14C12_PIXELSi description String 14N12C Number of pixels in ROI
attribute N14C12_PIXELSi long_name String N14 C12 PIXELSi
attribute N14C12_PIXELSi units String unitless
variable N14C12_LWratio   float  
attribute N14C12_LWratio _FillValue float NaN
attribute N14C12_LWratio actual_range float 0.0, 8.02
attribute N14C12_LWratio bcodmo_name String unknown
attribute N14C12_LWratio description String 14N12C Length to width ratio of ROI
attribute N14C12_LWratio long_name String N14 C12 LWratio
attribute N14C12_LWratio units String unitless
variable N15C12_MEANi   int  
attribute N15C12_MEANi _FillValue int 2147483647
attribute N15C12_MEANi actual_range int 0, 2349277
attribute N15C12_MEANi bcodmo_name String unknown
attribute N15C12_MEANi description String 15N12C Mean ion counts
attribute N15C12_MEANi long_name String N15 C12 MEANi
attribute N15C12_MEANi units String unitless
variable N15C12_Poiss_Ei   float  
attribute N15C12_Poiss_Ei _FillValue float NaN
attribute N15C12_Poiss_Ei actual_range float 0.0, 1532.74
attribute N15C12_Poiss_Ei bcodmo_name String unknown
attribute N15C12_Poiss_Ei description String 15N12C Poisson standard error
attribute N15C12_Poiss_Ei long_name String N15 C12 Poiss Ei
attribute N15C12_Poiss_Ei units String unitless
variable N15C12_Poiss_pcnt_Ei   float  
attribute N15C12_Poiss_pcnt_Ei _FillValue float NaN
attribute N15C12_Poiss_pcnt_Ei actual_range float 0.0, 40.82
attribute N15C12_Poiss_pcnt_Ei bcodmo_name String unknown
attribute N15C12_Poiss_pcnt_Ei description String 15N12C Poisson percentage
attribute N15C12_Poiss_pcnt_Ei long_name String N15 C12 Poiss Pcnt Ei
attribute N15C12_Poiss_pcnt_Ei units String unitless
variable N15C12_SIZEi   float  
attribute N15C12_SIZEi _FillValue float NaN
attribute N15C12_SIZEi actual_range float 0.0, 22.34
attribute N15C12_SIZEi bcodmo_name String unknown
attribute N15C12_SIZEi description String 15N12C Size of ROI
attribute N15C12_SIZEi long_name String N15 C12 SIZEi
attribute N15C12_SIZEi units String microns (or pixels, if specified)
variable N15C12_PIXELSi   int  
attribute N15C12_PIXELSi _FillValue int 2147483647
attribute N15C12_PIXELSi actual_range int 0, 256910
attribute N15C12_PIXELSi bcodmo_name String unknown
attribute N15C12_PIXELSi description String 15N12C Number of pixels in ROI
attribute N15C12_PIXELSi long_name String N15 C12 PIXELSi
attribute N15C12_PIXELSi units String unitless
variable N15C12_LWratio   float  
attribute N15C12_LWratio _FillValue float NaN
attribute N15C12_LWratio actual_range float 0.0, 8.02
attribute N15C12_LWratio bcodmo_name String unknown
attribute N15C12_LWratio description String 15N12C Length to width ratio of ROI
attribute N15C12_LWratio long_name String N15 C12 LWratio
attribute N15C12_LWratio units String unitless
variable N15R_MEAN   double  
attribute N15R_MEAN _FillValue double NaN
attribute N15R_MEAN actual_range double 0.0, 0.073149187
attribute N15R_MEAN bcodmo_name String unknown
attribute N15R_MEAN description String mean ratio of minor ion to major ion
attribute N15R_MEAN long_name String N15 R MEAN
attribute N15R_MEAN units String unitless
variable N15f_MEAN   double  
attribute N15f_MEAN _FillValue double NaN
attribute N15f_MEAN actual_range double 0.0, 0.068163111
attribute N15f_MEAN bcodmo_name String unknown
attribute N15f_MEAN description String fractional abundance of minor ion to major ion
attribute N15f_MEAN long_name String N15f MEAN
attribute N15f_MEAN units String unitless
variable SumH   int  
attribute SumH _FillValue int 2147483647
attribute SumH actual_range int 0, 17993307
attribute SumH bcodmo_name String count
attribute SumH description String Sum of all ion counts across both ions of hydrogen
attribute SumH long_name String Sum H
attribute SumH units String unitless
variable SumC   int  
attribute SumC _FillValue int 2147483647
attribute SumC actual_range int 0, 63760328
attribute SumC bcodmo_name String count
attribute SumC description String Sum of all ion counts across all ions of carbon
attribute SumC long_name String Sum C
attribute SumC units String unitless
variable SumN   int  
attribute SumN _FillValue int 2147483647
attribute SumN actual_range int 0, 176515752
attribute SumN bcodmo_name String count
attribute SumN description String Sum of all ion counts across all ions of nitrogen
attribute SumN long_name String Sum N
attribute SumN units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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