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     data   graph     files  public Diel series of pico-cyanobacteria concentration and cell properties from the RV Cape Hatteras
cruises CH0409 and CH0510 in the Western Sargasso Sea in 2009 and 2010.
   ?     I   M   background (external link) RSS BCO-DMO bcodmo_dataset_716814

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson,.odvTxt
attribute NC_GLOBAL acquisition_description String Samples were taken using a rosette of Niskin bottles, fixed with freshly
titrated paraformaldehyde (pH 7.4\u20138.1, 0.1% final concentration), held in
the dark for 10 min, frozen in liquid nitrogen, and stored in a -80 deg C
freezer (CH0409 samples) or in liquid nitrogen (CH0510 samples) until
analysis. Preserved samples were analyzed by dual beam flow cytometry on a
modified Coulter-EPICS 753 flow cytometer (Binder et al. 1996). Samples were
chosen in random order, defrosted in a 30\u00b0C water bath (just long enough
to melt, ~5 min), and stained with the DNA-specific stain Hoechst 33342 (0.5
ug mL-1 final concentration) (Invitrogen, Carlsbad, California) for a minimum
of 20 min in the dark. Prior to analysis, polystyrene fluorescent beads (0.5
um and 1.0 um diameter Flow CheckVR ; Polysciences, Washington, Pennsylvania),
were added to each sample, and used to normalize cellular light scatter and
red (chlorophyll-derived) fluorescence. Samples were run at an infusion rate
of 10 uL min-1 for 10\u201350 min, depending on cell abundance within the
sample.

Mean red fluorescence and forward angle light scatter for each cell type in
each sample are linearized and normalized to 1.0 um and 0.5 um diameter beads
(see above), respectively. Thus the measurements are relative, and are only
meaningful for comparisons of cellular properties within this data set.
attribute NC_GLOBAL awards_0_award_nid String 709338
attribute NC_GLOBAL awards_0_award_number String OCE-0751672
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0751672 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Dr David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Picoplankton Data
B. Binder, PI
Version 12 October 2017
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.2d 13 Jun 2019
attribute NC_GLOBAL date_created String 2017-10-12T21:08:22Z
attribute NC_GLOBAL date_modified String 2019-06-11T17:44:12Z
attribute NC_GLOBAL defaultDataQuery String &time
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.716814.1
attribute NC_GLOBAL Easternmost_Easting double -71.998
attribute NC_GLOBAL geospatial_lat_max double 30.874
attribute NC_GLOBAL geospatial_lat_min double 30.158
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -71.998
attribute NC_GLOBAL geospatial_lon_min double -72.756
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 125.0
attribute NC_GLOBAL geospatial_vertical_min double 40.0
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/716814 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Niskin bottle
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to take samples in rosette
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 716915
attribute NC_GLOBAL instruments_0_description String A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24 or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Niskin bottle
attribute NC_GLOBAL instruments_0_instrument_nid String 413
attribute NC_GLOBAL instruments_0_supplied_name String Niskin bottle
attribute NC_GLOBAL instruments_1_acronym String Flow Cytometer
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used to analyze preserved samples
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 716916
attribute NC_GLOBAL instruments_1_description String Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB37/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Flow Cytometer
attribute NC_GLOBAL instruments_1_instrument_nid String 660
attribute NC_GLOBAL instruments_1_supplied_name String Coulter-EPICS 753 flow cytometer
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, chemical, cruise, data, dataset, date, date_UTC, depth, dmo, erddap, fals, iso, latitude, longitude, management, oceanography, office, preliminary, pro, pro_fals, pro_red, red, syn, syn_fals, syn_red, time, time_UTC
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/716814 (external link)
attribute NC_GLOBAL Northernmost_Northing double 30.874
attribute NC_GLOBAL param_mapping String {'716814': {'Lat': 'master - latitude', 'Depth': 'flag - depth', 'Lon': 'master - longitude', 'ISO_DateTime_UTC': 'master - time'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/716814/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Georgia
attribute NC_GLOBAL people_0_affiliation_acronym String UGA
attribute NC_GLOBAL people_0_person_name String Dr Brian Binder
attribute NC_GLOBAL people_0_person_nid String 50893
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Hannah Ake
attribute NC_GLOBAL people_1_person_nid String 650173
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Application of a Reciprocal Transplant / Dilution Approach
attribute NC_GLOBAL projects_0_acronym String Picophytoplankton_Regulation
attribute NC_GLOBAL projects_0_description String The intellectual merit of the research is to extend our understanding of the biology and ecology of marine picophytoplankton, a group of microbes that are responsible for a large proportion of the total photosynthetic carbon fixation that occurs in the world's oceans. The importance of picophytoplankton as the dominant primary producers in open-ocean ecosystems is well-established. However, the factors that regulate the distribution and abundance of these populations remain poorly understood. The investigators will explore the dynamics of top-down (grazer-mediated) regulation of picophytoplankton populations in a specific context: the maintenance of summertime subsurface maxima in the pico-cyanobacterium Prochlorococcus (but not Synechococcus) in the Sargasso Sea. This phenomenon represents a relatively simple and predictable model system within which to test hypotheses about the regulation of oceanic picophytoplankton in general.
Recent results suggest that despite their abundance, Prochlorococcus in the subsurface maxi-mum are growing (and being grazed) rather slowly, as compared to the smaller population at the surface. In order to understand the factors responsible for this apparent paradox, this project will use a combination of field and laboratory studies to characterize and compare the interactions between Prochorococcus and its protozoan grazers at these two contrasting depths, and in relation to Synechococcus, which forms no such sub-surface maximum.
The broader impacts include training for graduate and undergraduate students. In addition, given the significance of picophytoplankton as primary producers at the base of oceanic microbial food webs, the results of this project should inform efforts to describe and model the broader oceanic ecosystem, and ultimately to understand its role in the global carbon cycle.
attribute NC_GLOBAL projects_0_end_date String 2013-02
attribute NC_GLOBAL projects_0_geolocation String Western Sargasso Sea (vicinity of 30 N 72 W)
attribute NC_GLOBAL projects_0_name String Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Application of a Reciprocal Transplant / Dilution Approach
attribute NC_GLOBAL projects_0_project_nid String 709339
attribute NC_GLOBAL projects_0_start_date String 2008-03
attribute NC_GLOBAL publisher_name String Hannah Ake
attribute NC_GLOBAL publisher_role String BCO-DMO Data Manager(s)
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 30.158
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v29
attribute NC_GLOBAL summary String Diel series of pico-cyanobacteria concentration and cell properties from the RV Cape Hatteras cruises CH0409 and CH0510 in the Western Sargasso Sea in 2009 and 2010.
attribute NC_GLOBAL time_coverage_end String 2010-05-31T13:29:00Z
attribute NC_GLOBAL time_coverage_start String 2009-05-26T17:58:00Z
attribute NC_GLOBAL title String Diel series of pico-cyanobacteria concentration and cell properties from the RV Cape Hatteras cruises CH0409 and CH0510 in the Western Sargasso Sea in 2009 and 2010.
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -72.756
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5-beta
variable Cruise   String  
attribute Cruise description String R/V Cape Hatteras Cruise Designation
attribute Cruise ioos_category String Unknown
attribute Cruise long_name String Cruise
attribute Cruise units String unitless
variable Wx   String  
attribute Wx description String Experiment Designation
attribute Wx ioos_category String Unknown
attribute Wx long_name String WX
attribute Wx units String unitless
variable date_UTC   String  
attribute date_UTC description String Sampling date; yyyy/mm/dd
attribute date_UTC ioos_category String Time
attribute date_UTC long_name String Date UTC
attribute date_UTC units String unitless
variable time_UTC   String  
attribute time_UTC description String Sampling time; hh:mm
attribute time_UTC ioos_category String Time
attribute time_UTC long_name String Time UTC
attribute time_UTC units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 30.158, 30.874
attribute latitude axis String Y
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Sampling latitude; E is positive
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -72.756, -71.998
attribute longitude axis String X
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Sampling longitude; N is positive
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable depth   double  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth _FillValue double NaN
attribute depth actual_range double 40.0, 125.0
attribute depth axis String Z
attribute depth colorBarMaximum double 8000.0
attribute depth colorBarMinimum double -8000.0
attribute depth colorBarPalette String TopographyDepth
attribute depth description String Sample depth
attribute depth ioos_category String Location
attribute depth long_name String Depth
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable pro   float  
attribute pro _FillValue float NaN
attribute pro actual_range float 209.0, 156000.0
attribute pro description String Prochlorococcus cellular concentration
attribute pro ioos_category String Unknown
attribute pro long_name String Pro
attribute pro units String cells per milliliter
variable syn   float  
attribute syn _FillValue float NaN
attribute syn actual_range float 0.0, 23300.0
attribute syn description String Synechococcus cellular concentration
attribute syn ioos_category String Unknown
attribute syn long_name String SYN
attribute syn units String cells per milliliter
variable pro_red   float  
attribute pro_red _FillValue float NaN
attribute pro_red actual_range float 0.0173, 1.57
attribute pro_red description String Mean Prochlorococcus cellular red fluorescence
attribute pro_red ioos_category String Unknown
attribute pro_red long_name String Pro Red
attribute pro_red units String relative
variable pro_fals   float  
attribute pro_fals _FillValue float NaN
attribute pro_fals actual_range float 0.0643, 0.823
attribute pro_fals description String Mean Prochlorococcus cellular forward angle light scatter
attribute pro_fals ioos_category String Unknown
attribute pro_fals long_name String Pro Fals
attribute pro_fals units String relative
variable syn_red   float  
attribute syn_red _FillValue float NaN
attribute syn_red actual_range float -0.00338, 2.02
attribute syn_red description String Mean Synechococcus cellular red fluorescence
attribute syn_red ioos_category String Unknown
attribute syn_red long_name String Syn Red
attribute syn_red units String relative
variable syn_fals   float  
attribute syn_fals _FillValue float NaN
attribute syn_fals actual_range float -3.69E-4, 2.54
attribute syn_fals description String Mean Synechococcus cellular forward angle light scatter
attribute syn_fals ioos_category String Unknown
attribute syn_fals long_name String Syn Fals
attribute syn_fals units String relative
variable time   double  
attribute time _CoordinateAxisType String Time
attribute time actual_range double 1.24336068E9, 1.27531254E9
attribute time axis String T
attribute time description String DateTime UTC; ISO formatted
attribute time ioos_category String Time
attribute time long_name String ISO Date Time UTC
attribute time source_name String ISO_DateTime_UTC
attribute time standard_name String time
attribute time time_origin String 01-JAN-1970 00:00:00
attribute time units String seconds since 1970-01-01T00:00:00Z

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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