ERDDAP > info > bcodmo_dataset_716979

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Cell counts in dilution experiment] - Prochlorococcus and Synechococcus cell counts in dilution experiment treatments from samples collected on RV Cape Hatteras cruises CH0409 and CH0510 in 2009 and 2010. (Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Application of a Reciprocal Transplant / Dilution Approach)		I   M	background			BCO-DMO	bcodmo_dataset_716979

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Dilution Experiments: For an overview of the purpose and interpretation of dilution experiments see Landry (1993) and Worden & Binder (2003). Seawater samples were taken with Go-Flo bottles suspended on non-metallic cable. A portion of this water was gravity-filtered through 0.2 um pore-size capsule filters (Whatman Polycap 36 TC), and appropriate volumes of filtered and unfiltered seawater were added to 500 ml polycarbonate bottles to achieve the indicated dilutions. Time-0 (T0) samples were removed from each bottle and preserved for later flow cytometric analysis as described below. Incubation bottles were then distributed to nylon mesh bags and resuspended in the water column at the depths indicated. After 24 hours, the bottles were recovered and sampled for time-final (TF) counts. All sampling gear, filters, and incubation bottles were acid-washed per Fitzwater et al. (1982). The dates, times, and locations of the experiments were as follows: \u00a0 Cruise\u00a0 \u00a0 \u00a0 Exper\u00a0 \u00a0 Date (UTC)\u00a0 \u00a0 T0 (UTC)\u00a0 \u00a0 \u00a0E Long\u00a0 \u00a0 \u00a0N Lat CH0409\u00a0 \u00a0 X1\u00a0 \u00a0 \u00a0 \u00a0 \u00a026-May-09\u00a0 \u00a0 \u00a017:58:30\u00a0 \u00a0 \u00a0-71.998\u00a0 \u00a0 30.171 CH0409\u00a0 \u00a0 X2\u00a0 \u00a0 \u00a0 \u00a0 \u00a029-May-09\u00a0 \u00a0 \u00a013:28:09\u00a0 \u00a0 \u00a0-72.002\u00a0 \u00a0 30.173 CH0510\u00a0 \u00a0 X2\u00a0 \u00a0 \u00a0 \u00a0 \u00a027-May-10\u00a0 \u00a0 \u00a013:33:30\u00a0 \u00a0 \u00a0-72.683\u00a0 \u00a0 30.699 \u00a0 Flow Cytometric Cell Counts: Samples were fixed with freshly titrated paraformaldehyde (pH 7.4\u20138.1, 0.1% final concentration), held in the dark for 10 min, frozen in liquid nitrogen, and stored in a \u201180 deg C freezer (CH0409 samples) or in liquid nitrogen (CH0510 samples) until analysis. Preserved samples were analyzed by flow cytometry on a modified Coulter-EPICS 753 flow cytometer (Binder et al. 1996). Samples were chosen in random order and defrosted in a 30 deg C water bath (just long enough to melt, ~5 min). Prior to analysis, polystyrene fluorescent beads (Flow Check\u00ae 0.494 um \u201cBB\u201d; Polysicences Inc., Washington, PA, USA), were added to each sample, and used to normalize cellular light scatter and fluorescence. Samples were typically run at an infusion rate of 20 uL min-1 for 1 to 20 min, depending on cell abundance within the sample. A minimum of 1,000 Prochlorococcus cells were analyzed, except for samples in which low cell concentrations made this impractical. Final cell concentrations tabulated here were calculated from 1-7 replicate counts (Count.n).
attribute	NC_GLOBAL	awards_0_award_nid	String	709338
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-0751672
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0751672
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Dilution Experiment - Cell Counts B. Binder, PI Version 12 October 2017
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2017-10-13T21:52:11Z
attribute	NC_GLOBAL	date_modified	String	2019-03-19T15:36:41Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.716979.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/716979
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	GO-FLO
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used to take seawater samples
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	717000
attribute	NC_GLOBAL	instruments_0_description	String	GO-FLO bottle cast used to collect water samples for pigment, nutrient, plankton, etc. The GO-FLO sampling bottle is specially designed to avoid sample contamination at the surface, internal spring contamination, loss of sample on deck (internal seals), and exchange of water from different depths.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/30/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	GO-FLO Bottle
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	411
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Go-flo bottle
attribute	NC_GLOBAL	instruments_1_acronym	String	Flow Cytometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Used to analyze preserved samples
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	716987
attribute	NC_GLOBAL	instruments_1_description	String	Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB37/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Flow Cytometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	660
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Coulter-EPICS 753 flow cytometer
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, bottle, chemical, count, cruise, data, dataset, depth, Depth_Incubate, Depth_Sample, dilution, dmo, erddap, exper, management, oceanography, office, preliminary, pro, syn, time, time2
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/716979/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/716979
attribute	NC_GLOBAL	param_mapping	String	{'716979': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/716979/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of Georgia
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UGA
attribute	NC_GLOBAL	people_0_person_name	String	Dr Brian Binder
attribute	NC_GLOBAL	people_0_person_nid	String	50893
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Hannah Ake
attribute	NC_GLOBAL	people_1_person_nid	String	650173
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	Picophytoplankton_Regulation
attribute	NC_GLOBAL	projects_0_acronym	String	Picophytoplankton_Regulation
attribute	NC_GLOBAL	projects_0_description	String	The intellectual merit of the research is to extend our understanding of the biology and ecology of marine picophytoplankton, a group of microbes that are responsible for a large proportion of the total photosynthetic carbon fixation that occurs in the world's oceans. The importance of picophytoplankton as the dominant primary producers in open-ocean ecosystems is well-established. However, the factors that regulate the distribution and abundance of these populations remain poorly understood. The investigators will explore the dynamics of top-down (grazer-mediated) regulation of picophytoplankton populations in a specific context: the maintenance of summertime subsurface maxima in the pico-cyanobacterium Prochlorococcus (but not Synechococcus) in the Sargasso Sea. This phenomenon represents a relatively simple and predictable model system within which to test hypotheses about the regulation of oceanic picophytoplankton in general. Recent results suggest that despite their abundance, Prochlorococcus in the subsurface maxi-mum are growing (and being grazed) rather slowly, as compared to the smaller population at the surface. In order to understand the factors responsible for this apparent paradox, this project will use a combination of field and laboratory studies to characterize and compare the interactions between Prochorococcus and its protozoan grazers at these two contrasting depths, and in relation to Synechococcus, which forms no such sub-surface maximum. The broader impacts include training for graduate and undergraduate students. In addition, given the significance of picophytoplankton as primary producers at the base of oceanic microbial food webs, the results of this project should inform efforts to describe and model the broader oceanic ecosystem, and ultimately to understand its role in the global carbon cycle.
attribute	NC_GLOBAL	projects_0_end_date	String	2013-02
attribute	NC_GLOBAL	projects_0_geolocation	String	Western Sargasso Sea (vicinity of 30 N 72 W)
attribute	NC_GLOBAL	projects_0_name	String	Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Application of a Reciprocal Transplant / Dilution Approach
attribute	NC_GLOBAL	projects_0_project_nid	String	709339
attribute	NC_GLOBAL	projects_0_start_date	String	2008-03
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Prochlorococcus and Synechococcus cell counts in dilution experiment treatments from samples collected on RV Cape Hatteras cruises CH0409 and CH0510 in 2009 and 2010.
attribute	NC_GLOBAL	title	String	[Cell counts in dilution experiment] - Prochlorococcus and Synechococcus cell counts in dilution experiment treatments from samples collected on RV Cape Hatteras cruises CH0409 and CH0510 in 2009 and 2010. (Top-Down Regulation of Picophytoplankton in the Sargasso Sea: Application of a Reciprocal Transplant / Dilution Approach)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Cruise		String
attribute	Cruise	bcodmo_name	String	cruise_id
attribute	Cruise	description	String	R/V Cape Hatteras Cruise Designation
attribute	Cruise	long_name	String	Cruise
attribute	Cruise	units	String	unitless
variable	Exper		String
attribute	Exper	bcodmo_name	String	exp_id
attribute	Exper	description	String	Experiment Designation
attribute	Exper	long_name	String	Exper
attribute	Exper	units	String	unitless
variable	Bottle		String
attribute	Bottle	bcodmo_name	String	bottle
attribute	Bottle	description	String	Unique incubation bottle identifier
attribute	Bottle	long_name	String	Bottle
attribute	Bottle	units	String	unitless
variable	Depth_Sample		byte
attribute	Depth_Sample	_FillValue	byte	127
attribute	Depth_Sample	actual_range	byte	20, 86
attribute	Depth_Sample	bcodmo_name	String	depth_w
attribute	Depth_Sample	colorBarMaximum	double	8000.0
attribute	Depth_Sample	colorBarMinimum	double	-8000.0
attribute	Depth_Sample	colorBarPalette	String	TopographyDepth
attribute	Depth_Sample	description	String	Depth of source water
attribute	Depth_Sample	long_name	String	Depth
attribute	Depth_Sample	standard_name	String	depth
attribute	Depth_Sample	units	String	meters
variable	Depth_Incubate		byte
attribute	Depth_Incubate	_FillValue	byte	127
attribute	Depth_Incubate	actual_range	byte	20, 86
attribute	Depth_Incubate	bcodmo_name	String	depth_in
attribute	Depth_Incubate	colorBarMaximum	double	8000.0
attribute	Depth_Incubate	colorBarMinimum	double	-8000.0
attribute	Depth_Incubate	colorBarPalette	String	TopographyDepth
attribute	Depth_Incubate	description	String	Depth of incubation
attribute	Depth_Incubate	long_name	String	Depth
attribute	Depth_Incubate	standard_name	String	depth
attribute	Depth_Incubate	units	String	meters
variable	Dilution		float
attribute	Dilution	_FillValue	float	NaN
attribute	Dilution	actual_range	float	0.1, 1.0
attribute	Dilution	bcodmo_name	String	dilution
attribute	Dilution	description	String	Fraction of unfiltered seawater in bottle
attribute	Dilution	long_name	String	Dilution
attribute	Dilution	units	String	number
variable	time2		String
attribute	time2	bcodmo_name	String	unknown
attribute	time2	description	String	Sampling time; timepoints
attribute	time2	long_name	String	Time
attribute	time2	units	String	unitless
variable	Pro		float
attribute	Pro	_FillValue	float	NaN
attribute	Pro	actual_range	float	826.0, 140000.0
attribute	Pro	bcodmo_name	String	cell_concentration
attribute	Pro	description	String	Prochlorococcus Cell Concentration
attribute	Pro	long_name	String	Pro
attribute	Pro	units	String	cells per milliliter
variable	Syn		float
attribute	Syn	_FillValue	float	NaN
attribute	Syn	actual_range	float	527.0, 35500.0
attribute	Syn	bcodmo_name	String	cell_concentration
attribute	Syn	description	String	Synechococcus Cell Concentration
attribute	Syn	long_name	String	SYN
attribute	Syn	units	String	cells per milliliter
variable	Count		byte
attribute	Count	_FillValue	byte	127
attribute	Count	actual_range	byte	1, 7
attribute	Count	bcodmo_name	String	count
attribute	Count	colorBarMaximum	double	100.0
attribute	Count	colorBarMinimum	double	0.0
attribute	Count	description	String	Number of counts underlying cell concentrations
attribute	Count	long_name	String	Count
attribute	Count	units	String	number

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.