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Title Sum-
Institution Dataset ID
   set  data   graph     files  public Bacterial protein production on particles obtained by gravity filtration of water collected
on RV/Endeavor EN556 (Patterns of activities project)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Seawater was transferred to 20 L carboys that were rinsed three times with
water from the sampling depth. Experiments on (operationally defined)
particles were carried out by gravity-filtering water through 3 um pore size
filters.\u00a0Fractions of the filters (1/8 of each filter) was incubated in
autoclaved seawater from the same depth/station; bacterial protein production
was calculated to account for the volume of seawater that had passed through
the filter.

Bacterial protein production was measured from 3H-leucine incorporation by
heterotrophic bacteria using the cold trichloroacetic acid (TCA) and
microcentrifuge extraction method [as in Kirchman, 2001]. All work was
performed aboard ship. In brief, triplicate live samples of 1.5 mL seawater as
well as one 100% (w/v) TCA-killed control were incubated with 23 uL of
L-[3,4,5-3H(N)]-Leucine (PerkinElmer, NET460250UC) for between 4 and 24 hours
in the dark at as close to in situ temperature as possible. Live samples were
then killed with 89 uL of 100% (w/v) TCA and centrifuged (10,000 rpm at
4\u00b0C for 10 min) to pelletize cell material.\u00a0 The supernatant liquid
was removed and 1 mL of 5% (w/v) TCA solution was added, followed by vortex
mixing and centrifugation. Supernatant removal, mixing, and centrifugation
were repeated using 1 mL of 80% ethanol solution.\u00a0 Finally, the
supernatant liquid was removed and each sample was dried overnight.\u00a0
After drying, 1 mL of scintillation cocktail (ScintiSafe 30% Cocktail, Fisher
SX23-5) was added and incorporated radioactivity was measured using an LSA
scintillation counter (PerkinElmer Tri-Carb 2910TR). Leucine incorporation
rate was calculated from the incorporated radioactivity, compared to 1 mL of
scintillation cocktail spiked with 23 uL of L-[3,4,5-3H(N)]-Leucine
radioactivity, divided by incubation time.
attribute NC_GLOBAL awards_0_award_nid String 712358
attribute NC_GLOBAL awards_0_award_number String OCE-1332881
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1332881 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Henrietta N Edmonds
attribute NC_GLOBAL awards_0_program_manager_nid String 51517
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Bacterial protein production from gravity filtering
EN556, Apr/May 2015
C. Arnosti (UNC)
version: 2017-10-27
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL dataset_current_state String Final and no updates
attribute NC_GLOBAL date_created String 2017-10-24T12:56:40Z
attribute NC_GLOBAL date_modified String 2020-05-13T15:54:06Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.717503.1
attribute NC_GLOBAL Easternmost_Easting double -68.4037
attribute NC_GLOBAL geospatial_lat_max double 40.0702
attribute NC_GLOBAL geospatial_lat_min double 37.6041
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -68.4037
attribute NC_GLOBAL geospatial_lon_min double -71.0052
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 4574.0
attribute NC_GLOBAL geospatial_vertical_min double 1.0
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/717503 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Niskin bottle
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to collect water for large volume mesocosm experiments
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 717509
attribute NC_GLOBAL instruments_0_description String A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Niskin bottle
attribute NC_GLOBAL instruments_0_instrument_nid String 413
attribute NC_GLOBAL instruments_0_supplied_name String 20 liter Niskin bottles
attribute NC_GLOBAL instruments_1_acronym String LSC
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used to measure incorporated radioactive 3H-leucine
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 717513
attribute NC_GLOBAL instruments_1_description String Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used the quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB21/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Liquid Scintillation Counter
attribute NC_GLOBAL instruments_1_instrument_nid String 624
attribute NC_GLOBAL instruments_1_supplied_name String LSA scintillation counter, PerkinElmer Tri-Carb 2910TR
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 717512
attribute NC_GLOBAL instruments_2_description String A device mounted on a ship that holds water samples under conditions of controlled temperature or controlled temperature and illumination.
attribute NC_GLOBAL instruments_2_instrument_name String Shipboard Incubator
attribute NC_GLOBAL instruments_2_instrument_nid String 629001
attribute NC_GLOBAL instruments_3_dataset_instrument_description String Used to concentrate cell material.
attribute NC_GLOBAL instruments_3_dataset_instrument_nid String 717511
attribute NC_GLOBAL instruments_3_description String A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids.
attribute NC_GLOBAL instruments_3_instrument_name String Centrifuge
attribute NC_GLOBAL instruments_3_instrument_nid String 629890
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, cast, chemical, cruise, cruise_id, data, dataset, density, depth, depth_id, depth_m, dmo, earth, Earth Science > Oceans > Salinity/Density > Salinity, elapsed, erddap, lat_degdecmin, latitude, leu, Leu_3H_rep_1, Leu_3H_rep_2, Leu_3H_rep_3, lon_degdecmin_W, longitude, management, ocean, oceanography, oceans, office, practical, preliminary, rep, salinity, science, sea, sea_water_practical_salinity, seawater, station, temperature, time, time_elapsed_hr, timepoint, water
attribute NC_GLOBAL keywords_vocabulary String GCMD Science Keywords
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/717503/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/717503 (external link)
attribute NC_GLOBAL Northernmost_Northing double 40.0702
attribute NC_GLOBAL param_mapping String {'717503': {'lat_dec': 'flag - latitude', 'depth_m': 'flag - depth', 'lon_dec': 'flag - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/717503/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of North Carolina at Chapel Hill
attribute NC_GLOBAL people_0_affiliation_acronym String UNC-Chapel Hill
attribute NC_GLOBAL people_0_person_name String Carol Arnosti
attribute NC_GLOBAL people_0_person_nid String 661940
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Nancy Copley
attribute NC_GLOBAL people_1_person_nid String 50396
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String Patterns of activities
attribute NC_GLOBAL projects_0_acronym String Patterns of activities
attribute NC_GLOBAL projects_0_description String NSF Award Abstract:
Heterotrophic microbial communities are key players in the marine carbon cycle, transforming and respiring organic carbon, regenerating nutrients, and acting as the final filter in sediments through which organic matter passes before long-term burial. Microbially-driven carbon cycling in the ocean profoundly affects the global carbon cycle, but key factors determining rates and locations of organic matter remineralization are unclear. In this study, researchers from the University of North Carolina at Chapel Hill will investigate the ability of pelagic microbial communities to initiate the remineralization of polysaccharides and proteins, which together constitute a major pool of organic matter in the ocean. Results from this study will be predictive on a large scale regarding the nature of the microbial response to organic matter input, and will provide a mechanistic framework for interpreting organic matter reactivity in the ocean.
Broader Impacts: This study will provide scientific training for undergraduate and graduate students from underrepresented groups. The project will also involve German colleagues, thus strengthening international scientific collaboration.
attribute NC_GLOBAL projects_0_end_date String 2017-07
attribute NC_GLOBAL projects_0_geolocation String Atlantic Ocean, Arctic Ocean, Pacific Ocean, Greenland
attribute NC_GLOBAL projects_0_name String Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?
attribute NC_GLOBAL projects_0_project_nid String 712359
attribute NC_GLOBAL projects_0_start_date String 2013-08
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 37.6041
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String cruise_id,timepoint,time_elapsed_hr
attribute NC_GLOBAL summary String Experiments on (operationally defined) particles were carried out by gravity-filtering water through 3 micron pore size filters. Bacterial protein production was measured from 3H-leucine incorporation by heterotrophic bacteria. See Niskin Bottle and Cast List EN556 to link specific casts and bottles to each experiment: https://www.bco-dmo.org/dataset/717427.
attribute NC_GLOBAL title String Bacterial protein production on particles obtained by gravity filtration of water collected on RV/Endeavor EN556 (Patterns of activities project)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -71.0052
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5
variable cruise_id   String  
attribute cruise_id bcodmo_name String cruise_id
attribute cruise_id description String cruise identifier
attribute cruise_id long_name String Cruise Id
attribute cruise_id units String unitless
variable cast   byte  
attribute cast _FillValue byte 127
attribute cast actual_range byte 4, 14
attribute cast bcodmo_name String cast
attribute cast description String cast number
attribute cast long_name String Cast
attribute cast units String unitless
variable station   byte  
attribute station _FillValue byte 127
attribute station actual_range byte 4, 8
attribute station bcodmo_name String station
attribute station description String station number
attribute station long_name String Station
attribute station units String unitless
variable depth_id   String  
attribute depth_id bcodmo_name String depth_comment
attribute depth_id description String depth description: sequence of depths sampled with 1 is surface and higher numbers at greater depths
attribute depth_id long_name String Depth
attribute depth_id standard_name String depth
attribute depth_id units String unitless
variable depth   double  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth _FillValue double NaN
attribute depth actual_range double 1.0, 4574.0
attribute depth axis String Z
attribute depth bcodmo_name String depth
attribute depth colorBarMaximum double 8000.0
attribute depth colorBarMinimum double -8000.0
attribute depth colorBarPalette String TopographyDepth
attribute depth description String actual depth at which water collected
attribute depth ioos_category String Location
attribute depth long_name String Depth
attribute depth nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable lat_degdecmin   String  
attribute lat_degdecmin bcodmo_name String latitude
attribute lat_degdecmin description String latitude formatted as degrees.decimal_minutes
attribute lat_degdecmin long_name String Latitude
attribute lat_degdecmin nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute lat_degdecmin standard_name String latitude
attribute lat_degdecmin units String degrees and decimal minutes
variable lon_degdecmin_W   String  
attribute lon_degdecmin_W bcodmo_name String longitude
attribute lon_degdecmin_W description String longitude formatted as degrees.decimal_minutes
attribute lon_degdecmin_W long_name String Longitude
attribute lon_degdecmin_W nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute lon_degdecmin_W standard_name String longitude
attribute lon_degdecmin_W units String degrees and decimal minutes
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 37.6041, 40.0702
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String latitude; north is positive
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude source_name String lat_dec
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -71.0052, -68.4037
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String longitude; north is positive
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude source_name String lon_dec
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable temp   float  
attribute temp _FillValue float NaN
attribute temp actual_range float 2.2, 18.7
attribute temp bcodmo_name String temperature
attribute temp description String water temperature as determined by CTD
attribute temp long_name String Temperature
attribute temp nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/ (external link)
attribute temp units String degrees Celsius
variable salinity   float  
attribute salinity _FillValue float NaN
attribute salinity actual_range float 34.879, 36.392
attribute salinity bcodmo_name String sal
attribute salinity colorBarMaximum double 37.0
attribute salinity colorBarMinimum double 32.0
attribute salinity description String salinity as determined by CTD
attribute salinity long_name String Sea Water Practical Salinity
attribute salinity nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PSALST01/ (external link)
attribute salinity units String per mil
variable timepoint   String  
attribute timepoint bcodmo_name String time_point
attribute timepoint description String sampling time point (0; 1; 2; etc.) post-incubation
attribute timepoint long_name String Timepoint
attribute timepoint units String unitless
variable time_elapsed_hr   byte  
attribute time_elapsed_hr _FillValue byte 127
attribute time_elapsed_hr actual_range byte 0, 0
attribute time_elapsed_hr bcodmo_name String time_elapsed
attribute time_elapsed_hr description String hours elapsed to reach a specific timepoint
attribute time_elapsed_hr long_name String Time Elapsed Hr
attribute time_elapsed_hr nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/ (external link)
attribute time_elapsed_hr units String hours
variable Leu_3H_rep_1   float  
attribute Leu_3H_rep_1 _FillValue float NaN
attribute Leu_3H_rep_1 actual_range float 0.002, 0.332
attribute Leu_3H_rep_1 bcodmo_name String leuc_incorp
attribute Leu_3H_rep_1 description String replicate 1 of leucine incorporation rate (bacterial protein production)
attribute Leu_3H_rep_1 long_name String Leu 3 H Rep 1
attribute Leu_3H_rep_1 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/UPLERIP4/ (external link)
attribute Leu_3H_rep_1 units String picomol leucine/liter/hour
variable Leu_3H_rep_2   float  
attribute Leu_3H_rep_2 _FillValue float NaN
attribute Leu_3H_rep_2 actual_range float 0.001, 0.305
attribute Leu_3H_rep_2 bcodmo_name String leuc_incorp
attribute Leu_3H_rep_2 description String replicate 2 of leucine incorporation rate (bacterial protein production)
attribute Leu_3H_rep_2 long_name String Leu 3 H Rep 2
attribute Leu_3H_rep_2 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/UPLERIP4/ (external link)
attribute Leu_3H_rep_2 units String picomol leucine/liter/hour
variable Leu_3H_rep_3   float  
attribute Leu_3H_rep_3 _FillValue float NaN
attribute Leu_3H_rep_3 actual_range float 0.001, 0.37
attribute Leu_3H_rep_3 bcodmo_name String leuc_incorp
attribute Leu_3H_rep_3 description String replicate 3 of leucine incorporation rate (bacterial protein production)
attribute Leu_3H_rep_3 long_name String Leu 3 H Rep 3
attribute Leu_3H_rep_3 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/UPLERIP4/ (external link)
attribute Leu_3H_rep_3 units String picomol leucine/liter/hour

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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