ERDDAP > info > bcodmo_dataset_717503

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
	set	data	graph		files	public	[EN556 gravity filtered particle-associated bacterial production] - Bacterial protein production on particles obtained by gravity filtration of water collected on RV/Endeavor EN556 (Patterns of activities project) (Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?)		F   I   M	background			BCO-DMO	bcodmo_dataset_717503

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute	NC_GLOBAL	acquisition_description	String	Seawater was transferred to 20 L carboys that were rinsed three times with water from the sampling depth. Experiments on (operationally defined) particles were carried out by gravity-filtering water through 3 um pore size filters.\u00a0Fractions of the filters (1/8 of each filter) was incubated in autoclaved seawater from the same depth/station; bacterial protein production was calculated to account for the volume of seawater that had passed through the filter. Bacterial protein production was measured from 3H-leucine incorporation by heterotrophic bacteria using the cold trichloroacetic acid (TCA) and microcentrifuge extraction method [as in Kirchman, 2001]. All work was performed aboard ship. In brief, triplicate live samples of 1.5 mL seawater as well as one 100% (w/v) TCA-killed control were incubated with 23 uL of L-[3,4,5-3H(N)]-Leucine (PerkinElmer, NET460250UC) for between 4 and 24 hours in the dark at as close to in situ temperature as possible. Live samples were then killed with 89 uL of 100% (w/v) TCA and centrifuged (10,000 rpm at 4\u00b0C for 10 min) to pelletize cell material.\u00a0 The supernatant liquid was removed and 1 mL of 5% (w/v) TCA solution was added, followed by vortex mixing and centrifugation. Supernatant removal, mixing, and centrifugation were repeated using 1 mL of 80% ethanol solution.\u00a0 Finally, the supernatant liquid was removed and each sample was dried overnight.\u00a0 After drying, 1 mL of scintillation cocktail (ScintiSafe 30% Cocktail, Fisher SX23-5) was added and incorporated radioactivity was measured using an LSA scintillation counter (PerkinElmer Tri-Carb 2910TR). Leucine incorporation rate was calculated from the incorporated radioactivity, compared to 1 mL of scintillation cocktail spiked with 23 uL of L-[3,4,5-3H(N)]-Leucine radioactivity, divided by incubation time.
attribute	NC_GLOBAL	awards_0_award_nid	String	712358
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1332881
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1332881
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Henrietta N Edmonds
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	51517
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Bacterial protein production from gravity filtering EN556, Apr/May 2015 C. Arnosti (UNC) version: 2017-10-27
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	dataset_current_state	String	Final and no updates
attribute	NC_GLOBAL	date_created	String	2017-10-24T12:56:40Z
attribute	NC_GLOBAL	date_modified	String	2020-05-13T15:54:06Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.717503.1
attribute	NC_GLOBAL	Easternmost_Easting	double	-68.4037
attribute	NC_GLOBAL	geospatial_lat_max	double	40.0702
attribute	NC_GLOBAL	geospatial_lat_min	double	37.6041
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	-68.4037
attribute	NC_GLOBAL	geospatial_lon_min	double	-71.0052
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	geospatial_vertical_max	double	4574.0
attribute	NC_GLOBAL	geospatial_vertical_min	double	1.0
attribute	NC_GLOBAL	geospatial_vertical_positive	String	down
attribute	NC_GLOBAL	geospatial_vertical_units	String	m
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/717503
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Niskin bottle
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used to collect water for large volume mesocosm experiments
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	717509
attribute	NC_GLOBAL	instruments_0_description	String	A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Niskin bottle
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	413
attribute	NC_GLOBAL	instruments_0_supplied_name	String	20 liter Niskin bottles
attribute	NC_GLOBAL	instruments_1_acronym	String	LSC
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Used to measure incorporated radioactive 3H-leucine
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	717513
attribute	NC_GLOBAL	instruments_1_description	String	Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used the quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB21/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Liquid Scintillation Counter
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	624
attribute	NC_GLOBAL	instruments_1_supplied_name	String	LSA scintillation counter, PerkinElmer Tri-Carb 2910TR
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	717512
attribute	NC_GLOBAL	instruments_2_description	String	A device mounted on a ship that holds water samples under conditions of controlled temperature or controlled temperature and illumination.
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Shipboard Incubator
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	629001
attribute	NC_GLOBAL	instruments_3_dataset_instrument_description	String	Used to concentrate cell material.
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	717511
attribute	NC_GLOBAL	instruments_3_description	String	A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids.
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Centrifuge
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	629890
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, cast, chemical, cruise, cruise_id, data, dataset, density, depth, depth_id, depth_m, dmo, earth, Earth Science > Oceans > Salinity/Density > Salinity, elapsed, erddap, lat_degdecmin, latitude, leu, Leu_3H_rep_1, Leu_3H_rep_2, Leu_3H_rep_3, lon_degdecmin_W, longitude, management, ocean, oceanography, oceans, office, practical, preliminary, rep, salinity, science, sea, sea_water_practical_salinity, seawater, station, temperature, time, time_elapsed_hr, timepoint, water
attribute	NC_GLOBAL	keywords_vocabulary	String	GCMD Science Keywords
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/717503/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/717503
attribute	NC_GLOBAL	Northernmost_Northing	double	40.0702
attribute	NC_GLOBAL	param_mapping	String	{'717503': {'lat_dec': 'flag - latitude', 'depth_m': 'flag - depth', 'lon_dec': 'flag - longitude'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/717503/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of North Carolina at Chapel Hill
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UNC-Chapel Hill
attribute	NC_GLOBAL	people_0_person_name	String	Carol Arnosti
attribute	NC_GLOBAL	people_0_person_nid	String	661940
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_1_person_nid	String	50396
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	Patterns of activities
attribute	NC_GLOBAL	projects_0_acronym	String	Patterns of activities
attribute	NC_GLOBAL	projects_0_description	String	NSF Award Abstract: Heterotrophic microbial communities are key players in the marine carbon cycle, transforming and respiring organic carbon, regenerating nutrients, and acting as the final filter in sediments through which organic matter passes before long-term burial. Microbially-driven carbon cycling in the ocean profoundly affects the global carbon cycle, but key factors determining rates and locations of organic matter remineralization are unclear. In this study, researchers from the University of North Carolina at Chapel Hill will investigate the ability of pelagic microbial communities to initiate the remineralization of polysaccharides and proteins, which together constitute a major pool of organic matter in the ocean. Results from this study will be predictive on a large scale regarding the nature of the microbial response to organic matter input, and will provide a mechanistic framework for interpreting organic matter reactivity in the ocean. Broader Impacts: This study will provide scientific training for undergraduate and graduate students from underrepresented groups. The project will also involve German colleagues, thus strengthening international scientific collaboration.
attribute	NC_GLOBAL	projects_0_end_date	String	2017-07
attribute	NC_GLOBAL	projects_0_geolocation	String	Atlantic Ocean, Arctic Ocean, Pacific Ocean, Greenland
attribute	NC_GLOBAL	projects_0_name	String	Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?
attribute	NC_GLOBAL	projects_0_project_nid	String	712359
attribute	NC_GLOBAL	projects_0_start_date	String	2013-08
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	37.6041
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	subsetVariables	String	cruise_id,timepoint,time_elapsed_hr
attribute	NC_GLOBAL	summary	String	Experiments on (operationally defined) particles were carried out by gravity-filtering water through 3 micron pore size filters. Bacterial protein production was measured from 3H-leucine incorporation by heterotrophic bacteria. See Niskin Bottle and Cast List EN556 to link specific casts and bottles to each experiment: https://www.bco-dmo.org/dataset/717427.
attribute	NC_GLOBAL	title	String	[EN556 gravity filtered particle-associated bacterial production] - Bacterial protein production on particles obtained by gravity filtration of water collected on RV/Endeavor EN556 (Patterns of activities project) (Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	Westernmost_Easting	double	-71.0052
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.5
variable	cruise_id		String
attribute	cruise_id	bcodmo_name	String	cruise_id
attribute	cruise_id	description	String	cruise identifier
attribute	cruise_id	long_name	String	Cruise Id
attribute	cruise_id	units	String	unitless
variable	cast		byte
attribute	cast	_FillValue	byte	127
attribute	cast	actual_range	byte	4, 14
attribute	cast	bcodmo_name	String	cast
attribute	cast	description	String	cast number
attribute	cast	long_name	String	Cast
attribute	cast	units	String	unitless
variable	station		byte
attribute	station	_FillValue	byte	127
attribute	station	actual_range	byte	4, 8
attribute	station	bcodmo_name	String	station
attribute	station	description	String	station number
attribute	station	long_name	String	Station
attribute	station	units	String	unitless
variable	depth_id		String
attribute	depth_id	bcodmo_name	String	depth_comment
attribute	depth_id	description	String	depth description: sequence of depths sampled with 1 is surface and higher numbers at greater depths
attribute	depth_id	long_name	String	Depth
attribute	depth_id	standard_name	String	depth
attribute	depth_id	units	String	unitless
variable	depth		double
attribute	depth	_CoordinateAxisType	String	Height
attribute	depth	_CoordinateZisPositive	String	down
attribute	depth	_FillValue	double	NaN
attribute	depth	actual_range	double	1.0, 4574.0
attribute	depth	axis	String	Z
attribute	depth	bcodmo_name	String	depth
attribute	depth	colorBarMaximum	double	8000.0
attribute	depth	colorBarMinimum	double	-8000.0
attribute	depth	colorBarPalette	String	TopographyDepth
attribute	depth	description	String	actual depth at which water collected
attribute	depth	ioos_category	String	Location
attribute	depth	long_name	String	Depth
attribute	depth	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/DEPH/
attribute	depth	positive	String	down
attribute	depth	standard_name	String	depth
attribute	depth	units	String	m
variable	lat_degdecmin		String
attribute	lat_degdecmin	bcodmo_name	String	latitude
attribute	lat_degdecmin	description	String	latitude formatted as degrees.decimal_minutes
attribute	lat_degdecmin	long_name	String	Latitude
attribute	lat_degdecmin	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LATX/
attribute	lat_degdecmin	standard_name	String	latitude
attribute	lat_degdecmin	units	String	degrees and decimal minutes
variable	lon_degdecmin_W		String
attribute	lon_degdecmin_W	bcodmo_name	String	longitude
attribute	lon_degdecmin_W	description	String	longitude formatted as degrees.decimal_minutes
attribute	lon_degdecmin_W	long_name	String	Longitude
attribute	lon_degdecmin_W	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LONX/
attribute	lon_degdecmin_W	standard_name	String	longitude
attribute	lon_degdecmin_W	units	String	degrees and decimal minutes
variable	latitude		double
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	_FillValue	double	NaN
attribute	latitude	actual_range	double	37.6041, 40.0702
attribute	latitude	axis	String	Y
attribute	latitude	bcodmo_name	String	latitude
attribute	latitude	colorBarMaximum	double	90.0
attribute	latitude	colorBarMinimum	double	-90.0
attribute	latitude	description	String	latitude; north is positive
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LATX/
attribute	latitude	source_name	String	lat_dec
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	longitude		double
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	_FillValue	double	NaN
attribute	longitude	actual_range	double	-71.0052, -68.4037
attribute	longitude	axis	String	X
attribute	longitude	bcodmo_name	String	longitude
attribute	longitude	colorBarMaximum	double	180.0
attribute	longitude	colorBarMinimum	double	-180.0
attribute	longitude	description	String	longitude; north is positive
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LONX/
attribute	longitude	source_name	String	lon_dec
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east
variable	temp		float
attribute	temp	_FillValue	float	NaN
attribute	temp	actual_range	float	2.2, 18.7
attribute	temp	bcodmo_name	String	temperature
attribute	temp	description	String	water temperature as determined by CTD
attribute	temp	long_name	String	Temperature
attribute	temp	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/
attribute	temp	units	String	degrees Celsius
variable	salinity		float
attribute	salinity	_FillValue	float	NaN
attribute	salinity	actual_range	float	34.879, 36.392
attribute	salinity	bcodmo_name	String	sal
attribute	salinity	colorBarMaximum	double	37.0
attribute	salinity	colorBarMinimum	double	32.0
attribute	salinity	description	String	salinity as determined by CTD
attribute	salinity	long_name	String	Sea Water Practical Salinity
attribute	salinity	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PSALST01/
attribute	salinity	units	String	per mil
variable	timepoint		String
attribute	timepoint	bcodmo_name	String	time_point
attribute	timepoint	description	String	sampling time point (0; 1; 2; etc.) post-incubation
attribute	timepoint	long_name	String	Timepoint
attribute	timepoint	units	String	unitless
variable	time_elapsed_hr		byte
attribute	time_elapsed_hr	_FillValue	byte	127
attribute	time_elapsed_hr	actual_range	byte	0, 0
attribute	time_elapsed_hr	bcodmo_name	String	time_elapsed
attribute	time_elapsed_hr	description	String	hours elapsed to reach a specific timepoint
attribute	time_elapsed_hr	long_name	String	Time Elapsed Hr
attribute	time_elapsed_hr	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/
attribute	time_elapsed_hr	units	String	hours
variable	Leu_3H_rep_1		float
attribute	Leu_3H_rep_1	_FillValue	float	NaN
attribute	Leu_3H_rep_1	actual_range	float	0.002, 0.332
attribute	Leu_3H_rep_1	bcodmo_name	String	leuc_incorp
attribute	Leu_3H_rep_1	description	String	replicate 1 of leucine incorporation rate (bacterial protein production)
attribute	Leu_3H_rep_1	long_name	String	Leu 3 H Rep 1
attribute	Leu_3H_rep_1	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/UPLERIP4/
attribute	Leu_3H_rep_1	units	String	picomol leucine/liter/hour
variable	Leu_3H_rep_2		float
attribute	Leu_3H_rep_2	_FillValue	float	NaN
attribute	Leu_3H_rep_2	actual_range	float	0.001, 0.305
attribute	Leu_3H_rep_2	bcodmo_name	String	leuc_incorp
attribute	Leu_3H_rep_2	description	String	replicate 2 of leucine incorporation rate (bacterial protein production)
attribute	Leu_3H_rep_2	long_name	String	Leu 3 H Rep 2
attribute	Leu_3H_rep_2	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/UPLERIP4/
attribute	Leu_3H_rep_2	units	String	picomol leucine/liter/hour
variable	Leu_3H_rep_3		float
attribute	Leu_3H_rep_3	_FillValue	float	NaN
attribute	Leu_3H_rep_3	actual_range	float	0.001, 0.37
attribute	Leu_3H_rep_3	bcodmo_name	String	leuc_incorp
attribute	Leu_3H_rep_3	description	String	replicate 3 of leucine incorporation rate (bacterial protein production)
attribute	Leu_3H_rep_3	long_name	String	Leu 3 H Rep 3
attribute	Leu_3H_rep_3	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/UPLERIP4/
attribute	Leu_3H_rep_3	units	String	picomol leucine/liter/hour

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.