ERDDAP > info > bcodmo_dataset_717660

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
	set	data	graph		files	public	[Greenland experiment: microbial enzymatic activities] - Microbial enzymatic activities from seawater and from particle-associated seawater communities from Greenland, August 2015 (Patterns of activities project) (Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?)		I   M	background			BCO-DMO	bcodmo_dataset_717660

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Using a small boat, samples were collected in 20L carboys in Tylerfjord-Young Sound. Three rivers that feed into Tyrolerfjord-Young Sound (Tyroler River, Lerbugten River and Zackenberg River) were sampled; surface and subsurface water samples were also collected at transition sites where the rivers feed into the fjord (Tyro_01, Zac_30, Ler_30, altogether referred to as \u2018river transition sites\u2019). Enzyme activities were measured in unfiltered water. In addition, water was size-fractionated using gravity filtration through a GF/A filter to capture \u22651.6 \u00b5m particles. Two substrates, a-glucose and b-glucose linked to a 4-methylumbelliferyl (MUF) fluorophore, were used to measure glucosidase activities. Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid \u2013 leucine \u2013 and four oligopeptides \u2013 the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and phenylalanine-serine-arginine (FSR) \u2013 were used to measure exo- and endo- acting peptidase activities, respectively. Hydrolysis rates of the substrates were measured as an increase in fluorescence as the fluorophore was hydrolyzed from the substrate over time [as in Hoppe, 1993; Obayashi and Suzuki, 2005]. \u00a0All substrates were used to measure enzyme activities in unfiltered water, as well as particle-associated (\u22651.6 \u00b5m) enzymatic activities. In unfiltered water, enzyme activities were measured by adding 4 mL of water to triplicate cuvettes. One incubation containing autoclaved water served as the killed control. This procedure was applied to each of the 7 substrates and one live blank and autoclave blank (no substrate addition). Each cuvette containing either live or autoclaved water was amended with one substrate to a concentration of 100 \u00b5M. Fluroescence was measured using a Promega Quanti\ufb02uor solid-state single-cuvette \ufb02uorimeter; excitation and emission maxima were 365 nm and 410\u2013450 nm, respectively, To measure particle-associated enzyme assays, 1/12th piece of a GF/A filter through which water had been gravity filtered was put into a cuvette containing 4 mL of cooled, autoclaved water from the same station/depth as the live samples. In addition, killed controls were set up using sterile GF/A filters cut into 1/12th pieces. Bulk water and particle-associated enzyme assays were incubated for up to 24 and 16 hours, respectively; timepoints were taken at specific intervals. Incubations were kept in the dark either at 0\u00b0C, 5\u00b0C, or 8\u00b0C, depending on in situ water temperature at the time of sampling. Activities of polysaccharide hydrolases were measured using fluorescently labeled polysaccharides (Arnosti 2003). Activities of enzymes that hydrolyze pullulan, laminarin, xylan, fucoidan, arabinogalactan, and chondroitin sulfate were measured in unfiltered water, and using GF/A filters through which water had been gravity-filtered. For these measurements, substrate was added (of 3.5 \u00b5M monomer equivalent) to 15 mL of water; autoclaved ambient water served as the killed control. Particle-associated activities were measured by submerging 1/12th of a GF/A filter in 15 ml autoclaved seawater. Samples were incubated in the dark at near in situ temperature (0\u00b0C, 5\u00b0C, or 8\u00b0C), and sub-sampled at specific time intervals\u2014t0 (0h, upon substrate addition), t1 (120 h), t2 (240 h), t3 (360 h) and t4 (600 h). Sub- samples from each timepoint were filtered using 0.2 \u00b5M pore size SFCA (surfactant-free cellulose acetate) syringe filters, and the filtrate was collected in tubes and frozen at -20\u00b0C until processing in the lab. Sub- samples were processed using gel permeation chromatography (Arnosti, 2003).\u00a0
attribute	NC_GLOBAL	awards_0_award_nid	String	712358
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1332881
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1332881
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Henrietta N Edmonds
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	51517
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Microbial enzyme activities and bacterial productivity: hydrolysis rates Greenland bulk (unfiltered) and GF filtered samples C. Arnosti (UNC) version: 2017-10-30
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	dataset_current_state	String	Final and no updates
attribute	NC_GLOBAL	date_created	String	2017-10-25T16:47:01Z
attribute	NC_GLOBAL	date_modified	String	2020-05-13T14:07:45Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.717660.1
attribute	NC_GLOBAL	geospatial_vertical_max	double	20.0
attribute	NC_GLOBAL	geospatial_vertical_min	double	1.0
attribute	NC_GLOBAL	geospatial_vertical_positive	String	down
attribute	NC_GLOBAL	geospatial_vertical_units	String	m
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/717660
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Niskin bottle
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used to collect water for large volume mesocosm experiments
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	717666
attribute	NC_GLOBAL	instruments_0_description	String	A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Niskin bottle
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	413
attribute	NC_GLOBAL	instruments_0_supplied_name	String	20 liter Niskin bottles
attribute	NC_GLOBAL	instruments_1_acronym	String	Fluorometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	718104
attribute	NC_GLOBAL	instruments_1_description	String	A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/113/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Fluorometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	484
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Promega Quantifluor solid-state single-cuvette fluorimeter
attribute	NC_GLOBAL	keywords	String	average, bco, bco-dmo, biological, cast, chemical, data, dataset, depth, depth_id, depth_m, dev, dmo, elapsed, erddap, filter, filter_um, fluorophore, management, oceanography, office, preliminary, profiler, rate, rate_average, rate_std_dev, rep1, rep1_rate, rep2, rep2_rate, rep3, rep3_rate, salinity, salinity-temperature-depth, sample, sample_type, station, std, substrate, temperature, time, time_elapsed_hr, timepoint, trip, trip_id, type
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/717660/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/717660
attribute	NC_GLOBAL	param_mapping	String	{'717660': {'depth_m': 'master - depth'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/717660/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of North Carolina at Chapel Hill
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UNC-Chapel Hill
attribute	NC_GLOBAL	people_0_person_name	String	Carol Arnosti
attribute	NC_GLOBAL	people_0_person_nid	String	661940
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_1_person_nid	String	50396
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	Patterns of activities
attribute	NC_GLOBAL	projects_0_acronym	String	Patterns of activities
attribute	NC_GLOBAL	projects_0_description	String	NSF Award Abstract: Heterotrophic microbial communities are key players in the marine carbon cycle, transforming and respiring organic carbon, regenerating nutrients, and acting as the final filter in sediments through which organic matter passes before long-term burial. Microbially-driven carbon cycling in the ocean profoundly affects the global carbon cycle, but key factors determining rates and locations of organic matter remineralization are unclear. In this study, researchers from the University of North Carolina at Chapel Hill will investigate the ability of pelagic microbial communities to initiate the remineralization of polysaccharides and proteins, which together constitute a major pool of organic matter in the ocean. Results from this study will be predictive on a large scale regarding the nature of the microbial response to organic matter input, and will provide a mechanistic framework for interpreting organic matter reactivity in the ocean. Broader Impacts: This study will provide scientific training for undergraduate and graduate students from underrepresented groups. The project will also involve German colleagues, thus strengthening international scientific collaboration.
attribute	NC_GLOBAL	projects_0_end_date	String	2017-07
attribute	NC_GLOBAL	projects_0_geolocation	String	Atlantic Ocean, Arctic Ocean, Pacific Ocean, Greenland
attribute	NC_GLOBAL	projects_0_name	String	Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?
attribute	NC_GLOBAL	projects_0_project_nid	String	712359
attribute	NC_GLOBAL	projects_0_start_date	String	2013-08
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	subsetVariables	String	trip_id
attribute	NC_GLOBAL	summary	String	Bacterial activity as measured by hydrolysis rates from unfiltered seawater and particle-associated communities collected near shore in northeastern Greenland in August 2015.
attribute	NC_GLOBAL	title	String	[Greenland experiment: microbial enzymatic activities] - Microbial enzymatic activities from seawater and from particle-associated seawater communities from Greenland, August 2015 (Patterns of activities project) (Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.5
variable	trip_id		String
attribute	trip_id	bcodmo_name	String	Cruise Name
attribute	trip_id	description	String	sampling trip identifier; YS means Young Sound
attribute	trip_id	long_name	String	Trip Id
attribute	trip_id	units	String	unitless
variable	sample_type		String
attribute	sample_type	bcodmo_name	String	sample_type
attribute	sample_type	description	String	indication of whether sample was filtered (GF) or not (bulk)
attribute	sample_type	long_name	String	Sample Type
attribute	sample_type	units	String	unitless
variable	filter_um		float
attribute	filter_um	_FillValue	float	NaN
attribute	filter_um	actual_range	float	1.6, 1.6
attribute	filter_um	bcodmo_name	String	filter_size
attribute	filter_um	description	String	filter size
attribute	filter_um	long_name	String	Filter Um
attribute	filter_um	units	String	nanomol monomer/liter/hour
variable	fluorophore		String
attribute	fluorophore	bcodmo_name	String	unknown
attribute	fluorophore	description	String	fluorescent molecules used to measure hydrolysis rates: fluorescently-labeled polysaccharides (FLA) or small substrate proxies tagged with methylcoumarine (MCA) and methylumbelliferone (MUF) fluorophores.
attribute	fluorophore	long_name	String	Fluorophore
attribute	fluorophore	units	String	unitless
variable	station		String
attribute	station	bcodmo_name	String	station
attribute	station	description	String	station identifier
attribute	station	long_name	String	Station
attribute	station	units	String	unitless
variable	cast		int
attribute	cast	_FillValue	int	2147483647
attribute	cast	actual_range	int	120808, 171521
attribute	cast	bcodmo_name	String	cast
attribute	cast	description	String	cast identifier
attribute	cast	long_name	String	Cast
attribute	cast	units	String	unitless
variable	depth_id		String
attribute	depth_id	bcodmo_name	String	depth_comment
attribute	depth_id	description	String	depth description: sequence of depths sampled with 1 is surface and higher numbers at greater depths
attribute	depth_id	long_name	String	Depth
attribute	depth_id	standard_name	String	depth
attribute	depth_id	units	String	unitless
variable	depth		double
attribute	depth	_CoordinateAxisType	String	Height
attribute	depth	_CoordinateZisPositive	String	down
attribute	depth	_FillValue	double	NaN
attribute	depth	actual_range	double	1.0, 20.0
attribute	depth	axis	String	Z
attribute	depth	bcodmo_name	String	depth
attribute	depth	colorBarMaximum	double	8000.0
attribute	depth	colorBarMinimum	double	-8000.0
attribute	depth	colorBarPalette	String	TopographyDepth
attribute	depth	description	String	actual depth at which water collected
attribute	depth	ioos_category	String	Location
attribute	depth	long_name	String	Depth
attribute	depth	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/DEPH/
attribute	depth	positive	String	down
attribute	depth	standard_name	String	depth
attribute	depth	units	String	m
variable	substrate		String
attribute	substrate	bcodmo_name	String	unknown
attribute	substrate	description	String	substrates for measurement of enzymatic activities: ara = arabinogalactan; chn = chondroitin sulfate; fuc = fucoidan; lam = laminarin; pul = pullulan; xyl = xylan
attribute	substrate	long_name	String	Substrate
attribute	substrate	units	String	unitless
variable	timepoint		String
attribute	timepoint	bcodmo_name	String	time_point
attribute	timepoint	description	String	sampling time point (0; 1; 2; etc.) post-incubation
attribute	timepoint	long_name	String	Timepoint
attribute	timepoint	units	String	unitless
variable	time_elapsed_hr		short
attribute	time_elapsed_hr	_FillValue	short	32767
attribute	time_elapsed_hr	actual_range	short	0, 982
attribute	time_elapsed_hr	bcodmo_name	String	time_elapsed
attribute	time_elapsed_hr	description	String	hours elapsed to reach a specific timepoint
attribute	time_elapsed_hr	long_name	String	Time Elapsed Hr
attribute	time_elapsed_hr	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/
attribute	time_elapsed_hr	units	String	hours
variable	rep1_rate		float
attribute	rep1_rate	_FillValue	float	NaN
attribute	rep1_rate	actual_range	float	0.0, 76.82
attribute	rep1_rate	bcodmo_name	String	unknown
attribute	rep1_rate	description	String	replicate 1 hydrolysis rate
attribute	rep1_rate	long_name	String	Rep1 Rate
attribute	rep1_rate	units	String	nanomol monomer/liter/hour
variable	rep2_rate		float
attribute	rep2_rate	_FillValue	float	NaN
attribute	rep2_rate	actual_range	float	0.0, 77.36
attribute	rep2_rate	bcodmo_name	String	unknown
attribute	rep2_rate	description	String	replicate 2 hydrolysis rate
attribute	rep2_rate	long_name	String	Rep2 Rate
attribute	rep2_rate	units	String	nanomol monomer/liter/hour
variable	rep3_rate		float
attribute	rep3_rate	_FillValue	float	NaN
attribute	rep3_rate	actual_range	float	0.0, 91.25
attribute	rep3_rate	bcodmo_name	String	unknown
attribute	rep3_rate	description	String	replicate 3 hydrolysis rate
attribute	rep3_rate	long_name	String	Rep3 Rate
attribute	rep3_rate	units	String	nanomol monomer/liter/hour
variable	rate_average		float
attribute	rate_average	_FillValue	float	NaN
attribute	rate_average	actual_range	float	0.0, 81.81
attribute	rate_average	bcodmo_name	String	unknown
attribute	rate_average	description	String	average of the 3 hydrolysis rates
attribute	rate_average	long_name	String	Rate Average
attribute	rate_average	units	String	nanomol monomer/liter/hour
variable	rate_std_dev		String
attribute	rate_std_dev	bcodmo_name	String	unknown
attribute	rate_std_dev	description	String	standard deviation of the 3 hydrolysis rates
attribute	rate_std_dev	long_name	String	Rate Std Dev
attribute	rate_std_dev	units	String	nanomol monomer/liter/hour

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.