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   set  data   graph     files  public Fatty acid profiles of M. menidia females and their unfertilized eggs.    ?     I   M   background (external link) RSS BCO-DMO bcodmo_dataset_719379

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Methodology from\u00a0Snyder, J.T.*, Murray, C.S.*, and Baumann, H. (2017)
Potential for maternal effects on offspring CO2-sensitivities in a coastal
marine fish. Journal of Experimental Marine Biology and Ecology (in press).

Five randomly selected females were strip-spawned onto cutout sections of
window screen (1-mm mesh) that were placed into separate seawater-filled
spawning dishes (Murray et al., 2014). To ensure full fertilization success
and randomize potential paternal effects, eggs were fertilized with a mixture
of milt from 22 males, thus producing full-sib and maternal half-sib embryos
from each female. Adults were measured for total length (TL; mean TLmale =
9.14 cm, mean TLfemale = 10.4 cm) and frozen for later analysis of FA. Mesh
screens with attached embryos were subsequently cut into smaller sections to
allow precise enumeration, and within 2-hr post-fertilization 100 embryos were
placed into each of three replicate rearing containers (20 L) per female and
CO2 treatment (i.e., 600 embryos for each of five females, 3 \u00d7 100 in
ambient and 3 \u00d7 100 in acidified treatments). Rearing containers were
filled with 1-um filtered, UV-sterilized seawater (~30 psu) from Long Island
Sound and placed in temperature-controlled water baths set to 24 deg C, the
known thermal optimum for survival and growth in this species (Middaugh et
al., 1987). Offspring were reared for 24 d post fertilization under a 15h
light:9h dark lighting regime. After hatch, larvae were fed ad libitum rations
of newly hatched brine shrimp nauplii Artemia salina (brineshrimpdirect.com),
and 50% of water was replaced every 5 d to ensure safe ammonia levels (< 0.25
ppm). Hatched larvae were counted and subsampled (n = 10 per replicate) at 1 d
post hatch (dph) by gently scooping them into identical 20 L containers, and
final samples were taken at 16 dph. All samples were preserved in 5% buffered
formalin for later measurements of larval standard length (SL, 0.01 mm) via
calibrated digital images (ImagePro Premier, MediaCybernetics). The experiment
thus quantified three related survival and three size traits for each
replicate, female, and CO2 treatment: embryo survival (fertilization to 1
dph), larval survival (1 to 16 dph), overall survival (fertilization to 16
dph), size (SL) at hatch (1 dph), SL at 16 dph, and larval growth rate (GR =
(SL16dph \u2013 SL1dph)/15).

CO2 regime:\u00a0

Offspring were reared at ambient (~ 400 uatm, pHNBS = 8.18) and acidified CO2
conditions (~2,300 uatm, pHNBS = 7.50). The higher value was set to a level
commonly used in OA research (consistent with projections of future pCO2
values for open oceans over in the next 200 yr (IPCC, 2007)) and represents
current conditions experienced during seasonal extremes by this species in
nature (Murray et al., 2014). Ambient conditions were achieved by bubbling
partially CO2-stripped air into each rearing container, thereby offsetting
metabolic CO2 accumulation. Acidified conditions were achieved via gas
proportioners (Cole Parmer\u00ae) that mixed CO2 stripped air with 100% bone-
dry CO2 delivered to the bottom of each rearing container via air stones.
Target pH and temperature were monitored daily via a handheld pH probe
(Hach\u00ae HQ40d portable meter with a PHC201 standard pH-probe) calibrated
regularly via two-point National Bureau of Standards (NBS) pH buffers
(electronic supplementary material, Fig.S1). To characterize actual pCO2
levels and related water chemistry parameters, water was sampled from four
randomly chosen rearing containers per treatment three times over the course
of the experiment and immediately measured for total alkalinity (AT) via
endpoint titration (Mettler Toledo\u2122 G20 Potentiometric Titrator). The
instrument has previously been shown to quantify AT in Dr. Andrew
Dickson\u2019s reference material (batch 147, AT= 2231.39 umol kg seawater-1)
with an average error of 0.6%. Actual levels of total dissolved inorganic
carbon (CT), partial pressure of CO2 (pCO2), fugacity of CO2 (fCO2), and
carbonate ion concentration were calculated in CO2SYS
based on measured AT, pH (NBS), temperature, and salinity using K1 and K2
constants from Mehrbach et al. (1973) refit by Dickson and Millero (1987) and
Dickson (1990) for KHSO4 (Table 1).

Fatty acid analysis:\u00a0

Gas chromatography was used to quantify the absolute (mg g dry weight-1) and
relative concentrations (% of total) of 27 FAs for each of the 5 females
(whole individual) and their unfertilized eggs (~ 1 ml) following the methods
of Faulk and Holt (2005) as recently used for this species in Murray et al.
(2016). Briefly, frozen samples were shipped on dry ice to the Fisheries and
Mariculture Laboratory (University of Texas, Marine Science Institute), where
they were first dried and then homogenized in a solution of chloroform-
methanol (2:1 v/v) and tricosanoic acid (23:0) as an internal standard for
quantification of mg g\u22121 dry mass of fatty acids. Lipids were cold-
extracted from approximately 50 mg of dry mass. Fatty-acid methyl esters were
prepared by saponification in potassium hydroxide, followed by
transesterification with 14% boron trifluoride in methanol. A Shimadzu GC-2014
gas chromatograph set with a Phenomenex ZB-WAX plus capillary column (30 m
long; 0.53 mm ID; 1.0 um thick) was used to quantify FAs, and individual FAs
were identified by comparison to commercial standards (Supelco, Inc). Two FAs,
12:0 and 15:1, were below detection limit or invariant across egg batches and
were therefore excluded from subsequent analyses.
attribute NC_GLOBAL awards_0_award_nid String 650183
attribute NC_GLOBAL awards_0_award_number String OCE-1536336
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1536336 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Dr Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Fatty Acid Profiles
H. Baumann and J. Nye, PIs
Version November 14th 2017
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.2d 13 Jun 2019
attribute NC_GLOBAL date_created String 2017-11-15T18:26:06Z
attribute NC_GLOBAL date_modified String 2017-11-15T22:42:11Z
attribute NC_GLOBAL defaultDataQuery String &time
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.719454
attribute NC_GLOBAL Easternmost_Easting double -72.02
attribute NC_GLOBAL geospatial_lat_max double 41.32
attribute NC_GLOBAL geospatial_lat_min double 41.32
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -72.02
attribute NC_GLOBAL geospatial_lon_min double -72.02
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/719379 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Gas Chromatograph
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to quantify fatty acids
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 719394
attribute NC_GLOBAL instruments_0_description String Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB02/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Gas Chromatograph
attribute NC_GLOBAL instruments_0_instrument_nid String 661
attribute NC_GLOBAL instruments_0_supplied_name String Shimadzu GC-2014 gas chromatograph set with a Phenomenex ZB-WAX plus capillary column
attribute NC_GLOBAL instruments_1_acronym String pH Sensor
attribute NC_GLOBAL instruments_1_dataset_instrument_description String handheld pH probe
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 719388
attribute NC_GLOBAL instruments_1_description String General term for an instrument that measures the pH or how acidic or basic a solution is.
attribute NC_GLOBAL instruments_1_instrument_name String pH Sensor
attribute NC_GLOBAL instruments_1_instrument_nid String 674
attribute NC_GLOBAL instruments_1_supplied_name String Hach� HQ40d portable meter with a PHC201 standard pH-probe
attribute NC_GLOBAL instruments_2_acronym String Automatic titrator
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Used to measure total alkalinity
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 719393
attribute NC_GLOBAL instruments_2_description String Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB12/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Automatic titrator
attribute NC_GLOBAL instruments_2_instrument_nid String 682
attribute NC_GLOBAL instruments_2_supplied_name String Mettler Toledo™�G20 Potentiometric Titrator
attribute NC_GLOBAL keywords String absolute, Absolute_concentration_eggs, Absolute_concentration_spawning_adult, acid, adult, bco, bco-dmo, biological, biology, chemical, collection, Collection_site, concentration, data, dataset, date, dmo, eggs, erddap, fatty, Fatty_acid, female, latitude, longitude, management, oceanography, office, preliminary, relative, Relative_concentration_eggs, Relative_concentration_spawning_adult, site, spawning, species, taxonomy, time
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/719379 (external link)
attribute NC_GLOBAL Northernmost_Northing double 41.32
attribute NC_GLOBAL param_mapping String {'719379': {'Lat': 'master - latitude', 'Lon': 'master - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/719379/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Connecticut
attribute NC_GLOBAL people_0_affiliation_acronym String UConn
attribute NC_GLOBAL people_0_person_name String Hannes Baumann
attribute NC_GLOBAL people_0_person_nid String 528586
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Stony Brook University - SoMAS
attribute NC_GLOBAL people_1_affiliation_acronym String SUNY-SB SoMAS
attribute NC_GLOBAL people_1_person_name String Janet Nye
attribute NC_GLOBAL people_1_person_nid String 650186
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of Connecticut
attribute NC_GLOBAL people_2_affiliation_acronym String UConn
attribute NC_GLOBAL people_2_person_name String Hannes Baumann
attribute NC_GLOBAL people_2_person_nid String 528586
attribute NC_GLOBAL people_2_role String Contact
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Hannah Ake
attribute NC_GLOBAL people_3_person_nid String 650173
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish
attribute NC_GLOBAL projects_0_acronym String HYPOA
attribute NC_GLOBAL projects_0_description String Description from NSF award abstract:
Coastal marine ecosystems provide a number of important services and resources for humans, and at the same time, coastal waters are subject to environmental stressors such as increases in ocean acidification and reductions in dissolved oxygen. The effects of these stressors on coastal marine organisms remain poorly understood because most research to date has examined the sensitivity of species to one factor, but not to more than one in combination. This project will determine how a model fish species, the Atlantic silverside, will respond to observed and predicted levels of dissolved carbon dioxide (CO2) and oxygen (O2). Shorter-term experiments will measure embryo and larval survival, growth, and metabolism, and determine whether parents experiencing stressful conditions produce more robust offspring. Longer-term experiments will study the consequences of ocean acidification over the entire life span by quantifying the effects of high-CO2 conditions on the ratio of males to females, lifetime growth, and reproductive investment. These studies will provide a more comprehensive view of how multiple stressors may impact populations of Atlantic silversides and potentially other important forage fish species. This collaborative project will support and train three graduate students at the University of Connecticut and the Stony Brook University (NY), two institutions that attract students from minority groups. It will also provide a variety of opportunities for undergraduates to participate in research and the public to learn about the study, through summer research projects, incorporation in the "Women in Science and Engineering" program, and interactive displays of environmental data from monitoring buoys. The two early-career investigators are committed to increasing ocean literacy and awareness of NSF-funded research through public talks and presentations.
This project responds to the recognized need for multi-stressor assessments of species sensitivities to anthropogenic environmental change. It will combine environmental monitoring with advanced experimental approaches to characterize early and whole life consequences of acidification and hypoxia in the Atlantic silverside (Menidia menidia), a valued model species and important forage fish along most of the US east coast. Experiments will employ a newly constructed, computer-controlled fish rearing system to allow independent and combined manipulation of seawater pCO2 and dissolved oxygen (DO) content and the application of static and fluctuating pCO2 and DO levels that were chosen to represent contemporary and potential future scenarios in productive coastal habitats. First CO2, DO, and CO2 � DO dependent reaction norms will be quantified for fitness-relevant early life history (ELH) traits including pre- and post-hatch survival, time to hatch, post-hatch growth, by rearing offspring collected from wild adults from fertilization to 20 days post hatch (dph) using a full factorial design of 3 CO2 � 3 DO levels. Second, the effects of tidal and diel CO2 � DO fluctuations of different amplitudes on silverside ELH traits will be quantified. To address knowledge gaps regarding the CO2-sensitivity in this species, laboratory manipulations of adult spawner environments and reciprocal offspring exposure experiments will elucidate the role of transgenerational plasticity as a potential short-term mechanism to cope with changing environments. To better understand the mechanisms of fish early life CO2-sensitivity, the effects of temperature � CO2 on pre- and post-hatch metabolism will be robustly quantified. The final objective is to rear silversides from fertilization to maturity under different CO2 levels and assess potential CO2-effects on sex ratio and whole life growth and fecundity.
Related references:
Gobler, C.J. and Baumann, H. (2016) Hypoxia and acidification in ocean ecosystems: Coupled dynamics and effects on marine life. Biology Letters 12:20150976. doi:10.1098/rsbl.2015.0976
Baumann, H. (2016) Combined effects of ocean acidification, warming, and hypoxia on marine organisms. Limnology and Oceanography e-Lectures 6:1-43. doi:10.1002/loe2.10002
Depasquale, E., Baumann, H., and Gobler, C.J. (2015) Variation in early life stage vulnerability among Northwest Atlantic estuarine forage fish to ocean acidification and low oxygen Marine Ecology Progress Series 523: 145–156.doi:10.3354/meps11142
attribute NC_GLOBAL projects_0_end_date String 2018-11
attribute NC_GLOBAL projects_0_geolocation String Eastern Long Island Sound, CT, USA
attribute NC_GLOBAL projects_0_name String Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish
attribute NC_GLOBAL projects_0_project_nid String 650184
attribute NC_GLOBAL projects_0_start_date String 2015-12
attribute NC_GLOBAL publisher_name String Hannah Ake
attribute NC_GLOBAL publisher_role String BCO-DMO Data Manager(s)
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 41.32
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v29
attribute NC_GLOBAL subsetVariables String Species, Collection_site, latitude, longitude
attribute NC_GLOBAL summary String Gas chromatography was used to quantify the absolute (mg g dry weight-1) and
relative concentrations (% of total) of 27 FAs for each of 5 females (whole
individual) and their unfertilized eggs (~ 1 ml). Samples were first dried and
then homogenized in a solution of chloroform-methanol (2:1 v/v) and
tricosanoic acid (23:0) as an internal standard for quantification of mg
g\u22121\u00a0dry mass of fatty acids. Lipids were cold-extracted from
approximately 50 mg of dry mass. A Shimadzu GC-2014 gas chromatograph set with
a Phenomenex ZB-WAX plus capillary column (30\u00a0m long; 0.53 mm ID; 1.0 um
thick) was used to quantify FAs, and individual FAs were identified by
comparison to commercial standards (Supelco, Inc).\u00a0

These data are\u00a0associated with the corresponding paper:

[Snyder, J.T.*, Murray, C.S.*, and Baumann, H. (2017) Potential for maternal
effects on offspring CO2-sensitivities in a coastal marine fish. Journal of
Experimental Marine Biology and Ecology (in

Other datasets related to this paper:

[Survival, length, and growth responses of M. menidia offspring from different
females exposed to contrasting CO2 environments.](\\https://www.bco-
attribute NC_GLOBAL title String Fatty acid profiles of M. menidia females and their unfertilized eggs.
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -72.02
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5-beta
variable Species   String  
attribute Species description String Atlantic silverside; Menidia menidia
attribute Species ioos_category String Taxonomy
attribute Species long_name String Species
attribute Species units String unitless
variable Collection_site   String  
attribute Collection_site description String Mumford Cove Connecticut USA
attribute Collection_site ioos_category String Unknown
attribute Collection_site long_name String Collection Site
attribute Collection_site units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 41.32, 41.32
attribute latitude axis String Y
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude of field collection site
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -72.02, -72.02
attribute longitude axis String X
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude of field collection site
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable Collection_date   String  
attribute Collection_date description String Date of field collection for the spawners used in the experiment; YYYY/MM/DD
attribute Collection_date ioos_category String Time
attribute Collection_date long_name String Collection Date
attribute Collection_date source_name String Collection_date
attribute Collection_date units String unitless
variable Fatty_acid   String  
attribute Fatty_acid description String Individual fatty acid nomenclature
attribute Fatty_acid ioos_category String Unknown
attribute Fatty_acid long_name String Fatty Acid
attribute Fatty_acid units String unitless
variable Female   String  
attribute Female description String Five females denoted by letter A B C D E
attribute Female ioos_category String Unknown
attribute Female long_name String Female
attribute Female units String unitless
variable Absolute_concentration_spawning_adult   float  
attribute Absolute_concentration_spawning_adult _FillValue float NaN
attribute Absolute_concentration_spawning_adult actual_range float 0.0, 20.4
attribute Absolute_concentration_spawning_adult description String Absolute fatty acid concentration in mg per g of dry weight
attribute Absolute_concentration_spawning_adult ioos_category String Unknown
attribute Absolute_concentration_spawning_adult long_name String Absolute Concentration Spawning Adult
attribute Absolute_concentration_spawning_adult units String milligram per gram
variable Absolute_concentration_eggs   float  
attribute Absolute_concentration_eggs _FillValue float NaN
attribute Absolute_concentration_eggs actual_range float 0.0, 47.6
attribute Absolute_concentration_eggs description String Absolute fatty acid concentration in mg per g of dry weight
attribute Absolute_concentration_eggs ioos_category String Biology
attribute Absolute_concentration_eggs long_name String Absolute Concentration Eggs
attribute Absolute_concentration_eggs units String milligram per gram
variable Relative_concentration_spawning_adult   float  
attribute Relative_concentration_spawning_adult _FillValue float NaN
attribute Relative_concentration_spawning_adult actual_range float 0.0, 36.4
attribute Relative_concentration_spawning_adult description String Relative fatty acid concentration in percent of total fatty concentration
attribute Relative_concentration_spawning_adult ioos_category String Unknown
attribute Relative_concentration_spawning_adult long_name String Relative Concentration Spawning Adult
attribute Relative_concentration_spawning_adult units String percent
variable Relative_concentration_eggs   float  
attribute Relative_concentration_eggs _FillValue float NaN
attribute Relative_concentration_eggs actual_range float 0.0, 29.8
attribute Relative_concentration_eggs description String Relative fatty acid concentration in percent of total fatty concentration
attribute Relative_concentration_eggs ioos_category String Biology
attribute Relative_concentration_eggs long_name String Relative Concentration Eggs
attribute Relative_concentration_eggs units String percent

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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