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   set  data   graph     files  public [MCR_HIGH RES_DOC] - High Resolution sampling of dissolved organic matter (DOM) around Mo'
orea coupled with macroalgal collections (Coral DOM2 project) (Collaborative Research:
Dissolved organic matter feedbacks in coral reef resilience: The genomic & geochemical basis
for microbial modulation of algal phase shifts)
   ?   F   I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_720234

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson,.odvTxt
attribute NC_GLOBAL acquisition_description String Procedural overview:

\- All samples were collected via boat, using 1L square Polycarbonate bottles
(surface grabs).
- Bottles were gravity filtered (combusted 47mm GF/F) into glass vials.
- Samples were returned to the on-shore MCR LTER laboratory, acidified and
stored at Room Temperature.
- Samples were shipped to Craig Carlson\u2019s Laboratory at UCSB for
analysis using the HTCO Method (Carlson, et al. 2010 DSRII).

DOC analysis methodology\u00a0(from Carlson et al (2010)).

All samples were analyzed via high-temperature combustion on Shimadzu TOC-V
analyzers that were slightly modified from the manufacturer\u2019s model
system. The condensation coil was removed and the head space of an internal
water trap was reduced to minimize system dead space. The combustion tube
contained 0.5 cm Pt pillows placed on top of Pt alumina beads to improve peak
shape and to reduce alteration of the combustion matrix throughout the
analytical run. CO2-free carrier gas was delivered to the TOC-V systems via
commercial ultra high purity gas cylinders or a Whatmans gas generator. Three
milliliters of sample were drawn into a 5 ml injection syringe, acidified with
2 M HCL (1.5%), and sparged for 1.5 min with CO2-free gas. Three to five
replicate 100 ml of sample were injected into the combustion tube heated to
680 1C. A magnesium perchlorate trap was added to the existing water and
halide traps to ensure removal of water vapor from the gas line prior to
entering a nondispersive infrared detector. The resulting peak area was
integrated with Shimadzu chromatographic software.
Extensive conditioning of the combustion tube with repeated injections of
low carbon water (LCW) and deep seawater was essential to minimize the machine
blanks. The system response was standardized daily with a four-point
calibration curve of potassium hydrogen phthalate solution in LCW. Sample and
reference swapping and intercalibration exercises were conducted periodically
between the UCSB and University of Miami to ensure comparability between
sample sets. All samples were systematically referenced against low carbon
water, deep Sargasso Sea reference waters (2600 m), and surface Sargasso Sea
water every 6-8 analyses (Hansell and Carlson, 1998; Carlson et al., 2004).
Daily reference waters were calibrated with DOC Consensus Reference Waters
(Hansell, 2005). The standard deviation of the deep and surface references
analyzed throughout a run generally had a coefficient of variation ranging
between 1-2% over the 3-7 independent analyses (number of references depended
on the size of the run), allowing resolution of approximately 1 mmol/kg in the
deep waters.
attribute NC_GLOBAL awards_0_award_nid String 675024
attribute NC_GLOBAL awards_0_award_number String OCE-1538567
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538567 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL awards_1_award_nid String 675030
attribute NC_GLOBAL awards_1_award_number String OCE-1538393
attribute NC_GLOBAL awards_1_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538393 (external link)
attribute NC_GLOBAL awards_1_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_1_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_1_funding_source_nid String 355
attribute NC_GLOBAL awards_1_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_1_program_manager_nid String 50446
attribute NC_GLOBAL awards_2_award_nid String 675035
attribute NC_GLOBAL awards_2_award_number String OCE-1538428
attribute NC_GLOBAL awards_2_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538428 (external link)
attribute NC_GLOBAL awards_2_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_2_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_2_funding_source_nid String 355
attribute NC_GLOBAL awards_2_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_2_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String MCR_HIGH RES_DOM
High Resolution sampling of dissolved organic matter (DOM) around Mo'orea coupled with macroalgal collections, July-Sept 2016
C. Carlson (UCSB)
version: 2017-12-01
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2017-12-01T20:17:12Z
attribute NC_GLOBAL date_modified String 2018-12-17T19:32:35Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.720234.1
attribute NC_GLOBAL Easternmost_Easting double -149.7576461
attribute NC_GLOBAL geospatial_lat_max double -17.47187021
attribute NC_GLOBAL geospatial_lat_min double -17.60410798
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -149.7576461
attribute NC_GLOBAL geospatial_lon_min double -149.9240371
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 1.0
attribute NC_GLOBAL geospatial_vertical_min double 1.0
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/720234 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String TOC analyzer
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 720279
attribute NC_GLOBAL instruments_0_description String A unit that accurately determines the carbon concentrations of organic compounds typically by detecting and measuring its combustion product (CO2). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/bs116.pdf
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB04/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Total Organic Carbon Analyzer
attribute NC_GLOBAL instruments_0_instrument_nid String 652
attribute NC_GLOBAL instruments_0_supplied_name String Shimadzu TOC-V analyzers, modified
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, chemical, commerce, cruise, data, dataset, date, department, depth, dmo, doc, DOC_FINAL_uM, DOC_SD, erddap, final, flag, iso, latitude, local, longitude, management, oceanography, office, preliminary, sample, SAMPLE_ID, station, time, type, u
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/720234/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/720234 (external link)
attribute NC_GLOBAL Northernmost_Northing double -17.47187021
attribute NC_GLOBAL param_mapping String {'720234': {'Latitude': 'flag - latitude', 'Depth': 'flag - depth', 'Longitude': 'flag - longitude', 'ISO_DateTime_Local': 'flag - time'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/720234/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of California-Santa Barbara
attribute NC_GLOBAL people_0_affiliation_acronym String UCSB-MSI
attribute NC_GLOBAL people_0_person_name String Craig Carlson
attribute NC_GLOBAL people_0_person_nid String 50575
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Nancy Copley
attribute NC_GLOBAL people_1_person_nid String 50396
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String Coral DOM2
attribute NC_GLOBAL projects_0_acronym String Coral DOM2
attribute NC_GLOBAL projects_0_description String NSF award abstract:
Coral reef degradation, whether driven by overfishing, nutrient pollution, declining water quality, or other anthropogenic factors, is associated with a phase shift towards a reefs dominated by fleshy algae. In many cases managing and ameliorating these stressors does not lead to a return to coral dominance, and reefs languish in an algal-dominated state for years. Nearly a decade of research has demonstrated that trajectories toward increasing algal dominance are restructuring microbial community composition and metabolism; the investigators hypothesize that microbial processes facilitate the maintenance of algal dominance by metabolizing organic compounds released by algae thereby stressing corals through hypoxia and disease. The resilience of reefs to these phase shifts is a critical question in coral reef ecology, and managing reefs undergoing these community shifts requires developing an understanding of the role of microbial interactions in facilitating algal overgrowth and altering reef ecosystem function. The research proposed here will investigate the organics produced by algae, the microbes that metabolize the organics, and the impacts of these processes on coral health and growth. This research has implications for managing reef resilience to algal phase shifts by testing the differential resistance of coral-associated microbial communities to algae and defining thresholds of algal species cover which alter ecosystem biogeochemistry. This project provides mentoring across multiple career levels, linking underrepresented undergraduates, two graduate students, a postdoctoral researcher, and a beginning and established investigators.
This project will integrate dissolved organic matter (DOM) geochemistry, microbial genomics and ecosystem process measurements at ecologically-relevant spatial and temporal scales to test hypothetical mechanisms by which microbially-mediated feedbacks may facilitate the spread of fleshy algae on Pacific reef ecosystems. A key product of this research will be understanding how the composition of corals and algae on reefs interact synergistically with complex microbial communities to influence reef ecosystem resilience to algal phase shifts. Emerging molecular and biogeochemical methods will be use to investigate mechanisms of microbial-DOM interactions at multiple spatial and temporal scales. This project will leverage the background environmental data, laboratory facilities and field logistical resources of the Mo'orea Coral Reef Long Term Ecological Research Project in French Polynesia and contribute to the mission of that program of investigating coral reef resilience in the face of global change. The investigators will quantify bulk diel patterns of DOM production and characterize the composition of chromophoric components and both free and acid-hydrolyzable neutral monosaccharides and amino acids from varying benthic algae sources. The team will also characterize planktonic and coral-associated microbial community changes in taxonomic composition and gene expression caused by algal DOM amendments in on-site controlled environmental chambers using phylogenetics and metatranscriptomics, including tracking algal exudate utilization by specific microbial lineages. Field-deployed 100 liter tent mesocosms will be used to examine in situ diel patterns of coupled DOM production and consumption, microbial community genomics and ecosystem metabolism over representative benthic communities comprising combinations of algal and coral species. Together these experimental results will guide interpretation of field surveys of centimeter-scale spatial dynamics of planktonic and coral-associated microbial genomics and metabolism at zones of coral-algal interaction, including boundary layer dynamics of oxygen, bacteria and DOM using planar optodes, high-throughput flow cytometry and fluorescence spectroscopy.
attribute NC_GLOBAL projects_0_end_date String 2018-11
attribute NC_GLOBAL projects_0_geolocation String Pacific Coral Reefs
attribute NC_GLOBAL projects_0_name String Collaborative Research: Dissolved organic matter feedbacks in coral reef resilience: The genomic & geochemical basis for microbial modulation of algal phase shifts
attribute NC_GLOBAL projects_0_project_nid String 675025
attribute NC_GLOBAL projects_0_start_date String 2015-12
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double -17.60410798
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String CRUISE,TYPE,depth
attribute NC_GLOBAL summary String Data was collected here in collaboration with the MCR-LTER group in July 2016. Dissolved organic carbon (DOC) samples were collected for us during an existing field project (Turbinaria tissue collections). The primary goal was to collect water from as many of ~180 Lagoon sites as possible to obtain high-resolution surface DOC dataset. This dataset includes dissolved organic carbon (DOC) measurements around the periphery of Mo'orea Island from July 26 to September 1, 2016. Samples were taken at 1-meter depth.
attribute NC_GLOBAL time_coverage_end String 2016-09-01T10:29:00Z
attribute NC_GLOBAL time_coverage_start String 2016-07-26T11:20:00Z
attribute NC_GLOBAL title String [MCR_HIGH RES_DOC] - High Resolution sampling of dissolved organic matter (DOM) around Mo'orea coupled with macroalgal collections (Coral DOM2 project) (Collaborative Research: Dissolved organic matter feedbacks in coral reef resilience: The genomic & geochemical basis for microbial modulation of algal phase shifts)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -149.9240371
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable SAMPLE_ID   short  
attribute SAMPLE_ID _FillValue short 32767
attribute SAMPLE_ID actual_range short 1, 320
attribute SAMPLE_ID bcodmo_name String sample
attribute SAMPLE_ID description String Unique Identified Code for each sample collected
attribute SAMPLE_ID long_name String SAMPLE ID
attribute SAMPLE_ID nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute SAMPLE_ID units String unitless
variable CRUISE   String  
attribute CRUISE bcodmo_name String cruise_id
attribute CRUISE description String cruise name
attribute CRUISE long_name String CRUISE
attribute CRUISE units String unitless
variable STATION   String  
attribute STATION bcodmo_name String station
attribute STATION description String MCR site number
attribute STATION long_name String STATION
attribute STATION units String unitless
variable TYPE   String  
attribute TYPE bcodmo_name String sample_type
attribute TYPE description String B for bottle - surface bottle grabs
attribute TYPE long_name String TYPE
attribute TYPE units String unitless
variable time   double  
attribute time _CoordinateAxisType String Time
attribute time actual_range double 1.469532E9, 1.47272574E9
attribute time axis String T
attribute time bcodmo_name String ISO_DateTime_Local
attribute time description String Local date and time (UTC-10) in the format yyyy-mm-ddThh:mm:ss
attribute time ioos_category String Time
attribute time long_name String ISO Date Time Local
attribute time source_name String ISO_DateTime_Local
attribute time standard_name String time
attribute time time_origin String 01-JAN-1970 00:00:00
attribute time time_precision String 1970-01-01T00:00:00Z
attribute time units String seconds since 1970-01-01T00:00:00Z
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double -17.60410798, -17.47187021
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude; north is positive
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -149.9240371, -149.7576461
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude; east is positive
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable depth   double  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth _FillValue double NaN
attribute depth actual_range double 1.0, 1.0
attribute depth axis String Z
attribute depth bcodmo_name String depth
attribute depth colorBarMaximum double 8000.0
attribute depth colorBarMinimum double -8000.0
attribute depth colorBarPalette String TopographyDepth
attribute depth description String Sampling depth
attribute depth ioos_category String Location
attribute depth long_name String Depth
attribute depth nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable DOC_FINAL_uM   float  
attribute DOC_FINAL_uM _FillValue float NaN
attribute DOC_FINAL_uM actual_range float 60.12, 79.45
attribute DOC_FINAL_uM bcodmo_name String DOC
attribute DOC_FINAL_uM description String Dissolved organic carbon concentration by HTCO. Glass fiber filtrate type GF/F (Whatman). Methodological reference is Carlson et al. 2010 DSRII
attribute DOC_FINAL_uM long_name String DOC FINAL U M
attribute DOC_FINAL_uM nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CORGZZZX/ (external link)
attribute DOC_FINAL_uM units String Micromol Carbon
variable DOC_SD   float  
attribute DOC_SD _FillValue float NaN
attribute DOC_SD actual_range float 0.0, 4.54
attribute DOC_SD bcodmo_name String DOC
attribute DOC_SD colorBarMaximum double 50.0
attribute DOC_SD colorBarMinimum double 0.0
attribute DOC_SD description String standard deviation for DOC
attribute DOC_SD long_name String DOC SD
attribute DOC_SD nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CORGZZZX/ (external link)
attribute DOC_SD units String Micromol Carbon
variable FLAG   byte  
attribute FLAG _FillValue byte 127
attribute FLAG actual_range byte 1, 4
attribute FLAG bcodmo_name String q_flag
attribute FLAG colorBarMaximum double 150.0
attribute FLAG colorBarMinimum double 0.0
attribute FLAG description String quality flag for DOC [1= acceptable; 4= questionable]
attribute FLAG long_name String FLAG
attribute FLAG units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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