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ERDDAP > info > bcodmo_dataset_722433

Title Sum-
Institution Dataset ID
   set  data   graph     files  public Open Mesocosm Oyster 15N2 and N2O    ?     I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_722433

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String 15N labeled phytoplankton was continuously pumped for three days into 30.5 cm
diameter mesocosms containing\u00a0 10 cm of sediment and 20 liters of
overlying water. Three mesocosms contained oysters twelve 7-cm long oysters
(O1, O2, O2) and three mesocosms had no oysters and served as controls
(C1,C2,C3). Mesoscosms were flow through with a 0.3 day residence time and
open to the atmosphere. N2O and\u00a015N2\u00a0were measured during the
phytoplankton addition period.

Water samples for N2O analysis were collected with a peristaltic pump through
a syringe needle directly into 12 ml exetainer that had been flushed with N2
and preserved with KOH to a pH above 12. Approximately six ml sample was
collected. N2O concentrations in the headspace were measured on a GC-
ECD.\u00a0 Water samples for 15N2 samples were collected with a peristaltic
pump through a syringe needle directly into 30 ml serum bottles that had been
flushed with He and preserved with KOH to a pH above 12. \u00a0 Approximately
eight ml sample was collected. 15N2 was analyzed by GC - Isotope Ratio Mass
Spectrometry (IRMS). 15N-Phyto sample was collected by GFF 0.7um filtration of
phytoplankton stock solution.
attribute NC_GLOBAL awards_0_award_nid String 568863
attribute NC_GLOBAL awards_0_award_number String OCE-1233372
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1233372 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Open Mesocosm Oyster Experiment 15N2 and N2O
PI: Craig Tobias
Co-PI: Bongkuen Song, Michael Piehler, Mark Brush
Version: 20171231
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2018-01-05T18:50:20Z
attribute NC_GLOBAL date_modified String 2019-05-07T17:57:33Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.722433.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/722433 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String IR Mass Spec
attribute NC_GLOBAL instruments_0_dataset_instrument_description String 15N2 was measured on a Thermo Delta V IRMS fitted with a Gas Bench II interface following separation from O2 and Ar on a mol sieve 5A column.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 722450
attribute NC_GLOBAL instruments_0_description String The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB16/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Isotope-ratio Mass Spectrometer
attribute NC_GLOBAL instruments_0_instrument_nid String 469
attribute NC_GLOBAL instruments_0_supplied_name String Thermo Delta V IRMS
attribute NC_GLOBAL instruments_1_acronym String Gas Chromatograph
attribute NC_GLOBAL instruments_1_dataset_instrument_description String N2O was measured on a Agilent 7890B GC with a Poropak Column.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 722452
attribute NC_GLOBAL instruments_1_description String Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB02/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Gas Chromatograph
attribute NC_GLOBAL instruments_1_instrument_nid String 661
attribute NC_GLOBAL instruments_1_supplied_name String Agilent 7890B GC with a Poropak Column
attribute NC_GLOBAL instruments_2_dataset_instrument_description String 15N Phyto sample  was analyzed on the IRMS coupled to a Costech Elemental Analyzer.
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 722451
attribute NC_GLOBAL instruments_2_description String Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material.
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB01/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Elemental Analyzer
attribute NC_GLOBAL instruments_2_instrument_nid String 546339
attribute NC_GLOBAL instruments_2_supplied_name String Costech Elemental Analyzer
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, C1_15N2, C1_N2O, C2_15N2, C2_N2O, C3_15N2, C3_N2O, chemical, data, dataset, dmo, erddap, hours, management, O1_15N2, O1_N2O, O2, O2_15N2, O2_N2O, O3_15N2, O3_N2O, oceanography, office, oxygen, phyto, Phyto_15N, preliminary
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/722433/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/722433 (external link)
attribute NC_GLOBAL param_mapping String {'722433': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/722433/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Connecticut
attribute NC_GLOBAL people_0_affiliation_acronym String UConn - Avery Point
attribute NC_GLOBAL people_0_person_name String Craig Tobias
attribute NC_GLOBAL people_0_person_nid String 51731
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Virginia Institute of Marine Science
attribute NC_GLOBAL people_1_affiliation_acronym String VIMS
attribute NC_GLOBAL people_1_person_name String Mark J. Brush
attribute NC_GLOBAL people_1_person_nid String 568861
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of North Carolina at Chapel Hill
attribute NC_GLOBAL people_2_affiliation_acronym String UNC-Chapel Hill-IMS
attribute NC_GLOBAL people_2_person_name String Michael F. Piehler
attribute NC_GLOBAL people_2_person_nid String 522931
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Virginia Institute of Marine Science
attribute NC_GLOBAL people_3_affiliation_acronym String VIMS
attribute NC_GLOBAL people_3_person_name String Bongkeun Song
attribute NC_GLOBAL people_3_person_nid String 51729
attribute NC_GLOBAL people_3_role String Co-Principal Investigator
attribute NC_GLOBAL people_3_role_type String originator
attribute NC_GLOBAL people_4_affiliation String University of Connecticut
attribute NC_GLOBAL people_4_affiliation_acronym String UConn - Avery Point
attribute NC_GLOBAL people_4_person_name String Craig Tobias
attribute NC_GLOBAL people_4_person_nid String 51731
attribute NC_GLOBAL people_4_role String Contact
attribute NC_GLOBAL people_4_role_type String related
attribute NC_GLOBAL people_5_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_5_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_5_person_name String Mathew Biddle
attribute NC_GLOBAL people_5_person_nid String 708682
attribute NC_GLOBAL people_5_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_5_role_type String related
attribute NC_GLOBAL project String Oyster Reef N2O Emission
attribute NC_GLOBAL projects_0_acronym String Oyster Reef N2O Emission
attribute NC_GLOBAL projects_0_description String Extracted from the NSF award abstract:
Oyster reefs are biogeochemical hot spots and prominent estuarine habitats that provide disproportionate ecological function. Suspension-feeding eastern oysters, Crassostrea virginica, are capable of improving water quality and diminishing eutrophication by filtering nutrients and particles from the water and depositing them in the sediments. Remineralization of these deposits may enhance sedimentary denitrification that facilitates nitrogen removal in tidal estuaries. However, the scientific underpinning of oyster reef function has been challenged in various studies. In addition, recent studies of filter feeding invertebrates reported the production of nitrous oxide (N2O), a greenhouse gas, as an end product of incomplete denitrification by gut microbes. C. virginica could be another source of N2O flux from intertidal habitats. Preliminary work indicated substantial N2O production from individual oysters. The estimated N2O production from high density oyster reefs may exceed the N2O flux measured from some estuaries. With the new discovery of N2O emission and uncertainty regarding eutrophication control, the ecological value of oyster reef restoration may become equivocal.
This project will quantify N2O fluxes to understand the factors controlling N2O emission from oyster reefs. Sedimentary N processes will be examined to develop an oyster reef N model to estimate N2O emission from tidal creek estuaries relative to other N cycling processes. The PIs hypothesize that intertidal oyster reefs are a substantial source of N2O emission from estuarine ecosystems and the magnitude of emission may be linked to water quality. If substantial N2O flux from oyster reefs is validated, ecological benefits of oyster reef restoration should be reevaluated. This interdisciplinary research team includes a microbial ecologist, a biogeochemist, an ecologist and an ecosystem modeler. They will utilize stable isotope and molecular microbiological techniques to quantify oyster N2O production, elucidate microbial sources of N2O emission from oysters and sediments, and estimate seasonal variation of N2O fluxes from oyster reefs. Measurements from this study will be integrated into a coupled oyster bioenergetics-sediment biogeochemistry model to compare system level rates of N cycling on oyster reefs as a function of oyster density and water quality. Modeling results will be used to assess the relative trade-­offs of oyster restoration associated with N cycling. They expect to deliver the following end products:1) estimation of annual N2O flux from oyster reefs as an additional source of greenhouse gases from estuaries, 2) a better understanding of the environmental and microbial factors influencing N2O and N2 fluxes in tidal estuaries, 3) transformative knowledge for the effect of oyster restoration on water quality enhancement and ecosystem function, 4) direct guidance for oyster restoration projects whose goals include water quality enhancement, and 5) a modeling tool for use in research and restoration planning.
attribute NC_GLOBAL projects_0_end_date String 2015-08
attribute NC_GLOBAL projects_0_name String Microbial Regulation of Greenhouse Gas N2O Emission from Intertidal Oyster Reefs
attribute NC_GLOBAL projects_0_project_nid String 527289
attribute NC_GLOBAL projects_0_start_date String 2012-12
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String Phyto_15N
attribute NC_GLOBAL summary String Open Mesocosm Oyster 15N2 and N2O
attribute NC_GLOBAL title String Open Mesocosm Oyster 15N2 and N2O
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Hours   float  
attribute Hours _FillValue float NaN
attribute Hours actual_range float 0.0, 64.58
attribute Hours bcodmo_name String duration
attribute Hours description String Time since phyto feeding start
attribute Hours long_name String Hours
attribute Hours units String hours
variable Phyto_15N   byte  
attribute Phyto_15N _FillValue byte 127
attribute Phyto_15N actual_range byte 45, 45
attribute Phyto_15N bcodmo_name String unknown
attribute Phyto_15N description String 15N enrichment of phytoplankton at% 15N
attribute Phyto_15N long_name String Phyto 15 N
attribute Phyto_15N units String percent
variable O1_15N2   float  
attribute O1_15N2 _FillValue float NaN
attribute O1_15N2 actual_range float 0.5, 23.3
attribute O1_15N2 bcodmo_name String N
attribute O1_15N2 description String Oyster mecosm1 15N enrichment of dissolved N2
attribute O1_15N2 long_name String O1 15 N2
attribute O1_15N2 units String permil (/ml)
variable O1_N2O   float  
attribute O1_N2O _FillValue float NaN
attribute O1_N2O actual_range float 19.3, 39.8
attribute O1_N2O bcodmo_name String Nitrous Oxide
attribute O1_N2O description String Oyster mecosm1 Aqueous N2O concentration
attribute O1_N2O long_name String O1 N2 O
attribute O1_N2O units String nanomole (nM)
variable O2_15N2   float  
attribute O2_15N2 _FillValue float NaN
attribute O2_15N2 actual_range float 0.4, 44.7
attribute O2_15N2 bcodmo_name String N
attribute O2_15N2 description String Oyster mecosm 2 15N enrichment of dissolved N2
attribute O2_15N2 long_name String O2 15 N2
attribute O2_15N2 units String permil (/ml)
variable O2_N2O   float  
attribute O2_N2O _FillValue float NaN
attribute O2_N2O actual_range float 30.1, 48.8
attribute O2_N2O bcodmo_name String Nitrous Oxide
attribute O2_N2O description String Oyster mecosm 2 Aqueous N2O concentration
attribute O2_N2O long_name String O2 N2 O
attribute O2_N2O units String nanomole (nM)
variable O3_15N2   float  
attribute O3_15N2 _FillValue float NaN
attribute O3_15N2 actual_range float 0.4, 34.3
attribute O3_15N2 bcodmo_name String N
attribute O3_15N2 description String Oyster mecosm 3 15N enrichment of dissolved N2
attribute O3_15N2 long_name String O3 15 N2
attribute O3_15N2 units String permil (/ml)
variable O3_N2O   float  
attribute O3_N2O _FillValue float NaN
attribute O3_N2O actual_range float 14.7, 34.0
attribute O3_N2O bcodmo_name String Nitrous Oxide
attribute O3_N2O description String Oyster mecosm 3 Aqueous N2O concentration
attribute O3_N2O long_name String O3 N2 O
attribute O3_N2O units String nanomole (nM)
variable C1_15N2   float  
attribute C1_15N2 _FillValue float NaN
attribute C1_15N2 actual_range float 0.4, 25.0
attribute C1_15N2 bcodmo_name String N
attribute C1_15N2 description String Control mecosm 1 15N enrichment of dissolved N2
attribute C1_15N2 long_name String C1 15 N2
attribute C1_15N2 units String permil (/ml)
variable C1_N2O   float  
attribute C1_N2O _FillValue float NaN
attribute C1_N2O actual_range float 14.6, 37.2
attribute C1_N2O bcodmo_name String Nitrous Oxide
attribute C1_N2O description String Control mecosm 1 Aqueous N2O concentration
attribute C1_N2O long_name String C1 N2 O
attribute C1_N2O units String nanomole (nM)
variable C2_15N2   float  
attribute C2_15N2 _FillValue float NaN
attribute C2_15N2 actual_range float 0.2, 24.7
attribute C2_15N2 bcodmo_name String N
attribute C2_15N2 description String Control mecosm 2 15N enrichment of dissolved N2
attribute C2_15N2 long_name String C2 15 N2
attribute C2_15N2 units String permil (/ml)
variable C2_N2O   float  
attribute C2_N2O _FillValue float NaN
attribute C2_N2O actual_range float 15.5, 30.9
attribute C2_N2O bcodmo_name String Nitrous Oxide
attribute C2_N2O description String Control mecosm 2 Aqueous N2O concentration
attribute C2_N2O long_name String C2 N2 O
attribute C2_N2O units String nanomole (nM)
variable C3_15N2   float  
attribute C3_15N2 _FillValue float NaN
attribute C3_15N2 actual_range float 0.3, 20.6
attribute C3_15N2 bcodmo_name String N
attribute C3_15N2 description String Control mecosm 3 15N enrichment of dissolved N2
attribute C3_15N2 long_name String C3 15 N2
attribute C3_15N2 units String permil (/ml)
variable C3_N2O   float  
attribute C3_N2O _FillValue float NaN
attribute C3_N2O actual_range float 29.2, 67.2
attribute C3_N2O bcodmo_name String Nitrous Oxide
attribute C3_N2O description String Control mecosm 3 Aqueous N2O concentration
attribute C3_N2O long_name String C3 N2 O
attribute C3_N2O units String nanomole (nM)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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