ERDDAP > info > bcodmo_dataset_723266

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	Photophysiological responses of two dinoflagellate species used in natural high light exposure experiments (Protist Signaling project)		I   M	background			BCO-DMO	bcodmo_dataset_723266

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Irradiance exposure experiments:\u00a0 Experiments to test the light stress response consisted of exposing dinoflagellates to natural sunlight and monitoring a range of physiological and biochemical responses. Sunlight exposure took place in unshaded outdoor Plexiglas tanks (without lids), supplied with a constant flow of seawater to maintain incubation bottles approximate growth temperatures of 15-16\u00b0C for the duration of each experimental exposure period. A Li-Cor LI-1400 data logger with a 2\u03c0 sensor collected incident PAR data; irradiance received during incubation period is presented as total dose (mol photons m-2) and (for Fv/Fm data) as the maximum instantaneous irradiance recorded during the exposure period (\u00b5mol photons m-2 s-1). To initiate experiments, dinoflagellate cultures were combined and divided into 250-mL polycarbonate bottles, which block penetration of UVR. Bottles were returned to the growth incubator for approximately 1 h acclimation before the experiment began. After acclimation, \u201cpre-exposure\u201d samples were collected from each bottle for determination of morphological, biochemical, and physiological characteristics of dinoflagellate cells. Bottles were then covered in treatment-specific neutral density screening as needed to achieve desired irradiances, and placed in the outdoor tank to begin the light exposure period. Control bottles were covered with enough screen layers to approximate growth incubator irradiances. Experiments included four replicates each of either two or three treatments: all experiments included \u201chigh PAR\u201d and \u201ccontrol\u201d treatments, while A2 and H2 also included \u201cmoderate PAR\u201d. Exposure duration varied slightly by experiment but was approximately 1.5 h. Photosynthetic efficiency Fv/Fm (unitless) was measured at 15, 25, or 30 min time intervals during the exposure period, depending on the experiment. After the exposure period, all bottles were taken indoors for a second round of sampling (henceforth referred to as \u201cpost-exposure\u201d). Bottles were then rid of any screening and returned to the growth incubator for the recovery period (1.5-2 h depending on experiment). After recovery a third round of samples (termed \u201cpost-recovery\u201d) was collected. Photosynthetic efficiency Fv/Fm:\u00a0 Samples (2 mL) were taken from each bottle at regular intervals (see above) during the light treatment exposure and subsequent recovery period; samples were then dark-incubated at 15\u00b0C for 20 min. After dark acclimation, Fv/Fm was measured using a Walz Water-PAM pulse amplitude-modulated fluorometer.
attribute	NC_GLOBAL	awards_0_award_nid	String	614837
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1434842
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1434842
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Irradiance exposure experiments S. Strom, PI Version 22 January 2018
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2018-01-11T20:04:43Z
attribute	NC_GLOBAL	date_modified	String	2019-03-18T18:42:30Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.723266.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/723266
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Fluorometer
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used to measure FvFm
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	723341
attribute	NC_GLOBAL	instruments_0_description	String	A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/113/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Fluorometer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	484
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Walz Water-PAM pulse amplitude-modulated fluorometer
attribute	NC_GLOBAL	instruments_1_acronym	String	LI-COR LI-1000
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Used to collect PAR data
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	723340
attribute	NC_GLOBAL	instruments_1_description	String	"The LI-1000 DataLogger is a 10 channel datalogger that functions both as a data logging device and a multichannel, autoranging meter. The electronics of the LI-1000 have been optimized for highly accurate measurement of LI-COR radiation sensors which have a current signal" (from LI-COR Datalogging Instruction Manual, p 1). LI-COR began manufacturing these instruments in 1985 and discontinued in 1998. Serial Numbers for this model have the prefix of LDL-XXXX. (www.licor.com)
attribute	NC_GLOBAL	instruments_1_instrument_name	String	LI-COR LI-1000 Data Logger
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	543
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Li-Cor LI-1400 data logger with a 2π sensor
attribute	NC_GLOBAL	keywords	String	available, bco, bco-dmo, biological, chemical, data, dataset, date, dinoflagellate, Dinoflagellate_species, dmo, erddap, experiment, Experiment_Date, Experiment_ID, FvFm, iso, management, max, Max_PAR, number, oceanography, office, par, photosynthetically, preliminary, radiation, sample, Sample_Number, sampling, Sampling_Time, species, time, total, Total_PAR, treatment
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/723266/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/723266
attribute	NC_GLOBAL	param_mapping	String	{'723266': {'ISO_DateTime_UTC': 'flag - time'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/723266/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Western Washington University - Shannon Point Marine Center
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	SPMC
attribute	NC_GLOBAL	people_0_person_name	String	Suzanne Strom
attribute	NC_GLOBAL	people_0_person_nid	String	50471
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Hannah Ake
attribute	NC_GLOBAL	people_1_person_nid	String	650173
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	Protist signaling
attribute	NC_GLOBAL	projects_0_acronym	String	Protist signaling
attribute	NC_GLOBAL	projects_0_description	String	Description from NSF proposal: This proposal arises from the central premise that the oxidative stress response is an emergent property of phototrophic cellular systems, with implications for nearly every aspect of a phytoplankton cell’s life in the upper ocean. Oxidative stress (OS) arises from the uncompensated production of reactive oxygen species (ROS) within a cell, which can occur in response to a myriad of environmental stressors (e.g. nutrient limitation, temperature extremes, toxins, variable light exposure). In addition to the biochemical damage and physiological impairment that OS can cause, the phytoplankton OS response also includes increased net production and extracellular release of ROS, osmolytes, and other compounds that are known or suspected to be potent signals regulating protist behavior. We hypothesize that, through chemical signaling, oxidative stress acts to govern relationships among environmental variability, phytoplankton condition, and protist predation. Our proposed study of these integrated signaling and response processes has three overarching objectives: 1) Create and characterize oxidatively stressed phytoplankton. We will use light stress (variable exposure to visible light and UV) to create oxidatively stressed phytoplankton in the laboratory. Common coastal taxa with contrasting stress responses will be characterized using an array of fluorescent probes, biochemical measurements, and physiological assays. In addition, intracellular production and extracellular release of ROS and the associated chemical signal DMSP will be quantified. Use of Phaeodactylum tricornutum light stress mutants will add an independent means of connecting OS to signal production and predation response. 2) Examine protist predator responses to oxidatively stressed phytoplankton and associated chemical signals. Responses will be investigated by means of manipulation experiments and thorough characterization of associated signal chemistry. Assessment of predator response will be via predation rate measurements and population aggregation/dispersal behaviors in structured columns. 3) Investigate the prevalence of OS, its environmental correlates, and the microzooplankton predation response in the natural waters of a well-characterized local embayment. Application of ROS probes and OS assays to the natural environment and the design of OS manipulation experiments will be informed by the laboratory experiments using local protist species. Our work will help to elucidate some of the multiple ways in which the OS response can affect phytoplankton fitness, contributing information that can be used to characterize the position of key coastal species along an OS response spectrum. Ultimately such information could be used in trait-based conceptual and numerical models in a manner analogous to cell size and other 'master traits'. Our research will also inform the relatively new and exciting field of chemical signaling in planktonic communities, exploring DMSP- and ROS-based signaling between two of the most significant groups in the plankton, the eukaryotic phytoplankton and their protist predators. Finally, findings will help elucidate the links between environmental stress, phytoplankton response, and predation in planktonic ecosystems. These links relate to central issues in biological oceanography, including the predator-prey interactions that influence bloom demise, and the mechanisms by which protists feed selectively and thereby structure prey communities. The proposed research is a cross-cutting endeavor that unites subjects usually studied in isolation through a novel conceptual framework. Thus the findings have the potential to generate broadly applicable new insights into the ecological and evolutionary regulation of this key trophic link in planktonic food webs.
attribute	NC_GLOBAL	projects_0_end_date	String	2017-08
attribute	NC_GLOBAL	projects_0_geolocation	String	Salish Sea: 48.5, -122.75
attribute	NC_GLOBAL	projects_0_name	String	Environmental stress and signaling based on reactive oxygen species among planktonic protists
attribute	NC_GLOBAL	projects_0_project_nid	String	614838
attribute	NC_GLOBAL	projects_0_start_date	String	2014-09
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Photophysiological responses of two dinoflagellate species used in natural high light exposure experiments (Protist Signaling project)
attribute	NC_GLOBAL	time_coverage_end	String	2015-09-10T19:39:00Z
attribute	NC_GLOBAL	time_coverage_start	String	2015-07-15T12:09:00Z
attribute	NC_GLOBAL	title	String	Photophysiological responses of two dinoflagellate species used in natural high light exposure experiments (Protist Signaling project)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Experiment_ID		String
attribute	Experiment_ID	bcodmo_name	String	exp_id
attribute	Experiment_ID	description	String	Experiment ID number
attribute	Experiment_ID	long_name	String	Experiment ID
attribute	Experiment_ID	units	String	unitless
variable	Experiment_Date		String
attribute	Experiment_Date	bcodmo_name	String	date
attribute	Experiment_Date	description	String	Experiment date; YYYY/MM/DD
attribute	Experiment_Date	long_name	String	Experiment Date
attribute	Experiment_Date	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	Experiment_Date	time_precision	String	1970-01-01
attribute	Experiment_Date	units	String	unitless
variable	Dinoflagellate_species		String
attribute	Dinoflagellate_species	bcodmo_name	String	species
attribute	Dinoflagellate_species	description	String	Species of sample
attribute	Dinoflagellate_species	long_name	String	Dinoflagellate Species
attribute	Dinoflagellate_species	units	String	unitless
variable	Max_PAR		float
attribute	Max_PAR	_FillValue	float	NaN
attribute	Max_PAR	actual_range	float	0.7845, 1607.0
attribute	Max_PAR	bcodmo_name	String	PAR
attribute	Max_PAR	description	String	Maximum instantaneous PAR received during light exposure treatment
attribute	Max_PAR	long_name	String	Max PAR
attribute	Max_PAR	units	String	umol photons m^-2 s^-1
variable	Total_PAR		float
attribute	Total_PAR	_FillValue	float	NaN
attribute	Total_PAR	actual_range	float	0.004833, 9.897
attribute	Total_PAR	bcodmo_name	String	PAR
attribute	Total_PAR	description	String	Total dose of photosynthetically active radiation received during lightexposure treatment
attribute	Total_PAR	long_name	String	Total PAR
attribute	Total_PAR	units	String	mol photons m^-2
variable	Sampling_Time		String
attribute	Sampling_Time	bcodmo_name	String	time
attribute	Sampling_Time	description	String	Time of sampling; HH:MM
attribute	Sampling_Time	long_name	String	Sampling Time
attribute	Sampling_Time	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/AHMSAA01/
attribute	Sampling_Time	units	String	unitless
variable	Treatment		String
attribute	Treatment	bcodmo_name	String	treatment
attribute	Treatment	description	String	Treatment
attribute	Treatment	long_name	String	Treatment
attribute	Treatment	units	String	unitless
variable	Sample_Number		byte
attribute	Sample_Number	_FillValue	byte	127
attribute	Sample_Number	actual_range	byte	1, 4
attribute	Sample_Number	bcodmo_name	String	sample
attribute	Sample_Number	colorBarMaximum	double	100.0
attribute	Sample_Number	colorBarMinimum	double	0.0
attribute	Sample_Number	description	String	Sample ID Number
attribute	Sample_Number	long_name	String	Sample Number
attribute	Sample_Number	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	Sample_Number	units	String	unitless
variable	FvFm		float
attribute	FvFm	_FillValue	float	NaN
attribute	FvFm	actual_range	float	0.0, 0.76
attribute	FvFm	bcodmo_name	String	Fv2Fm
attribute	FvFm	description	String	Photosynthetic efficiency (variable fluorescence normalized to maximum fluorescence)
attribute	FvFm	long_name	String	FV FM
attribute	FvFm	units	String	unitless
variable	time		double
attribute	time	_CoordinateAxisType	String	Time
attribute	time	actual_range	double	1.43696214E9, 1.44191394E9
attribute	time	axis	String	T
attribute	time	bcodmo_name	String	ISO_DateTime_UTC
attribute	time	description	String	DateTime ISO Formatted; UTC
attribute	time	ioos_category	String	Time
attribute	time	long_name	String	ISO Date Time UTC
attribute	time	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/
attribute	time	source_name	String	ISO_DateTime_UTC
attribute	time	standard_name	String	time
attribute	time	time_origin	String	01-JAN-1970 00:00:00
attribute	time	time_precision	String	1970-01-01T00:00:00Z
attribute	time	units	String	seconds since 1970-01-01T00:00:00Z

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.