ERDDAP > info > bcodmo_dataset_723277

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Dinoflagellate culturing] - Size and chemical responses of two dinoflagellate species used in natural high light exposure experiments (Protist Signaling project) (Environmental stress and signaling based on reactive oxygen species among planktonic protists)		I   M	background			BCO-DMO	bcodmo_dataset_723277

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Dinoflagellate culturing:\u00a0Alexandrium fundyense (strain CCMP 1911) was obtained from the National Center for Marine Algae and Microbiota (NCMA). The strain was originally isolated from Sequim Bay, Washington, USA. Heterocapsa rotundata (strain K-0483), obtained from the Scandanavian Culture Collection of Algae and Protozoa, was isolated from the southern Kattegat Sea near Denmark. Dinoflagellate cultures were maintained in f/2 medium without added silicate at 15\u00b0C under a 12L:12D light cycle and transferred every two to three weeks into new media. Growth irradiance for A. fundyense was 53 \u00b5mol photons m-2 s-1; growth irradiance for H. rotundata was 12 \u00b5mol photons m-2 s-1. Cell concentration and cell volume: Cell concentration (cells mL-1) and cell volume (\u00b5m3 cell-1) estimates for H. rotundata were obtained from live samples measured with a Beckman Coulter Z2 Particle Count and Size Analyzer with Z2 AccuComp software. For A. fundyense, samples were preserved in acid Lugol\u2019s solution (final concentration 2%). Cells were counted in a 1 ml Sedgewick Rafter chamber using light microscopy; cell volume was estimated from length and width of cells (n = 42 or 83 cells per sample) measured with Leica Application Suite X image analysis software and assuming cell shape was approximated by an oblate ellipsoid. Chlorophyll-a: Chl-a concentrations were measured by filtering samples through 0.7 \u00b5m effective pore size 25 mm glass fiber filters. Pigments were then extracted in 90% acetone for 24 h (dark, -20\u00b0C) and fluorescence was measured on a Turner 10-AU fluorometer using the acidification method. Concentrations are reported per cell and per unit cell volume. Dissolved and particulate dimethylsulfoniopropionate (DMSP):\u00a0 DMSP samples (4 ml) were gravity-filtered through precombusted 0.7 \u00b5m effective pore size 25 mm glass fiber filters so as not to rupture the cells. For measurement of particulate (intracellular) DMSP (DMSPp), filters were placed into sealed 20-ml glass vials containing 3 ml of 5 N NaOH. For measurement of DMSP in the dissolved (extracellular) phase (DMSPd), the first 4.5 mL of each sample\u2019s filtrate were caught in a 5 ml polystyrene culture tube, which was capped and stored at -80\u00b0C. Later, DMSPd samples were thawed and sparged with N2 gas for 1 min to remove any dimethyl sulfide (DMS) already present. Each sparged sample (4 ml) was then dispensed into a 20-ml vial containing 1 ml of 5 N NaOH, and sealed. Upon being sealed, prepared vials for either analysis were allowed to equilibrate for at least 24 h, allowing for the 1:1 conversion of DMSP to gaseous DMS, detectable via gas chromatography. Standards for DMSPp were prepared from pre-diluted DMSP solutions at the same time that samples were filtered and sealed into vials, while DMSPd standards were made at the same time that samples were sparged and sealed into vials. Samples and standards were analyzed using a Shimadzu Gas Chromatograph 14-A equipped with a flame photometric detector and a Supelco packed Chromosil 330 column. The chromatograph was operated isothermally at 90\u00b0C with flow rates of hydrogen, air, and helium (carrier gas) at 50, 60, and 150 kPa, respectively. DMSPp samples and standards were measured via direct injection while DMSPd samples and standards were measured with a headspace sweep (He flow rate 40 kPa). DMSPp concentrations are reported per cell and per unit cell volume. DMSPd concentrations are reported per cell and per unit seawater volume.
attribute	NC_GLOBAL	awards_0_award_nid	String	614837
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1434842
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1434842
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Dinoflagellate culturing S. Strom, PI Version 11 January 2018
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2018-01-11T20:06:39Z
attribute	NC_GLOBAL	date_modified	String	2019-03-15T20:12:34Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.723277.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/723277
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Gas Chromatograph
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used to analyze samples and standards
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	723350
attribute	NC_GLOBAL	instruments_0_description	String	Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB02/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Gas Chromatograph
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	661
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Shimadzu Gas Chromatograph
attribute	NC_GLOBAL	instruments_1_acronym	String	ACC
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Used to determine cell concentration
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	723351
attribute	NC_GLOBAL	instruments_1_description	String	Automated Cell Counter (ACC) - a tool used for counting live and/or dead cells in a culture.  It can also be used to size particles.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Automated Cell Counter
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	651528
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Beckman Coulter Z2 Particle Count and Size Analyzer
attribute	NC_GLOBAL	keywords	String	available, bco, bco-dmo, biological, cell, cellvol1, chemical, chemistry, chlorophyll, concentration, concentration_of_chlorophyll_in_sea_water, counts, data, dataset, date, defense, determinations, dinoflagellate, Dinoflagellate_species, dissolved, dmo, dmsp, during, earth, Earth Science > Oceans > Ocean Chemistry > Chlorophyll, erddap, experiment, Experiment_ID, exposure, fmolcell1, management, meteorological, mmol, nmol, ocean, oceanography, oceans, office, par, particulate, photosynthetically, preliminary, program, radiation, replicate, Replicate_cell_counts, Replicate_cell_volume, Replicate_determinations_chlorophyll_a, Replicate_determinations_chlorophyll_a_per_unit_cell_volume, Replicate_determinations_dissolved_DMSP_fmolcell1, Replicate_determinations_dissolved_DMSP_nmolL1, Replicate_determinations_particulate_DMSP_fmolcell1, Replicate_determinations_particulate_DMSP_mmolL_cellvol1, Replicate_determinations_total_DMSP, sampling, Sampling_timepoint, satellite, science, sea, seawater, species, time, timepoint, total, Total_PAR_during_exposure_treatment, treatment, volume, water
attribute	NC_GLOBAL	keywords_vocabulary	String	GCMD Science Keywords
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/723277/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/723277
attribute	NC_GLOBAL	param_mapping	String	{'723277': {'Experiment_Date': 'flag - time'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/723277/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Western Washington University - Shannon Point Marine Center
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	SPMC
attribute	NC_GLOBAL	people_0_person_name	String	Suzanne Strom
attribute	NC_GLOBAL	people_0_person_nid	String	50471
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Hannah Ake
attribute	NC_GLOBAL	people_1_person_nid	String	650173
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	Protist signaling
attribute	NC_GLOBAL	projects_0_acronym	String	Protist signaling
attribute	NC_GLOBAL	projects_0_description	String	Description from NSF proposal: This proposal arises from the central premise that the oxidative stress response is an emergent property of phototrophic cellular systems, with implications for nearly every aspect of a phytoplankton cell’s life in the upper ocean. Oxidative stress (OS) arises from the uncompensated production of reactive oxygen species (ROS) within a cell, which can occur in response to a myriad of environmental stressors (e.g. nutrient limitation, temperature extremes, toxins, variable light exposure). In addition to the biochemical damage and physiological impairment that OS can cause, the phytoplankton OS response also includes increased net production and extracellular release of ROS, osmolytes, and other compounds that are known or suspected to be potent signals regulating protist behavior. We hypothesize that, through chemical signaling, oxidative stress acts to govern relationships among environmental variability, phytoplankton condition, and protist predation. Our proposed study of these integrated signaling and response processes has three overarching objectives: 1) Create and characterize oxidatively stressed phytoplankton. We will use light stress (variable exposure to visible light and UV) to create oxidatively stressed phytoplankton in the laboratory. Common coastal taxa with contrasting stress responses will be characterized using an array of fluorescent probes, biochemical measurements, and physiological assays. In addition, intracellular production and extracellular release of ROS and the associated chemical signal DMSP will be quantified. Use of Phaeodactylum tricornutum light stress mutants will add an independent means of connecting OS to signal production and predation response. 2) Examine protist predator responses to oxidatively stressed phytoplankton and associated chemical signals. Responses will be investigated by means of manipulation experiments and thorough characterization of associated signal chemistry. Assessment of predator response will be via predation rate measurements and population aggregation/dispersal behaviors in structured columns. 3) Investigate the prevalence of OS, its environmental correlates, and the microzooplankton predation response in the natural waters of a well-characterized local embayment. Application of ROS probes and OS assays to the natural environment and the design of OS manipulation experiments will be informed by the laboratory experiments using local protist species. Our work will help to elucidate some of the multiple ways in which the OS response can affect phytoplankton fitness, contributing information that can be used to characterize the position of key coastal species along an OS response spectrum. Ultimately such information could be used in trait-based conceptual and numerical models in a manner analogous to cell size and other 'master traits'. Our research will also inform the relatively new and exciting field of chemical signaling in planktonic communities, exploring DMSP- and ROS-based signaling between two of the most significant groups in the plankton, the eukaryotic phytoplankton and their protist predators. Finally, findings will help elucidate the links between environmental stress, phytoplankton response, and predation in planktonic ecosystems. These links relate to central issues in biological oceanography, including the predator-prey interactions that influence bloom demise, and the mechanisms by which protists feed selectively and thereby structure prey communities. The proposed research is a cross-cutting endeavor that unites subjects usually studied in isolation through a novel conceptual framework. Thus the findings have the potential to generate broadly applicable new insights into the ecological and evolutionary regulation of this key trophic link in planktonic food webs.
attribute	NC_GLOBAL	projects_0_end_date	String	2017-08
attribute	NC_GLOBAL	projects_0_geolocation	String	Salish Sea: 48.5, -122.75
attribute	NC_GLOBAL	projects_0_name	String	Environmental stress and signaling based on reactive oxygen species among planktonic protists
attribute	NC_GLOBAL	projects_0_project_nid	String	614838
attribute	NC_GLOBAL	projects_0_start_date	String	2014-09
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Size and chemical responses of two dinoflagellate species used in natural high light exposure experiments (Protist Signaling project)
attribute	NC_GLOBAL	time_coverage_end	String	2015-09-10
attribute	NC_GLOBAL	time_coverage_start	String	2015-07-15
attribute	NC_GLOBAL	title	String	[Dinoflagellate culturing] - Size and chemical responses of two dinoflagellate species used in natural high light exposure experiments (Protist Signaling project) (Environmental stress and signaling based on reactive oxygen species among planktonic protists)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Experiment_ID		String
attribute	Experiment_ID	bcodmo_name	String	exp_id
attribute	Experiment_ID	description	String	Experiment ID number
attribute	Experiment_ID	long_name	String	Experiment ID
attribute	Experiment_ID	units	String	unitless
variable	time		double
attribute	time	_CoordinateAxisType	String	Time
attribute	time	actual_range	double	1.4369184E9, 1.4418432E9
attribute	time	axis	String	T
attribute	time	bcodmo_name	String	date
attribute	time	description	String	Experiment date; YYYY/MM/DD
attribute	time	ioos_category	String	Time
attribute	time	long_name	String	Experiment Date
attribute	time	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	time	source_name	String	Experiment_Date
attribute	time	standard_name	String	time
attribute	time	time_origin	String	01-JAN-1970 00:00:00
attribute	time	time_precision	String	1970-01-01
attribute	time	units	String	seconds since 1970-01-01T00:00:00Z
variable	Dinoflagellate_species		String
attribute	Dinoflagellate_species	bcodmo_name	String	species
attribute	Dinoflagellate_species	description	String	Species of sample
attribute	Dinoflagellate_species	long_name	String	Dinoflagellate Species
attribute	Dinoflagellate_species	units	String	unitless
variable	Total_PAR_during_exposure_treatment		float
attribute	Total_PAR_during_exposure_treatment	_FillValue	float	NaN
attribute	Total_PAR_during_exposure_treatment	actual_range	float	0.004, 9.897
attribute	Total_PAR_during_exposure_treatment	bcodmo_name	String	PAR
attribute	Total_PAR_during_exposure_treatment	description	String	Total dose of photosynthetically active radiation received during light exposure treatment
attribute	Total_PAR_during_exposure_treatment	long_name	String	Total PAR During Exposure Treatment
attribute	Total_PAR_during_exposure_treatment	units	String	mol photons m^-2
variable	Sampling_timepoint		String
attribute	Sampling_timepoint	bcodmo_name	String	unknown
attribute	Sampling_timepoint	description	String	Time sample was taken
attribute	Sampling_timepoint	long_name	String	Sampling Timepoint
attribute	Sampling_timepoint	units	String	unitless
variable	Replicate_cell_counts		int
attribute	Replicate_cell_counts	_FillValue	int	2147483647
attribute	Replicate_cell_counts	actual_range	int	1603, 34083
attribute	Replicate_cell_counts	bcodmo_name	String	cell_concentration
attribute	Replicate_cell_counts	description	String	Cell concentration in seawater media
attribute	Replicate_cell_counts	long_name	String	Replicate Cell Counts
attribute	Replicate_cell_counts	units	String	cells ml-1
variable	Replicate_cell_volume		float
attribute	Replicate_cell_volume	_FillValue	float	NaN
attribute	Replicate_cell_volume	actual_range	float	119.98, 23641.0
attribute	Replicate_cell_volume	bcodmo_name	String	volume
attribute	Replicate_cell_volume	description	String	Volume of individual dinoflagellate cells
attribute	Replicate_cell_volume	long_name	String	Replicate Cell Volume
attribute	Replicate_cell_volume	units	String	um^3
variable	Replicate_determinations_chlorophyll_a		float
attribute	Replicate_determinations_chlorophyll_a	_FillValue	float	NaN
attribute	Replicate_determinations_chlorophyll_a	actual_range	float	0.22, 42.73
attribute	Replicate_determinations_chlorophyll_a	bcodmo_name	String	chlorophyll a
attribute	Replicate_determinations_chlorophyll_a	colorBarMaximum	double	30.0
attribute	Replicate_determinations_chlorophyll_a	colorBarMinimum	double	0.03
attribute	Replicate_determinations_chlorophyll_a	colorBarScale	String	Log
attribute	Replicate_determinations_chlorophyll_a	description	String	Chlorophyll-a content of cells
attribute	Replicate_determinations_chlorophyll_a	long_name	String	Concentration Of Chlorophyll In Sea Water
attribute	Replicate_determinations_chlorophyll_a	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/
attribute	Replicate_determinations_chlorophyll_a	units	String	pg cell-1
variable	Replicate_determinations_chlorophyll_a_per_unit_cell_volume		float
attribute	Replicate_determinations_chlorophyll_a_per_unit_cell_volume	_FillValue	float	NaN
attribute	Replicate_determinations_chlorophyll_a_per_unit_cell_volume	actual_range	float	1314.94, 8746.33
attribute	Replicate_determinations_chlorophyll_a_per_unit_cell_volume	bcodmo_name	String	chlorophyll a
attribute	Replicate_determinations_chlorophyll_a_per_unit_cell_volume	colorBarMaximum	double	30.0
attribute	Replicate_determinations_chlorophyll_a_per_unit_cell_volume	colorBarMinimum	double	0.03
attribute	Replicate_determinations_chlorophyll_a_per_unit_cell_volume	colorBarScale	String	Log
attribute	Replicate_determinations_chlorophyll_a_per_unit_cell_volume	description	String	Intracellular concentration of chlorophyll-a
attribute	Replicate_determinations_chlorophyll_a_per_unit_cell_volume	long_name	String	Concentration Of Chlorophyll In Sea Water
attribute	Replicate_determinations_chlorophyll_a_per_unit_cell_volume	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/
attribute	Replicate_determinations_chlorophyll_a_per_unit_cell_volume	units	String	mg L-1
variable	Replicate_determinations_particulate_DMSP_fmolcell1		float
attribute	Replicate_determinations_particulate_DMSP_fmolcell1	_FillValue	float	NaN
attribute	Replicate_determinations_particulate_DMSP_fmolcell1	actual_range	float	10.52, 3986.6
attribute	Replicate_determinations_particulate_DMSP_fmolcell1	bcodmo_name	String	dimethylsulphoniopropionate
attribute	Replicate_determinations_particulate_DMSP_fmolcell1	description	String	Dimethylsulfoniopropionate content of cells
attribute	Replicate_determinations_particulate_DMSP_fmolcell1	long_name	String	Replicate Determinations Particulate DMSP Fmolcell1
attribute	Replicate_determinations_particulate_DMSP_fmolcell1	units	String	fmol cell-1
variable	Replicate_determinations_particulate_DMSP_mmolL_cellvol1		float
attribute	Replicate_determinations_particulate_DMSP_mmolL_cellvol1	_FillValue	float	NaN
attribute	Replicate_determinations_particulate_DMSP_mmolL_cellvol1	actual_range	float	71.34, 199.38
attribute	Replicate_determinations_particulate_DMSP_mmolL_cellvol1	bcodmo_name	String	dimethylsulphoniopropionate
attribute	Replicate_determinations_particulate_DMSP_mmolL_cellvol1	description	String	Intracellular concentration of dimethylsulfoniopropionate
attribute	Replicate_determinations_particulate_DMSP_mmolL_cellvol1	long_name	String	Replicate Determinations Particulate DMSP Mmol L Cellvol1
attribute	Replicate_determinations_particulate_DMSP_mmolL_cellvol1	units	String	mmol L cell vol-1
variable	Replicate_determinations_dissolved_DMSP_nmolL1		float
attribute	Replicate_determinations_dissolved_DMSP_nmolL1	_FillValue	float	NaN
attribute	Replicate_determinations_dissolved_DMSP_nmolL1	actual_range	float	7.11, 1037.85
attribute	Replicate_determinations_dissolved_DMSP_nmolL1	bcodmo_name	String	dimethylsulphoniopropionate
attribute	Replicate_determinations_dissolved_DMSP_nmolL1	description	String	Concentration of dimethylsulfoniopropionate dissolved in seawater medium normalized to cell concentration in medium
attribute	Replicate_determinations_dissolved_DMSP_nmolL1	long_name	String	Replicate Determinations Dissolved DMSP Nmol L1
attribute	Replicate_determinations_dissolved_DMSP_nmolL1	units	String	nmol L-1
variable	Replicate_determinations_dissolved_DMSP_fmolcell1		float
attribute	Replicate_determinations_dissolved_DMSP_fmolcell1	_FillValue	float	NaN
attribute	Replicate_determinations_dissolved_DMSP_fmolcell1	actual_range	float	0.27, 601.65
attribute	Replicate_determinations_dissolved_DMSP_fmolcell1	bcodmo_name	String	dimethylsulphoniopropionate
attribute	Replicate_determinations_dissolved_DMSP_fmolcell1	description	String	Concentration of dimethylsulfoniopropionate dissolved in seawater medium normalized to cell concentration in medium
attribute	Replicate_determinations_dissolved_DMSP_fmolcell1	long_name	String	Replicate Determinations Dissolved DMSP Fmolcell1
attribute	Replicate_determinations_dissolved_DMSP_fmolcell1	units	String	fmol cell-1
variable	Replicate_determinations_total_DMSP		float
attribute	Replicate_determinations_total_DMSP	_FillValue	float	NaN
attribute	Replicate_determinations_total_DMSP	actual_range	float	0.01, 9.28
attribute	Replicate_determinations_total_DMSP	bcodmo_name	String	dimethylsulphoniopropionate
attribute	Replicate_determinations_total_DMSP	description	String	Concentration of dissolved plus particulate dimethylsulfoniopropionate
attribute	Replicate_determinations_total_DMSP	long_name	String	Replicate Determinations Total DMSP
attribute	Replicate_determinations_total_DMSP	units	String	umol L-1

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.