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Title Sum-
Institution Dataset ID
   set  data   graph     files  public Potential denitrification and N2 fixation from slurry assays from Little Lagoon, Alabama
collected from 2012-2013
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Little Lagoon is a shallow coastal lagoon that is tidally connected to the
Gulf of Mexico but has no riverine inputs. The water in the lagoon is
replenished solely from precipitation and groundwater inputs primarily on the
East end (Su et al. 2012). Because of the rapid development in Baldwin County,
a large amount of NO3- enters the Little Lagoon system through SGD (Murgulet &
Tick 2008). In this region, there can be rapid changes in the depth to
groundwater (Fig. 4.1 inset) and episodic SGD inputs to the lagoon (Su et
al.2013). Within the lagoon, three sites were selected (East, Mouth, and West)
to represent the gradient that exists across the lagoon from the input of
groundwater. Sites were sampled on a near-monthly basis from February 2012 to
February 2013.

Potential denitrification and N2 fixation from slurry assays

The top 50 mm of sediment at each site were collected in duplicate with a
large core (95 mm ID) and homogenized. Potential denitrification rates were
measured following the acetylene inhibition technique (S\u00f8rensen 1978) but
may underestimate denitrification supported by coupled nitrification-
denitrification, as this method inhibits nitrification. To triplicate serum
vials, approximately 20 g of sediments and filtered (0.7 micron) site water
were added at various treatments (control and N addition (100 \u03bcM and 500
\u03bcM KNO3 -). Samples were sealed with a butyl rubber stopper, capped and
flushed with N2 gas for 10 minutes. After the addition of C2H2 (10% v/v) and a
1-hour incubation, headspace gas samples were injected into evacuated 12 ml
Exetainer vials and N2O production was quantified with a Shimadzu GC-2014 with
an electron capture detector (GC-ECD) within 24 hours.

Potential N2 fixation rates were measured as ethylene (C2H4) production from
acetylene (C2H2) reduction (Welsh et al. 1996) in triplicate from slurry
assays containing 20 g of homogenized sediment and filtered (0.7 micron) site
water. Rates of N2 fixation by sulfate reducing bacteria (SRB) were determined
after the addition of sodium molybdate as a specific inhibitor of the sulfate
reduction process (Hardy et al. 1973, Capone 1993). After C2H4 analysis on a
Shimadzu gas chromatograph (GC-2014) with flame ionization detection (GC-FID),
production rates of C2H4 were converted to potential N2 fixation rates using a
C2H2:N2 reduction ratio of 3:1 (Capone 1993).

Additional methodology can be found in:

Bernard, Rebecca & Mortazavi, Behzad & A. Kleinhuizen, Alice. (2015).
Dissimilatory nitrate reduction to ammonium (DNRA) seasonally dominates
NO3\u2212 reduction pathways in an anthropogenically impacted sub-tropical
coastal lagoon. Biogeochemistry. 125.
attribute NC_GLOBAL awards_0_award_nid String 497637
attribute NC_GLOBAL awards_0_award_number String OCE-0962008
attribute NC_GLOBAL awards_0_data_url String http://nsf.gov/awardsearch/showAward?AWD_ID=0962008 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String DNF and N2 Data
B. Mortazavi and W. Burnett, PIs
Version 16 January 2018
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2018-01-17T20:11:20Z
attribute NC_GLOBAL date_modified String 2019-03-15T18:09:49Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.723957.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/723957 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, chemical, data, dataset, date, description, dmo, east, East_SE, erddap, management, mouth, Mouth_SE, oceanography, office, preliminary, value, Value_Description, west, West_SE, year
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/723957/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/723957 (external link)
attribute NC_GLOBAL param_mapping String {'723957': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/723957/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String National Science Foundation
attribute NC_GLOBAL people_0_affiliation_acronym String NSF-DEB
attribute NC_GLOBAL people_0_person_name String Dr Behzad Mortazavi
attribute NC_GLOBAL people_0_person_nid String 491316
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Florida State University
attribute NC_GLOBAL people_1_affiliation_acronym String FSU - EOAS
attribute NC_GLOBAL people_1_person_name String Dr William C. Burnett
attribute NC_GLOBAL people_1_person_nid String 491315
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String National Science Foundation
attribute NC_GLOBAL people_2_affiliation_acronym String NSF-DEB
attribute NC_GLOBAL people_2_person_name String Dr Behzad Mortazavi
attribute NC_GLOBAL people_2_person_nid String 491316
attribute NC_GLOBAL people_2_role String Contact
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Hannah Ake
attribute NC_GLOBAL people_3_person_nid String 650173
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String LittleLagoonGroundwater
attribute NC_GLOBAL projects_0_acronym String LittleLagoonGroundwater
attribute NC_GLOBAL projects_0_description String
This project�investigated the link between submarine groundwater discharge (SGD) and microalgal dynamics in Little Lagoon, Alabama. �In contrast to most near-shore environments, it is fully accessible; has no riverine inputs; and is large enough to display ecological diversity (c. 14x 0.75 km) yet small enough to be comprehensively sampled on appropriate temporal and spatial scales. The PIs have previously demonstrated that the lagoon is a hot-spot for toxic blooms of the diatom Pseudo-nitzchia spp. that are correlated with discharge from the surficial aquifer. This project�assessed variability in SGD, the dependence of benthic nutrient fluxes on microphytobenthos (MPB) abundance and productivity, and the response of the phytoplankton to nutrient enrichment and dilution. The work integrated multiple temporal and spatial scales and demonstrated both the relative importance of SGD vs. benthic recycling as a source of nutrients, and the role of SGD in structuring the microalgal community. (paraphrased from Award abstract)
attribute NC_GLOBAL projects_0_end_date String 2014-08
attribute NC_GLOBAL projects_0_geolocation String southern Alabama, east of Mobile
attribute NC_GLOBAL projects_0_name String Groundwater Discharge, Benthic Coupling and Microalgal Community Structure in a Shallow Coastal Lagoon
attribute NC_GLOBAL projects_0_project_nid String 491318
attribute NC_GLOBAL projects_0_start_date String 2010-03
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String Year
attribute NC_GLOBAL summary String Potential denitrification and N2 fixation from slurry assays from Little Lagoon, Alabama collected from 2012-2013
attribute NC_GLOBAL title String Potential denitrification and N2 fixation from slurry assays from Little Lagoon, Alabama collected from 2012-2013
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Year   byte  
attribute Year _FillValue byte 127
attribute Year actual_range byte 1, 1
attribute Year bcodmo_name String unknown
attribute Year description String Year ID that samples were taken
attribute Year long_name String Year
attribute Year units String unitless
variable Date   String  
attribute Date bcodmo_name String date
attribute Date description String Month and day that samples were taken; MMM-DD
attribute Date long_name String Date
attribute Date nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute Date units String unitless
variable Value_Description   String  
attribute Value_Description bcodmo_name String unknown
attribute Value_Description description String Description of the measurment taken; description includes relevant units for each sample taken
attribute Value_Description long_name String Value Description
attribute Value_Description units String unitless
variable Mouth   float  
attribute Mouth _FillValue float NaN
attribute Mouth actual_range float 1.19, 13.8
attribute Mouth bcodmo_name String N
attribute Mouth description String Denitrification and Nitrogen fixation values collected at the site Mouth; location of site is 30.243683, -87.738407
attribute Mouth long_name String Mouth
attribute Mouth units String nanomol of nitrogen per gram per hour
variable Mouth_SE   float  
attribute Mouth_SE _FillValue float NaN
attribute Mouth_SE actual_range float 0.05, 1.59
attribute Mouth_SE bcodmo_name String N
attribute Mouth_SE description String Standard error of the values collected at the site Mouth
attribute Mouth_SE long_name String Mouth SE
attribute Mouth_SE units String nanomol of nitrogen per gram per hour
variable West   float  
attribute West _FillValue float NaN
attribute West actual_range float 0.98, 14.72
attribute West bcodmo_name String N
attribute West description String Denitrification and Nitrogen fixation values collected at the site West; location of site is 30.247181, -87.767856
attribute West long_name String West
attribute West units String nanomol of nitrogen per gram per hour
variable West_SE   float  
attribute West_SE _FillValue float NaN
attribute West_SE actual_range float 0.02, 0.74
attribute West_SE bcodmo_name String N
attribute West_SE description String Standard error of the values collected at the site West
attribute West_SE long_name String West SE
attribute West_SE units String nanomol of nitrogen per gram per hour
variable East   float  
attribute East _FillValue float NaN
attribute East actual_range float 0.58, 14.56
attribute East bcodmo_name String N
attribute East description String Denitrification and Nitrogen fixation values collected at the site East; location of site is 30.253347, -87.724729
attribute East long_name String East
attribute East units String nanomol of nitrogen per gram per hour
variable East_SE   float  
attribute East_SE _FillValue float NaN
attribute East_SE actual_range float 0.02, 2.94
attribute East_SE bcodmo_name String N
attribute East_SE description String Standard error of the values collected at the site East
attribute East_SE long_name String East SE
attribute East_SE units String nanomol of nitrogen per gram per hour

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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