ERDDAP > info > bcodmo_dataset_723966

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Denitrification and DNRA] - Denitrification and DNRA data from Little Lagoon, Alabama collected from 2012-2013 (Groundwater Discharge, Benthic Coupling and Microalgal Community Structure in a Shallow Coastal Lagoon)		I   M	background			BCO-DMO	bcodmo_dataset_723966

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Little Lagoon is a shallow coastal lagoon that is tidally connected to the Gulf of Mexico but has no riverine inputs. The water in the lagoon is replenished solely from precipitation and groundwater inputs primarily on the East end (Su et al. 2012). Because of the rapid development in Baldwin County, a large amount of NO3- enters the Little Lagoon system through SGD (Murgulet & Tick 2008). In this region, there can be rapid changes in the depth to groundwater (Fig. 4.1 inset) and episodic SGD inputs to the lagoon (Su et al.2013). Within the lagoon, three sites were selected (East, Mouth, and West) to represent the gradient that exists across the lagoon from the input of groundwater. Sites were sampled on a near-monthly basis from February 2012 to February 2013. DNRA\u00a0 Approximately 1 L of outflow water was collected from the inflow water and each core forDNRA analysis. Appropriate sample volume was determined after NH4 + nutrient analysis and expected atom % enrichment. \u03b415N-NH4 + was measured in samples, constructed blanks, and standards that bracketed the NH4 + concentration of the samples following a modified ammonium diffusion procedure (Holmes et al. 1998) that collects NH4 + dissolved in water by converting NH4 + to NH3 under basic conditions and then traps the NH3 on an acidified glass fiber filter. Non diffused standards were prepared according to Stark and Hart (1996) to account for blank corrections. After 15N analysis on a Europa Scientific SL-2020 system (Stable Isotope Lab, Utah State University), DNRA was calculated from the production rate of 15NH4 + (p15NH4 +) during the incubation according to Christensen et. al (2000): (7) where is the production of 15N-NH4 + and D14 and D15 are the denitrification rates of 14N-NO3 - and 15N-NO3 -, respectively. This assumes that DNRA takes place in the same sediment layers as denitrification and that the 15N labeling of NO3 - being reduced to NH4 + equals the 15N labeling of NO3 - being reduced to N2 (Christensen et al. 2000). Denitrification and anammox from slurry assays Volumetric rates of denitrification, anammox, and the relative contribution of anammox to gross N2 production were determined from sediment slurry incubations. Slurry rates for depth-integrated sediments (0-50 mm) were prepared in Exetainers (Thamdrup & Dalsgaard 2002) with artificial seawater (ASW) (70.2g NaCl, 3.0g KCl, 49.4 g MgSO4*7H2O, 5.8g CaCl2*2H2O L-1) constructed at a salinity of 52 and diluted with deionized water to match the salinity of each site. After dilution, homogenized sediment from 0 to 50 mm was added to the ASW and the incubation bottle was sparged with N2 and amended with 100 \u03bcmol L-1 Na15NO3 - (99 atom %). Sediment slurry was dispensed to 12 ml Exetainers, yielding approximately 1 ml of sediment and 11 ml ASW with no headspace. For each site, 12 vials total were incubated with three vials stopped at time points 0 to 36 h. Incubations were stopped by adding 250 \u03bcL of ZnCl2 and resealing the vials without headspace. Denitrification and anammox rates in slurries were calculated according to equations 5 and 6 described below.\u00a0 Excess 29N2 and 30N2 concentrations for intact core and slurry incubations were calculated from dissolved 29N2:28N2 and 30N2:28N2 measured using a MIMS. Rates of excess 29N2 (p29) and 30N2 (p30) production were calculated from the flux calculation described above. Rates of ambient 14N2 production (p14) in core incubations with 15NO3 - tracer addition were determined as (Nielsen 1992, Risgaard-Petersen et al. 2003):\u00a0 (1) p14 = 2 x r14 \u00b1 [p29 + p30 \u00b1 (1 - r16)] The 14N:15N ratio of NO3 - undergoing reduction to N2 (r14) was determined as follows:\u00a0 (2) r14 = [R29 x (1 - ra) - ra] x (2 - ra) ^-1 where R29 was the ratio of p29 to p30 determined for the cores and ra was the relative contribution of anammox to gross N2 production determined in vial slurry incubations. Gross denitrification and anammox rates within intact sediment cores with 15NO3 - tracer addition were calculated as follows:\u00a0 (3) denitrification = p14 \u00b1 (1 - ra) (4) anammox = p14 \u00b1 ra Denitrification stimulated by the added 15N-NO3 - (D15) was calculated from the classical IPT (Nielsen 1992) and these amended rates are a measure of the denitrification capacity under field conditions when NO3 - is not limiting.\u00a0 Rates of denitrification and anammox in vial slurry incubations with 15NO3 - amendments were calculated from the equations of Thamdrup and Dalsgaard (2002): (5) (6) where FN was the fraction of 15N in NO3 -. For months when anammox slurry incubations were not performed (August and November 2012), p14 is calculated as D14 from the IPT (Nielsen 1992). Potential denitrification and anammox rates were converted to an areal basis using the wet weight of the sediment in the slurry. All rates and fluxes pertaining to N species in this study were normalized to one atom N. Additional methodology can be found in: Bernard, Rebecca & Mortazavi, Behzad & A. Kleinhuizen, Alice. (2015). Dissimilatory nitrate reduction to ammonium (DNRA) seasonally dominates NO3\u2212 reduction pathways in an anthropogenically impacted sub-tropical coastal lagoon. Biogeochemistry. 125. 47-64.\u00a0[10.1007/s10533-015-0111-6](\\"https://link.springer.com/article/10.1007%2Fs10533-015-0111-6\\").\u00a0
attribute	NC_GLOBAL	awards_0_award_nid	String	497637
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-0962008
attribute	NC_GLOBAL	awards_0_data_url	String	http://nsf.gov/awardsearch/showAward?AWD_ID=0962008
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Denitrification and DNRA B. Mortazavi and W. Burnett, PIs Version 16 January 2018
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2018-01-17T20:11:39Z
attribute	NC_GLOBAL	date_modified	String	2019-03-15T18:07:13Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.723966.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/723966
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	IR Mass Spec
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used for 15N analysis
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	724410
attribute	NC_GLOBAL	instruments_0_description	String	The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB16/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Isotope-ratio Mass Spectrometer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	469
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Europa Scientific SL-2020 system
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Used to filter sediment
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	724409
attribute	NC_GLOBAL	instruments_1_description	String	A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Pump
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	726
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Multichannel proportioning pump
attribute	NC_GLOBAL	instruments_2_acronym	String	MIMS
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Used to measure dissolved gas
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	724407
attribute	NC_GLOBAL	instruments_2_description	String	Membrane-introduction mass spectrometry (MIMS) is a method of introducing analytes into the mass spectrometer's vacuum chamber via a semipermeable membrane.
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Membrane Inlet Mass Spectrometer
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	661606
attribute	NC_GLOBAL	instruments_2_supplied_name	String	MIMS
attribute	NC_GLOBAL	instruments_3_acronym	String	CFA
attribute	NC_GLOBAL	instruments_3_dataset_instrument_description	String	Used to measure continuous flow rate
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	724408
attribute	NC_GLOBAL	instruments_3_description	String	A sample is injected into a flowing carrier solution passing rapidly through small-bore tubing.
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Continuous Flow Analyzer
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	661968
attribute	NC_GLOBAL	instruments_3_supplied_name	String	Continuous Flow Analyzer
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, chemical, data, dataset, date, description, dmo, east, East_SE, erddap, management, mouth, Mouth_SE, oceanography, office, preliminary, value, Value_Description, west, West_SE, year
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/723966/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/723966
attribute	NC_GLOBAL	param_mapping	String	{'723966': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/723966/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	National Science Foundation
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	NSF-DEB
attribute	NC_GLOBAL	people_0_person_name	String	Dr Behzad Mortazavi
attribute	NC_GLOBAL	people_0_person_nid	String	491316
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Florida State University
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	FSU - EOAS
attribute	NC_GLOBAL	people_1_person_name	String	Dr William C. Burnett
attribute	NC_GLOBAL	people_1_person_nid	String	491315
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	National Science Foundation
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	NSF-DEB
attribute	NC_GLOBAL	people_2_person_name	String	Dr Behzad Mortazavi
attribute	NC_GLOBAL	people_2_person_nid	String	491316
attribute	NC_GLOBAL	people_2_role	String	Contact
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	people_3_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_3_person_name	String	Hannah Ake
attribute	NC_GLOBAL	people_3_person_nid	String	650173
attribute	NC_GLOBAL	people_3_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	project	String	LittleLagoonGroundwater
attribute	NC_GLOBAL	projects_0_acronym	String	LittleLagoonGroundwater
attribute	NC_GLOBAL	projects_0_description	String	This project investigated the link between submarine groundwater discharge (SGD) and microalgal dynamics in Little Lagoon, Alabama.  In contrast to most near-shore environments, it is fully accessible; has no riverine inputs; and is large enough to display ecological diversity (c. 14x 0.75 km) yet small enough to be comprehensively sampled on appropriate temporal and spatial scales. The PIs have previously demonstrated that the lagoon is a hot-spot for toxic blooms of the diatom Pseudo-nitzchia spp. that are correlated with discharge from the surficial aquifer. This project assessed variability in SGD, the dependence of benthic nutrient fluxes on microphytobenthos (MPB) abundance and productivity, and the response of the phytoplankton to nutrient enrichment and dilution. The work integrated multiple temporal and spatial scales and demonstrated both the relative importance of SGD vs. benthic recycling as a source of nutrients, and the role of SGD in structuring the microalgal community. (paraphrased from Award abstract)
attribute	NC_GLOBAL	projects_0_end_date	String	2014-08
attribute	NC_GLOBAL	projects_0_geolocation	String	southern Alabama, east of Mobile
attribute	NC_GLOBAL	projects_0_name	String	Groundwater Discharge, Benthic Coupling and Microalgal Community Structure in a Shallow Coastal Lagoon
attribute	NC_GLOBAL	projects_0_project_nid	String	491318
attribute	NC_GLOBAL	projects_0_start_date	String	2010-03
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Denitrification and DNRA data from Little Lagoon, Alabama collected from 2012-2013
attribute	NC_GLOBAL	title	String	[Denitrification and DNRA] - Denitrification and DNRA data from Little Lagoon, Alabama collected from 2012-2013 (Groundwater Discharge, Benthic Coupling and Microalgal Community Structure in a Shallow Coastal Lagoon)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Year		byte
attribute	Year	_FillValue	byte	127
attribute	Year	actual_range	byte	1, 2
attribute	Year	bcodmo_name	String	unknown
attribute	Year	description	String	Year ID that samples were taken
attribute	Year	long_name	String	Year
attribute	Year	units	String	unitless
variable	Value_Description		String
attribute	Value_Description	bcodmo_name	String	unknown
attribute	Value_Description	description	String	Description of the measurment taken; description includes relevant units for each sample taken; Descriptions include: DIN:DIP = ratio of dissolved inorganic nitrogen to dissolved inorganic phosphate; Denitrification = Denitrification; p14 ambient denitrification = ambient denitrification rates; DNRA = dissimilatory nitrate reduction to ammonium; D15 denitrification = denitrification from added heavy labeled isotope.
attribute	Value_Description	long_name	String	Value Description
attribute	Value_Description	units	String	unitless
variable	Date		String
attribute	Date	bcodmo_name	String	date
attribute	Date	description	String	Month and day that samples were taken; MMM-DD
attribute	Date	long_name	String	Date
attribute	Date	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	Date	units	String	unitless
variable	East		float
attribute	East	_FillValue	float	NaN
attribute	East	actual_range	float	-55.13, 5072.2
attribute	East	bcodmo_name	String	N
attribute	East	description	String	Denitrification and DNRA values collected at the East site; location of site is 30.253347, -87.724729
attribute	East	long_name	String	East
attribute	East	units	String	umol N m-2 hr-1; umol N m-2 d-1; mmol NH4+ m-2 d-1
variable	East_SE		float
attribute	East_SE	_FillValue	float	NaN
attribute	East_SE	actual_range	float	0.0, 1260.2
attribute	East_SE	bcodmo_name	String	N
attribute	East_SE	description	String	Standard error of denitrification and DNRA values collected at the East site
attribute	East_SE	long_name	String	East SE
attribute	East_SE	units	String	umol N m-2 hr-1; umol N m-2 d-1; mmol NH4+ m-2 d-1
variable	Mouth		float
attribute	Mouth	_FillValue	float	NaN
attribute	Mouth	actual_range	float	-52.28, 2549.4
attribute	Mouth	bcodmo_name	String	N
attribute	Mouth	description	String	Denitrification and DNRA values collected at the Mouth site; location of site is 30.243683, -87.738407
attribute	Mouth	long_name	String	Mouth
attribute	Mouth	units	String	umol N m-2 hr-1; umol N m-2 d-1; mmol NH4+ m-2 d-1
variable	Mouth_SE		float
attribute	Mouth_SE	_FillValue	float	NaN
attribute	Mouth_SE	actual_range	float	0.0, 458.2
attribute	Mouth_SE	bcodmo_name	String	N
attribute	Mouth_SE	description	String	Standard error of denitrification and DNRA values collected at the Mouth site
attribute	Mouth_SE	long_name	String	Mouth SE
attribute	Mouth_SE	units	String	umol N m-2 hr-1; umol N m-2 d-1; mmol NH4+ m-2 d-1
variable	West		float
attribute	West	_FillValue	float	NaN
attribute	West	actual_range	float	-77.78, 5678.0
attribute	West	bcodmo_name	String	N
attribute	West	description	String	Denitrification and DNRA values collected at the West site; location of site is 30.247181, -87.767856
attribute	West	long_name	String	West
attribute	West	units	String	umol N m-2 hr-1; umol N m-2 d-1; mmol NH4+ m-2 d-1
variable	West_SE		float
attribute	West_SE	_FillValue	float	NaN
attribute	West_SE	actual_range	float	0.0, 1181.1
attribute	West_SE	bcodmo_name	String	N
attribute	West_SE	description	String	Standard error of denitrification and DNRA values collected at the West site
attribute	West_SE	long_name	String	West SE
attribute	West_SE	units	String	umol N m-2 hr-1; umol N m-2 d-1; mmol NH4+ m-2 d-1

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.