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     data   graph     files  public Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2
variability (Seaweed OA Resilience project)
   ?     I   M   background (external link) RSS BCO-DMO bcodmo_dataset_732177

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Culture pots were placed in large thermally insulated coolers in a
temperature-controlled water bath at 15\u00b0 C under saturating illumination
of ~550 \u00b5moles photons/m^2/s on a 12:12 L:D cycle. pCO2 treatments were
supplied to closed culture pots by use of a gas mixing system combining
Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9%
oxygen and the balance being Nitrogen.

Carbonate chemistry parameters were measured by sampling pH and total
alkalinity (TA) of water samples. Seawater samples from each culture pot
during all 3 trials were collected 2-3 times per week in 50 ml Falcon tubes to
monitor the average and variability of experimental treatment conditions.
Water samples were measured usually within 1-2 hours of sample collection. pH
was determined using the m-cresol indicator dye method in a spectrophotometer
(Dickson et al. 2007). TA samples were analyzed by potentiometric titration
coupled to a pH electrode calibrated using certified reference material (CRM)
from the Dickson laboratory at Scripps Oceanographic Institute and the pH
electrode calibrated using TRIS buffer (Dickson et al. 2007). TA and carbonate
parameters were calculated from potentiometric titration data and
spectrophotometric pH data.

Carbonate chemistry parameters (CO2 concentration, CO2 partial pressure, CO2
fugacity, HCO3, CO3, DIC, Omega Aragonite, Omega Calcite) and total scale pH
at in situ temperature were calculated using the seacarb package (V3.0.14) in
R (Lavigne et al. 2011).
attribute NC_GLOBAL awards_0_award_nid String 55177
attribute NC_GLOBAL awards_0_award_number String OCE-1316198
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=1316198 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Dr David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Carbonate chemistry pCO2 - Ulva
Carbonate Chemistry in culture pots with Ulva testing effects of variability
PI's: J. Kubler, S. Dudgeon (CSU-Northridge)
version: 2018-03-22
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.2d 13 Jun 2019
attribute NC_GLOBAL date_created String 2018-03-27T19:54:55Z
attribute NC_GLOBAL date_modified String 2019-06-03T18:23:00Z
attribute NC_GLOBAL defaultDataQuery String &time
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.732177.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/732177 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String UV Spectrophotometer-Shimadzu
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to measure pH total scale at 25C.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 732213
attribute NC_GLOBAL instruments_0_description String The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB20/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String UV Spectrophotometer-Shimadzu
attribute NC_GLOBAL instruments_0_instrument_nid String 595
attribute NC_GLOBAL instruments_0_supplied_name String Shimadzu UV-2450 UV-visible spectrophotometer
attribute NC_GLOBAL instruments_1_acronym String Multi Parameter Bench Meter
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used to measure�pH, temperature and dissolved oxygen.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 732212
attribute NC_GLOBAL instruments_1_description String An analytical instrument that can measure multiple parameters, such as pH, EC, TDS, DO and Temperature with one device.
attribute NC_GLOBAL instruments_1_instrument_name String Multi Parameter Bench Meter
attribute NC_GLOBAL instruments_1_instrument_nid String 680
attribute NC_GLOBAL instruments_1_supplied_name String Thermo Fisher Orion Star 329
attribute NC_GLOBAL instruments_2_acronym String Automatic titrator
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Used to measure total alkalinity.
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 732214
attribute NC_GLOBAL instruments_2_description String Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB12/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Automatic titrator
attribute NC_GLOBAL instruments_2_instrument_nid String 682
attribute NC_GLOBAL instruments_2_supplied_name String Mettler Toledo T50 equipped with Rondolino
attribute NC_GLOBAL instruments_3_acronym String MFC
attribute NC_GLOBAL instruments_3_dataset_instrument_description String Settings: Nitrogen, 1 L/min; Oxygen, 250 ml/min; CO2, 2 ml/min.
attribute NC_GLOBAL instruments_3_dataset_instrument_nid String 732211
attribute NC_GLOBAL instruments_3_description String Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases
attribute NC_GLOBAL instruments_3_instrument_name String Mass Flow Controller
attribute NC_GLOBAL instruments_3_instrument_nid String 712
attribute NC_GLOBAL instruments_3_supplied_name String Qubit Systems Mass Flow Controllers (MFC)
attribute NC_GLOBAL instruments_4_acronym String Aquarium chiller
attribute NC_GLOBAL instruments_4_dataset_instrument_nid String 732210
attribute NC_GLOBAL instruments_4_description String Immersible or in-line liquid cooling device, usually with temperature control.
attribute NC_GLOBAL instruments_4_instrument_name String Aquarium chiller
attribute NC_GLOBAL instruments_4_instrument_nid String 522982
attribute NC_GLOBAL instruments_4_supplied_name String Temperature control chiller: Aqua Logic Cyclone Chiller
attribute NC_GLOBAL keywords String alk, altimetry, aragonite, average, bco, bco-dmo, biological, calcite, carbon, carbon dioxide, carbonate, chemical, co2, co2weights, co3, culture, Culture_Pot_ID, data, dataset, date, days, days_diff, dic, diff, dioxide, dmo, erddap, exp, Exp_Trial, fCO2, h25, hco3, hin, identifier, laboratory, management, oceanography, office, omega, OmegaAragonite, OmegaCalcite, pCO2, pCO2weights, pH25, pHin, pot, preliminary, sal, Sal_in, satellite, statistics, Temp_in, temperature, time, trial, wt_avg_pCO2, wt_sd_pCO2
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/732177 (external link)
attribute NC_GLOBAL param_mapping String {'732177': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/732177/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String California State University Northridge
attribute NC_GLOBAL people_0_affiliation_acronym String CSU-Northridge
attribute NC_GLOBAL people_0_person_name String Dr Janet E Kubler
attribute NC_GLOBAL people_0_person_nid String 51681
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String California State University Northridge
attribute NC_GLOBAL people_1_affiliation_acronym String CSU-Northridge
attribute NC_GLOBAL people_1_person_name String Dr Steve Dudgeon
attribute NC_GLOBAL people_1_person_nid String 51682
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Nancy Copley
attribute NC_GLOBAL people_2_person_nid String 50396
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae
attribute NC_GLOBAL projects_0_acronym String Seaweed OA Resilience
attribute NC_GLOBAL projects_0_description String Benthic macroalgae contribute to intensely productive near shore ecosystems and little is known about the potential effects of ocean acidification on non-calcifying macroalgae. K�bler and Dudgeon will test hypotheses about two macroalgae, Ulva spp. and Plocamium cartilagineum, which, for different reasons, are hypothesized to be more productive and undergo ecological expansions under predicted changes in ocean chemistry. They have designed laboratory culture-based experiments to quantify the scope for response to ocean acidification in Plocamium, which relies solely on diffusive uptake of CO2, and populations of Ulva spp., which have an inducible concentrating mechanism (CCM). The investigators will culture these algae in media equilibrated at 8 different pCO2 levels ranging from 380 to 940 ppm to address three key hypotheses. The first is that macroalgae (such as Plocamium cartilagineum) that are not able to acquire inorganic carbon in changed form will benefit, in terms of photosynthetic and growth rates, from ocean acidification. There is little existing data to support this common assumption. The second hypothesis is that enhanced growth of Ulva sp. under OA will result from the energetic savings from down regulating the CCM, rather than from enhanced photosynthesis per se. Their approach will detect existing genetic variation for adaptive plasticity. The third key hypothesis to be addressed in short-term culture experiments is that there will be a significant interaction between ocean acidification and nitrogen limited growth of Ulva spp., which are indicator species of eutrophication. K�bler and Dudgeon will be able to quantify the individual effects of ocean acidification and nitrogenous nutrient addition on Ulva spp. and also, the synergistic effects, which will inevitably apply in many highly productive, shallow coastal areas. The three hypotheses being addressed have been broadly identified as urgent needs in our growing understanding of the impacts of ocean acidification.
attribute NC_GLOBAL projects_0_end_date String 2016-05
attribute NC_GLOBAL projects_0_geolocation String Temperate coastal waters of the USA (30 - 45 N latitude, -66 to -88 W and -117 to -125 W longitude)
attribute NC_GLOBAL projects_0_name String Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae
attribute NC_GLOBAL projects_0_project_nid String 2275
attribute NC_GLOBAL projects_0_start_date String 2013-06
attribute NC_GLOBAL publisher_name String Nancy Copley
attribute NC_GLOBAL publisher_role String BCO-DMO Data Manager(s)
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v29
attribute NC_GLOBAL summary String This dataset reports the carbonate chemistry along with temperature, salinity and pH of Ulva lactuca grown in closed culture pots at varying pCO2 levels.
attribute NC_GLOBAL title String Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability (Seaweed OA Resilience project)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5-beta
variable Culture_Pot_ID   byte  
attribute Culture_Pot_ID _FillValue byte 127
attribute Culture_Pot_ID actual_range byte 1, 10
attribute Culture_Pot_ID description String Identification number of culture
attribute Culture_Pot_ID ioos_category String Identifier
attribute Culture_Pot_ID long_name String Culture Pot ID
attribute Culture_Pot_ID units String unitless
variable Exp_Trial   byte  
attribute Exp_Trial _FillValue byte 127
attribute Exp_Trial actual_range byte 2, 4
attribute Exp_Trial description String Trial consisting of a range of average of pCO2 levels crossed with either or low or high variability around the average pCO2 effect
attribute Exp_Trial ioos_category String Unknown
attribute Exp_Trial long_name String Exp Trial
attribute Exp_Trial units String unitless
variable Date   String  
attribute Date description String Date formatted as yyyy-mm-dd
attribute Date ioos_category String Time
attribute Date long_name String Date
attribute Date source_name String Date
attribute Date units String unitless
variable days_diff   byte  
attribute days_diff _FillValue byte 127
attribute days_diff actual_range byte 1, 6
attribute days_diff colorBarMaximum double 10.0
attribute days_diff colorBarMinimum double -10.0
attribute days_diff description String Number of days between present and subsequent seawater samples to measure carbonate chemistry parameters
attribute days_diff ioos_category String Time
attribute days_diff long_name String Days Diff
attribute days_diff units String days
variable pCO2weights   double  
attribute pCO2weights _FillValue double NaN
attribute pCO2weights actual_range double 7.689138184, 7279.481028
attribute pCO2weights description String Product of days_diff and pCO2: used to generated weighted estimates of the average and standard deviation carbonate chemistry parameters for each culture pot in each experimental trial
attribute pCO2weights ioos_category String CO2
attribute pCO2weights long_name String P CO2weights
attribute pCO2weights units String microatmosphere days (�atm.days)
variable wt_avg_pCO2   short  
attribute wt_avg_pCO2 _FillValue short 32767
attribute wt_avg_pCO2 actual_range short 248, 1001
attribute wt_avg_pCO2 description String Weighted average of pCO2 partial pressure in seawater tanks
attribute wt_avg_pCO2 ioos_category String CO2
attribute wt_avg_pCO2 long_name String Wt Avg P CO2
attribute wt_avg_pCO2 units String microatmospheres (�atm)
variable wt_sd_pCO2   float  
attribute wt_sd_pCO2 _FillValue float NaN
attribute wt_sd_pCO2 actual_range float 36.22, 1827.8
attribute wt_sd_pCO2 colorBarMaximum double 50.0
attribute wt_sd_pCO2 colorBarMinimum double 0.0
attribute wt_sd_pCO2 description String Weighted standard deviation of pCO2 partial pressure in seawater tanks
attribute wt_sd_pCO2 ioos_category String Statistics
attribute wt_sd_pCO2 long_name String Wt Sd P CO2
attribute wt_sd_pCO2 units String microatmospheres (�atm)
variable Sal_in   float  
attribute Sal_in _FillValue float NaN
attribute Sal_in actual_range float 3.73, 34.09
attribute Sal_in description String Salinity in situ
attribute Sal_in ioos_category String Unknown
attribute Sal_in long_name String Sal In
attribute Sal_in units String parts per thousand (ppt)
variable Temp_in   float  
attribute Temp_in _FillValue float NaN
attribute Temp_in actual_range float 15.0, 16.3
attribute Temp_in description String Temperature in situ
attribute Temp_in ioos_category String Unknown
attribute Temp_in long_name String Temp In
attribute Temp_in units String degrees Celsius
variable pH25   float  
attribute pH25 _FillValue float NaN
attribute pH25 actual_range float 7.44, 9.39
attribute pH25 description String pH in Total scale at 25 deg C
attribute pH25 ioos_category String Unknown
attribute pH25 long_name String P H25
attribute pH25 units String unitless
variable pHin   float  
attribute pHin _FillValue float NaN
attribute pHin actual_range float 7.57, 9.6
attribute pHin description String pH-Total scale in situ temperature
attribute pHin ioos_category String Unknown
attribute pHin long_name String P Hin
attribute pHin units String unitless
variable CO2   float  
attribute CO2 _FillValue float NaN
attribute CO2 actual_range float 0.07, 76.7
attribute CO2 description String Carbon dioxide concentration
attribute CO2 ioos_category String CO2
attribute CO2 long_name String CO2
attribute CO2 units String micromoles/kilogram
variable pCO2   float  
attribute pCO2 _FillValue float NaN
attribute pCO2 actual_range float 1.92, 1730.54
attribute pCO2 description String CO2 partial pressure
attribute pCO2 ioos_category String CO2
attribute pCO2 long_name String P CO2
attribute pCO2 units String microatmospheres (�atm)
variable fCO2   float  
attribute fCO2 _FillValue float NaN
attribute fCO2 actual_range float 1.92, 1724.38
attribute fCO2 description String CO2 fugacity
attribute fCO2 ioos_category String CO2
attribute fCO2 long_name String F CO2
attribute fCO2 units String microatmospheres (�atm)
variable HCO3   float  
attribute HCO3 _FillValue float NaN
attribute HCO3 actual_range float 327.51, 2610.53
attribute HCO3 description String Bicarbonate ion concentration
attribute HCO3 ioos_category String CO2
attribute HCO3 long_name String HCO3
attribute HCO3 units String micromoles/kilogram
variable CO3   float  
attribute CO3 _FillValue float NaN
attribute CO3 actual_range float 26.47, 948.02
attribute CO3 description String Carbonate ion concentration
attribute CO3 ioos_category String CO2
attribute CO3 long_name String CO3
attribute CO3 units String micromoles/kilogram
variable DIC   float  
attribute DIC _FillValue float NaN
attribute DIC actual_range float 1275.6, 2769.91
attribute DIC description String Total dissolved inorganic carbon
attribute DIC ioos_category String Unknown
attribute DIC long_name String DIC
attribute DIC units String micromoles/kilogram
variable ALK   float  
attribute ALK _FillValue float NaN
attribute ALK actual_range float 2053.86, 3033.89
attribute ALK description String Total Alkalinity measured
attribute ALK ioos_category String Unknown
attribute ALK long_name String ALK
attribute ALK units String micromoles/kilogram
variable OmegaAragonite   float  
attribute OmegaAragonite _FillValue float NaN
attribute OmegaAragonite actual_range float 0.42, 14.65
attribute OmegaAragonite description String Aragonite saturation state
attribute OmegaAragonite ioos_category String Unknown
attribute OmegaAragonite long_name String Omega Aragonite
attribute OmegaAragonite units String unitless
variable OmegaCalcite   float  
attribute OmegaCalcite _FillValue float NaN
attribute OmegaCalcite actual_range float 0.75, 22.87
attribute OmegaCalcite description String Calcite Saturation State
attribute OmegaCalcite ioos_category String Unknown
attribute OmegaCalcite long_name String Omega Calcite
attribute OmegaCalcite units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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