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     data   graph     files  public [Ulva: Photosynthesis and respiration] - Rates of photosynthesis and respiration by Ulva
exposed to different average and variability of pCO2 (Seaweed OA Resilience project) (Ocean
Acidification: Scope for Resilience to Ocean Acidification in Macroalgae)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Measurements of photosynthesis and respiration of Ulva lactuca occurred at the
end of 3 weeks of acclimation to various levels of ocean acidification at
15\u00b0C. Small circular sections of U. lactuca thalli used for measurements
were sampled with a cork borer the evening before measurement and returned to
culture conditions overnight to allow for wound healing. Plant sections used
to estimate rates of photosynthesis and respiration from oxygen exchange
measurements were placed in small white, mesh threaded bags designed to fit
the diameter of the cuvette chamber so that the plant material was oriented
perpendicularly to the light source provided in the Qubit Systems Dissolved
Oxygen package. Prior to introducing plants to the cuvette chamber, oxygen
exchange between the stirred seawater and electrode system (i.e., oxygen
consumption by the electrode) was monitored to adjust for its effect on
subsequent measurements in the presence of plant material. Sections were
transferred to the cuvette containing a magnetic stirring bar and 3 ml of
seawater corresponding to the average pCO2 level in their particular culture
pot and placed in darkness for 10-15 minutes prior to beginning measurement.
Once measurements began, output of oxygen exchange was monitored until a
steady state of oxygen exchange was attained (typically within 10 minutes) in
either darkness or light. Photosynthesis and respiration were measured at
15\u00b0C and ~550, and 0, \u00b5mole photons m-2 s-1, respectively; the same
temperature and light conditions as in growth culture. Respiration was
measured both following a \u226510 minute acclimation period in darkness prior
to light exposure (dark respiration) and again in darkness immediately
following exposure to the saturating light intensity (as an estimate of
respiration that occurs in the light). Following these measurements in
seawater at their culture pCO2 level, the seawater in the cuvette was replaced
with standard air-saturated seawater at 15\u00b0C and the measurement process
described above was repeated for measurements of respiration in the dark and
\u2018light\u2019 and photosynthesis. After measurements of gas exchange, the
mass of plant tissue was measured on an analytical balance.

Note: Trial 1 was a pilot test of culture system and methodological procedures
so was not used for data collection.
attribute NC_GLOBAL awards_0_award_nid String 55177
attribute NC_GLOBAL awards_0_award_number String OCE-1316198
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=1316198 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Ulva_PS_Resp_Cult_AirSat - Ulva
Rates of photosynthesis and respiration exposed to different average and variability of pCO2
PI's: J. Kubler, S. Dudgeon (CSU-Northridge)
version: 2018-03-26
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2018-03-28T20:40:42Z
attribute NC_GLOBAL date_modified String 2019-06-03T17:29:10Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.732625.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/732625 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Dissolved Oxygen Sensor
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to measure oxygen, photosynthesis, and respiration.http://www.qubitbiology.com/ox1lp-dissolved-oxygen/
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 732633
attribute NC_GLOBAL instruments_0_description String An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed
attribute NC_GLOBAL instruments_0_instrument_name String Dissolved Oxygen Sensor
attribute NC_GLOBAL instruments_0_instrument_nid String 705
attribute NC_GLOBAL instruments_0_supplied_name String Qubit Systems Dissolved Oxygen package (Q-box OX1LP)
attribute NC_GLOBAL instruments_1_acronym String Scale
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 732631
attribute NC_GLOBAL instruments_1_description String An instrument used to measure weight or mass.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB13/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Scale
attribute NC_GLOBAL instruments_1_instrument_nid String 714
attribute NC_GLOBAL instruments_1_supplied_name String Mettler Toledo AG204 Delta Range Analytical Balance
attribute NC_GLOBAL keywords String average, bco, bco-dmo, biological, carbon, carbon dioxide, chemical, co2, data, dataset, dioxide, dmo, dresp, DRespA, DRespC, erddap, fresh, Fresh_Weight, label, lresp, LRespA, LRespC, management, oceanography, off1, Off1A, Off1C, off2, Off2A, Off2C, office, OnA, OnC, pCO2_avg, pCO2_sd, pmax, PmaxA, PmaxC, preliminary, weight
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/732625/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/732625 (external link)
attribute NC_GLOBAL param_mapping String {'732625': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/732625/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String California State University Northridge
attribute NC_GLOBAL people_0_affiliation_acronym String CSU-Northridge
attribute NC_GLOBAL people_0_person_name String Dr Janet E Kubler
attribute NC_GLOBAL people_0_person_nid String 51681
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String California State University Northridge
attribute NC_GLOBAL people_1_affiliation_acronym String CSU-Northridge
attribute NC_GLOBAL people_1_person_name String Dr Steve Dudgeon
attribute NC_GLOBAL people_1_person_nid String 51682
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Nancy Copley
attribute NC_GLOBAL people_2_person_nid String 50396
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String Seaweed OA Resilience
attribute NC_GLOBAL projects_0_acronym String Seaweed OA Resilience
attribute NC_GLOBAL projects_0_description String Benthic macroalgae contribute to intensely productive near shore ecosystems and little is known about the potential effects of ocean acidification on non-calcifying macroalgae. Kübler and Dudgeon will test hypotheses about two macroalgae, Ulva spp. and Plocamium cartilagineum, which, for different reasons, are hypothesized to be more productive and undergo ecological expansions under predicted changes in ocean chemistry. They have designed laboratory culture-based experiments to quantify the scope for response to ocean acidification in Plocamium, which relies solely on diffusive uptake of CO2, and populations of Ulva spp., which have an inducible concentrating mechanism (CCM). The investigators will culture these algae in media equilibrated at 8 different pCO2 levels ranging from 380 to 940 ppm to address three key hypotheses. The first is that macroalgae (such as Plocamium cartilagineum) that are not able to acquire inorganic carbon in changed form will benefit, in terms of photosynthetic and growth rates, from ocean acidification. There is little existing data to support this common assumption. The second hypothesis is that enhanced growth of Ulva sp. under OA will result from the energetic savings from down regulating the CCM, rather than from enhanced photosynthesis per se. Their approach will detect existing genetic variation for adaptive plasticity. The third key hypothesis to be addressed in short-term culture experiments is that there will be a significant interaction between ocean acidification and nitrogen limited growth of Ulva spp., which are indicator species of eutrophication. Kübler and Dudgeon will be able to quantify the individual effects of ocean acidification and nitrogenous nutrient addition on Ulva spp. and also, the synergistic effects, which will inevitably apply in many highly productive, shallow coastal areas. The three hypotheses being addressed have been broadly identified as urgent needs in our growing understanding of the impacts of ocean acidification.
attribute NC_GLOBAL projects_0_end_date String 2016-05
attribute NC_GLOBAL projects_0_geolocation String Temperate coastal waters of the USA (30 - 45 N latitude, -66 to -88 W and -117 to -125 W longitude)
attribute NC_GLOBAL projects_0_name String Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae
attribute NC_GLOBAL projects_0_project_nid String 2275
attribute NC_GLOBAL projects_0_start_date String 2013-06
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String This dataset includes rates of photosynthesis and respiration by Ulva during culture experiments grown at 15 degrees C under various pCO2 levels, during May through July 2015.
attribute NC_GLOBAL title String [Ulva: Photosynthesis and respiration] - Rates of photosynthesis and respiration by Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project) (Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Label   String  
attribute Label bcodmo_name String sample
attribute Label description String sample identifier formatted as: trial-culture pot.plant replicate numbers
attribute Label long_name String Label
attribute Label nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Label units String unitless
variable pCO2_avg   short  
attribute pCO2_avg _FillValue short 32767
attribute pCO2_avg actual_range short 248, 962
attribute pCO2_avg bcodmo_name String pCO2
attribute pCO2_avg description String Average pCO2 partial pressure in seawater tanks
attribute pCO2_avg long_name String P CO2 Avg
attribute pCO2_avg nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/ (external link)
attribute pCO2_avg units String microatmospheres (µatm)
variable pCO2_sd   float  
attribute pCO2_sd _FillValue float NaN
attribute pCO2_sd actual_range float 36.22, 1827.8
attribute pCO2_sd bcodmo_name String pCO2
attribute pCO2_sd colorBarMaximum double 50.0
attribute pCO2_sd colorBarMinimum double 0.0
attribute pCO2_sd description String Variability of pCO2 partial pressure - standard deviation
attribute pCO2_sd long_name String P CO2 Sd
attribute pCO2_sd nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/ (external link)
attribute pCO2_sd units String microatmospheres (µatm)
variable Off1C   float  
attribute Off1C _FillValue float NaN
attribute Off1C actual_range float -0.0026844, -2.7471E-5
attribute Off1C bcodmo_name String respiration
attribute Off1C description String slope of oxygen exchange during first dark period in pCO2 culture seawater
attribute Off1C long_name String Off1 C
attribute Off1C units String milligrams O2/liter/second (mg L-1 s-1)
variable OnC   float  
attribute OnC _FillValue float NaN
attribute OnC actual_range float -5.5738E-4, 0.008294
attribute OnC bcodmo_name String respiration
attribute OnC description String slope of oxygen exchange during light period in pCO2 culture seawater
attribute OnC long_name String On C
attribute OnC units String milligrams O2/liter/second (mg L-1 s-1)
variable Off2C   float  
attribute Off2C _FillValue float NaN
attribute Off2C actual_range float -0.0020771, -3.3332E-4
attribute Off2C bcodmo_name String respiration
attribute Off2C description String slope of oxygen exchange during second dark period in pCO2 culture seawater
attribute Off2C long_name String Off2 C
attribute Off2C units String milligrams O2/liter/second (mg L-1 s-1)
variable Off1A   float  
attribute Off1A _FillValue float NaN
attribute Off1A actual_range float -0.0035805, -2.8366E-4
attribute Off1A bcodmo_name String respiration
attribute Off1A description String slope of oxygen exchange during first dark period in aerated standard seawater
attribute Off1A long_name String Off1 A
attribute Off1A units String milligrams O2/liter/second (mg L-1 s-1)
variable OnA   float  
attribute OnA _FillValue float NaN
attribute OnA actual_range float -1.71393E-4, 0.0070203
attribute OnA bcodmo_name String respiration
attribute OnA description String slope of oxygen exchange during light period in aerated standard seawater
attribute OnA long_name String On A
attribute OnA units String milligrams O2/liter/second (mg L-1 s-1)
variable Off2A   float  
attribute Off2A _FillValue float NaN
attribute Off2A actual_range float -0.0015834, -2.3486E-4
attribute Off2A bcodmo_name String respiration
attribute Off2A description String slope of oxygen exchange during second dark period in aerated standard seawater
attribute Off2A long_name String Off2 A
attribute Off2A units String milligrams O2/liter/second (mg L-1 s-1)
variable Fresh_Weight   float  
attribute Fresh_Weight _FillValue float NaN
attribute Fresh_Weight actual_range float 0.0124, 0.365
attribute Fresh_Weight bcodmo_name String mass
attribute Fresh_Weight description String Mass of tissue sample
attribute Fresh_Weight long_name String Fresh Weight
attribute Fresh_Weight units String grams
variable DRespC   double  
attribute DRespC _FillValue double NaN
attribute DRespC actual_range double -3.375149402, -0.040398529
attribute DRespC bcodmo_name String respiration
attribute DRespC description String Dark respiration rate during first dark period in pCO2 culture seawater
attribute DRespC long_name String DResp C
attribute DRespC units String mol O2/g/min NA
variable PmaxC   double  
attribute PmaxC _FillValue double NaN
attribute PmaxC actual_range double -0.687557068, 8.886336207
attribute PmaxC bcodmo_name String Pmax
attribute PmaxC description String Maximum rate of photosynthesis in pCO2 culture seawater
attribute PmaxC long_name String Pmax C
attribute PmaxC units String mol O2/g/min NA
variable LRespC   double  
attribute LRespC _FillValue double NaN
attribute LRespC actual_range double -3.103237052, -0.164494121
attribute LRespC bcodmo_name String respiration
attribute LRespC description String Estimated respiration in the light in pCO2 culture seawater
attribute LRespC long_name String LResp C
attribute LRespC units String mol O2/g/min NA
variable DRespA   double  
attribute DRespA _FillValue double NaN
attribute DRespA actual_range double -3.323483911, -0.152730795
attribute DRespA bcodmo_name String respiration
attribute DRespA description String Dark respiration rate during first dark period in aerated standard seawater
attribute DRespA long_name String DResp A
attribute DRespA units String mol O2/g/min NA
variable PmaxA   double  
attribute PmaxA _FillValue double NaN
attribute PmaxA actual_range double -0.211422715, 9.567512376
attribute PmaxA bcodmo_name String Pmax
attribute PmaxA description String Maximum rate of photosynthesis in aerated standard seawater
attribute PmaxA long_name String Pmax A
attribute PmaxA units String mol O2/g/min NA
variable LRespA   double  
attribute LRespA _FillValue double NaN
attribute LRespA actual_range double -1.657299919, -0.15860043
attribute LRespA bcodmo_name String respiration
attribute LRespA description String Estimated respiration in the light in aerated standard seawater
attribute LRespA long_name String LResp A
attribute LRespA units String mol O2/g/min NA

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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