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     data   graph     files  public Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and
pCO2 and temperature conditions
   ?     I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_733238

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Plocamium cartilagineum was collected from Catalina Island in June - Nov. 2014
and Jan. 2015.

Culture pots were placed in large thermally insulated coolers in a
temperature-controlled water bath at either 15 or 20\u00b0C under saturating
illumination of ~150 \u00b5moles photons/m^2/s. pCO2 treatments were supplied
to closed culture pots by use of a gas mixing system combining Nitrogen,
Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and
the balance being Nitrogen.

Measurements of\u00a0chlorophyll fluorescence of photosystem II were made
using a pulse amplitude modulated (PAM) fluorometer in situ in culture pots.
All samples were dark-adapted prior to measurement by placing a leaf clip in
the closed position over the area to be sampled for 5 minutes prior to
measurement. For fluorescence measurements, the fiber optic probe was placed
directly on the leaf clip perpendicular to the sample. The leaf clip was then
opened and a rapid light curve program function was initiated to measure
fluorescence at each of 9 photon flux densities (PFD\u2019s). PFD\u2019s of
the light curve generated by the fluorometer were calibrated using an external
LiCor light meter. Following measurement, the leaf clip was removed and the
sample remained in the culture chamber.\u00a0

Data were downloaded from the Diving PAM using WinControl version 3 software
for Diving PAM.

Note: Trial 1 was a pilot test of culture system and methodological procedures
so was not used for data collection in the testing of hypotheses.

See Supplemental Files for a table of culture conditions for each of the 8
trials (pdf).
attribute NC_GLOBAL awards_0_award_nid String 55177
attribute NC_GLOBAL awards_0_award_number String OCE-1316198
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=1316198 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Rapid Light Curves - PAM
Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions
with pulse amplitude modulated (PAM) fluorometer
PI: S. Dudgeon, J. Kubler (CSUN)
version: 2018-04-11
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2018-04-12T12:14:18Z
attribute NC_GLOBAL date_modified String 2019-06-03T17:25:18Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.733238.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/733238 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Fluorometer
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to measure chlorophyll fluorescence
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 733246
attribute NC_GLOBAL instruments_0_description String A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/113/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Fluorometer
attribute NC_GLOBAL instruments_0_instrument_nid String 484
attribute NC_GLOBAL instruments_0_supplied_name String Walz Underwater fluorometer - Diving-PAM, Heinz Walz, Effeltrich, Germany
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, carbon, carbon dioxide, chemical, co2, data, dataset, date, dioxide, dmo, erddap, etr, light, management, npq, oceanography, office, pCO2, plant, Plant_ID, preliminary, rETR, tank, Tank_ID, Temp, temperature, time, trial, week, yield
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/733238/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/733238 (external link)
attribute NC_GLOBAL param_mapping String {'733238': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/733238/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String California State University Northridge
attribute NC_GLOBAL people_0_affiliation_acronym String CSU-Northridge
attribute NC_GLOBAL people_0_person_name String Dr Janet E Kubler
attribute NC_GLOBAL people_0_person_nid String 51681
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String California State University Northridge
attribute NC_GLOBAL people_1_affiliation_acronym String CSU-Northridge
attribute NC_GLOBAL people_1_person_name String Dr Steve Dudgeon
attribute NC_GLOBAL people_1_person_nid String 51682
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Nancy Copley
attribute NC_GLOBAL people_2_person_nid String 50396
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String Seaweed OA Resilience
attribute NC_GLOBAL projects_0_acronym String Seaweed OA Resilience
attribute NC_GLOBAL projects_0_description String Benthic macroalgae contribute to intensely productive near shore ecosystems and little is known about the potential effects of ocean acidification on non-calcifying macroalgae. Kübler and Dudgeon will test hypotheses about two macroalgae, Ulva spp. and Plocamium cartilagineum, which, for different reasons, are hypothesized to be more productive and undergo ecological expansions under predicted changes in ocean chemistry. They have designed laboratory culture-based experiments to quantify the scope for response to ocean acidification in Plocamium, which relies solely on diffusive uptake of CO2, and populations of Ulva spp., which have an inducible concentrating mechanism (CCM). The investigators will culture these algae in media equilibrated at 8 different pCO2 levels ranging from 380 to 940 ppm to address three key hypotheses. The first is that macroalgae (such as Plocamium cartilagineum) that are not able to acquire inorganic carbon in changed form will benefit, in terms of photosynthetic and growth rates, from ocean acidification. There is little existing data to support this common assumption. The second hypothesis is that enhanced growth of Ulva sp. under OA will result from the energetic savings from down regulating the CCM, rather than from enhanced photosynthesis per se. Their approach will detect existing genetic variation for adaptive plasticity. The third key hypothesis to be addressed in short-term culture experiments is that there will be a significant interaction between ocean acidification and nitrogen limited growth of Ulva spp., which are indicator species of eutrophication. Kübler and Dudgeon will be able to quantify the individual effects of ocean acidification and nitrogenous nutrient addition on Ulva spp. and also, the synergistic effects, which will inevitably apply in many highly productive, shallow coastal areas. The three hypotheses being addressed have been broadly identified as urgent needs in our growing understanding of the impacts of ocean acidification.
attribute NC_GLOBAL projects_0_end_date String 2016-05
attribute NC_GLOBAL projects_0_geolocation String Temperate coastal waters of the USA (30 - 45 N latitude, -66 to -88 W and -117 to -125 W longitude)
attribute NC_GLOBAL projects_0_name String Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae
attribute NC_GLOBAL projects_0_project_nid String 2275
attribute NC_GLOBAL projects_0_start_date String 2013-06
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String This dataset includes photosynthetic pigment concentrations in Plocamium cartilagineum grown under various temperatures and CO2 levels, from July 2014 to February 2015. The parameters reported are: the concentrations of Chlorophyll a, Phycoerythrin, Phycocyanin, Allophycocyanin, and total Phycobiliprotein, and the ratio of total Phycobiliprotein to Chlorophyll A.
attribute NC_GLOBAL title String Measurements of fluorescence of photosystem II in Plocamium cartilagineum under various and pCO2 and temperature conditions
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Trial   byte  
attribute Trial _FillValue byte 127
attribute Trial actual_range byte 2, 8
attribute Trial bcodmo_name String replicate
attribute Trial description String Trial number of the experiment
attribute Trial long_name String Trial
attribute Trial units String unitless
variable Tank_ID   byte  
attribute Tank_ID _FillValue byte 127
attribute Tank_ID actual_range byte 0, 9
attribute Tank_ID bcodmo_name String sample
attribute Tank_ID description String Identifying number of culture pot
attribute Tank_ID long_name String Tank ID
attribute Tank_ID nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Tank_ID units String unitless
variable pCO2   short  
attribute pCO2 _FillValue short 32767
attribute pCO2 actual_range short 344, 1053
attribute pCO2 bcodmo_name String pCO2
attribute pCO2 description String Partial pressure of CO2 in solution in culture pot
attribute pCO2 long_name String P CO2
attribute pCO2 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/ (external link)
attribute pCO2 units String atm
variable Temp   byte  
attribute Temp _FillValue byte 127
attribute Temp actual_range byte 15, 20
attribute Temp bcodmo_name String temperature
attribute Temp description String Temperature of seawater in culture pot
attribute Temp long_name String Temperature
attribute Temp nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/ (external link)
attribute Temp units String degrees Celsius (°C)
variable Plant_ID   float  
attribute Plant_ID _FillValue float NaN
attribute Plant_ID actual_range float 1.1, 15.2
attribute Plant_ID bcodmo_name String sample
attribute Plant_ID description String Plant ID number in culture pot
attribute Plant_ID long_name String Plant ID
attribute Plant_ID nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Plant_ID units String unitless
variable ID   String  
attribute ID bcodmo_name String sample
attribute ID description String Unique identifier for every sample; concatenation of trial_tank ID_plant ID_week
attribute ID long_name String ID
attribute ID nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute ID units String unitless
variable Week   byte  
attribute Week _FillValue byte 127
attribute Week actual_range byte 0, 4
attribute Week bcodmo_name String time_point
attribute Week description String Week number of given trial
attribute Week long_name String Week
attribute Week units String unitless
variable date   String  
attribute date bcodmo_name String date_local
attribute date description String date of data collection formatted as yyyy-mm-dd
attribute date long_name String Date
attribute date source_name String date
attribute date time_precision String 1970-01-01
attribute date units String unitless
variable light   short  
attribute light _FillValue short 32767
attribute light actual_range short 0, 427
attribute light bcodmo_name String PAR
attribute light description String Photosynthetically active radiation (PAR)
attribute light long_name String Light
attribute light units String micromole photons/meter^2/second ( µmole photons . m^-2 . s^-1)
variable F   float  
attribute F _FillValue float NaN
attribute F actual_range float 0.0, 334.0
attribute F bcodmo_name String fluorescence
attribute F description String Relative fluorescence of photosystem II
attribute F long_name String F
attribute F nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute F units String unitless
variable Fm   float  
attribute Fm _FillValue float NaN
attribute Fm actual_range float 0.0, 454.0
attribute Fm bcodmo_name String fluorescence
attribute Fm description String Maximum fluorescence
attribute Fm long_name String FM
attribute Fm nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/ (external link)
attribute Fm units String unitless
variable Yield   float  
attribute Yield _FillValue float NaN
attribute Yield actual_range float 0.0, 1.0
attribute Yield bcodmo_name String Fv2Fm
attribute Yield description String Effective (or Optimal if dark-adapted) quantum yield. Calculated as Yield = (Fm - F)/Fm
attribute Yield long_name String Yield
attribute Yield units String unitless
variable rETR   float  
attribute rETR _FillValue float NaN
attribute rETR actual_range float 0.0, 1008.5
attribute rETR bcodmo_name String unknown
attribute rETR description String Relative Electron Transport Rate
attribute rETR long_name String R ETR
attribute rETR units String unitless
variable qP   float  
attribute qP _FillValue float NaN
attribute qP actual_range float 0.0, 4.0
attribute qP bcodmo_name String unknown
attribute qP description String Photochemical quenching
attribute qP long_name String Q P
attribute qP units String unitless
variable qN   float  
attribute qN _FillValue float NaN
attribute qN actual_range float 0.0, 11.0
attribute qN bcodmo_name String unknown
attribute qN description String Non-photochemical quenching expressed as a fraction of difference between Fm and minimum F
attribute qN long_name String Q N
attribute qN units String unitless
variable NPQ   float  
attribute NPQ _FillValue float NaN
attribute NPQ actual_range float 0.0, 28.797
attribute NPQ bcodmo_name String unknown
attribute NPQ description String Non-photochemical quenching normalized to Fm measured in the light
attribute NPQ long_name String NPQ
attribute NPQ units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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