ERDDAP > info > bcodmo_dataset_738772

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[coccolithophore-associated biopolymers for radionuclides] - Coccolithophore-associated organic biopolymers for fractionating particle-reactive radionuclides (234Th, 233Pa, 210Pb, 210Po, and 7Be) (Biopolymers as carrier phases for selected natural radionuclides (of Th, Pa, Pb, Po, Be) in diatoms and coccolithophores)		I   M	background			BCO-DMO	bcodmo_dataset_738772

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	The seawater (< 1 kDa) was enriched with f/2 nutrients, trace metals and vitamins, and autoclaved in pre-combusted and seawater-preconditioned clear glassware. Then, ~50 Bq of each gamma emitting radionuclide, including 234Th, 233Pa, 210Pb and 7Be, was added. Since 210Po emits no gamma radiation, 210Po was added separately into the seawater. After checking the pH of each radiolabeled medium to be 8.0, 2 mL of laboratory axenic Emiliania huxleyi (CCMP 371) was added to 100 mL of media and incubated at a temperature of 19\u00b11\u00baC with a light:dark cycle of 14 h:10 h under an irradiation condition of 100 \u00b5mol-quanta/m2/s. Non-attached exopolymeric substances (NAEPS) and exopolymeric substances attached on the coccolithophore cellular surface (AEPS) were extracted followed the procedures described in Chuang et al. (2015) and Xu et al. (2011). In brief, laboratory cultures were centrifuged at 3000 \u00d7g for 30 min, and then the supernatant for the NAEPS fraction was filtered, followed by the concentration and extensive desalting of supernatant against nanopure water (18.2 \u03a9) with 3 kDa Microsep centrifugal filter tubes (Milipore). For AEPS extraction, the resultant pellet from the centrifugation was resuspended by 50 mL 3% NaCl solution and stirred gently overnight at 4\u00baC. Lastly, the solution was centrifuged, and the supernatant containing the AEPS was then filtered before further desalting via the 3 kDa ultrafiltration centrifugation tubes. The pellet from the previous step was thus further digested in the 0.44 M HAc + 0.1 M NaCl solution at 4\u00baC for 8 h. After the digestion, the mixed solution was centrifuged and filtered, followed by ultrafiltration of the supernatant with 3 kDa Microsep centrifugal filter tubes. The retentate (> 3 kDa) was defined as coccosphere-associated biopolymers. The permeate (<3 kDa), defined as the fraction of digested biogenic calcite. Cells remaining from the last step was further heated in 20 mL of 1% SDS containing 10 mM Tris solution (pH 6.8) at 95 \u00baC for 1 h. The supernatant was also collected through centrifugation and filtration, followed by desalting and concentration with 3 kDa Microsep centrifugal filter tubes. Subsequently, the pellet was further digested by 0.04 M NH2OH HCl + 4.35 M HAc mixture at 96 \u00baC for 6 h, with occasional agitation to obtain the intracellular Fe-Mn associated metabolitic biopolymer. The sum of these two fractions represents the intracellular biopolymers after cell breakage. Subsamples were taken from the concentrated biopolymers for the analysis of protein, total carbohydrate (TCHO) and uronic acid (URA), respectively. In brief, the protein abundance was measured through a modified Lowry protein assay, using bovine serum albumin (BSA) as the standard. For the concentrations of TCHO, samples were hydrolyzed by 0.09 M HCl (final concentration) at 150\u00baC for 1 h. After neutralization with NaOH solution, the hydrolysate was measured by the 2,4,6-tripyridyl-triazine (TPTZ) method (Hung et al., 2001), with glucose as the standard. URA concentrations were determined by the metahydroxyphenyl method using glucuronic acid as the standard (Hung and Santschi, 2001).\u00a0 All the solutions from the different extraction steps, including the >3 kDa biopolymer fractions and the permeate (< 3 kDa), were counted for the activity concentrations of 234Th, 233Pa, 210Pb and 7Be by a Canberra ultrahigh purity germanium well gamma detector. In addition, the 210Po activity in different separately incubated fractions was determined by Beckman Model 8100 Liquid Scintillation Counter.
attribute	NC_GLOBAL	awards_0_award_nid	String	735995
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1356453
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1356453
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Henrietta N Edmonds
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	51517
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Coccolithophore-associated organic biopolymers for fractionating particle-reactive radionuclides (234Th, 233Pa, 210Pb, 210Po, and 7Be) PI: Peter H. Santschi Version 2018-05-15 Notes: "n.d." denotes lower than detection limit "-" denotes data not available NAEPS = Non-attached exopolymeric substances AEPS = Attached exopolymeric substances
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2018-06-18T18:14:36Z
attribute	NC_GLOBAL	date_modified	String	2019-03-28T13:14:47Z
attribute	NC_GLOBAL	defaultDataQuery	String	&amp;time&lt;now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.738772.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/738772
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	LSC
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Beckman Model 8100 Liquid Scintillation Counter
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	738804
attribute	NC_GLOBAL	instruments_0_description	String	Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used the quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB21/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Liquid Scintillation Counter
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	624
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Beckman Model 8100 Liquid Scintillation Counter
attribute	NC_GLOBAL	instruments_1_acronym	String	Spectrometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	UV-Visible spectrometer, BioTek Instruments Inc Model EPOCH
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	738781
attribute	NC_GLOBAL	instruments_1_description	String	A spectrometer is an optical instrument used to measure properties of light over a specific portion of the electromagnetic spectrum.
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0460/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Spectrometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	667
attribute	NC_GLOBAL	instruments_1_supplied_name	String	UV-Visible spectrometer
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Beckman Coulter Allegra X-12 centrifuge
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	738805
attribute	NC_GLOBAL	instruments_2_description	String	A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids.
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Centrifuge
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	629890
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Beckman Coulter Allegra X-12 centrifuge
attribute	NC_GLOBAL	instruments_3_dataset_instrument_description	String	Canberra ultrahigh purity germanium well gamma detector Model GCW3024
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	738780
attribute	NC_GLOBAL	instruments_3_description	String	Instruments measuring the relative levels of electromagnetic radiation of different wavelengths in the gamma-ray waveband.
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Gamma Ray Spectrometer
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	670659
attribute	NC_GLOBAL	instruments_3_supplied_name	String	Canberra ultrahigh purity germanium well gamma detector
attribute	NC_GLOBAL	keywords	String	activity, amount, bco, bco-dmo, be7, Be7_Activity, biological, biopolymer, Biopolymer_Fraction, chemical, data, dataset, dmo, erddap, fraction, management, oceanography, office, pa233, Pa233_Activity, pb210, Pb210_Activity, pcnt, po210, Po210_Activity, preliminary, protein, Protein_Amount, Protein_C_TCHO_C, tcho, TCHO_Amount, th234, Th234_Activity, ura, URA_Amount, URA_pcnt_TCHO
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/738772/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/738772
attribute	NC_GLOBAL	param_mapping	String	{'738772': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/738772/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Texas A&M, Galveston
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	TAMUG
attribute	NC_GLOBAL	people_0_person_name	String	Peter Santschi
attribute	NC_GLOBAL	people_0_person_nid	String	735998
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Texas A&M, Galveston
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	TAMUG
attribute	NC_GLOBAL	people_1_person_name	String	Antonietta Quigg
attribute	NC_GLOBAL	people_1_person_nid	String	736000
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Texas A&M, Galveston
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	TAMUG
attribute	NC_GLOBAL	people_2_person_name	String	Kathleen Schwehr
attribute	NC_GLOBAL	people_2_person_nid	String	736002
attribute	NC_GLOBAL	people_2_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	Texas A&M, Galveston
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	TAMUG
attribute	NC_GLOBAL	people_3_person_name	String	Chen Xu
attribute	NC_GLOBAL	people_3_person_nid	String	736004
attribute	NC_GLOBAL	people_3_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_3_role_type	String	originator
attribute	NC_GLOBAL	people_4_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_4_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_4_person_name	String	Mathew Biddle
attribute	NC_GLOBAL	people_4_person_nid	String	708682
attribute	NC_GLOBAL	people_4_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_4_role_type	String	related
attribute	NC_GLOBAL	project	String	Biopolymers for radionuclides
attribute	NC_GLOBAL	projects_0_acronym	String	Biopolymers for radionuclides
attribute	NC_GLOBAL	projects_0_description	String	NSF Award Abstract: Particle-associated natural radioisotopes are transported to the ocean floor mostly via silica and carbonate ballasted particles, allowing their use as tracers for particle transport. Th(IV), Pa (IV,V), Po(IV), Pb(II) and Be(II) radionuclides are important proxies in oceanographic investigations, used for tracing particle and colloid cycling, estimating export fluxes of particulate organic carbon, tracing air-sea exchange, paleoproductivity, and/or ocean circulation in paleoceanographic studies. Even though tracer approaches are considered routine, there are cases where data interpretation or validity has become controversial, largely due to uncertainties about inorganic proxies and organic carrier molecules. Recent studies showed that cleaned diatom frustules and pure silica particles, sorb natural radionuclides to a much lower extent (by 1-2 orders of magnitude) than whole diatom cells (with or without shells). Phytoplankton that build siliceous or calcareous shells, such as the diatoms and coccolithophores, are assembled via bio-mineralization processes using biopolymers as nanoscale templates. These templates could serve as possible carriers for radionuclides and stable metals. In this project, a research team at the Texas A & M University at Galveston hypothesize that radionuclide sorption is controlled by selective biopolymers that are associated with biogenic opal (diatoms), CaCO3 (coccolithophores) and the attached exopolymeric substances (EPS), rather than to pure mineral phase. To pursue this idea, the major objectives of their research will include separation, identification and molecular-level characterization of the individual biopolymers (e.g., polysaccharides, uronic acids, proteins, hydroquinones, hydroxamate siderophores, etc.) that are responsible for binding different radionuclides (Th, Pa, Pb, Po and Be) attached to cells or in the matrix of biogenic opal or CaCO3 as well as attached EPS mixture, in laboratory grown diatom and coccolithophore cultures. Laboratory-scale radiolabeling experiments will be conducted, and different separation techniques and characterization techniques will be applied. Intellectual Merit : It is expected that this study will help elucidate the molecular basis of the templated growth of diatoms and coccoliths, EPS and their role in scavenging natural radionuclides in the ocean, and help resolve debates on the oceanographic tracer applications of different natural radioisotopes (230,234Th, 231Pa, 210Po, 210Pb and 7,10Be). The proposed interdisciplinary research project will require instrumental approaches for molecular-level characterization of these radionuclides associated carrier molecules. Broader Impacts: The results of this study will be relevant for understanding biologically mediated ocean scavenging of radionuclides by diatoms and coccoliths which is important for carbon cycling in the ocean, and will contribute to improved interpretation of data obtained by field studies especially through the GEOTRACES program. This new program will enhance training programs at TAMUG for postdocs, graduate and undergraduate students. Lastly, results will be integrated in college courses and out-reach activities at Texas A&M University, including NSF-REU, Sea Camp, Elder Hostel and exhibits at the local science fair and interaction with its after-school program engaging Grade 9-12 students from groups traditionally underrepresented.
attribute	NC_GLOBAL	projects_0_end_date	String	2018-02
attribute	NC_GLOBAL	projects_0_name	String	Biopolymers as carrier phases for selected natural radionuclides (of Th, Pa, Pb, Po, Be) in diatoms and coccolithophores
attribute	NC_GLOBAL	projects_0_project_nid	String	735996
attribute	NC_GLOBAL	projects_0_start_date	String	2014-03
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Laboratory incubation experiments using the coccolithophore Emiliania huxleyi were conducted in the presence of 234Th, 233Pa, 210Pb, 210Po, and 7Be to differentiate radionuclide uptake to the CaCO3 coccosphere from coccolithophore-associated biopolymers.
attribute	NC_GLOBAL	title	String	[coccolithophore-associated biopolymers for radionuclides] - Coccolithophore-associated organic biopolymers for fractionating particle-reactive radionuclides (234Th, 233Pa, 210Pb, 210Po, and 7Be) (Biopolymers as carrier phases for selected natural radionuclides (of Th, Pa, Pb, Po, Be) in diatoms and coccolithophores)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Biopolymer_Fraction		String
attribute	Biopolymer_Fraction	bcodmo_name	String	sample_descrip
attribute	Biopolymer_Fraction	description	String	biopolymer fraction of E. huxleyi cells
attribute	Biopolymer_Fraction	long_name	String	Biopolymer Fraction
attribute	Biopolymer_Fraction	units	String	unitless
variable	Th234_Activity		String
attribute	Th234_Activity	bcodmo_name	String	radioactive_isotope_conc
attribute	Th234_Activity	description	String	percentage of 234Th activity (%)
attribute	Th234_Activity	long_name	String	Th234 Activity
attribute	Th234_Activity	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/WRAD/
attribute	Th234_Activity	units	String	unitless
variable	Pa233_Activity		String
attribute	Pa233_Activity	bcodmo_name	String	radioactive_isotope_conc
attribute	Pa233_Activity	description	String	percentage of 233Pa activity (%)
attribute	Pa233_Activity	long_name	String	Pa233 Activity
attribute	Pa233_Activity	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/WRAD/
attribute	Pa233_Activity	units	String	unitless
variable	Pb210_Activity		String
attribute	Pb210_Activity	bcodmo_name	String	radioactive_isotope_conc
attribute	Pb210_Activity	description	String	percentage of 210Pb activity (%)
attribute	Pb210_Activity	long_name	String	Pb210 Activity
attribute	Pb210_Activity	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/WRAD/
attribute	Pb210_Activity	units	String	unitless
variable	Po210_Activity		String
attribute	Po210_Activity	bcodmo_name	String	radioactive_isotope_conc
attribute	Po210_Activity	description	String	percentage of 210Po activity (%)
attribute	Po210_Activity	long_name	String	Po210 Activity
attribute	Po210_Activity	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/WRAD/
attribute	Po210_Activity	units	String	unitless
variable	Be7_Activity		String
attribute	Be7_Activity	bcodmo_name	String	radioactive_isotope_conc
attribute	Be7_Activity	description	String	percentage of 7Be activity (%)
attribute	Be7_Activity	long_name	String	Be7 Activity
attribute	Be7_Activity	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/WRAD/
attribute	Be7_Activity	units	String	unitless
variable	Protein_Amount		String
attribute	Protein_Amount	bcodmo_name	String	proteins
attribute	Protein_Amount	description	String	amount of organic protein component
attribute	Protein_Amount	long_name	String	Protein Amount
attribute	Protein_Amount	units	String	microMole Carbon (uM-C)
variable	TCHO_Amount		float
attribute	TCHO_Amount	_FillValue	float	NaN
attribute	TCHO_Amount	actual_range	float	1.0, 25.7
attribute	TCHO_Amount	bcodmo_name	String	carbohydrates
attribute	TCHO_Amount	description	String	amount of organic total carbohydrate component
attribute	TCHO_Amount	long_name	String	TCHO Amount
attribute	TCHO_Amount	units	String	microMole Carbon (uM-C)
variable	URA_Amount		float
attribute	URA_Amount	_FillValue	float	NaN
attribute	URA_Amount	actual_range	float	0.2, 38.2
attribute	URA_Amount	bcodmo_name	String	unknown
attribute	URA_Amount	description	String	amount of organic Uronic acid component
attribute	URA_Amount	long_name	String	URA Amount
attribute	URA_Amount	units	String	microMole Carbon (uM-C)
variable	Protein_C_TCHO_C		float
attribute	Protein_C_TCHO_C	_FillValue	float	NaN
attribute	Protein_C_TCHO_C	actual_range	float	0.0, 6.2
attribute	Protein_C_TCHO_C	bcodmo_name	String	unknown
attribute	Protein_C_TCHO_C	description	String	ratio of proteins to total carbohydrates
attribute	Protein_C_TCHO_C	long_name	String	Protein C TCHO C
attribute	Protein_C_TCHO_C	units	String	unitless
variable	URA_pcnt_TCHO		byte
attribute	URA_pcnt_TCHO	_FillValue	byte	127
attribute	URA_pcnt_TCHO	actual_range	byte	13, 100
attribute	URA_pcnt_TCHO	bcodmo_name	String	unknown
attribute	URA_pcnt_TCHO	description	String	the percentage of the uronic acid in the bulk total carbohydrates pool
attribute	URA_pcnt_TCHO	long_name	String	URA Pcnt TCHO
attribute	URA_pcnt_TCHO	units	String	unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.