ERDDAP > info > bcodmo_dataset_739973

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[AE1812 Alkaline phosphatase] - Alkaline phosphatase activities for in situ and incubation samples from RV/Atlantic Explorer cruise AE1812 cruise transect from Bermuda to Rhode Island in May 2018. (Collaborative Research: Defining the biogeochemical drivers of diatom physiological ecology in the North Atlantic)		F   I   M	background			BCO-DMO	bcodmo_dataset_739973

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute	NC_GLOBAL	acquisition_description	String	For APA analysis, triplicate biological samples (250 mL) from in situ and incubation samples were filtered onto 47-mm polycarbonate membranes (0.2 \u03bcm). Stored at \u221220\u00b0C until analysis. APA was assayed after Dyhrman and Ruttenberg (2006) using the fluorogenic phosphatase substrate 6,8-difluoro-4-methylumbelliferyl phosphate. Values were normalized to both volume and chl a. Reagents/Abs/Em used: D-6567 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP): - Storage upon receipt: \u2264 20\u00b0C; Desiccate - Abs/Em = 358/455 - Molecular Formula: C10H7F2O6P - Molecular Weight: 292.1 - CAS Name/Number: 2H-1-Benzopyran-2-one, 6,8-difluoro-4-methyl-7-(phosphonooxy)-/ 214491-43-7 D-6566 6,8-difluoro-7-Hydroxy-4-Methylcoumarin (DiFMU) - Reference Standard: - Storage upon receipt: Room temp.; protect from light - Molecular Formula: C10H6F2O3 - Molecular Weight: 212.15 - CAS Name/Number: 2H-1-Benzopyran-2-one, 6,8-difluoro-7-hydroxy-4-methyl-/ 215868-23-8 Incubation key: Control = no addition of nutrients or deep water DSW = deep seawater addition (added 20% deep seawater (700 m)) +P = Added phosphate only (0.5 \u00b5M final for incubations 1 and 2, 1 \u00b5M final for incubation 3) +N = Added nitrate only (6 \u00b5M final for incubations 1 and 2, 12 \u00b5M final for incubation 3) phi_P = All but P added (N, Si, Fe, B12) \u00a0phi_N = All but N added (P, Si, Fe, B12) -1, -2, -3 = biological replicates In situ key: IS = in situ -1, -2, -3 = biological replicates Lost = sample was lost
attribute	NC_GLOBAL	awards_0_award_nid	String	704773
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1558506
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1558506
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	AE1812 Alkaline phosphatase activity in situs and incubation samples from AE1812 cruise transect from Bermuda to Rhode Island in May 2018 PI: S. Dyhrman (LDEO) version: 2018-07-17
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	dataset_current_state	String	Final and no updates
attribute	NC_GLOBAL	date_created	String	2018-07-16T15:35:18Z
attribute	NC_GLOBAL	date_modified	String	2020-06-29T18:48:11Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.739973.1
attribute	NC_GLOBAL	Easternmost_Easting	double	-56.56
attribute	NC_GLOBAL	geospatial_lat_max	double	40.42
attribute	NC_GLOBAL	geospatial_lat_min	double	31.42
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	-56.56
attribute	NC_GLOBAL	geospatial_lon_min	double	-70.58
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/739973
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Samples were run on a Biotek Synergy fluorescent plate reader using black plates
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	739977
attribute	NC_GLOBAL	instruments_0_description	String	Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: https://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.
attribute	NC_GLOBAL	instruments_0_instrument_name	String	plate reader
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	528693
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Biotek Synergy fluorescent plate reader
attribute	NC_GLOBAL	keywords	String	apa, APA_nmolP_hr_liter, APA_nmolP_hr_ug_chla, bco, bco-dmo, biological, cast, chemical, chla, chlorophyll, chlorophyll-a, data, dataset, date, dmo, erddap, harvest, incubation, latitude, liter, longitude, management, nmol, oceanography, office, preliminary, sample, station, time
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/739973/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/739973
attribute	NC_GLOBAL	Northernmost_Northing	double	40.42
attribute	NC_GLOBAL	param_mapping	String	{'739973': {'lat': 'flag - latitude', 'lon': 'flag - longitude'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/739973/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Lamont-Doherty Earth Observatory
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	LDEO
attribute	NC_GLOBAL	people_0_person_name	String	Sonya T. Dyhrman
attribute	NC_GLOBAL	people_0_person_nid	String	51101
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	University of Rhode Island
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	URI-GSO
attribute	NC_GLOBAL	people_1_person_name	String	Bethany D. Jenkins
attribute	NC_GLOBAL	people_1_person_nid	String	558172
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	University of Rhode Island
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	URI-GSO
attribute	NC_GLOBAL	people_2_person_name	String	Tatiana Rynearson
attribute	NC_GLOBAL	people_2_person_nid	String	511706
attribute	NC_GLOBAL	people_2_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_3_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_3_person_nid	String	50396
attribute	NC_GLOBAL	people_3_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	project	String	North Atlantic Diatoms
attribute	NC_GLOBAL	projects_0_acronym	String	North Atlantic Diatoms
attribute	NC_GLOBAL	projects_0_description	String	NSF abstract: About half of photosynthesis on earth is generated by marine phytoplankton, single celled organisms that drift with tides and currents. Within the phytoplankton, the diatoms conduct nearly half of this photosynthesis, exerting profound control over global carbon cycling. Despite their importance, there are surprisingly fundamental gaps in understanding how diatoms function in their natural environment, in part because methods to assess in situ physiology are lacking. This project focuses on the application of a powerful new approach, called Quantitative Metabolic Fingerprinting (QMF), to address this knowledge gap and examine species-specific physiology in the field. The project will provide transformative insights into how ocean geochemistry controls the distribution of diatoms, the metabolic responses of individual diatom species, and how metabolic potential is partitioned between diatom species, thus providing new insights into the structure and function of marine systems. The overarching goal is to examine how diatom species respond to changes in biogeochemistry across marine provinces, from the coast to the open ocean, by following shifts in diatom physiology using QMF. This research is critical to understand future changes in oceanic phytoplankton in response to climate and environmental change. Furthermore, activities on this project will include supporting a graduate student and postdoctoral fellow and delivering the Artistic Oceanographer Program (AOP) to diverse middle school age children and teachers in the NYC metropolitan area and to middle-school girls in the Girl Scouts of RI, reaching an anticipated 60 children and 30 teachers annually. The programs will foster multidisciplinary hands-on learning and will directly impact STEM education at a critical point in the pipeline by targeting diverse middle-school aged groups in both NY and RI. In laboratory studies with cultured isolates, there are profound differences among diatom species' responses to nutrient limitation. Thus, it is likely that different species contribute differently to nutrient uptake, carbon flux and burial. However, marine ecosystem models often rely on physiological attributes drawn from just one species and apply those attributes globally (e.g. coastal species used to model open ocean dynamics) or choose a single average value to represent all species across the world's oceans. In part, this is due to a relatively poor understanding of diatom physiological ecology and a limited tool set for assessing in situ diatom physiological ecology. This research project will address this specific challenge by explicitly tracking metabolic pathways, measuring their regulation and determining their taxonomic distribution in a suite of environmentally significant diatoms using a state of the art, species-specific approach. A research expedition is set in the North Atlantic, a system that plays a major role in carbon cycling. Starting with a New England coastal shelf site, samples will be collected from the coast where diatoms thrive, to the open ocean and a site of a long term ocean time series station (the Bermuda Atlantic Time Series) where diatom growth is muted by nutrient limitation. This research takes advantage of new ocean observatories initiative (OOI) and time series information. Through the research expedition and downstream laboratory experiments, the molecular pathways of nutrient metabolism and related gene expression in a suite of environmentally significant diatoms will be identified. Data will be combined to predict major limiting factors and potentially important substrates for diatoms across marine provinces. Importantly, this integrated approach takes advantage of new advances in molecular and bioinformatics tools to examine in situ physiological ecology at the species-specific level, a key knowledge gap in the field.
attribute	NC_GLOBAL	projects_0_end_date	String	2019-08
attribute	NC_GLOBAL	projects_0_geolocation	String	North Atlantic
attribute	NC_GLOBAL	projects_0_name	String	Collaborative Research: Defining the biogeochemical drivers of diatom physiological ecology in the North Atlantic
attribute	NC_GLOBAL	projects_0_project_nid	String	704768
attribute	NC_GLOBAL	projects_0_start_date	String	2016-09
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	31.42
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	This dataset reports alkaline phosphatase activities (APA) for 3 incubation runs and 33 in situ samples collected on RV/Atlantic Explorer cruise AE1812 in May 2018. The samples were collected between Bermuda and Rhode Island.
attribute	NC_GLOBAL	title	String	[AE1812 Alkaline phosphatase] - Alkaline phosphatase activities for in situ and incubation samples from RV/Atlantic Explorer cruise AE1812 cruise transect from Bermuda to Rhode Island in May 2018. (Collaborative Research: Defining the biogeochemical drivers of diatom physiological ecology in the North Atlantic)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	Westernmost_Easting	double	-70.58
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.5
variable	incubation		String
attribute	incubation	bcodmo_name	String	replicate
attribute	incubation	description	String	Incubation replicate or in situ sampling
attribute	incubation	long_name	String	Incubation
attribute	incubation	units	String	unitless
variable	sample		String
attribute	sample	bcodmo_name	String	sample
attribute	sample	description	String	Sample identifier
attribute	sample	long_name	String	Sample
attribute	sample	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	sample	units	String	unitless
variable	station		String
attribute	station	bcodmo_name	String	station
attribute	station	description	String	Station identification number
attribute	station	long_name	String	Station
attribute	station	units	String	unitless
variable	cast		String
attribute	cast	bcodmo_name	String	cast
attribute	cast	description	String	Cast number on cruise
attribute	cast	long_name	String	Cast
attribute	cast	units	String	unitless
variable	date_harvest		String
attribute	date_harvest	bcodmo_name	String	date
attribute	date_harvest	description	String	Day on which samples were filtered and stored; formatted as yyyy-mm-dd
attribute	date_harvest	long_name	String	Date Harvest
attribute	date_harvest	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	date_harvest	source_name	String	date_harvest
attribute	date_harvest	time_precision	String	1970-01-01
attribute	date_harvest	units	String	unitless
variable	APA_nmolP_hr_liter		String
attribute	APA_nmolP_hr_liter	bcodmo_name	String	unknown
attribute	APA_nmolP_hr_liter	description	String	Alkaline phosphatase activity; volume normalized
attribute	APA_nmolP_hr_liter	long_name	String	APA Nmol P Hr Liter
attribute	APA_nmolP_hr_liter	units	String	nanomol Phosphate/hour/liter [nmol P/h/L]
variable	APA_nmolP_hr_ug_chla		String
attribute	APA_nmolP_hr_ug_chla	bcodmo_name	String	unknown
attribute	APA_nmolP_hr_ug_chla	description	String	Alkaline phosphatase activity; chl a normalized
attribute	APA_nmolP_hr_ug_chla	long_name	String	APA Nmol P Hr Ug Chla
attribute	APA_nmolP_hr_ug_chla	units	String	nanomol Phosphate/hour/microgram chlorophyll-a [nmol P/h/µg Chl a]
variable	latitude		double
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	_FillValue	double	NaN
attribute	latitude	actual_range	double	31.42, 40.42
attribute	latitude	axis	String	Y
attribute	latitude	bcodmo_name	String	latitude
attribute	latitude	colorBarMaximum	double	90.0
attribute	latitude	colorBarMinimum	double	-90.0
attribute	latitude	description	String	latitude; north is positive
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LATX/
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	longitude		double
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	_FillValue	double	NaN
attribute	longitude	actual_range	double	-70.58, -56.56
attribute	longitude	axis	String	X
attribute	longitude	bcodmo_name	String	longitude
attribute	longitude	colorBarMaximum	double	180.0
attribute	longitude	colorBarMinimum	double	-180.0
attribute	longitude	description	String	longitude; east is positive
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LONX/
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.