ERDDAP > info > bcodmo_dataset_743224

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[SO248: Bulk MCAMUF hydrolysis rates] - Microbial enzyme activities: glucosidase and peptidase activities of bulk seawater samples from the RV\Sonne cruise SO248 in the South and North Pacific, along 180 W, May, 2016 (Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?)		F   I   M	background			BCO-DMO	bcodmo_dataset_743224

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson,.odvTxt
attribute	NC_GLOBAL	acquisition_description	String	Water was collected via Niskin bottles mounted on a rosette, equipped with a CTD. Two substrates, -glucose and -glucose linked to a 4-methylumbelliferyl (MUF) fluorophore, were used to measure glucosidase activities. Five substrates linked to a 7-amido-4-methyl coumarin (MCA) fluorophore, one amino acid \u2013 leucine \u2013 and four oligopeptides \u2013 the chymotrypsin substrates alanine-alanine-phenylalanine (AAF) and alanine-alanine-proline-phenylalanine (AAPF), and the trypsin substrates glutamine-alanine-arginine (QAR) and phenylalanine-serine-arginine (FSR) \u2013 were used to measure exo- and endo- acting peptidase activities, respectively. Hydrolysis rates of the substrates were measured as an increase in fluorescence as the fluorophore was hydrolyzed from the substrate over time [as in Hoppe, 1993; Obayashi and Suzuki, 2005]. Incubations with the seven low molecular weight substrates were set up in a 96-well plate. For each substrate, triplicate wells were filled with a total volume of 200 L seawater for experimental incubations; triplicate wells were filled with 200 L autoclaved seawater for killed control incubations. Substrate was added at saturating concentrations. A saturation curve was determined with surface water from each station to determine saturating concentrations of substrate. The saturating concentration was identified as the lowest tested concentration of substrate at which additional substrate did not yield higher rates of hydrolysis. Fluorescence was measured over 24-48 hours incubation time with a plate reader (TECAN spectrafluor plus; 360 nm excitation, 460 emission), with timepoints taken every 4-6 hours. Hydrolysis rates were calculated from the rate of increase of fluorescence in the incubation over time relative to a set of standards of known concentration of fluorophore. Scripts to calculate hydrolysis rates and produce the figures shown here are available in the associated Github repository [Hoarfrost, 2017]. L = substrate to measure leucine aminopeptidase (L-leucine-7-amido-4 MCA) AAF = substrate to measure chymotrypsin activity: ala-ala-phe-MCA AAPF = substrate to measure chymotrypsin activity: N-succinyl-ala-ala-pro- phe-MCA QAR = substrate to measure trypsin activity: Boc-gln-ala-arg-MCA\u00a0\u00a0 FSR = substrate to measure trypsin activity: N-t-boc-phe-ser-arg-MCA\u00a0
attribute	NC_GLOBAL	awards_0_award_nid	String	712358
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1332881
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1332881
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Henrietta N Edmonds
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	51517
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Microbial Activities: Bulk MCAMUF RV/Sonne So248, South and North Pacific, along 180 W P.I.: C. Arnosti (UNC-CH) version: 2018-07-31 NOTE: Date/time and location data from CTD cast data (https://doi.pangaea.de/10.1594/PANGAEA.864673) were joined to original dataset
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	dataset_current_state	String	Final and no updates
attribute	NC_GLOBAL	date_created	String	2018-08-06T14:30:10Z
attribute	NC_GLOBAL	date_modified	String	2020-04-27T19:01:47Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.743224.1
attribute	NC_GLOBAL	Easternmost_Easting	double	180.0
attribute	NC_GLOBAL	geospatial_lat_max	double	58.9
attribute	NC_GLOBAL	geospatial_lat_min	double	-30.0008
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	180.0
attribute	NC_GLOBAL	geospatial_lon_min	double	-179.0
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	geospatial_vertical_max	double	4000.0
attribute	NC_GLOBAL	geospatial_vertical_min	double	20.0
attribute	NC_GLOBAL	geospatial_vertical_positive	String	down
attribute	NC_GLOBAL	geospatial_vertical_units	String	m
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/743224
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Niskin bottle
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	743230
attribute	NC_GLOBAL	instruments_0_description	String	A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Niskin bottle
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	413
attribute	NC_GLOBAL	instruments_1_acronym	String	CTD
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	743231
attribute	NC_GLOBAL	instruments_1_description	String	The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column and permits scientists observe the physical properties in real time via a conducting cable connecting the CTD to a deck unit and computer on the ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast. This instrument designation is used when specific make and model are not known.
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/130/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	CTD profiler
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	417
attribute	NC_GLOBAL	instruments_2_acronym	String	Fluorometer
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	743232
attribute	NC_GLOBAL	instruments_2_description	String	A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
attribute	NC_GLOBAL	instruments_2_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/113/
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Fluorometer
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	484
attribute	NC_GLOBAL	keywords	String	average, bco, bco-dmo, biological, cast, cast_no, chemical, data, dataset, date, depth, depth_m, depth_no, dev, dmo, elapsed, erddap, iso, latitude, longitude, management, oceanography, office, preliminary, profiler, rate, rep1, rep1_rate, rep2, rep2_rate, rep3, rep3_rate, salinity, salinity-temperature-depth, station, station_no, std, std_dev, substrate, temperature, time, time_elapsed_hr, timepoint
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/743224/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/743224
attribute	NC_GLOBAL	Northernmost_Northing	double	58.9
attribute	NC_GLOBAL	param_mapping	String	{'743224': {'Latitude': 'flag - latitude', 'depth_m': 'flag - depth', 'Longitude': 'flag - longitude', 'ISO_DateTime_UTC': 'flag - time'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/743224/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of North Carolina at Chapel Hill
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UNC-Chapel Hill
attribute	NC_GLOBAL	people_0_person_name	String	Carol Arnosti
attribute	NC_GLOBAL	people_0_person_nid	String	661940
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_1_person_nid	String	50396
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	Patterns of activities
attribute	NC_GLOBAL	projects_0_acronym	String	Patterns of activities
attribute	NC_GLOBAL	projects_0_description	String	NSF Award Abstract: Heterotrophic microbial communities are key players in the marine carbon cycle, transforming and respiring organic carbon, regenerating nutrients, and acting as the final filter in sediments through which organic matter passes before long-term burial. Microbially-driven carbon cycling in the ocean profoundly affects the global carbon cycle, but key factors determining rates and locations of organic matter remineralization are unclear. In this study, researchers from the University of North Carolina at Chapel Hill will investigate the ability of pelagic microbial communities to initiate the remineralization of polysaccharides and proteins, which together constitute a major pool of organic matter in the ocean. Results from this study will be predictive on a large scale regarding the nature of the microbial response to organic matter input, and will provide a mechanistic framework for interpreting organic matter reactivity in the ocean. Broader Impacts: This study will provide scientific training for undergraduate and graduate students from underrepresented groups. The project will also involve German colleagues, thus strengthening international scientific collaboration.
attribute	NC_GLOBAL	projects_0_end_date	String	2017-07
attribute	NC_GLOBAL	projects_0_geolocation	String	Atlantic Ocean, Arctic Ocean, Pacific Ocean, Greenland
attribute	NC_GLOBAL	projects_0_name	String	Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?
attribute	NC_GLOBAL	projects_0_project_nid	String	712359
attribute	NC_GLOBAL	projects_0_start_date	String	2013-08
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	-30.0008
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	This dataset includes MCAMUF (glucosidase and peptidase) hydrolysis rates to measure microbial enzyme activities in bulk (not filter-fractionated) seawater. Samples were collected on RV/Sonne cruise SO248 in May 2016. Links to archived CTD data are also provided.
attribute	NC_GLOBAL	time_coverage_end	String	2016-05-30T14:22Z
attribute	NC_GLOBAL	time_coverage_start	String	2016-05-02T16:51Z
attribute	NC_GLOBAL	title	String	[SO248: Bulk MCAMUF hydrolysis rates] - Microbial enzyme activities: glucosidase and peptidase activities of bulk seawater samples from the RV\Sonne cruise SO248 in the South and North Pacific, along 180 W, May, 2016 (Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	Westernmost_Easting	double	-179.0
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.5
variable	station_no		String
attribute	station_no	bcodmo_name	String	station
attribute	station_no	description	String	refers to station number for cruise
attribute	station_no	long_name	String	Station No
attribute	station_no	units	String	unitless
variable	cast_no		String
attribute	cast_no	bcodmo_name	String	cast
attribute	cast_no	description	String	cast number (refers to cast of CTD/Niskin bottles on cruise)
attribute	cast_no	long_name	String	Cast No
attribute	cast_no	units	String	unitless
variable	time		double
attribute	time	_CoordinateAxisType	String	Time
attribute	time	actual_range	double	1.46220786E9, 1.46461812E9
attribute	time	axis	String	T
attribute	time	bcodmo_name	String	ISO_DateTime_UTC
attribute	time	description	String	date and time in ISO format (yyyy-mm-ddTHH:MM:SS
attribute	time	ioos_category	String	Time
attribute	time	long_name	String	ISO Date Time UTC
attribute	time	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/
attribute	time	source_name	String	ISO_DateTime_UTC
attribute	time	standard_name	String	time
attribute	time	time_origin	String	01-JAN-1970 00:00:00
attribute	time	time_precision	String	1970-01-01T00:00Z
attribute	time	units	String	seconds since 1970-01-01T00:00:00Z
variable	latitude		double
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	_FillValue	double	NaN
attribute	latitude	actual_range	double	-30.0008, 58.9
attribute	latitude	axis	String	Y
attribute	latitude	bcodmo_name	String	latitude
attribute	latitude	colorBarMaximum	double	90.0
attribute	latitude	colorBarMinimum	double	-90.0
attribute	latitude	description	String	latitude; north is positive
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LATX/
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	longitude		double
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	_FillValue	double	NaN
attribute	longitude	actual_range	double	-179.0, 180.0
attribute	longitude	axis	String	X
attribute	longitude	bcodmo_name	String	longitude
attribute	longitude	colorBarMaximum	double	180.0
attribute	longitude	colorBarMinimum	double	-180.0
attribute	longitude	description	String	longitude; east is postivie
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LONX/
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east
variable	depth_no		String
attribute	depth_no	bcodmo_name	String	unknown
attribute	depth_no	description	String	sequence of depths sampled (1 is surface; higher numbers at greater depths)
attribute	depth_no	long_name	String	Depth
attribute	depth_no	standard_name	String	depth
attribute	depth_no	units	String	unitless
variable	depth		double
attribute	depth	_CoordinateAxisType	String	Height
attribute	depth	_CoordinateZisPositive	String	down
attribute	depth	_FillValue	double	NaN
attribute	depth	actual_range	double	20.0, 4000.0
attribute	depth	axis	String	Z
attribute	depth	bcodmo_name	String	depth
attribute	depth	colorBarMaximum	double	8000.0
attribute	depth	colorBarMinimum	double	-8000.0
attribute	depth	colorBarPalette	String	TopographyDepth
attribute	depth	description	String	actual depth at which water collected
attribute	depth	ioos_category	String	Location
attribute	depth	long_name	String	Depth
attribute	depth	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/DEPH/
attribute	depth	positive	String	down
attribute	depth	standard_name	String	depth
attribute	depth	units	String	m
variable	substrate		String
attribute	substrate	bcodmo_name	String	unknown
attribute	substrate	description	String	Substrates for measurement of enzymatic activities. ara:arabinogalactan: L = substrate to measure leucine aminopeptidase (L-leucine-7-amido-4 MCA) AAF = substrate to measure chymotrypsin activity: ala-ala-phe-MCA AAPF = substrate to measure chymotrypsin activity: N-succinyl-ala-ala-pro-phe-MCA QAR = substrate to measure trypsin activity: Boc-gln-ala-arg-MCA   FSR = substrate to measure trypsin activity: N-t-boc-phe-ser-arg-MCA
attribute	substrate	long_name	String	Substrate
attribute	substrate	units	String	unitless
variable	timepoint		byte
attribute	timepoint	_FillValue	byte	127
attribute	timepoint	actual_range	byte	1, 4
attribute	timepoint	bcodmo_name	String	time_point
attribute	timepoint	description	String	sampling point post-incubation
attribute	timepoint	long_name	String	Timepoint
attribute	timepoint	units	String	unitless
variable	time_elapsed_hr		float
attribute	time_elapsed_hr	_FillValue	float	NaN
attribute	time_elapsed_hr	actual_range	float	11.1, 74.8
attribute	time_elapsed_hr	bcodmo_name	String	time_elapsed
attribute	time_elapsed_hr	description	String	incubation time
attribute	time_elapsed_hr	long_name	String	Time Elapsed Hr
attribute	time_elapsed_hr	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/
attribute	time_elapsed_hr	units	String	hours
variable	rep1_rate		double
attribute	rep1_rate	_FillValue	double	NaN
attribute	rep1_rate	actual_range	double	0.0, 848.319433
attribute	rep1_rate	bcodmo_name	String	unknown
attribute	rep1_rate	description	String	replicate 1 hydrolysis rate
attribute	rep1_rate	long_name	String	Rep1 Rate
attribute	rep1_rate	units	String	nanomoles/liter/hour (nmol L-1 h-1)
variable	rep2_rate		double
attribute	rep2_rate	_FillValue	double	NaN
attribute	rep2_rate	actual_range	double	0.0, 959.721043
attribute	rep2_rate	bcodmo_name	String	unknown
attribute	rep2_rate	description	String	replicate 2 hydrolysis rate
attribute	rep2_rate	long_name	String	Rep2 Rate
attribute	rep2_rate	units	String	nanomoles/liter/hour (nmol L-1 h-1)
variable	rep3_rate		double
attribute	rep3_rate	_FillValue	double	NaN
attribute	rep3_rate	actual_range	double	0.0, 824.866787
attribute	rep3_rate	bcodmo_name	String	unknown
attribute	rep3_rate	description	String	replicate 3 hydrolysis rate
attribute	rep3_rate	long_name	String	Rep3 Rate
attribute	rep3_rate	units	String	nanomoles/liter/hour (nmol L-1 h-1)
variable	average		double
attribute	average	_FillValue	double	NaN
attribute	average	actual_range	double	0.0, 790.116399
attribute	average	bcodmo_name	String	unknown
attribute	average	description	String	average of hydrolysis rates
attribute	average	long_name	String	Average
attribute	average	units	String	nanomoles/liter/hour (nmol L-1 h-1)
variable	std_dev		double
attribute	std_dev	_FillValue	double	NaN
attribute	std_dev	actual_range	double	0.0, 519.337604
attribute	std_dev	bcodmo_name	String	unknown
attribute	std_dev	description	String	std deviation of hydrolysis rates
attribute	std_dev	long_name	String	Std Dev
attribute	std_dev	units	String	nanomoles/liter/hour (nmol L-1 h-1)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.