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Title Sum-
Institution Dataset ID
   set  data   graph     files  public Microbial enzyme activities: polysaccharide hydrolase activities of gravity filtered seawater
samples from the RV\\Sonne cruise SO248 in the South and North Pacific, along 180 W, May, 2016
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson,.odvTxt
attribute NC_GLOBAL acquisition_description String Water was collected via Niskin bottles mounted on a rosette, equipped with a

Experiments on (operationally defined) particles were carried out by gravity-
filtering water through 3 um pore size filters.\u00a0 1/12th sections of the 3
um pore-size filters were submerged in 15 mL artificial seawater; enzyme
activities were measured as described below.

The potential of the seawater microbial community associated with large
particles (> 3 micrometer pore size filter) to hydrolyze six high-molecular-
weight polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan,
laminarin, pullulan, and xylan) was investigated in surface and bottom water.
For each substrate, three\u00a0 15 mL falcon tubes were filled with autoclaved
artificial seawater and one 15 mL falcon tube was filled with autoclaved
seawater and a blank filter piece to serve as a killed control. Substrate was
added at 3.5 uM monomer-equivalent concentrations, except for fucoidan, which
was added at 5 uM concentrations (a higher concentration was necessary for
sufficient fluorescence signal). Two 15 mL falcon tubes \u2013 one with
seawater and one with autoclaved seawater \u2013 with no added substrate
served as blank controls. Incubations were stored in the dark at as close to
in situ temperature as possible. Subsamples of the incubations were collected
at time zero, and at six subsequent timepoints. At each timepoint, 2 mL of
seawater was collected from the 50 mL falcon tube using a sterile syringe,
filtered through a 0.2 um pore size syringe filter, and stored frozen until

The hydrolysis of high molecular weight substrate to lower molecular weight
hydrolysis products was measured using gel permeation chromatography with
fluorescence detection, after the method of Arnosti [1996, 2003]. In short,
the subsample was injected onto a series of columns consisting of a 21 cm
column of G50 and a 19 cm column of G75 Sephadex gel. The fluorescence of the
column effluent was measured at excitation and emission wavelengths of 490 and
530 nm, respectively. Hydrolysis rates were calculated from the change in
molecular weight distribution of the substrate over time, as described in
detail in Arnosti [2003].

ara = arabinogalactan
chn = chondroitin sulfate
fuc = fucoidan
lam = laminarin\u00a0
pul = pullulan
xyl = xylan
attribute NC_GLOBAL awards_0_award_nid String 712358
attribute NC_GLOBAL awards_0_award_number String OCE-1332881
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1332881 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Henrietta N Edmonds
attribute NC_GLOBAL awards_0_program_manager_nid String 51517
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Microbial Activities: GF FLA
RV/Sonne So248, South and North Pacific, along 180 W
P.I.: C. Arnosti (UNC-CH)
version: 2018-07-31
NOTE: Date/time and location data from CTD cast data (https://doi.pangaea.de/10.1594/PANGAEA.864673) were joined to original dataset
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL dataset_current_state String Final and no updates
attribute NC_GLOBAL date_created String 2018-08-06T16:35:36Z
attribute NC_GLOBAL date_modified String 2020-04-27T18:12:06Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.743274.1
attribute NC_GLOBAL Easternmost_Easting double 180.0
attribute NC_GLOBAL geospatial_lat_max double 56.9999
attribute NC_GLOBAL geospatial_lat_min double -30.0008
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double 180.0
attribute NC_GLOBAL geospatial_lon_min double -176.4753
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 1000.0
attribute NC_GLOBAL geospatial_vertical_min double 60.0
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/743274 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Niskin bottle
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 743280
attribute NC_GLOBAL instruments_0_description String A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Niskin bottle
attribute NC_GLOBAL instruments_0_instrument_nid String 413
attribute NC_GLOBAL instruments_1_acronym String CTD
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 743281
attribute NC_GLOBAL instruments_1_description String The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column and permits scientists observe the physical properties in real time via a conducting cable connecting the CTD to a deck unit and computer on the ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast. This instrument designation is used when specific make and model are not known.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/130/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String CTD profiler
attribute NC_GLOBAL instruments_1_instrument_nid String 417
attribute NC_GLOBAL instruments_2_acronym String Fluorometer
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 743282
attribute NC_GLOBAL instruments_2_description String A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/113/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Fluorometer
attribute NC_GLOBAL instruments_2_instrument_nid String 484
attribute NC_GLOBAL instruments_3_acronym String GPC
attribute NC_GLOBAL instruments_3_dataset_instrument_nid String 743283
attribute NC_GLOBAL instruments_3_description String Instruments that separate components in aqueous or organic solution based on molecular size generally for molecular weight determination. Gel permeation chromatography (GPC) is a type of size exclusion chromatography (SEC), that separates analytes on the basis of size.
attribute NC_GLOBAL instruments_3_instrument_name String Gel Permeation Chromatograph
attribute NC_GLOBAL instruments_3_instrument_nid String 669469
attribute NC_GLOBAL keywords String average, bco, bco-dmo, biological, cast, cast_no, chemical, data, dataset, date, depth, depth_m, depth_no, dev, dmo, elapsed, erddap, filter, filter_um, iso, latitude, longitude, management, oceanography, office, preliminary, profiler, rate, rep1, rep1_rate, rep2, rep2_rate, salinity, salinity-temperature-depth, station, station_no, std, std_dev, substrate, temperature, time, time_elapsed_hr, timepoint
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/743274/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/743274 (external link)
attribute NC_GLOBAL Northernmost_Northing double 56.9999
attribute NC_GLOBAL param_mapping String {'743274': {'Latitude': 'flag - latitude', 'depth_m': 'flag - depth', 'Longitude': 'flag - longitude', 'ISO_DateTime_UTC': 'flag - time'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/743274/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of North Carolina at Chapel Hill
attribute NC_GLOBAL people_0_affiliation_acronym String UNC-Chapel Hill
attribute NC_GLOBAL people_0_person_name String Carol Arnosti
attribute NC_GLOBAL people_0_person_nid String 661940
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Nancy Copley
attribute NC_GLOBAL people_1_person_nid String 50396
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String Patterns of activities
attribute NC_GLOBAL projects_0_acronym String Patterns of activities
attribute NC_GLOBAL projects_0_description String NSF Award Abstract:
Heterotrophic microbial communities are key players in the marine carbon cycle, transforming and respiring organic carbon, regenerating nutrients, and acting as the final filter in sediments through which organic matter passes before long-term burial. Microbially-driven carbon cycling in the ocean profoundly affects the global carbon cycle, but key factors determining rates and locations of organic matter remineralization are unclear. In this study, researchers from the University of North Carolina at Chapel Hill will investigate the ability of pelagic microbial communities to initiate the remineralization of polysaccharides and proteins, which together constitute a major pool of organic matter in the ocean. Results from this study will be predictive on a large scale regarding the nature of the microbial response to organic matter input, and will provide a mechanistic framework for interpreting organic matter reactivity in the ocean.
Broader Impacts: This study will provide scientific training for undergraduate and graduate students from underrepresented groups. The project will also involve German colleagues, thus strengthening international scientific collaboration.
attribute NC_GLOBAL projects_0_end_date String 2017-07
attribute NC_GLOBAL projects_0_geolocation String Atlantic Ocean, Arctic Ocean, Pacific Ocean, Greenland
attribute NC_GLOBAL projects_0_name String Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean?
attribute NC_GLOBAL projects_0_project_nid String 712359
attribute NC_GLOBAL projects_0_start_date String 2013-08
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double -30.0008
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String filter_um
attribute NC_GLOBAL summary String This dataset includes polysaccharide hydrolysis rates measured on particles collected from gravity-filtered seawater. Gravity filtered samples were collected on RV/Sonne cruise SO248 in May 2016. Links to archived CTD data are also provided.
attribute NC_GLOBAL time_coverage_end String 2016-05-29T21:43Z
attribute NC_GLOBAL time_coverage_start String 2016-05-02T16:51Z
attribute NC_GLOBAL title String Microbial enzyme activities: polysaccharide hydrolase activities of gravity filtered seawater samples from the RV\\Sonne cruise SO248 in the South and North Pacific, along 180 W, May, 2016
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -176.4753
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5
variable station_no   String  
attribute station_no bcodmo_name String station
attribute station_no description String refers to station number for cruise
attribute station_no long_name String Station No
attribute station_no units String unitless
variable cast_no   String  
attribute cast_no bcodmo_name String cast
attribute cast_no description String cast number (refers to cast of CTD/Niskin bottles on cruise)
attribute cast_no long_name String Cast No
attribute cast_no units String unitless
variable time   double  
attribute time _CoordinateAxisType String Time
attribute time actual_range double 1.46220786E9, 1.46455818E9
attribute time axis String T
attribute time bcodmo_name String ISO_DateTime_UTC
attribute time description String date and time in ISO format (yyyy-mm-ddTHH:MM:SS
attribute time ioos_category String Time
attribute time long_name String ISO Date Time UTC
attribute time nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/ (external link)
attribute time source_name String ISO_DateTime_UTC
attribute time standard_name String time
attribute time time_origin String 01-JAN-1970 00:00:00
attribute time time_precision String 1970-01-01T00:00Z
attribute time units String seconds since 1970-01-01T00:00:00Z
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double -30.0008, 56.9999
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String latitude; north is positive
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -176.4753, 180.0
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String longitude; east is postivie
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable depth_no   String  
attribute depth_no bcodmo_name String unknown
attribute depth_no description String sequence of depths sampled (1 is surface; higher numbers at greater depths)
attribute depth_no long_name String Depth
attribute depth_no standard_name String depth
attribute depth_no units String unitless
variable depth   double  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth _FillValue double NaN
attribute depth actual_range double 60.0, 1000.0
attribute depth axis String Z
attribute depth bcodmo_name String depth
attribute depth colorBarMaximum double 8000.0
attribute depth colorBarMinimum double -8000.0
attribute depth colorBarPalette String TopographyDepth
attribute depth description String actual depth at which water collected
attribute depth ioos_category String Location
attribute depth long_name String Depth
attribute depth nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable substrate   String  
attribute substrate bcodmo_name String unknown
attribute substrate description String substrates for measurement of enzymatic activities. ara:arabinogalactan; chn:chondroitin sulfate; fuc:fucoidan; lam:laminarin ; pul:pullulan; xyl:xylan
attribute substrate long_name String Substrate
attribute substrate units String unitless
variable timepoint   String  
attribute timepoint bcodmo_name String time_point
attribute timepoint description String sampling point post-incubation
attribute timepoint long_name String Timepoint
attribute timepoint units String unitless
variable time_elapsed_hr   double  
attribute time_elapsed_hr _FillValue double NaN
attribute time_elapsed_hr actual_range double 0.0, 627.4166667
attribute time_elapsed_hr bcodmo_name String time_elapsed
attribute time_elapsed_hr description String incubation time
attribute time_elapsed_hr long_name String Time Elapsed Hr
attribute time_elapsed_hr nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/ (external link)
attribute time_elapsed_hr units String hours
variable rep1_rate   float  
attribute rep1_rate _FillValue float NaN
attribute rep1_rate actual_range float 0.0, 0.342319
attribute rep1_rate bcodmo_name String unknown
attribute rep1_rate description String replicate 1 hydrolysis rate
attribute rep1_rate long_name String Rep1 Rate
attribute rep1_rate units String nanomoles/liter/hour (nmol L-1 h-1)
variable rep2_rate   float  
attribute rep2_rate _FillValue float NaN
attribute rep2_rate actual_range float 0.0, 0.35826
attribute rep2_rate bcodmo_name String unknown
attribute rep2_rate description String replicate 2 hydrolysis rate
attribute rep2_rate long_name String Rep2 Rate
attribute rep2_rate units String nanomoles/liter/hour (nmol L-1 h-1)
variable average   float  
attribute average _FillValue float NaN
attribute average actual_range float 0.0, 0.348874
attribute average bcodmo_name String unknown
attribute average description String average of hydrolysis rates
attribute average long_name String Average
attribute average units String nanomoles/liter/hour (nmol L-1 h-1)
variable std_dev   float  
attribute std_dev _FillValue float NaN
attribute std_dev actual_range float 0.0, 0.145735
attribute std_dev bcodmo_name String unknown
attribute std_dev description String std deviation of hydrolysis rates
attribute std_dev long_name String Std Dev
attribute std_dev units String nanomoles/liter/hour (nmol L-1 h-1)
variable filter_um   byte  
attribute filter_um _FillValue byte 127
attribute filter_um actual_range byte 3, 3
attribute filter_um bcodmo_name String filter_size
attribute filter_um description String filter pore size used for gravity filtration
attribute filter_um long_name String Filter Um
attribute filter_um units String micrometers

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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