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Title Sum-
RSS Institution Dataset ID
     data        files  public Data describing bleaching in algae collected from Antarctica, Fiji, and California when
stressed by heat, cold, or low salinity.
   ?     I   M   background (external link) RSS BCO-DMO bcodmo_dataset_743763

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String These data are described in detail in Sotka et al., 2018. From May until
October 2015, we collected live thalli at each of 40 populations: 15 Japanese
(six source and nine nonsource), five western North American, 10 eastern North
American, and 10 European populations. We exposed a haphazardly chosen set of
approximately 20 thalli per population to extreme heat (1, 2, and 4 hr in
40\u00b0C), extreme cold (45, 75, and 105 min at \u221220\u00b0C), and low
salinities (8 days at 0, 5, and 10 ppt). For the heat tolerance assay, four
apices per thallus were individually placed into unsealed 2- ml
microcentrifuge tubes containing 175 \u03bcl of seawater. A control apex was
kept in a 15\u00b0C growth chamber, while three apices were placed in a
40\u00b0C water bath for 1, 2, and 4 hr. For the cold tolerance assay, four
apices were independently placed into sealed 250 \u03bcl PCR tubes containing
200 \u03bcl seawater. A control apex was kept in a 15\u00b0C incubator, while
three apices were placed in a \u221220\u00b0 C freezer for 45, 75, and 105
min. Apices were then placed into randomly assigned wells of 12- well tissue
culture plates (2.21- cm- diameter wells) filled with 4 ml of seawater and
incubated at 15\u00b0C for 8 days with a 12- hr light/dark cycle. For the
salinity tolerance assay, four apices were independently placed directly into
the 12- well tissue culture plates filled with 4 ml of seawater at a titration
of salinities diluted to 0, 5, and 10 ppt, with 30 ppt serving as a control.
Every 2 days for 8 days, we changed water and assessed thallus bleaching
(i.e., loss of photosynthetic pigments; Figure S2) as a proxy of mortality,
itself an important component of fitness. We generated a Bleaching Score (BS),
where \u201c1\u201d indicated no bleaching, \u201c2\u201d indicated partial
bleaching or color change to pink or white, and \u201c3\u201d indicated full
attribute NC_GLOBAL awards_0_award_nid String 505521
attribute NC_GLOBAL awards_0_award_number String OCE-1357386
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=1357386 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Dr Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Bleaching scores for non-native seaweed
E. Sotka & C. Murren, PIs
Version 20 August 2018
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.2d 13 Jun 2019
attribute NC_GLOBAL date_created String 2018-08-20T18:52:07Z
attribute NC_GLOBAL date_modified String 2019-06-10T16:00:52Z
attribute NC_GLOBAL defaultDataQuery String &time
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.743763.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/743763 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL keywords String assay, bco, bco-dmo, biological, bleach, bleach_score, chemical, data, dataset, day, dmo, erddap, ind, ind_type, management, oceanography, office, pop, preliminary, score, time, trt, type
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/743763 (external link)
attribute NC_GLOBAL param_mapping String {'743763': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/743763/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Grice Marine Laboratory - College of Charleston
attribute NC_GLOBAL people_0_affiliation_acronym String GML-CoC
attribute NC_GLOBAL people_0_person_name String Dr Erik Sotka
attribute NC_GLOBAL people_0_person_nid String 472705
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String College of Charleston
attribute NC_GLOBAL people_1_affiliation_acronym String CofC
attribute NC_GLOBAL people_1_person_name String Dr Courtney Murren
attribute NC_GLOBAL people_1_person_nid String 472706
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Grice Marine Laboratory - College of Charleston
attribute NC_GLOBAL people_2_affiliation_acronym String GML-CoC
attribute NC_GLOBAL people_2_person_name String Dr Allan Strand
attribute NC_GLOBAL people_2_person_nid String 472707
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Grice Marine Laboratory - College of Charleston
attribute NC_GLOBAL people_3_affiliation_acronym String GML-CoC
attribute NC_GLOBAL people_3_person_name String Dr Erik Sotka
attribute NC_GLOBAL people_3_person_nid String 472705
attribute NC_GLOBAL people_3_role String Contact
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL people_4_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_4_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_4_person_name String Hannah Ake
attribute NC_GLOBAL people_4_person_nid String 650173
attribute NC_GLOBAL people_4_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_4_role_type String related
attribute NC_GLOBAL project String Detecting genetic adaptation during marine invasions
attribute NC_GLOBAL projects_0_acronym String Genetic Adaptation Marine Inv
attribute NC_GLOBAL projects_0_description String Description from NSF award abstract:
Biological introductions, defined as the establishment of species in geographic regions outside the reach of their natural dispersal mechanisms, have dramatically increased in frequency during the 20th century and are now altering community structure and ecosystem function of virtually all marine habitats. To date, studies on marine invasions focus principally on demographic and ecological processes, and the importance of evolutionary processes has been rarely tested. This knowledge gap has implications for management policies, which attempt to prevent biological introductions and mitigate their impacts. The Asian seaweed Gracilaria vermiculophylla has been introduced to every continental margin in the Northern Hemisphere, and preliminary data indicate that non-native populations are both more resistant to heat stress and resistant to snail herbivory. The project will integrate population genetics, field survey and common-garden laboratory experiments to comprehensively address the role of rapid evolutionary adaptation in the invasion success of this seaweed. Specifically, the PIs will answer the following. What is the consequence of introductions on seaweed demography and mating systems? How many successful introductions have occurred in North America and Europe? Where did introduced propagules originate? Do native, native-source and non-native locations differ in environmental conditions? Do native, native-source and non-native populations differ in phenotype?
The intellectual merit of this project is based on three gaps in the literature. First, while biological invasions are widely recognized as a major component of global change, there are surprisingly few studies that compare native and non-native populations in their biology or ecology. Native and non-native populations will be surveyed in a similar manner, allowing assessment of differences in population dynamics, mating system, epifaunal and epiphytic communities, and the surrounding abiotic and biotic environment. Second, G. vermiculophylla exhibits a life cycle typical of other invasive species (including some benthic invertebrates), yet we still lack data on the effects of decoupling the haploid and diploid stages on genetic structure, and in turn, on the evolvability of their populations. Finally, this project will provide unequivocal evidence of an adaptive shift in a marine invasive. To our knowledge, such evolutionary change has been described previously for only a complex of marine copepod species. G. vermiculophylla will serve as a model for understanding evolution in other nuisance invasions, and perhaps lead to novel methods to counter future invasions or their spread.
attribute NC_GLOBAL projects_0_end_date String 2017-02
attribute NC_GLOBAL projects_0_geolocation String Estuaries of NW and NE Pacific; estuaries of NW and NE Atlantic
attribute NC_GLOBAL projects_0_name String Detecting genetic adaptation during marine invasions
attribute NC_GLOBAL projects_0_project_nid String 472708
attribute NC_GLOBAL projects_0_start_date String 2014-03
attribute NC_GLOBAL publisher_name String Hannah Ake
attribute NC_GLOBAL publisher_role String BCO-DMO Data Manager(s)
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v29
attribute NC_GLOBAL summary String Data describing bleaching in algae collected from Antarctica, Fiji, and California when stressed by heat, cold, or low salinity.
attribute NC_GLOBAL title String Data describing bleaching in algae collected from Antarctica, Fiji, and California when stressed by heat, cold, or low salinity.
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5-beta
variable ind_type   String  
attribute ind_type description String Common garden vs field collected samples
attribute ind_type ioos_category String Unknown
attribute ind_type long_name String Ind Type
attribute ind_type units String unitless
variable pop   String  
attribute pop description String Three letter abbreviation; see Sotka et al. 2018 Evolutionary Applications
attribute pop ioos_category String Unknown
attribute pop long_name String Pop
attribute pop units String unitless
variable assay   int  
attribute assay _FillValue int 2147483647
attribute assay actual_range int 1, 74223
attribute assay description String Type of assay; heat, cold, or salinity assay.
attribute assay ioos_category String Unknown
attribute assay long_name String Assay
attribute assay units String unitless
variable ind   String  
attribute ind description String Individual ID
attribute ind ioos_category String Unknown
attribute ind long_name String Ind
attribute ind units String unitless
variable trt   String  
attribute trt description String Levels of treatment
attribute trt ioos_category String Unknown
attribute trt long_name String TRT
attribute trt units String unitless
variable day   String  
attribute day description String Day 0, 2, 4, 6, 8 after treatment started
attribute day ioos_category String Time
attribute day long_name String Day
attribute day units String days
variable bleach_score   String  
attribute bleach_score description String 1=healthy; 2=partially healthy; 3=bleached
attribute bleach_score ioos_category String Unknown
attribute bleach_score long_name String Bleach Score
attribute bleach_score units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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