ERDDAP > info > bcodmo_dataset_743763

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Bleaching scores of introduced seaweed] - Data describing bleaching in algae collected from Antarctica, Fiji, and California when stressed by heat, cold, or low salinity. (Detecting genetic adaptation during marine invasions)		I   M	background			BCO-DMO	bcodmo_dataset_743763

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	These data are described in detail in Sotka et al., 2018. From May until October 2015, we collected live thalli at each of 40 populations: 15 Japanese (six source and nine nonsource), five western North American, 10 eastern North American, and 10 European populations. We exposed a haphazardly chosen set of approximately 20 thalli per population to extreme heat (1, 2, and 4 hr in 40\u00b0C), extreme cold (45, 75, and 105 min at \u221220\u00b0C), and low salinities (8 days at 0, 5, and 10 ppt). For the heat tolerance assay, four apices per thallus were individually placed into unsealed 2- ml microcentrifuge tubes containing 175 \u03bcl of seawater. A control apex was kept in a 15\u00b0C growth chamber, while three apices were placed in a 40\u00b0C water bath for 1, 2, and 4 hr. For the cold tolerance assay, four apices were independently placed into sealed 250 \u03bcl PCR tubes containing 200 \u03bcl seawater. A control apex was kept in a 15\u00b0C incubator, while three apices were placed in a \u221220\u00b0 C freezer for 45, 75, and 105 min. Apices were then placed into randomly assigned wells of 12- well tissue culture plates (2.21- cm- diameter wells) filled with 4 ml of seawater and incubated at 15\u00b0C for 8 days with a 12- hr light/dark cycle. For the salinity tolerance assay, four apices were independently placed directly into the 12- well tissue culture plates filled with 4 ml of seawater at a titration of salinities diluted to 0, 5, and 10 ppt, with 30 ppt serving as a control. Every 2 days for 8 days, we changed water and assessed thallus bleaching (i.e., loss of photosynthetic pigments; Figure S2) as a proxy of mortality, itself an important component of fitness. We generated a Bleaching Score (BS), where \u201c1\u201d indicated no bleaching, \u201c2\u201d indicated partial bleaching or color change to pink or white, and \u201c3\u201d indicated full bleaching.
attribute	NC_GLOBAL	awards_0_award_nid	String	505521
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1357386
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward?AWD_ID=1357386
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Michael E. Sieracki
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50446
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Bleaching scores for non-native seaweed E. Sotka & C. Murren, PIs Version 20 August 2018
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2018-08-20T18:52:07Z
attribute	NC_GLOBAL	date_modified	String	2019-06-10T16:00:52Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.743763.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/743763
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	keywords	String	assay, bco, bco-dmo, biological, bleach, bleach_score, chemical, data, dataset, day, dmo, erddap, ind, ind_type, management, oceanography, office, pop, preliminary, score, trt, type
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/743763/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/743763
attribute	NC_GLOBAL	param_mapping	String	{'743763': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/743763/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Grice Marine Laboratory - College of Charleston
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	GML-CoC
attribute	NC_GLOBAL	people_0_person_name	String	Erik Sotka
attribute	NC_GLOBAL	people_0_person_nid	String	472705
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	College of Charleston
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	CofC
attribute	NC_GLOBAL	people_1_person_name	String	Dr Courtney Murren
attribute	NC_GLOBAL	people_1_person_nid	String	472706
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Grice Marine Laboratory - College of Charleston
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	GML-CoC
attribute	NC_GLOBAL	people_2_person_name	String	Dr Allan Strand
attribute	NC_GLOBAL	people_2_person_nid	String	472707
attribute	NC_GLOBAL	people_2_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	Grice Marine Laboratory - College of Charleston
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	GML-CoC
attribute	NC_GLOBAL	people_3_person_name	String	Erik Sotka
attribute	NC_GLOBAL	people_3_person_nid	String	472705
attribute	NC_GLOBAL	people_3_role	String	Contact
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	people_4_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_4_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_4_person_name	String	Hannah Ake
attribute	NC_GLOBAL	people_4_person_nid	String	650173
attribute	NC_GLOBAL	people_4_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_4_role_type	String	related
attribute	NC_GLOBAL	project	String	Genetic Adaptation Marine Inv
attribute	NC_GLOBAL	projects_0_acronym	String	Genetic Adaptation Marine Inv
attribute	NC_GLOBAL	projects_0_description	String	Description from NSF award abstract: Biological introductions, defined as the establishment of species in geographic regions outside the reach of their natural dispersal mechanisms, have dramatically increased in frequency during the 20th century and are now altering community structure and ecosystem function of virtually all marine habitats. To date, studies on marine invasions focus principally on demographic and ecological processes, and the importance of evolutionary processes has been rarely tested. This knowledge gap has implications for management policies, which attempt to prevent biological introductions and mitigate their impacts. The Asian seaweed Gracilaria vermiculophylla has been introduced to every continental margin in the Northern Hemisphere, and preliminary data indicate that non-native populations are both more resistant to heat stress and resistant to snail herbivory. The project will integrate population genetics, field survey and common-garden laboratory experiments to comprehensively address the role of rapid evolutionary adaptation in the invasion success of this seaweed. Specifically, the PIs will answer the following. What is the consequence of introductions on seaweed demography and mating systems? How many successful introductions have occurred in North America and Europe? Where did introduced propagules originate? Do native, native-source and non-native locations differ in environmental conditions? Do native, native-source and non-native populations differ in phenotype? The intellectual merit of this project is based on three gaps in the literature. First, while biological invasions are widely recognized as a major component of global change, there are surprisingly few studies that compare native and non-native populations in their biology or ecology. Native and non-native populations will be surveyed in a similar manner, allowing assessment of differences in population dynamics, mating system, epifaunal and epiphytic communities, and the surrounding abiotic and biotic environment. Second, G. vermiculophylla exhibits a life cycle typical of other invasive species (including some benthic invertebrates), yet we still lack data on the effects of decoupling the haploid and diploid stages on genetic structure, and in turn, on the evolvability of their populations. Finally, this project will provide unequivocal evidence of an adaptive shift in a marine invasive. To our knowledge, such evolutionary change has been described previously for only a complex of marine copepod species. G. vermiculophylla will serve as a model for understanding evolution in other nuisance invasions, and perhaps lead to novel methods to counter future invasions or their spread.
attribute	NC_GLOBAL	projects_0_end_date	String	2017-02
attribute	NC_GLOBAL	projects_0_geolocation	String	Estuaries of NW and NE Pacific; estuaries of NW and NE Atlantic
attribute	NC_GLOBAL	projects_0_name	String	Detecting genetic adaptation during marine invasions
attribute	NC_GLOBAL	projects_0_project_nid	String	472708
attribute	NC_GLOBAL	projects_0_start_date	String	2014-03
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Data describing bleaching in algae collected from Antarctica, Fiji, and California when stressed by heat, cold, or low salinity.
attribute	NC_GLOBAL	title	String	[Bleaching scores of introduced seaweed] - Data describing bleaching in algae collected from Antarctica, Fiji, and California when stressed by heat, cold, or low salinity. (Detecting genetic adaptation during marine invasions)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	ind_type		String
attribute	ind_type	bcodmo_name	String	sample_descrip
attribute	ind_type	description	String	Common garden vs field collected samples
attribute	ind_type	long_name	String	Ind Type
attribute	ind_type	units	String	unitless
variable	pop		String
attribute	pop	bcodmo_name	String	sample_descrip
attribute	pop	description	String	Three letter abbreviation; see Sotka et al. 2018 Evolutionary Applications
attribute	pop	long_name	String	Pop
attribute	pop	units	String	unitless
variable	assay		int
attribute	assay	_FillValue	int	2147483647
attribute	assay	actual_range	int	1, 74223
attribute	assay	bcodmo_name	String	sample_descrip
attribute	assay	description	String	Type of assay; heat, cold, or salinity assay.
attribute	assay	long_name	String	Assay
attribute	assay	units	String	unitless
variable	ind		String
attribute	ind	bcodmo_name	String	sample
attribute	ind	description	String	Individual ID
attribute	ind	long_name	String	Ind
attribute	ind	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	ind	units	String	unitless
variable	trt		String
attribute	trt	bcodmo_name	String	treatment
attribute	trt	description	String	Levels of treatment
attribute	trt	long_name	String	TRT
attribute	trt	units	String	unitless
variable	day		String
attribute	day	bcodmo_name	String	day
attribute	day	description	String	Day 0, 2, 4, 6, 8 after treatment started
attribute	day	long_name	String	Day
attribute	day	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/DAYXXXXX/
attribute	day	units	String	days
variable	bleach_score		byte
attribute	bleach_score	_FillValue	byte	127
attribute	bleach_score	actual_range	byte	1, 3
attribute	bleach_score	bcodmo_name	String	sample_descrip
attribute	bleach_score	description	String	1=healthy; 2=partially healthy; 3=bleached
attribute	bleach_score	long_name	String	Bleach Score
attribute	bleach_score	units	String	unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.