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     data        files  public CSIA 15N AA data from phytoplankton, microzooplankton, and Calanus pacificus.    ?     I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_744468

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Hydrolysis, derivatization and analyses of samples for CSIA \u2013\u00a0 For
Exps. 3-4, samples (1-2 mg dry weight) for CSIA-AA were added to 1 ml 6 N
HPLC-grade HCl, then flushed with N2, capped with a Teflon-lined cap, and
hydrolyzed at 150\u00b0C for 70 min.\u00a0 The resulting hydrolysate was
evaporated to dryness under N2 at 55\u00b0C, redissolved in 1 ml 0.01 N HCl,
purified by filtration (0.45-\u03bcm hydrophilic filter), washed with 1 ml of
0.01 N HCl, and further purified using cation-exchange chromatography with a
5-cm resin column (Dowex 50WX8-400) in a glass Pasteur pipette.\u00a0 AAs were
eluted with 4 ml of 2 N NH4OH and evaporated to dryness under a stream of N2
at 80\u00b0C, then reacidified with 0.5 ml of 0.2 N HCl, flushed with N2,
heated to 110\u00b0C for 5 min and evaporated to dryness under N2 at
55\u00b0C.\u00a0 Hydrolyzed samples were esterified with 2 ml of 4:1
isopropanol:acetyl chloride, flushed with N2 and heated to 110\u00b0C for 60
min.\u00a0 After drying at 60\u00b0C under N2, the samples were acylated by
adding 1 ml of 3:1 methylene chloride:trifluoracetic anhydride (TFAA) and
heated to 100\u00b0C for 15 min.\u00a0 The derivatized AAs were further
purified by solvent extraction following (Ueda et al. 1989).\u00a0 The
acylated AA esters were evaporated at room temperature under N2 and
redissolved in 3 ml of 1:2 chloroform:P-buffer (KH2PO4 + Na2HPO4 in Milli-Q
water, pH 7).\u00a0 Vigorous shaking ensured that the derivitized AAs were
partitioned into chloroform while contaminants remained in the P-buffer.\u00a0
The solvents were separated by centrifugation (10 min at 600 g), the
chloroform was transferred to a clean vial, and the solvent extraction process
repeated.\u00a0 Finally, to ensure derivatization, the acylation step was
repeated.\u00a0 Samples were stored at \u201320\u00b0C in 3:1 methylene
chloride:TFAA for up to 2 weeks until analysis.\u00a0 AA derivatives were then
analyzed by isotope monitoring gas chromatography-mass spectrometry.\u00a0 We
used a Delta V Plus mass spectrometer interfaced with a Trace GC gas
chromatograph through a GC-C III combustion furnace (980\u00b0C), reduction
furnace (650\u00b0C), and liquid nitrogen cold trap.\u00a0 Internal reference
compounds (aminoadipic acid and norleucine) of known nitrogen isotopic
composition were co-injected with samples and used to normalize the measured
\u03b415N values of unknown AAs, and a suite of eight AAs with known isotopic
composition was analyzed every 3 injections for additional quality
control.\u00a0 At least three injections per sample were analyzed.\u00a0 For
Exps. 5 and 6, CSIA-AA samples were hydrolyzed in 6N HCl at 110\u00b0C for 20
h.\u00a0 The hydrolysate was evaporated to dryness at temperature under a
continuous stream of N2 gas, then esterified with 4:1 isotopropanol:acetyl
chloride at 110\u00b0C for 60 min, acylated in a 1:1 solution of methylene
chloride:trifluoracetic anhydride (DCM:TFAA) for 10 min at 110\u00b0C.\u00a0
Samples were stored at -20\u00b0C in 1:1 DCM:TFAA for up to 3 months before
isotope analysis using a Delta V Plus mass spectrometer (Thermo Scientific)
interfaced through a Conflo IV to a GC 1310 gas chromatograph coupled to a GC
Isolink combustion-reduction furnace (1000\u00b0C) and liquid nitrogen cold
trap.\u00a0 All samples were injected (splitless injector) onto a forte BPx5
capillary column (60 m x 0.32 mm x 1.0-\u03bcm film thickness) at an injector
temperature of 250\u00b0C with a constant helium flow rate of 1.4 ml
min\u20131.\u00a0 The column was initially held at 50\u00b0C for 2 min and
then increased to 125 \u00b0C at a rate of 15\u00b0C min-1.\u00a0 Once at
125\u00b0C, the temperature was increased at a rate of 3\u00b0C min-1 to
160\u00b0C and then to 190\u00b0C at a rate of 4\u00b0C min-1.\u00a0 The final
temperature of 300\u00b0C was reached by ramping to 275\u00b0C at 6\u00b0C
min-1 and then 15\u00b0C min-1 afterward.\u00a0 Samples were analyzed in
triplicate and normalized to the known \u03b415N values of a suite of 14 AAs
analyzed before and after each set of 3 samples.
attribute NC_GLOBAL awards_0_award_nid String 556513
attribute NC_GLOBAL awards_0_award_number String OCE-1260055
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1260055 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Zooplankton/phytoplankton CSIA-AA
Michael Landry, PI
Version 23 August 2018
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2018-08-22T18:54:54Z
attribute NC_GLOBAL date_modified String 2019-06-10T16:29:39Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.744468.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/744468 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Gas Chromatograph
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used with mass spectrometer
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 744477
attribute NC_GLOBAL instruments_0_description String Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB02/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Gas Chromatograph
attribute NC_GLOBAL instruments_0_instrument_nid String 661
attribute NC_GLOBAL instruments_0_supplied_name String Trace GC gas chromatograph, GC 1310 gas chromatograph
attribute NC_GLOBAL instruments_1_acronym String Gas Chromatograph
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Coupled to a GC Isolink combustion-reduction furnace (1000 deg C) and liquid nitrogen cold trap.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 744547
attribute NC_GLOBAL instruments_1_description String Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB02/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Gas Chromatograph
attribute NC_GLOBAL instruments_1_instrument_nid String 661
attribute NC_GLOBAL instruments_1_supplied_name String GC 1310
attribute NC_GLOBAL instruments_2_acronym String Mass Spec
attribute NC_GLOBAL instruments_2_dataset_instrument_description String Used for sampling
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 744476
attribute NC_GLOBAL instruments_2_description String General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.
attribute NC_GLOBAL instruments_2_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB16/ (external link)
attribute NC_GLOBAL instruments_2_instrument_name String Mass Spectrometer
attribute NC_GLOBAL instruments_2_instrument_nid String 685
attribute NC_GLOBAL instruments_2_supplied_name String Delta V Plus
attribute NC_GLOBAL keywords String ala, Ala_d15N, asp, Asp_d15N, bco, bco-dmo, biological, bulk, bulk_d15N, chemical, d15, data, dataset, dmo, erddap, experiment, glu, Glu_d15N, gly, Gly_d15N, iso, IsoL_d15N, leu, Leu_d15N, lys, Lys_d15N, management, note, Note_bulk_d15N, Note_Gly_d15N, Note_Organism, oceanography, office, organism, phe, Phe_d15N, preliminary, pro, Pro_d15N, ser, Ser_d15N, stage, thr, Thr_d15N, val, Val_d15N
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/744468/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/744468 (external link)
attribute NC_GLOBAL param_mapping String {'744468': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/744468/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of California-San Diego
attribute NC_GLOBAL people_0_affiliation_acronym String UCSD-SIO
attribute NC_GLOBAL people_0_person_name String Michael R. Landry
attribute NC_GLOBAL people_0_person_nid String 50545
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Hannah Ake
attribute NC_GLOBAL people_1_person_nid String 650173
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String CSIA-AA Mesozooplankton TP
attribute NC_GLOBAL projects_0_acronym String CSIA-AA Mesozooplankton TP
attribute NC_GLOBAL projects_0_description String Description from NSF award abstract:
Energy dissipation and elemental cycling by protistan consumers in lower trophic levels of ocean food webs are of sufficient magnitude, based on global mean measures of the amount of primary production consumed, to strongly alter the efficiencies of material transfers to higher-level consumers and to export. We presently know very little about these microbial food web steps, how they vary regionally or temporally, or how they might be altered by climate change. Compound Specific Isotope Analysis of Amino Acids (CSIA-AA) offers an approach for advancing our understanding of microbial food web structure and trophic fluxes based on the trophic positions (TP) of mesozooplankton as temporal integrators of the fluxes from direct feeding on phytoplankton and indirect transfers via protistan microzooplankton. Preliminary laboratory experiments to test this idea have demonstrated that the standard application of the method, using labeled phenylalanine as the representative source AA for the primary producer baseline and labeled glutamic acid as the indicator AA for trophic enrichment, does not produce a measureable trophic-step signal for protistan grazers. However, the results have also shown that an alternative high-turnover AA, alanine, strongly enriches in protistan as well as metazoan consumers, and leads to substantially higher TP estimates of mesozooplankton in field-collected specimens than that based on labeled glutamic acid.
This research project will test the hypothesis that labeled alanine provides a quantifiable and consistent index of trophic enrichment for protistan steps in marine food webs. The research will involve three major elements. First, controlled laboratory experiments will be conducted with chemostat systems to compare 15N enrichments of alanine to other AAs for a representative suite of ciliate and flagellate grazers feeding on phytoplankton, and to evaluate the two-step enrichment from phytoplankton via a protistan grazer to a suspension-feeding copepod. Second, field-collected mesozooplankton from four distinct ecological regions of the Pacific Ocean will be analyzed by CSIA-AA to test the transfer of alanine enrichment through a metazoan trophic step (comparing suspension feeding species to primary carnivores) and to assess how the TP index differs with trophic structure over a broad range of ecological conditions. Last, CSIA-AA assessments of TP for size-structured zooplankton will be integrated into inverse models of nitrogen flows in the four regions (equatorial Pacific, subtropical North Pacific, California Current and Costa Rica Dome) as a major constraint for resolving and comparing fluxes through the microbial food web over the range of ecological conditions. A properly calibrated CSIA-AA assessment of mesozooplankton trophic position will provide a new and valuable approach for regional intercomparisons of lower-level food web structure, for assessing temporal and spatial trends in climate change, for ocean ecosystem model validation, and for better understanding of lower food-web energetic constraints on ocean fisheries.
attribute NC_GLOBAL projects_0_end_date String 2018-03
attribute NC_GLOBAL projects_0_geolocation String California Current, eastern Pacific Ocean
attribute NC_GLOBAL projects_0_name String Resolving the trophic connection between protistan grazers and mesozooplankton in marine food webs using amino acid-specific stable isotope analyses
attribute NC_GLOBAL projects_0_project_nid String 556514
attribute NC_GLOBAL projects_0_start_date String 2013-04
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String CSIA 15N AA data from phytoplankton, microzooplankton, and Calanus pacificus.
attribute NC_GLOBAL title String CSIA 15N AA data from phytoplankton, microzooplankton, and Calanus pacificus.
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Experiment   byte  
attribute Experiment _FillValue byte 127
attribute Experiment actual_range byte 3, 6
attribute Experiment bcodmo_name String exp_id
attribute Experiment description String Experiment number
attribute Experiment long_name String Experiment
attribute Experiment units String unitless
variable Organism   String  
attribute Organism bcodmo_name String species
attribute Organism description String Species name or Particulate organic matter (POM)
attribute Organism long_name String Organism
attribute Organism units String unitless
variable stage   String  
attribute stage bcodmo_name String exp_type
attribute stage description String Refers to the experimental stage
attribute stage long_name String Stage
attribute stage units String unitless
variable bulk_d15N   String  
attribute bulk_d15N bcodmo_name String d15N
attribute bulk_d15N description String 15N of bulk material
attribute bulk_d15N long_name String Bulk D15 N
attribute bulk_d15N units String per mil
variable Ala_d15N   String  
attribute Ala_d15N bcodmo_name String d15N
attribute Ala_d15N description String 15N of AA Alanine
attribute Ala_d15N long_name String Ala D15 N
attribute Ala_d15N units String per mil
variable Asp_d15N   String  
attribute Asp_d15N bcodmo_name String d15N
attribute Asp_d15N description String 15N of AA Aspartic acid
attribute Asp_d15N long_name String Asp D15 N
attribute Asp_d15N units String per mil
variable Glu_d15N   String  
attribute Glu_d15N bcodmo_name String d15N
attribute Glu_d15N description String 15N of AA Glutamic acid
attribute Glu_d15N long_name String Glu D15 N
attribute Glu_d15N units String per mil
variable IsoL_d15N   String  
attribute IsoL_d15N bcodmo_name String d15N
attribute IsoL_d15N description String 15N of AA IsoLeucine
attribute IsoL_d15N long_name String Iso L D15 N
attribute IsoL_d15N units String per mil
variable Leu_d15N   String  
attribute Leu_d15N bcodmo_name String d15N
attribute Leu_d15N description String 15N of AA Leucine
attribute Leu_d15N long_name String Leu D15 N
attribute Leu_d15N units String per mil
variable Pro_d15N   String  
attribute Pro_d15N bcodmo_name String d15N
attribute Pro_d15N description String 15N of AA Proline
attribute Pro_d15N long_name String Pro D15 N
attribute Pro_d15N units String per mil
variable Val_d15N   String  
attribute Val_d15N bcodmo_name String d15N
attribute Val_d15N description String 15N of AA Valine
attribute Val_d15N long_name String Val D15 N
attribute Val_d15N units String per mil
variable Gly_d15N   String  
attribute Gly_d15N bcodmo_name String d15N
attribute Gly_d15N description String 15N of AA Glycine
attribute Gly_d15N long_name String GLY D15 N
attribute Gly_d15N units String per mil
variable Lys_d15N   String  
attribute Lys_d15N bcodmo_name String d15N
attribute Lys_d15N description String 15N of AA Lysine
attribute Lys_d15N long_name String LYS D15 N
attribute Lys_d15N units String per mil
variable Phe_d15N   String  
attribute Phe_d15N bcodmo_name String d15N
attribute Phe_d15N description String 15N of AA Phenylalanine
attribute Phe_d15N long_name String Phe D15 N
attribute Phe_d15N units String per mil
variable Ser_d15N   String  
attribute Ser_d15N bcodmo_name String d15N
attribute Ser_d15N description String 15N of AA Serine
attribute Ser_d15N long_name String Ser D15 N
attribute Ser_d15N units String per mil
variable Thr_d15N   String  
attribute Thr_d15N bcodmo_name String d15N
attribute Thr_d15N description String 15N of AA Treonine
attribute Thr_d15N long_name String THR D15 N
attribute Thr_d15N units String per mil
variable Note_Organism   String  
attribute Note_Organism bcodmo_name String comment
attribute Note_Organism description String Each note corresponds to the cell in this row in the Organism column
attribute Note_Organism long_name String Note Organism
attribute Note_Organism units String unitless
variable Note_bulk_d15N   String  
attribute Note_bulk_d15N bcodmo_name String comment
attribute Note_bulk_d15N description String Each note corresponds to the cell in this row in the bulk d15N column
attribute Note_bulk_d15N long_name String Note Bulk D15 N
attribute Note_bulk_d15N units String unitless
variable Note_Gly_d15N   String  
attribute Note_Gly_d15N bcodmo_name String comment
attribute Note_Gly_d15N description String Each note corresponds to the cell in this row in the Gly d15N column
attribute Note_Gly_d15N long_name String Note Gly D15 N
attribute Note_Gly_d15N units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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