ERDDAP > info > bcodmo_dataset_744468

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data			files	public	[Zooplankton/phytoplankton CSIA-AA] - CSIA 15N AA data from phytoplankton, microzooplankton, and Calanus pacificus. (Resolving the trophic connection between protistan grazers and mesozooplankton in marine food webs using amino acid-specific stable isotope analyses)		I   M	background			BCO-DMO	bcodmo_dataset_744468

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Hydrolysis, derivatization and analyses of samples for CSIA \u2013\u00a0 For Exps. 3-4, samples (1-2 mg dry weight) for CSIA-AA were added to 1 ml 6 N HPLC-grade HCl, then flushed with N2, capped with a Teflon-lined cap, and hydrolyzed at 150\u00b0C for 70 min.\u00a0 The resulting hydrolysate was evaporated to dryness under N2 at 55\u00b0C, redissolved in 1 ml 0.01 N HCl, purified by filtration (0.45-\u03bcm hydrophilic filter), washed with 1 ml of 0.01 N HCl, and further purified using cation-exchange chromatography with a 5-cm resin column (Dowex 50WX8-400) in a glass Pasteur pipette.\u00a0 AAs were eluted with 4 ml of 2 N NH4OH and evaporated to dryness under a stream of N2 at 80\u00b0C, then reacidified with 0.5 ml of 0.2 N HCl, flushed with N2, heated to 110\u00b0C for 5 min and evaporated to dryness under N2 at 55\u00b0C.\u00a0 Hydrolyzed samples were esterified with 2 ml of 4:1 isopropanol:acetyl chloride, flushed with N2 and heated to 110\u00b0C for 60 min.\u00a0 After drying at 60\u00b0C under N2, the samples were acylated by adding 1 ml of 3:1 methylene chloride:trifluoracetic anhydride (TFAA) and heated to 100\u00b0C for 15 min.\u00a0 The derivatized AAs were further purified by solvent extraction following (Ueda et al. 1989).\u00a0 The acylated AA esters were evaporated at room temperature under N2 and redissolved in 3 ml of 1:2 chloroform:P-buffer (KH2PO4 + Na2HPO4 in Milli-Q water, pH 7).\u00a0 Vigorous shaking ensured that the derivitized AAs were partitioned into chloroform while contaminants remained in the P-buffer.\u00a0 The solvents were separated by centrifugation (10 min at 600 g), the chloroform was transferred to a clean vial, and the solvent extraction process repeated.\u00a0 Finally, to ensure derivatization, the acylation step was repeated.\u00a0 Samples were stored at \u201320\u00b0C in 3:1 methylene chloride:TFAA for up to 2 weeks until analysis.\u00a0 AA derivatives were then analyzed by isotope monitoring gas chromatography-mass spectrometry.\u00a0 We used a Delta V Plus mass spectrometer interfaced with a Trace GC gas chromatograph through a GC-C III combustion furnace (980\u00b0C), reduction furnace (650\u00b0C), and liquid nitrogen cold trap.\u00a0 Internal reference compounds (aminoadipic acid and norleucine) of known nitrogen isotopic composition were co-injected with samples and used to normalize the measured \u03b415N values of unknown AAs, and a suite of eight AAs with known isotopic composition was analyzed every 3 injections for additional quality control.\u00a0 At least three injections per sample were analyzed.\u00a0 For Exps. 5 and 6, CSIA-AA samples were hydrolyzed in 6N HCl at 110\u00b0C for 20 h.\u00a0 The hydrolysate was evaporated to dryness at temperature under a continuous stream of N2 gas, then esterified with 4:1 isotopropanol:acetyl chloride at 110\u00b0C for 60 min, acylated in a 1:1 solution of methylene chloride:trifluoracetic anhydride (DCM:TFAA) for 10 min at 110\u00b0C.\u00a0 Samples were stored at -20\u00b0C in 1:1 DCM:TFAA for up to 3 months before isotope analysis using a Delta V Plus mass spectrometer (Thermo Scientific) interfaced through a Conflo IV to a GC 1310 gas chromatograph coupled to a GC Isolink combustion-reduction furnace (1000\u00b0C) and liquid nitrogen cold trap.\u00a0 All samples were injected (splitless injector) onto a forte BPx5 capillary column (60 m x 0.32 mm x 1.0-\u03bcm film thickness) at an injector temperature of 250\u00b0C with a constant helium flow rate of 1.4 ml min\u20131.\u00a0 The column was initially held at 50\u00b0C for 2 min and then increased to 125 \u00b0C at a rate of 15\u00b0C min-1.\u00a0 Once at 125\u00b0C, the temperature was increased at a rate of 3\u00b0C min-1 to 160\u00b0C and then to 190\u00b0C at a rate of 4\u00b0C min-1.\u00a0 The final temperature of 300\u00b0C was reached by ramping to 275\u00b0C at 6\u00b0C min-1 and then 15\u00b0C min-1 afterward.\u00a0 Samples were analyzed in triplicate and normalized to the known \u03b415N values of a suite of 14 AAs analyzed before and after each set of 3 samples.
attribute	NC_GLOBAL	awards_0_award_nid	String	556513
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1260055
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1260055
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Zooplankton/phytoplankton CSIA-AA Michael Landry, PI Version 23 August 2018
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2018-08-22T18:54:54Z
attribute	NC_GLOBAL	date_modified	String	2019-06-10T16:29:39Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.744468.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/744468
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Gas Chromatograph
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used with mass spectrometer
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	744477
attribute	NC_GLOBAL	instruments_0_description	String	Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB02/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Gas Chromatograph
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	661
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Trace GC gas chromatograph, GC 1310 gas chromatograph
attribute	NC_GLOBAL	instruments_1_acronym	String	Gas Chromatograph
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Coupled to a GC Isolink combustion-reduction furnace (1000 deg C) and liquid nitrogen cold trap.
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	744547
attribute	NC_GLOBAL	instruments_1_description	String	Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB02/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Gas Chromatograph
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	661
attribute	NC_GLOBAL	instruments_1_supplied_name	String	GC 1310
attribute	NC_GLOBAL	instruments_2_acronym	String	Mass Spec
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Used for sampling
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	744476
attribute	NC_GLOBAL	instruments_2_description	String	General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.
attribute	NC_GLOBAL	instruments_2_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB16/
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Mass Spectrometer
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	685
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Delta V Plus
attribute	NC_GLOBAL	keywords	String	ala, Ala_d15N, asp, Asp_d15N, bco, bco-dmo, biological, bulk, bulk_d15N, chemical, d15, data, dataset, dmo, erddap, experiment, glu, Glu_d15N, gly, Gly_d15N, iso, IsoL_d15N, leu, Leu_d15N, lys, Lys_d15N, management, note, Note_bulk_d15N, Note_Gly_d15N, Note_Organism, oceanography, office, organism, phe, Phe_d15N, preliminary, pro, Pro_d15N, ser, Ser_d15N, stage, thr, Thr_d15N, val, Val_d15N
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/744468/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/744468
attribute	NC_GLOBAL	param_mapping	String	{'744468': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/744468/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of California-San Diego
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UCSD-SIO
attribute	NC_GLOBAL	people_0_person_name	String	Michael R. Landry
attribute	NC_GLOBAL	people_0_person_nid	String	50545
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Hannah Ake
attribute	NC_GLOBAL	people_1_person_nid	String	650173
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	CSIA-AA Mesozooplankton TP
attribute	NC_GLOBAL	projects_0_acronym	String	CSIA-AA Mesozooplankton TP
attribute	NC_GLOBAL	projects_0_description	String	Description from NSF award abstract: Energy dissipation and elemental cycling by protistan consumers in lower trophic levels of ocean food webs are of sufficient magnitude, based on global mean measures of the amount of primary production consumed, to strongly alter the efficiencies of material transfers to higher-level consumers and to export. We presently know very little about these microbial food web steps, how they vary regionally or temporally, or how they might be altered by climate change. Compound Specific Isotope Analysis of Amino Acids (CSIA-AA) offers an approach for advancing our understanding of microbial food web structure and trophic fluxes based on the trophic positions (TP) of mesozooplankton as temporal integrators of the fluxes from direct feeding on phytoplankton and indirect transfers via protistan microzooplankton. Preliminary laboratory experiments to test this idea have demonstrated that the standard application of the method, using labeled phenylalanine as the representative source AA for the primary producer baseline and labeled glutamic acid as the indicator AA for trophic enrichment, does not produce a measureable trophic-step signal for protistan grazers. However, the results have also shown that an alternative high-turnover AA, alanine, strongly enriches in protistan as well as metazoan consumers, and leads to substantially higher TP estimates of mesozooplankton in field-collected specimens than that based on labeled glutamic acid. This research project will test the hypothesis that labeled alanine provides a quantifiable and consistent index of trophic enrichment for protistan steps in marine food webs. The research will involve three major elements. First, controlled laboratory experiments will be conducted with chemostat systems to compare 15N enrichments of alanine to other AAs for a representative suite of ciliate and flagellate grazers feeding on phytoplankton, and to evaluate the two-step enrichment from phytoplankton via a protistan grazer to a suspension-feeding copepod. Second, field-collected mesozooplankton from four distinct ecological regions of the Pacific Ocean will be analyzed by CSIA-AA to test the transfer of alanine enrichment through a metazoan trophic step (comparing suspension feeding species to primary carnivores) and to assess how the TP index differs with trophic structure over a broad range of ecological conditions. Last, CSIA-AA assessments of TP for size-structured zooplankton will be integrated into inverse models of nitrogen flows in the four regions (equatorial Pacific, subtropical North Pacific, California Current and Costa Rica Dome) as a major constraint for resolving and comparing fluxes through the microbial food web over the range of ecological conditions. A properly calibrated CSIA-AA assessment of mesozooplankton trophic position will provide a new and valuable approach for regional intercomparisons of lower-level food web structure, for assessing temporal and spatial trends in climate change, for ocean ecosystem model validation, and for better understanding of lower food-web energetic constraints on ocean fisheries.
attribute	NC_GLOBAL	projects_0_end_date	String	2018-03
attribute	NC_GLOBAL	projects_0_geolocation	String	California Current, eastern Pacific Ocean
attribute	NC_GLOBAL	projects_0_name	String	Resolving the trophic connection between protistan grazers and mesozooplankton in marine food webs using amino acid-specific stable isotope analyses
attribute	NC_GLOBAL	projects_0_project_nid	String	556514
attribute	NC_GLOBAL	projects_0_start_date	String	2013-04
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	CSIA 15N AA data from phytoplankton, microzooplankton, and Calanus pacificus.
attribute	NC_GLOBAL	title	String	[Zooplankton/phytoplankton CSIA-AA] - CSIA 15N AA data from phytoplankton, microzooplankton, and Calanus pacificus. (Resolving the trophic connection between protistan grazers and mesozooplankton in marine food webs using amino acid-specific stable isotope analyses)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Experiment		byte
attribute	Experiment	_FillValue	byte	127
attribute	Experiment	actual_range	byte	3, 6
attribute	Experiment	bcodmo_name	String	exp_id
attribute	Experiment	description	String	Experiment number
attribute	Experiment	long_name	String	Experiment
attribute	Experiment	units	String	unitless
variable	Organism		String
attribute	Organism	bcodmo_name	String	species
attribute	Organism	description	String	Species name or Particulate organic matter (POM)
attribute	Organism	long_name	String	Organism
attribute	Organism	units	String	unitless
variable	stage		String
attribute	stage	bcodmo_name	String	exp_type
attribute	stage	description	String	Refers to the experimental stage
attribute	stage	long_name	String	Stage
attribute	stage	units	String	unitless
variable	bulk_d15N		String
attribute	bulk_d15N	bcodmo_name	String	d15N
attribute	bulk_d15N	description	String	15N of bulk material
attribute	bulk_d15N	long_name	String	Bulk D15 N
attribute	bulk_d15N	units	String	per mil
variable	Ala_d15N		String
attribute	Ala_d15N	bcodmo_name	String	d15N
attribute	Ala_d15N	description	String	15N of AA Alanine
attribute	Ala_d15N	long_name	String	Ala D15 N
attribute	Ala_d15N	units	String	per mil
variable	Asp_d15N		String
attribute	Asp_d15N	bcodmo_name	String	d15N
attribute	Asp_d15N	description	String	15N of AA Aspartic acid
attribute	Asp_d15N	long_name	String	Asp D15 N
attribute	Asp_d15N	units	String	per mil
variable	Glu_d15N		String
attribute	Glu_d15N	bcodmo_name	String	d15N
attribute	Glu_d15N	description	String	15N of AA Glutamic acid
attribute	Glu_d15N	long_name	String	Glu D15 N
attribute	Glu_d15N	units	String	per mil
variable	IsoL_d15N		String
attribute	IsoL_d15N	bcodmo_name	String	d15N
attribute	IsoL_d15N	description	String	15N of AA IsoLeucine
attribute	IsoL_d15N	long_name	String	Iso L D15 N
attribute	IsoL_d15N	units	String	per mil
variable	Leu_d15N		String
attribute	Leu_d15N	bcodmo_name	String	d15N
attribute	Leu_d15N	description	String	15N of AA Leucine
attribute	Leu_d15N	long_name	String	Leu D15 N
attribute	Leu_d15N	units	String	per mil
variable	Pro_d15N		String
attribute	Pro_d15N	bcodmo_name	String	d15N
attribute	Pro_d15N	description	String	15N of AA Proline
attribute	Pro_d15N	long_name	String	Pro D15 N
attribute	Pro_d15N	units	String	per mil
variable	Val_d15N		String
attribute	Val_d15N	bcodmo_name	String	d15N
attribute	Val_d15N	description	String	15N of AA Valine
attribute	Val_d15N	long_name	String	Val D15 N
attribute	Val_d15N	units	String	per mil
variable	Gly_d15N		String
attribute	Gly_d15N	bcodmo_name	String	d15N
attribute	Gly_d15N	description	String	15N of AA Glycine
attribute	Gly_d15N	long_name	String	GLY D15 N
attribute	Gly_d15N	units	String	per mil
variable	Lys_d15N		String
attribute	Lys_d15N	bcodmo_name	String	d15N
attribute	Lys_d15N	description	String	15N of AA Lysine
attribute	Lys_d15N	long_name	String	LYS D15 N
attribute	Lys_d15N	units	String	per mil
variable	Phe_d15N		String
attribute	Phe_d15N	bcodmo_name	String	d15N
attribute	Phe_d15N	description	String	15N of AA Phenylalanine
attribute	Phe_d15N	long_name	String	Phe D15 N
attribute	Phe_d15N	units	String	per mil
variable	Ser_d15N		String
attribute	Ser_d15N	bcodmo_name	String	d15N
attribute	Ser_d15N	description	String	15N of AA Serine
attribute	Ser_d15N	long_name	String	Ser D15 N
attribute	Ser_d15N	units	String	per mil
variable	Thr_d15N		String
attribute	Thr_d15N	bcodmo_name	String	d15N
attribute	Thr_d15N	description	String	15N of AA Treonine
attribute	Thr_d15N	long_name	String	THR D15 N
attribute	Thr_d15N	units	String	per mil
variable	Note_Organism		String
attribute	Note_Organism	bcodmo_name	String	comment
attribute	Note_Organism	description	String	Each note corresponds to the cell in this row in the Organism column
attribute	Note_Organism	long_name	String	Note Organism
attribute	Note_Organism	units	String	unitless
variable	Note_bulk_d15N		String
attribute	Note_bulk_d15N	bcodmo_name	String	comment
attribute	Note_bulk_d15N	description	String	Each note corresponds to the cell in this row in the bulk d15N column
attribute	Note_bulk_d15N	long_name	String	Note Bulk D15 N
attribute	Note_bulk_d15N	units	String	unitless
variable	Note_Gly_d15N		String
attribute	Note_Gly_d15N	bcodmo_name	String	comment
attribute	Note_Gly_d15N	description	String	Each note corresponds to the cell in this row in the Gly d15N column
attribute	Note_Gly_d15N	long_name	String	Note Gly D15 N
attribute	Note_Gly_d15N	units	String	unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.