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     data   graph     files  public [Coastal SAR11 Physiochemical data] - Physiochemical data from samples collected along the
coast of Louisiana, USA during 2018 (Collaborative Research: EAGER: Salinity-based selection
between sister clades of abundant coastal bacterioplankton)
   ?   F   I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_745449

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson,.odvTxt
attribute NC_GLOBAL acquisition_description String Samples were collected manually by filling an acid-washed and autoclaved 20L
carboy after three rinses. Temperature, pH, and salinity were taken using a
handheld YSI. Cell counts were obtained by filtering water through a 2.7
\u00b5m Whatman GF/D filter, fixing with 10% formaldehyde, placing on ice, and
then counting using flow cytometry (Thrash et al., 2015, Hydrocarbon and Lipid
Microbiology Protocols). Inorganic nutrients were measured at the University
of Washington Marine Chemistry Laboratory after sequential filtration through
2.7 Whatman GF/D and 0.22 \u00b5m Sterivex filters. Samples were initially
placed on ice in the field, and then refrigerated until shipment with ice
packs.
attribute NC_GLOBAL awards_0_award_nid String 707004
attribute NC_GLOBAL awards_0_award_number String OCE-1747681
attribute NC_GLOBAL awards_0_data_url String https://www.nsf.gov/awardsearch/showAward?AWD_ID=1747681 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL awards_1_award_nid String 707005
attribute NC_GLOBAL awards_1_award_number String OCE-1747722
attribute NC_GLOBAL awards_1_data_url String https://www.nsf.gov/awardsearch/showAward?AWD_ID=1747722 (external link)
attribute NC_GLOBAL awards_1_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_1_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_1_funding_source_nid String 355
attribute NC_GLOBAL awards_1_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_1_program_manager_nid String 50446
attribute NC_GLOBAL awards_2_award_nid String 787323
attribute NC_GLOBAL awards_2_award_number String OCE-1931113
attribute NC_GLOBAL awards_2_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1931113 (external link)
attribute NC_GLOBAL awards_2_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_2_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_2_funding_source_nid String 355
attribute NC_GLOBAL awards_2_program_manager String Daniel Thornhill
attribute NC_GLOBAL awards_2_program_manager_nid String 722161
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Physiochemical data for coastal samples
PI: J. Cameron Thrash (USC)
Co-PI: Elizabeth B. Kujawinksi (WHOI)
Version history:
2020-02-13 - version 2 (current)
2018-08-30 - version 1
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2018-08-30T19:52:45Z
attribute NC_GLOBAL date_modified String 2020-02-14T15:37:08Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.745449.2
attribute NC_GLOBAL Easternmost_Easting double -89.676029
attribute NC_GLOBAL geospatial_lat_max double 29.867989
attribute NC_GLOBAL geospatial_lat_min double 29.243342
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -89.676029
attribute NC_GLOBAL geospatial_lon_min double -93.340714
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/745449 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Flow Cytometer
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used for sampling
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 745484
attribute NC_GLOBAL instruments_0_description String Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB37/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Flow Cytometer
attribute NC_GLOBAL instruments_0_instrument_nid String 660
attribute NC_GLOBAL instruments_0_supplied_name String Millipore Guava 5HT HPL benchtop flow cytometer
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Used for sampling
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 745485
attribute NC_GLOBAL instruments_1_description String A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
attribute NC_GLOBAL instruments_1_instrument_name String Pump
attribute NC_GLOBAL instruments_1_instrument_nid String 726
attribute NC_GLOBAL instruments_1_supplied_name String Masterflex I/P peristaltic pump
attribute NC_GLOBAL instruments_2_dataset_instrument_description String YSI 556 MPS handheld meter, calibrated with salinity and pH standards immediately prior to use
attribute NC_GLOBAL instruments_2_dataset_instrument_nid String 745486
attribute NC_GLOBAL instruments_2_description String An analytical instrument that can measure multiple parameters, such as pH, EC, TDS, DO and temperature with one device and is portable or hand-held.
attribute NC_GLOBAL instruments_2_instrument_name String Multi Parameter Portable Meter
attribute NC_GLOBAL instruments_2_instrument_nid String 489457
attribute NC_GLOBAL instruments_2_supplied_name String YSI 556 MPS handheld meter
attribute NC_GLOBAL keywords String ammonia, ammonium, bco, bco-dmo, biological, cell, Cell_counts, central, chemical, chemistry, concentration, cond, counts, data, dataset, date, density, dmo, earth, Earth Science > Oceans > Ocean Chemistry > Ammonia, Earth Science > Oceans > Ocean Chemistry > Nitrate, Earth Science > Oceans > Ocean Chemistry > pH, Earth Science > Oceans > Ocean Chemistry > Phosphate, Earth Science > Oceans > Salinity/Density > Salinity, erddap, iso, latitude, longitude, management, mass, mass_concentration_of_phosphate_in_sea_water, mole, mole_concentration_of_ammonium_in_sea_water, mole_concentration_of_nitrate_in_sea_water, mole_concentration_of_nitrite_in_sea_water, n02, nh4, nitrate, nitrite, NO2, no3, ocean, oceanography, oceans, office, offset, oh4, phosphate, po4, practical, preliminary, reported, salinity, scale, science, sea, sea_water_ph_reported_on_total_scale, sea_water_practical_salinity, seawater, SiOH4, site, Temp, temperature, time, Time_Central, Time_UTC, total, water
attribute NC_GLOBAL keywords_vocabulary String GCMD Science Keywords
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/745449/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/745449 (external link)
attribute NC_GLOBAL Northernmost_Northing double 29.867989
attribute NC_GLOBAL param_mapping String {'745449': {'Lat': 'flag - latitude', 'Lon': 'flag - longitude', 'ISO_DateTime_UTC': 'flag - time'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/745449/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Southern California
attribute NC_GLOBAL people_0_affiliation_acronym String USC
attribute NC_GLOBAL people_0_person_name String J. Cameron Thrash
attribute NC_GLOBAL people_0_person_nid String 707009
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI
attribute NC_GLOBAL people_1_person_name String Elizabeth Kujawinski
attribute NC_GLOBAL people_1_person_nid String 51089
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of Southern California
attribute NC_GLOBAL people_2_affiliation_acronym String USC
attribute NC_GLOBAL people_2_person_name String J. Cameron Thrash
attribute NC_GLOBAL people_2_person_nid String 707009
attribute NC_GLOBAL people_2_role String Contact
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Shannon Rauch
attribute NC_GLOBAL people_3_person_nid String 51498
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String CoastalSAR11
attribute NC_GLOBAL projects_0_acronym String CoastalSAR11
attribute NC_GLOBAL projects_0_description String NSF award abstract:
Adaptation to new environments is a fundamental challenge for organisms, including microbes, in expanding their habitat range. It is important to investigate the cellular mechanisms underlying salinity tolerance in coastal bacterioplankton and their different responses to salinity in nature because (i) it will provide fundamental understanding for how microorganisms evolve to inhabit environments with different salinities, and (ii) alterations in coastal salinity are connected to climate change, so the way these alterations affect abundant coastal microorganisms also alters the biogeochemical cycling of, e.g., carbon. The project will examine microbial adaptations to salinity and determine how changes in salinity affect microbial metabolism using two closely related groups of abundant coastal bacterioplankton as model taxa. In addition, the research will continue and expand microbiology Course-based Undergraduate Research Experiences (mCUREs) in high-throughput cultivation and microbial characterization at the Lousiana State University. Sections of freshman biology laboratories will learn how to isolate, characterize, and molecularly identify microorganisms from local aquatic systems. mCURE sections will lead to newly isolated strains, genome sequences, and physiological data, these results will be published with the contributing students as co-authors. The relative success of mCURE sections will be assessed compared to traditional freshman biology sections. mCURE sections will offer unique opportunities for LSU students by creating excitement about research through discovery of new organisms and generating knowledge of the coastal habitats that are essential to the livelihood of the Gulf Coast.
The evolutionary transition between salt- and freshwater environments occurs rarely in microorganisms. In one of the most abundant aquatic groups, SAR11, the transition between salt- and freshwater environments has happened only once: all freshwater SAR11 belong to subclade IIIb/LD12, which has also been found to inhabit coastal environments where salinity varies widely. The first reported isolates of the SAR11 freshwater clade LD12 and a member of the sister clade IIIa from the same region are now available. These pure culture representatives provide a powerful model for experimentally investigating adaptations to new environments in microorganisms, specifically (i) the genomic pathway and regulatory distinctions that arise during the evolutionary transition from marine to freshwater environments, and (ii) the physiological mechanisms that underlie the ecological restrictions imposed on microorganisms by ionic strength in coastal and freshwater environments. Furthermore, because these organisms have distinct differences in metabolic potential, the isolates facilitate testing (iii) the effects of changing coastal salinity on microbial contributions to other biogeochemical cycles, such as that for carbon. The project will test the hypothesis that the relative ionic strength tolerances between the sister lineages (LD12, IIIa) result from fundamental differences in metabolic flexibility at a genomic and regulatory level. To do so it will assess transcriptional and metabolic responses to varied ionic strength for both taxa and measure the distribution and activity of both groups in nature to translate laboratory findings to the field. The research will provide new understanding of LD12 habitat range and insights into how the "freshwater" lineage evolved from a SAR11 common ancestor. The project will also more generally provide important information on microbial responses to salinity changes in coastal systems and the evolutionary paths separating freshwater and marine microorganisms.
This award is co-funded by Biological Oceanography, Division of Ocean Sciences in the Directorate for Geosciences and by Systems and Synthetic Biology, Division of Molecular and Cellular Biosciences in the Directorate for Biological Sciences.
attribute NC_GLOBAL projects_0_end_date String 2020-12
attribute NC_GLOBAL projects_0_geolocation String Coastal Louisiana, northern Gulf of Mexico
attribute NC_GLOBAL projects_0_name String Collaborative Research: EAGER: Salinity-based selection between sister clades of abundant coastal bacterioplankton
attribute NC_GLOBAL projects_0_project_nid String 707006
attribute NC_GLOBAL projects_0_start_date String 2018-01
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 29.243342
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Physiochemical data from samples collected along the coast of Louisiana, USA during 2018.
attribute NC_GLOBAL time_coverage_end String 2018-11-16T20:00:00Z
attribute NC_GLOBAL time_coverage_start String 2018-01-27T16:53:00Z
attribute NC_GLOBAL title String [Coastal SAR11 Physiochemical data] - Physiochemical data from samples collected along the coast of Louisiana, USA during 2018 (Collaborative Research: EAGER: Salinity-based selection between sister clades of abundant coastal bacterioplankton)
attribute NC_GLOBAL version String 2
attribute NC_GLOBAL Westernmost_Easting double -93.340714
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Site   String  
attribute Site bcodmo_name String site
attribute Site description String Sampling site designation
attribute Site long_name String Site
attribute Site units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 29.243342, 29.867989
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude of site
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -93.340714, -89.676029
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude of site
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable Date   String  
attribute Date bcodmo_name String date
attribute Date description String Date of sampling; format: yyyy-mm-dd
attribute Date long_name String Date
attribute Date nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute Date time_precision String 1970-01-01
attribute Date units String unitless
variable Time_Central   String  
attribute Time_Central bcodmo_name String time
attribute Time_Central description String Time of sampling in the Central Time Zone, USA; format: HH:MM
attribute Time_Central long_name String Time Central
attribute Time_Central nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/AHMSAA01/ (external link)
attribute Time_Central units String unitless
variable Offset   String  
attribute Offset bcodmo_name String time_offset
attribute Offset description String Universal time coordinated (UTC) offset, corrected for daylight savings
attribute Offset long_name String Offset
attribute Offset units String unitless
variable Time_UTC   String  
attribute Time_UTC bcodmo_name String time
attribute Time_UTC description String Universal Time Coordinated (UTC) subtracting Central Time from the UTC offset; format: HH:MM
attribute Time_UTC long_name String Time UTC
attribute Time_UTC nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/AHMSAA01/ (external link)
attribute Time_UTC units String unitless
variable Temp   float  
attribute Temp _FillValue float NaN
attribute Temp actual_range float 9.38, 32.81
attribute Temp bcodmo_name String temperature
attribute Temp description String Temperature measured via YSI at the sampling site
attribute Temp long_name String Temperature
attribute Temp units String degrees Celsius
variable Cond   float  
attribute Cond _FillValue float NaN
attribute Cond actual_range float 0.279, 48.77
attribute Cond bcodmo_name String conductivity
attribute Cond description String Conductivity measured via YSI at the sampling site
attribute Cond long_name String Cond
attribute Cond nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/CNDC/ (external link)
attribute Cond units String ms/cm
variable Salinity   float  
attribute Salinity _FillValue float NaN
attribute Salinity actual_range float 0.12, 31.72
attribute Salinity bcodmo_name String sal
attribute Salinity colorBarMaximum double 37.0
attribute Salinity colorBarMinimum double 32.0
attribute Salinity description String Calculated salinity based on the conductivity measurement from the YSI
attribute Salinity long_name String Sea Water Practical Salinity
attribute Salinity nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PSALST01/ (external link)
attribute Salinity units String unitless
variable pH   float  
attribute pH _FillValue float NaN
attribute pH actual_range float 7.32, 8.84
attribute pH bcodmo_name String pH
attribute pH colorBarMaximum double 9.0
attribute pH colorBarMinimum double 7.0
attribute pH description String pH of the seawater at the sampling site
attribute pH long_name String Sea Water Ph Reported On Total Scale
attribute pH nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute pH units String unitless
variable Cell_counts   float  
attribute Cell_counts _FillValue float NaN
attribute Cell_counts actual_range float 1930000.0, 1.52E7
attribute Cell_counts bcodmo_name String count
attribute Cell_counts description String Number of cells passing through a 2.7 µm filter (Whatman GF/D) determined via flow cytometry
attribute Cell_counts long_name String Cell Counts
attribute Cell_counts units String cells/mL
variable PO4   float  
attribute PO4 _FillValue float NaN
attribute PO4 actual_range float 0.21, 2.55
attribute PO4 bcodmo_name String PO4
attribute PO4 description String Concentration of phosphate in seawater from the site
attribute PO4 long_name String Mass Concentration Of Phosphate In Sea Water
attribute PO4 units String umol/L
variable SiOH4   float  
attribute SiOH4 _FillValue float NaN
attribute SiOH4 actual_range float 1.14, 171.83
attribute SiOH4 bcodmo_name String SiOH_4
attribute SiOH4 description String Concentration of silicate in seawater from the site
attribute SiOH4 long_name String Si OH4
attribute SiOH4 units String umol/L
variable NO3   float  
attribute NO3 _FillValue float NaN
attribute NO3 actual_range float 0.31, 62.34
attribute NO3 bcodmo_name String NO3
attribute NO3 colorBarMaximum double 50.0
attribute NO3 colorBarMinimum double 0.0
attribute NO3 description String Concentration of nitrate in seawater from the site
attribute NO3 long_name String Mole Concentration Of Nitrate In Sea Water
attribute NO3 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/NTRAIGGS/ (external link)
attribute NO3 units String umol/L
variable NO2   float  
attribute NO2 _FillValue float NaN
attribute NO2 actual_range float 0.02, 5.18
attribute NO2 bcodmo_name String NO2
attribute NO2 colorBarMaximum double 1.0
attribute NO2 colorBarMinimum double 0.0
attribute NO2 description String Concentration of nitrite in seawater from the site
attribute NO2 long_name String Mole Concentration Of Nitrite In Sea Water
attribute NO2 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/NTRIAAZX/ (external link)
attribute NO2 units String umol/L
variable NH4   float  
attribute NH4 _FillValue float NaN
attribute NH4 actual_range float 0.02, 19.23
attribute NH4 bcodmo_name String Ammonium
attribute NH4 colorBarMaximum double 5.0
attribute NH4 colorBarMinimum double 0.0
attribute NH4 description String Concentration of ammonium in seawater from the site
attribute NH4 long_name String Mole Concentration Of Ammonium In Sea Water
attribute NH4 nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/AMONAAZX/ (external link)
attribute NH4 units String umol/L
variable time   double  
attribute time _CoordinateAxisType String Time
attribute time actual_range double 1.51707198E9, 1.5423984E9
attribute time axis String T
attribute time bcodmo_name String ISO_DateTime_UTC
attribute time description String Date and time of sampling (UTC) formatted to ISO 8601 standard; format: yyyy-mm-ddTHH:MMZ
attribute time ioos_category String Time
attribute time long_name String ISO Date Time UTC
attribute time nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/ (external link)
attribute time source_name String ISO_DateTime_UTC
attribute time standard_name String time
attribute time time_origin String 01-JAN-1970 00:00:00
attribute time time_precision String 1970-01-01T00:00:00Z
attribute time units String seconds since 1970-01-01T00:00:00Z

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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