ERDDAP > info > bcodmo_dataset_748445

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Hydrolytic enzyme activity - Coscinodiscus] - Hydrolytic enzyme activities during CDOM monoculture experiment with Coscinodiscus (Collaborative Research: Planktonic Sources of Chromophoric Dissolved Organic Matter in Seawater)		I   M	background			BCO-DMO	bcodmo_dataset_748445

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Hydrolytic enzyme activities were determined using L-leucine-4-methylcoumarinyl-7-amide (MCA) hydrochloride, 4-methylumbelliferyl \u03b1-D-glucopyranoside, and 4-methylumbelliferone (MUF) \u03b2-D-glucopyranoside (Sigma-Aldrich) as substrate proxies for leucine- aminopeptidase, \u03b1-glucosidase, and \u03b2-glucosidase activities, respectively. For each bottle and substrate proxy, 196 \u00b5L of unfiltered experimental or control water was added in duplicate to a pure-grade black 96-well plate (Brand Life Sciences) containing a single substrate proxy at saturation levels (final concentration 200 \u00b5M). Fluorescence (excitation 370 nm, emission 440 nm) was measured in a Tecan Infinite 200 Pro microplate reader immediately following the addition of the substrate and several more times over 7-20 h. The well plates were incubated in the dark at in situ temperature. MUF and MCA standard solutions prepared in seawater were used to determine hydrolysis rates. Killed controls (boiled sample water) and ultrapure water samples showed little change over the incubations.
attribute	NC_GLOBAL	awards_0_award_nid	String	734588
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1459406
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1459406
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Henrietta N Edmonds
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	51517
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Coscinodiscus hydrolytic enzyme activities during CDOM monoculture experiment PI: K. Ziervogel (UNH) version: 2018-10-17
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2018-10-18T13:20:58Z
attribute	NC_GLOBAL	date_modified	String	2019-03-18T15:43:27Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.748445.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/748445
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Flow Cytometer
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used to make cell counts.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	748450
attribute	NC_GLOBAL	instruments_0_description	String	Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB37/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Flow Cytometer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	660
attribute	NC_GLOBAL	instruments_0_supplied_name	String	FACSCalibur flow cytometer (Becton-Dickson)
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Used to measure fluorescence from which hydrolysis rates were calculated.
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	748451
attribute	NC_GLOBAL	instruments_1_description	String	Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: https://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	plate reader
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	528693
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Tecan Infinite 200 Pro microplate reader
attribute	NC_GLOBAL	keywords	String	activity, bco, bco-dmo, biological, chemical, data, dataset, dmo, enz, enz_activity_t0, enz_activity_t1, enz_activity_t2, enz_activity_t3, erddap, fluor, fluor_t0, fluor_t1, fluor_t2, fluor_t3, management, oceanography, office, preliminary, rsqr, sample, slope, substrate, time, time_t0, time_t1, time_t2, time_t3
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/748445/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/748445
attribute	NC_GLOBAL	param_mapping	String	{'748445': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/748445/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of New Hampshire
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UNH
attribute	NC_GLOBAL	people_0_person_name	String	Kai Ziervogel
attribute	NC_GLOBAL	people_0_person_nid	String	734583
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_1_person_nid	String	50396
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	PlankDOM
attribute	NC_GLOBAL	projects_0_acronym	String	PlankDOM
attribute	NC_GLOBAL	projects_0_description	String	NSF abstract: Chromophoric dissolved organic matter (CDOM) is a small but important fraction of the marine carbon pool that interacts with solar radiation and thus affects many photochemical and biological processes in the ocean. Despite its importance, the chemical basis for the formation of oceanic CDOM remains unclear. CDOM may be formed from two possible sources: 1) heterotrophic bacterial transformations of primary productivity (plankton-derived), or 2) terrestrially-derived. This project will examine the role of phytoplankton as a source of CDOM in the ocean by utilizing a powerful, new technique to measure particulate organic matter absorbance and fluorescence, discrete chemical measurements of probable precursors to planktonic CDOM, and enzymatic assays. Results of this research will provide new insights into the origin and production of planktonic CDOM and its transformation by heterotrophic bacteria. This research on CDOM will be shared broadly through a module at a North Carolina Aquarium, and streaming live feeds of shipboard activities to elementary school classrooms. Terrestrial and oceanic dissolved organic matter (DOM) differ in their chemical composition. Laboratory and open-ocean observations suggest that bacterial transformation of phytoplankton DOM produces humic-like CDOM signals that are visually similar to those in terrestrial CDOM. However, prior studies of oceanic CDOM using absorbance and fluorescence fit an electronic interaction (EI) model of intramolecular charge transfer (CT) reactions between donor and acceptor molecules common to partially-oxidized terrestrial molecules found in humic substances. This project will test the hypothesis that phytoplankton and bacteria provide a source of donors and acceptors that are microbially-transformed and linked, enabling CT contacts between them and creating oceanic CDOM. To address this, researchers will systematically study phytoplankton growth, including marine snow formation. A new technique for measuring base-extracted POM (BEPOM) absorbance and fluorescence will be used to incorporate planktonic CDOM results into the EI model, and supplemented with measurements of its probable chemical precursors. These experiments will improve understanding of how the production of CDOM in the ocean is linked to the optics and chemistry of planktonic CDOM formation. Determining the time course and extent of phytoplankton POM and DOM transformation by heterotrophic bacteria during the same phytoplankton growth experiments will provide an in-depth understanding as to how bacterial transformation of marine snow-associated planktonic organic matter drives CDOM production throughout the ocean.
attribute	NC_GLOBAL	projects_0_end_date	String	2019-04
attribute	NC_GLOBAL	projects_0_geolocation	String	Northern Atlantic Ocean, 34.65 N, 69.63 W
attribute	NC_GLOBAL	projects_0_name	String	Collaborative Research: Planktonic Sources of Chromophoric Dissolved Organic Matter in Seawater
attribute	NC_GLOBAL	projects_0_project_nid	String	734581
attribute	NC_GLOBAL	projects_0_start_date	String	2015-05
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	This dataset is from a laboratory experiment. Four phytoplankton cultures and their associated bacterial communities were incubated in replicate roller bottles (1.9 L) over 3-6 weeks under laboratory conditions. Bacterial dynamics in the culture bottles were measured and correlated with geochemical parameters to determine the role of bacterial activities on the formation of CDOM in the cultures (Kinsey et al., 2018, see below).\r\n\r\nThe data include fluorescence and bacterial enzyme activity during CDOM Coscinodiscus monoculture experiments. Growth stages were initial and exponential.
attribute	NC_GLOBAL	title	String	[Hydrolytic enzyme activity - Coscinodiscus] - Hydrolytic enzyme activities during CDOM monoculture experiment with Coscinodiscus (Collaborative Research: Planktonic Sources of Chromophoric Dissolved Organic Matter in Seawater)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	substrate		String
attribute	substrate	bcodmo_name	String	unknown
attribute	substrate	description	String	substrate for measuring enzyme activity: a-glu = 4-methylumbelliferyl a-D-glucopyranoside; b-glu = 4-methylumbelliferone (MUF) ß-D-glucopyranoside; leu = L-leucine-4-methylcoumarinyl-7-amide
attribute	substrate	long_name	String	Substrate
attribute	substrate	units	String	unitless
variable	sample		String
attribute	sample	bcodmo_name	String	sample
attribute	sample	description	String	sample identifier denoted as growth stage (days from start) replicate id
attribute	sample	long_name	String	Sample
attribute	sample	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	sample	units	String	unitless
variable	fluor_t0		float
attribute	fluor_t0	_FillValue	float	NaN
attribute	fluor_t0	actual_range	float	-0.4, 654.83
attribute	fluor_t0	bcodmo_name	String	fluorescence
attribute	fluor_t0	description	String	fluorescence intensity at time 0
attribute	fluor_t0	long_name	String	Fluor T0
attribute	fluor_t0	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/
attribute	fluor_t0	units	String	relative fluorescence units
variable	fluor_t1		float
attribute	fluor_t1	_FillValue	float	NaN
attribute	fluor_t1	actual_range	float	-0.83, 1099.15
attribute	fluor_t1	bcodmo_name	String	fluorescence
attribute	fluor_t1	description	String	fluorescence intensity at time 1
attribute	fluor_t1	long_name	String	Fluor T1
attribute	fluor_t1	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/
attribute	fluor_t1	units	String	relative fluorescence units
variable	fluor_t2		float
attribute	fluor_t2	_FillValue	float	NaN
attribute	fluor_t2	actual_range	float	-0.76, 1746.21
attribute	fluor_t2	bcodmo_name	String	fluorescence
attribute	fluor_t2	description	String	fluorescence intensity at time 2
attribute	fluor_t2	long_name	String	Fluor T2
attribute	fluor_t2	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/
attribute	fluor_t2	units	String	relative fluorescence units
variable	fluor_t3		float
attribute	fluor_t3	_FillValue	float	NaN
attribute	fluor_t3	actual_range	float	-0.16, 2074.2
attribute	fluor_t3	bcodmo_name	String	fluorescence
attribute	fluor_t3	description	String	fluorescence intensity at time 3
attribute	fluor_t3	long_name	String	Fluor T3
attribute	fluor_t3	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/
attribute	fluor_t3	units	String	relative fluorescence units
variable	time_t0		float
attribute	time_t0	_FillValue	float	NaN
attribute	time_t0	actual_range	float	-0.01, 0.05
attribute	time_t0	bcodmo_name	String	time_elapsed
attribute	time_t0	description	String	time since start of experiment; time point 0
attribute	time_t0	long_name	String	Time T0
attribute	time_t0	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/
attribute	time_t0	units	String	hours
variable	time_t1		float
attribute	time_t1	_FillValue	float	NaN
attribute	time_t1	actual_range	float	0.04, 3.38
attribute	time_t1	bcodmo_name	String	time_elapsed
attribute	time_t1	description	String	time elapsed from start of experiment; time point 1
attribute	time_t1	long_name	String	Time T1
attribute	time_t1	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/
attribute	time_t1	units	String	hours
variable	time_t2		float
attribute	time_t2	_FillValue	float	NaN
attribute	time_t2	actual_range	float	0.04, 5.83
attribute	time_t2	bcodmo_name	String	time_elapsed
attribute	time_t2	description	String	time elapsed from start of experiment; time point 2
attribute	time_t2	long_name	String	Time T2
attribute	time_t2	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/
attribute	time_t2	units	String	hours
variable	time_t3		float
attribute	time_t3	_FillValue	float	NaN
attribute	time_t3	actual_range	float	0.04, 7.93
attribute	time_t3	bcodmo_name	String	time_elapsed
attribute	time_t3	description	String	time elapsed from start of experiment; time point 3
attribute	time_t3	long_name	String	Time T3
attribute	time_t3	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/
attribute	time_t3	units	String	hours
variable	enz_activity_t0		float
attribute	enz_activity_t0	_FillValue	float	NaN
attribute	enz_activity_t0	actual_range	float	0.0, 801.19
attribute	enz_activity_t0	bcodmo_name	String	unknown
attribute	enz_activity_t0	description	String	enzymatic activity at time 0
attribute	enz_activity_t0	long_name	String	Enz Activity T0
attribute	enz_activity_t0	units	String	nanoMol/hour
variable	enz_activity_t1		float
attribute	enz_activity_t1	_FillValue	float	NaN
attribute	enz_activity_t1	actual_range	float	-0.01, 1340.68
attribute	enz_activity_t1	bcodmo_name	String	unknown
attribute	enz_activity_t1	description	String	enzymatic activity at time 1
attribute	enz_activity_t1	long_name	String	Enz Activity T1
attribute	enz_activity_t1	units	String	nanoMol/hour
variable	enz_activity_t2		float
attribute	enz_activity_t2	_FillValue	float	NaN
attribute	enz_activity_t2	actual_range	float	-0.01, 2126.34
attribute	enz_activity_t2	bcodmo_name	String	unknown
attribute	enz_activity_t2	description	String	enzymatic activity at time 2
attribute	enz_activity_t2	long_name	String	Enz Activity T2
attribute	enz_activity_t2	units	String	nanoMol/hour
variable	enz_activity_t3		float
attribute	enz_activity_t3	_FillValue	float	NaN
attribute	enz_activity_t3	actual_range	float	0.0, 2524.58
attribute	enz_activity_t3	bcodmo_name	String	unknown
attribute	enz_activity_t3	description	String	enzymatic activity at time 3
attribute	enz_activity_t3	long_name	String	Enz Activity T3
attribute	enz_activity_t3	units	String	nanoMol/hour
variable	SLOPE		float
attribute	SLOPE	_FillValue	float	NaN
attribute	SLOPE	actual_range	float	-0.001, 269.15
attribute	SLOPE	bcodmo_name	String	unknown
attribute	SLOPE	description	String	the slope of the graph of fluorescence intensity vs substrate concentration
attribute	SLOPE	long_name	String	SLOPE
attribute	SLOPE	units	String	unitless
variable	RSQR		float
attribute	RSQR	_FillValue	float	NaN
attribute	RSQR	actual_range	float	0.0, 1.0
attribute	RSQR	bcodmo_name	String	unknown
attribute	RSQR	description	String	the square of the correlation coefficient of fluorescence intensity vs substrate concentration
attribute	RSQR	long_name	String	RSQR
attribute	RSQR	units	String	unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.