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Title Sum-
Institution Dataset ID
     data   graph     files  public Images of particles collected in sediment traps for quantitative analysis from multiple
platforms from 2016-2017
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson,.odvTxt
attribute NC_GLOBAL acquisition_description String The NBST carried 4 collection tubes with a diameter of 12 cm (Valdes and Price
2000). The STST included 5 frames (KC Denmark) clipped onto a surface-
tethered, free drifting array line at increasing depths and each frame carried
4, 7 cm diameter collection tubes. The WW trap consisted of one, 4-tube trap
frame (KC Denmark) tethered by a bungee below the profiling component of the
WW array.\u00a0 To prepare tubes for deployment, seawater was collected from a
depth of 150 m using a CTD rosette and pumped through a 1 \u03bcm filter
cartridge. Trap tubes were filled with filtered water overlying a jar
containing a polyacrylamide gel layer (Durkin et al. 2015). Trap platforms
were deployed for between 1 day and 3.5 days (see Trap Deployment Log).\u00a0
Identically prepared tubes were incubated in parallel onboard the ship to
serve as process blanks.

Upon recovery, collection tubes were allowed to settle for at least 1 hour
before the overlying water was siphoned off.\u00a0 Jars containing
polyacrylamide gel were removed from trap tubes and the remaining overlying
water was carefully pipetted off the gel.\u00a0 Gels were stored at 4 degrees
C and imaged within the following 2 days before being stored at -80 degrees

Polyacrylamide gel layers were imaged on a dissecting microscope (Olympus
SZX16) with either a Luminera Infinity 2 (FK170124) or an Allied Vision
Technologies StingRay (EN572 and EN581) camera attachment.\u00a0 Particles
collected in gel layers during EN572 and EN581 were imaged under brightfield
illumination.\u00a0 Particles collected in gel layers during FK170124 were
imaged under both brightfield and oblique illumination, producing two separate
sets of images for each sample.\u00a0 EN572 gel layers were imaged with a
transparent grid to assist in tracking gel location during imaging. The grid
was not used when imaging samples collected during subsequent cruises because
the pronounced grid lines complicated image analysis. All gel layers were
imaged at 4 increasing magnifications, though the combination of
magnifications varied by cruise: at 7x, 20x, 40x, and 115x for EN572 samples,
at 7x, 20x, 40x, and 80x for EN581 samples, and at 7x, 20x, 50x, and 115x for
FK170124 samples. At magnifications greater than 7x, multiple focal planes
within a field of view were imaged to capture particles embedded in different
depths of the gel layer.\u00a0 The number of focal planes imaged was
consistent across all fields of view for a given magnification but varied
across cruises due to variation in gel thickness and particle types present.
To determine whether measured particle properties changed if gel layers are
frozen, samples collected during FK170124 were thawed after being stored for
approximately 1 year at -80\u00a0 degrees C and imaged again under both
brightfield and oblique illumination.
attribute NC_GLOBAL awards_0_award_nid String 675295
attribute NC_GLOBAL awards_0_award_number String OCE-1703664
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1703664 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Images of particles collected in sediment traps for quantitative analysis from multiple platforms from 2016-2017
PI: Colleen Durkin
Version: 2019-10-18
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL dataset_current_state String Final and no updates
attribute NC_GLOBAL date_created String 2018-11-07T17:55:41Z
attribute NC_GLOBAL date_modified String 2020-07-15T15:47:38Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.749412.1
attribute NC_GLOBAL Easternmost_Easting double 151.903
attribute NC_GLOBAL geospatial_lat_max double 39.88133333
attribute NC_GLOBAL geospatial_lat_min double 22.329
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double 151.903
attribute NC_GLOBAL geospatial_lon_min double -71.09866667
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/749412 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String camera
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Polyacrylamide gel layers were imaged on a dissecting microscope (Olympus SZX16) with either a Luminera Infinity 2 (FK170124) or an Allied Vision Technologies StingRay (EN572 and EN581) camera attachment.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 749428
attribute NC_GLOBAL instruments_0_description String All types of photographic equipment including stills, video, film and digital systems.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/311/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Camera
attribute NC_GLOBAL instruments_0_instrument_nid String 520
attribute NC_GLOBAL instruments_0_supplied_name String Luminera Infinity 2 microscope camera
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Polyacrylamide gel layers were imaged on a dissecting microscope (Olympus SZX16)
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 749427
attribute NC_GLOBAL instruments_1_description String Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB05/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Microscope-Optical
attribute NC_GLOBAL instruments_1_instrument_nid String 708
attribute NC_GLOBAL instruments_1_supplied_name String Olympus SZX16 Stereomicroscope
attribute NC_GLOBAL keywords String additional, Additional_Trap_Label, bco, bco-dmo, biological, chemical, cruise, data, dataset, date, days, deploy, Deploy_Date_UTC, Deploy_Time_UTC, deployment, Deployment_Duration_days, depth, dmo, duration, erddap, label, latitude, longitude, management, oceanography, office, platform, preliminary, recover, recover_date_time, Recover_Date_UTC, Recover_Latitude, Recover_Longitude, Recover_Time_UTC, time, trap, Trap_Platform
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/749412/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/749412 (external link)
attribute NC_GLOBAL Northernmost_Northing double 39.88133333
attribute NC_GLOBAL param_mapping String {'749412': {'Deploy_Latitude': 'flag - latitude', 'deploy_date_time': 'flag - time', 'Deploy_Longitude': 'flag - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/749412/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Moss Landing Marine Laboratories
attribute NC_GLOBAL people_0_affiliation_acronym String MLML
attribute NC_GLOBAL people_0_person_name String Colleen Durkin
attribute NC_GLOBAL people_0_person_nid String 675299
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Skidmore College
attribute NC_GLOBAL people_1_person_name String Margaret L. Estapa
attribute NC_GLOBAL people_1_person_nid String 644830
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of Rhode Island
attribute NC_GLOBAL people_2_affiliation_acronym String URI-GSO
attribute NC_GLOBAL people_2_person_name String Melissa Omand
attribute NC_GLOBAL people_2_person_nid String 675309
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Mathew Biddle
attribute NC_GLOBAL people_3_person_nid String 708682
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String EAGER DNA BioPump
attribute NC_GLOBAL projects_0_acronym String EAGER DNA BioPump
attribute NC_GLOBAL projects_0_description String Text from the NSF award abstract:
Carbon is fixed into organic matter by phytoplankton growing in the surface ocean, and is naturally sequestered in the ocean interior when particles and organisms sink: a process called the "biological pump." Because of its recognized influence on the global carbon cycle, ocean scientists have studied the biological pump for decades. However, we still do not have a sufficient understanding of the underlying processes to accurately quantify and predict carbon cycling. Much of this uncertainty stems from an inability to directly link specific plankton in the surface ocean with the types of particles sinking out of the surface ocean. To address this missing link in biological pump research, this work will directly observe how plankton are transported out of the surface ocean using novel, particle-specific observational approaches embedded within an interdisciplinary field program that will finely resolve upper ocean plankton groups and the resulting amount of sinking carbon across space and in time. The genetic identity of organisms within different types of sinking particles will be determined by sequencing the genetic contents of individually collected particles. This new application of a molecular method will definitively link surface plankton with sinking particles at five locations across the Pacific Ocean. This work has the potential to transform our understanding of the biological pump by identifying previously unknown links between surface ecosystems and sinking carbon particles. Because this work is embedded within an interdisciplinary field program, including biogeochemical modelers and remote sensing scientists, these data will feed directly into new models of the biological pump, improving our ability to quantify and predict carbon uptake by the ocean. This project will train 1 graduate student and at least 2 undergraduate researchers. Findings will be communicated to the non-scientific public through blogs, videos, and the public communication channels of participating institutions.
Accurate prediction of the global carbon cycle requires an understanding of the specific processes that link surface plankton communities and sinking particulate carbon flux (export) out of the surface ocean, but current methodological paradigms in biological pump research do not directly observe these processes. This project will comprehensively determine who is exported from the surface ocean and how using new, particle-resolving optical and molecular techniques embedded within a sampling scheme that characterizes export events at high time and space resolution. The investigation suggests that different plankton types in the surface waters are transported out of the surface ocean by distinct export pathways, and that an understanding of these connections is critical knowledge for global carbon cycle modeling. If successful, this work has the potential to transform our conceptual understanding of the biological pump by directly identifying mechanisms that link surface plankton with particle export, without relying on bulk sampling schemes and large-scale correlation analysis. Particle export environments will be studied at five open ocean locations during a cruise from Hawaii to Seattle in January-February 2017. The surface plankton communities will be characterized by a combination of satellite observations, sensors attached to a free-drifting, continuously profiling WireWalker, an in situ holographic camera, microscopy, and by sequencing 18S and 16S rRNA gene fragments. Exported particles will simultaneously be captured by various specialized sediment traps and their characteristics will be directly related to their sources in the surface community by identifying the genetic contents of individual particle types. Individual particles will be isolated from gel layers and the 16S and 18S rRNA gene fragments will be amplified and sequenced. This work would, for the first time, combine molecular approaches with particle-specific observations to enable simultaneous identification of both which organisms are exported and the processes responsible for their export.
attribute NC_GLOBAL projects_0_end_date String 2018-11
attribute NC_GLOBAL projects_0_geolocation String Eastern Pacific
attribute NC_GLOBAL projects_0_name String Collaborative Research: EAGER: Particle-specific DNA sequencing to directly observe ecological mechanisms of the biological pump
attribute NC_GLOBAL projects_0_project_nid String 675296
attribute NC_GLOBAL projects_0_start_date String 2016-12
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 22.329
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Images of particles collected in sediment traps for quantitative analysis from multiple platforms from 2016-2017
attribute NC_GLOBAL time_coverage_end String 2017-02-13T17:48Z
attribute NC_GLOBAL time_coverage_start String 2015-11-04T11:03Z
attribute NC_GLOBAL title String Images of particles collected in sediment traps for quantitative analysis from multiple platforms from 2016-2017
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -71.09866667
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5
variable Cruise   String  
attribute Cruise bcodmo_name String cruise_id
attribute Cruise description String Cruise identifier
attribute Cruise long_name String Cruise
attribute Cruise units String unitless
variable Trap_Platform   String  
attribute Trap_Platform bcodmo_name String platform
attribute Trap_Platform description String trap identifier
attribute Trap_Platform long_name String Trap Platform
attribute Trap_Platform units String unitless
variable Additional_Trap_Label   String  
attribute Additional_Trap_Label bcodmo_name String unknown
attribute Additional_Trap_Label description String additional trap label
attribute Additional_Trap_Label long_name String Additional Trap Label
attribute Additional_Trap_Label units String unitless
variable Depth   short  
attribute Depth _FillValue short 32767
attribute Depth actual_range short 50, 200
attribute Depth bcodmo_name String depth_trap
attribute Depth colorBarMaximum double 8000.0
attribute Depth colorBarMinimum double -8000.0
attribute Depth colorBarPalette String TopographyDepth
attribute Depth description String depth of trap
attribute Depth long_name String Depth
attribute Depth standard_name String depth
attribute Depth units String meters
variable Deployment_Duration_days   float  
attribute Deployment_Duration_days _FillValue float NaN
attribute Deployment_Duration_days actual_range float 1.042, 3.41
attribute Deployment_Duration_days bcodmo_name String duration
attribute Deployment_Duration_days description String deployment duration
attribute Deployment_Duration_days long_name String Deployment Duration Days
attribute Deployment_Duration_days units String days
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 22.329, 39.88133333
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String deployment latitude with positive values indicating North
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude source_name String Deploy_Latitude
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -71.09866667, 151.903
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String deployment longitude with negative values indicating West
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude source_name String Deploy_Longitude
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable Deploy_Date_UTC   String  
attribute Deploy_Date_UTC bcodmo_name String date
attribute Deploy_Date_UTC description String date of deployment in UTC following ISO-8601 convention
attribute Deploy_Date_UTC long_name String Deploy Date UTC
attribute Deploy_Date_UTC nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute Deploy_Date_UTC units String unitless
variable Deploy_Time_UTC   String  
attribute Deploy_Time_UTC bcodmo_name String time
attribute Deploy_Time_UTC description String Time of deployment in UTC following ISO-8601 convention
attribute Deploy_Time_UTC long_name String Deploy Time UTC
attribute Deploy_Time_UTC nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/AHMSAA01/ (external link)
attribute Deploy_Time_UTC units String unitless
variable Recover_Latitude   double  
attribute Recover_Latitude _FillValue double NaN
attribute Recover_Latitude actual_range double 21.592, 39.94433333
attribute Recover_Latitude bcodmo_name String latitude
attribute Recover_Latitude colorBarMaximum double 90.0
attribute Recover_Latitude colorBarMinimum double -90.0
attribute Recover_Latitude description String recover latitude with positive values indicating North
attribute Recover_Latitude long_name String Latitude
attribute Recover_Latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute Recover_Latitude standard_name String latitude
attribute Recover_Latitude units String decimal degrees
variable Recover_Longitude   double  
attribute Recover_Longitude _FillValue double NaN
attribute Recover_Longitude actual_range double -71.092106, 151.779
attribute Recover_Longitude bcodmo_name String longitude
attribute Recover_Longitude colorBarMaximum double 180.0
attribute Recover_Longitude colorBarMinimum double -180.0
attribute Recover_Longitude description String recover longitude with negative values indicating West
attribute Recover_Longitude long_name String Longitude
attribute Recover_Longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute Recover_Longitude standard_name String longitude
attribute Recover_Longitude units String decimal degrees
variable Recover_Date_UTC   String  
attribute Recover_Date_UTC bcodmo_name String date
attribute Recover_Date_UTC description String date of recover in UTC following ISO-8601 convention
attribute Recover_Date_UTC long_name String Recover Date UTC
attribute Recover_Date_UTC nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute Recover_Date_UTC units String unitless
variable Recover_Time_UTC   String  
attribute Recover_Time_UTC bcodmo_name String time
attribute Recover_Time_UTC description String Time of recover in UTC following ISO-8601 convention
attribute Recover_Time_UTC long_name String Recover Time UTC
attribute Recover_Time_UTC nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/AHMSAA01/ (external link)
attribute Recover_Time_UTC units String unitless
variable time   double  
attribute time _CoordinateAxisType String Time
attribute time actual_range double 1.44663498E9, 1.48700808E9
attribute time axis String T
attribute time bcodmo_name String ISO_DateTime_UTC
attribute time description String deployment date and time following ISO-8901 convention
attribute time ioos_category String Time
attribute time long_name String Deploy Date Time
attribute time nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/ (external link)
attribute time source_name String deploy_date_time
attribute time standard_name String time
attribute time time_origin String 01-JAN-1970 00:00:00
attribute time time_precision String 1970-01-01T00:00Z
attribute time units String seconds since 1970-01-01T00:00:00Z
variable recover_date_time   String  
attribute recover_date_time bcodmo_name String ISO_DateTime_UTC
attribute recover_date_time description String recover date and time following ISO-8901 convention
attribute recover_date_time long_name String Recover Date Time
attribute recover_date_time nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/ (external link)
attribute recover_date_time time_precision String 1970-01-01T00:00Z
attribute recover_date_time units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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