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Title Sum-
Institution Dataset ID
     data   graph     files  public Data from an experiment that measured the occurrence of feeding among 8 Prorocentrum minimum
strains on fluorescently labeled bacteria or the cryptophyte Teleaulax amphioxeia
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Prorocentrum minimum culturing: All cultures were maintained routinely in
F/2-Si in 32 PSU seawater, at 18C and 14:10 light:dark cycle at 50 uE (u =
micro). All cultures were transferred once every two weeks.

At each time point, 2 ml of cells were removed from experimental culture
flasks and preserved with gluteraldehyde (1% final concentration) and stored
at 4C until used to make microscopy slides. To make slides, 1 ml of preserved
sample was filtered onto a black 2 um nucleopore polycarbonate filter, and
then mounted on a glass microscope slide with fluorescence grade immersion
oil. Slides were then counted using fluorescence microscopy\u00a0and stored at

Culturing and experimental methods can be found in Johnson 2015.
attribute NC_GLOBAL awards_0_award_nid String 615829
attribute NC_GLOBAL awards_0_award_number String OCE-1436169
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1436169 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Pmin strain feeding 2
Data from experiment measuring feeding among 8 Prorocentrum minimum
strains on fluorescently labeled bacteria or the cryptophyte Teleaulax amphioxeia
PI: Matthew D. Johnson (WHOI)
Version date: 06 December 2018
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2018-12-04T21:37:45Z
attribute NC_GLOBAL date_modified String 2019-03-15T15:32:48Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.750823.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/750823 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 750828
attribute NC_GLOBAL instruments_0_description String Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB06/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Microscope-Fluorescence
attribute NC_GLOBAL instruments_0_instrument_nid String 695
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, cell, cells, cells_feeding, chemical, data, dataset, dmo, erddap, feeding, gfi, GFI_per_cell, management, oceanography, office, ofi, OFI_per_cell, pcnt, pcnt_cells_feeding, per, preliminary, prey, rep, strain, treatment
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/750823/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/750823 (external link)
attribute NC_GLOBAL param_mapping String {'750823': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/750823/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_0_affiliation_acronym String WHOI
attribute NC_GLOBAL people_0_person_name String Matthew D. Johnson
attribute NC_GLOBAL people_0_person_nid String 51232
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Shannon Rauch
attribute NC_GLOBAL people_1_person_nid String 51498
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String Mixo Foodwebs
attribute NC_GLOBAL projects_0_acronym String Mixo Foodwebs
attribute NC_GLOBAL projects_0_description String Marine phytoplankton are responsible for about half of global primary production despite being seasonally or chronically nutrient limited. To cope with this, many phytoplankton supplement their nutritional needs through mixotrophy, which involves feeding on bacteria or other algae. These microscopic Venus Fly Traps of the ocean are major players in marine microbial food webs, yet we know so little about when they feed and how their eating is balanced with photosynthesis. This research will shed light on how environmental and cellular factors control mixotrophy, and how mixotrophy and photosynthesis are integrated in the overall metabolism. While understanding the ecological role of mixotrophy in ocean food webs is center to this work, results from this study will also shed light on the evolution of mixotrophy by identifying potential tradeoffs between feeding and photosynthesis.
Mixotrophy refers to species that combine some level of phagotrophy and phototrophy, and represents a diverse array of ecological interactions and cellular and metabolic adaptations. While often perceived as an exception to the norm, mixotrophy is commonplace in marine food webs, affording phytoplankton greater ecological fitness during periods of low or limiting nutrients while stabilizing food webs. Many mixotrophs have a low chlorophyll: carbon ratio, which tends to make them poor phototrophic competitors. In turn, feeding allows these species to achieve maximum growth while in some cases also eliminating their competitors. Other mixotrophs are strong phototrophic competitors, and only feed when severely nutrient limited. This research will determine the cellular and environmental factors that lead to feeding by marine phytoplankton, and how the contrasting metabolisms of heterotrophy and photosynthesis are integrated within a cell. This research will involve laboratory-based experiments on model dinoflagellate and chrysophyte cultures. Using microscopy, physiology, proteomics and metabolomics approaches, this work will test hypotheses about the ultimate causes and consequences of mixotrophy. The major objectives are to determine 1) environmental controls for inducing mixotrophy, 2) the role of prey quality on predator selection, 3) cellular and molecular controls of mixotrophy, and 4) nutrient assimilation and integrated metabolism. Using these various research approaches, this work will produce a comprehensive view of several mixotrophs and provide new insights into cellular, ecological, and evolutionary aspects of mixotrophy. Results from this research will improve our understanding of the physiological and ecological role of mixotrophy in marine phytoplankton, and provide much needed molecular markers for studying this process in both the laboratory and field.
attribute NC_GLOBAL projects_0_end_date String 2017-09
attribute NC_GLOBAL projects_0_geolocation String laboratory: Woods Hole, Mass. USA
attribute NC_GLOBAL projects_0_name String Exploring the physiological and ecological basis of mixotrophy in marine food webs
attribute NC_GLOBAL projects_0_project_nid String 615830
attribute NC_GLOBAL projects_0_start_date String 2014-10
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String This dataset contains data from an experiment that measured the occurrence of feeding among 8 Prorocentrum minimum strains on fluorescently labeled bacteria or the cryptophyte Teleaulax amphioxeia.
attribute NC_GLOBAL title String Data from an experiment that measured the occurrence of feeding among 8 Prorocentrum minimum strains on fluorescently labeled bacteria or the cryptophyte Teleaulax amphioxeia
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Prey   String  
attribute Prey bcodmo_name String treatment
attribute Prey description String Either fluorescently labeled bacteria (FLB) or the cryptophyte Teleaulax amphioxeia (CRYPT) was used
attribute Prey long_name String Prey
attribute Prey units String unitless
variable Strain   String  
attribute Strain bcodmo_name String sample
attribute Strain description String Prorocentrum minimum culture strain name
attribute Strain long_name String Strain
attribute Strain nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Strain units String unitless
variable treatment   String  
attribute treatment bcodmo_name String treatment
attribute treatment description String Treatments are as follows: LOG1 is 3 days growing in F/2 in log phase; LOG2 is 7 days growing in F/2 in log phase; STAT1 is 14 days growing in F/2 in stationary phase; STAT2 is 21 days growing in F/2 in stationary phase; P1 is 3 days of growing under phosphorous starvation (F/2-P); P2 is 7 days of growing under phosphorous starvation (F/2-P); P3 is 14 days of growing under phosphorous starvation (F/2-P); P4 is 21 days of growing under phosphorous starvation (F/2-P)
attribute treatment long_name String Treatment
attribute treatment units String unitless
variable rep   byte  
attribute rep _FillValue byte 127
attribute rep actual_range byte 1, 2
attribute rep bcodmo_name String replicate
attribute rep description String Replicate for each strain (treatment) n=3
attribute rep long_name String Rep
attribute rep units String unitless
variable cells   short  
attribute cells _FillValue short 32767
attribute cells actual_range short 93, 561
attribute cells bcodmo_name String count
attribute cells description String Number of P. minimum cells counted
attribute cells long_name String Cells
attribute cells units String unitless
variable cells_feeding   byte  
attribute cells_feeding _FillValue byte 127
attribute cells_feeding actual_range byte 0, 113
attribute cells_feeding bcodmo_name String count
attribute cells_feeding description String Number of P. minimum cells with ingested fluorescently labeled prey
attribute cells_feeding long_name String Cells Feeding
attribute cells_feeding units String unitless
variable pcnt_cells_feeding   float  
attribute pcnt_cells_feeding _FillValue float NaN
attribute pcnt_cells_feeding actual_range float 0.0, 70.8
attribute pcnt_cells_feeding bcodmo_name String count
attribute pcnt_cells_feeding description String The percentage of cells feeding
attribute pcnt_cells_feeding long_name String Pcnt Cells Feeding
attribute pcnt_cells_feeding units String unitless
variable GFI   float  
attribute GFI _FillValue float NaN
attribute GFI actual_range float 0.0, 307.0
attribute GFI bcodmo_name String count
attribute GFI description String Number of green fluorescent inclusions (GFIs); GFIs are food vacuoles of ingested FLB
attribute GFI long_name String GFI
attribute GFI units String unitless
variable GFI_per_cell   float  
attribute GFI_per_cell _FillValue float NaN
attribute GFI_per_cell actual_range float 0.0, 1.91
attribute GFI_per_cell bcodmo_name String count
attribute GFI_per_cell description String Number of GFIs per P. minimum cell
attribute GFI_per_cell long_name String GFI Per Cell
attribute GFI_per_cell units String unitless
variable OFI   float  
attribute OFI _FillValue float NaN
attribute OFI actual_range float 0.0, 240.0
attribute OFI bcodmo_name String count
attribute OFI description String Number of orange fluorescent inclusions (OFIs); OFIs are food vacuoles from ingesting phycoerythrin-containing cryptophyte prey
attribute OFI long_name String OFI
attribute OFI units String unitless
variable OFI_per_cell   float  
attribute OFI_per_cell _FillValue float NaN
attribute OFI_per_cell actual_range float 0.0, 2.39
attribute OFI_per_cell bcodmo_name String count
attribute OFI_per_cell description String Number of OFIs per P. minimum cell
attribute OFI_per_cell long_name String OFI Per Cell
attribute OFI_per_cell units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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