ERDDAP > info > bcodmo_dataset_750823

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[Pmin strain feeding 2] - Data from an experiment that measured the occurrence of feeding among 8 Prorocentrum minimum strains on fluorescently labeled bacteria or the cryptophyte Teleaulax amphioxeia (Exploring the physiological and ecological basis of mixotrophy in marine food webs)		I   M	background			BCO-DMO	bcodmo_dataset_750823

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Prorocentrum minimum culturing: All cultures were maintained routinely in F/2-Si in 32 PSU seawater, at 18C and 14:10 light:dark cycle at 50 uE (u = micro). All cultures were transferred once every two weeks. At each time point, 2 ml of cells were removed from experimental culture flasks and preserved with gluteraldehyde (1% final concentration) and stored at 4C until used to make microscopy slides. To make slides, 1 ml of preserved sample was filtered onto a black 2 um nucleopore polycarbonate filter, and then mounted on a glass microscope slide with fluorescence grade immersion oil. Slides were then counted using fluorescence microscopy\u00a0and stored at -20C. Culturing and experimental methods can be found in Johnson 2015.
attribute	NC_GLOBAL	awards_0_award_nid	String	615829
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1436169
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1436169
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Michael E. Sieracki
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50446
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Pmin strain feeding 2 Data from experiment measuring feeding among 8 Prorocentrum minimum strains on fluorescently labeled bacteria or the cryptophyte Teleaulax amphioxeia PI: Matthew D. Johnson (WHOI) Version date: 06 December 2018
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2018-12-04T21:37:45Z
attribute	NC_GLOBAL	date_modified	String	2019-03-15T15:32:48Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.750823.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/750823
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	750828
attribute	NC_GLOBAL	instruments_0_description	String	Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB06/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Microscope-Fluorescence
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	695
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, cell, cells, cells_feeding, chemical, data, dataset, dmo, erddap, feeding, gfi, GFI_per_cell, management, oceanography, office, ofi, OFI_per_cell, pcnt, pcnt_cells_feeding, per, preliminary, prey, rep, strain, treatment
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/750823/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/750823
attribute	NC_GLOBAL	param_mapping	String	{'750823': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/750823/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	WHOI
attribute	NC_GLOBAL	people_0_person_name	String	Matthew D. Johnson
attribute	NC_GLOBAL	people_0_person_nid	String	51232
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Shannon Rauch
attribute	NC_GLOBAL	people_1_person_nid	String	51498
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	Mixo Foodwebs
attribute	NC_GLOBAL	projects_0_acronym	String	Mixo Foodwebs
attribute	NC_GLOBAL	projects_0_description	String	Marine phytoplankton are responsible for about half of global primary production despite being seasonally or chronically nutrient limited. To cope with this, many phytoplankton supplement their nutritional needs through mixotrophy, which involves feeding on bacteria or other algae. These microscopic Venus Fly Traps of the ocean are major players in marine microbial food webs, yet we know so little about when they feed and how their eating is balanced with photosynthesis. This research will shed light on how environmental and cellular factors control mixotrophy, and how mixotrophy and photosynthesis are integrated in the overall metabolism. While understanding the ecological role of mixotrophy in ocean food webs is center to this work, results from this study will also shed light on the evolution of mixotrophy by identifying potential tradeoffs between feeding and photosynthesis. Mixotrophy refers to species that combine some level of phagotrophy and phototrophy, and represents a diverse array of ecological interactions and cellular and metabolic adaptations. While often perceived as an exception to the norm, mixotrophy is commonplace in marine food webs, affording phytoplankton greater ecological fitness during periods of low or limiting nutrients while stabilizing food webs. Many mixotrophs have a low chlorophyll: carbon ratio, which tends to make them poor phototrophic competitors. In turn, feeding allows these species to achieve maximum growth while in some cases also eliminating their competitors. Other mixotrophs are strong phototrophic competitors, and only feed when severely nutrient limited. This research will determine the cellular and environmental factors that lead to feeding by marine phytoplankton, and how the contrasting metabolisms of heterotrophy and photosynthesis are integrated within a cell. This research will involve laboratory-based experiments on model dinoflagellate and chrysophyte cultures. Using microscopy, physiology, proteomics and metabolomics approaches, this work will test hypotheses about the ultimate causes and consequences of mixotrophy. The major objectives are to determine 1) environmental controls for inducing mixotrophy, 2) the role of prey quality on predator selection, 3) cellular and molecular controls of mixotrophy, and 4) nutrient assimilation and integrated metabolism. Using these various research approaches, this work will produce a comprehensive view of several mixotrophs and provide new insights into cellular, ecological, and evolutionary aspects of mixotrophy. Results from this research will improve our understanding of the physiological and ecological role of mixotrophy in marine phytoplankton, and provide much needed molecular markers for studying this process in both the laboratory and field.
attribute	NC_GLOBAL	projects_0_end_date	String	2017-09
attribute	NC_GLOBAL	projects_0_geolocation	String	laboratory: Woods Hole, Mass. USA
attribute	NC_GLOBAL	projects_0_name	String	Exploring the physiological and ecological basis of mixotrophy in marine food webs
attribute	NC_GLOBAL	projects_0_project_nid	String	615830
attribute	NC_GLOBAL	projects_0_start_date	String	2014-10
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	This dataset contains data from an experiment that measured the occurrence of feeding among 8 Prorocentrum minimum strains on fluorescently labeled bacteria or the cryptophyte Teleaulax amphioxeia.
attribute	NC_GLOBAL	title	String	[Pmin strain feeding 2] - Data from an experiment that measured the occurrence of feeding among 8 Prorocentrum minimum strains on fluorescently labeled bacteria or the cryptophyte Teleaulax amphioxeia (Exploring the physiological and ecological basis of mixotrophy in marine food webs)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Prey		String
attribute	Prey	bcodmo_name	String	treatment
attribute	Prey	description	String	Either fluorescently labeled bacteria (FLB) or the cryptophyte Teleaulax amphioxeia (CRYPT) was used
attribute	Prey	long_name	String	Prey
attribute	Prey	units	String	unitless
variable	Strain		String
attribute	Strain	bcodmo_name	String	sample
attribute	Strain	description	String	Prorocentrum minimum culture strain name
attribute	Strain	long_name	String	Strain
attribute	Strain	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	Strain	units	String	unitless
variable	treatment		String
attribute	treatment	bcodmo_name	String	treatment
attribute	treatment	description	String	Treatments are as follows: LOG1 is 3 days growing in F/2 in log phase; LOG2 is 7 days growing in F/2 in log phase; STAT1 is 14 days growing in F/2 in stationary phase; STAT2 is 21 days growing in F/2 in stationary phase; P1 is 3 days of growing under phosphorous starvation (F/2-P); P2 is 7 days of growing under phosphorous starvation (F/2-P); P3 is 14 days of growing under phosphorous starvation (F/2-P); P4 is 21 days of growing under phosphorous starvation (F/2-P)
attribute	treatment	long_name	String	Treatment
attribute	treatment	units	String	unitless
variable	rep		byte
attribute	rep	_FillValue	byte	127
attribute	rep	actual_range	byte	1, 2
attribute	rep	bcodmo_name	String	replicate
attribute	rep	description	String	Replicate for each strain (treatment) n=3
attribute	rep	long_name	String	Rep
attribute	rep	units	String	unitless
variable	cells		short
attribute	cells	_FillValue	short	32767
attribute	cells	actual_range	short	93, 561
attribute	cells	bcodmo_name	String	count
attribute	cells	description	String	Number of P. minimum cells counted
attribute	cells	long_name	String	Cells
attribute	cells	units	String	unitless
variable	cells_feeding		byte
attribute	cells_feeding	_FillValue	byte	127
attribute	cells_feeding	actual_range	byte	0, 113
attribute	cells_feeding	bcodmo_name	String	count
attribute	cells_feeding	description	String	Number of P. minimum cells with ingested fluorescently labeled prey
attribute	cells_feeding	long_name	String	Cells Feeding
attribute	cells_feeding	units	String	unitless
variable	pcnt_cells_feeding		float
attribute	pcnt_cells_feeding	_FillValue	float	NaN
attribute	pcnt_cells_feeding	actual_range	float	0.0, 70.8
attribute	pcnt_cells_feeding	bcodmo_name	String	count
attribute	pcnt_cells_feeding	description	String	The percentage of cells feeding
attribute	pcnt_cells_feeding	long_name	String	Pcnt Cells Feeding
attribute	pcnt_cells_feeding	units	String	unitless
variable	GFI		float
attribute	GFI	_FillValue	float	NaN
attribute	GFI	actual_range	float	0.0, 307.0
attribute	GFI	bcodmo_name	String	count
attribute	GFI	description	String	Number of green fluorescent inclusions (GFIs); GFIs are food vacuoles of ingested FLB
attribute	GFI	long_name	String	GFI
attribute	GFI	units	String	unitless
variable	GFI_per_cell		float
attribute	GFI_per_cell	_FillValue	float	NaN
attribute	GFI_per_cell	actual_range	float	0.0, 1.91
attribute	GFI_per_cell	bcodmo_name	String	count
attribute	GFI_per_cell	description	String	Number of GFIs per P. minimum cell
attribute	GFI_per_cell	long_name	String	GFI Per Cell
attribute	GFI_per_cell	units	String	unitless
variable	OFI		float
attribute	OFI	_FillValue	float	NaN
attribute	OFI	actual_range	float	0.0, 240.0
attribute	OFI	bcodmo_name	String	count
attribute	OFI	description	String	Number of orange fluorescent inclusions (OFIs); OFIs are food vacuoles from ingesting phycoerythrin-containing cryptophyte prey
attribute	OFI	long_name	String	OFI
attribute	OFI	units	String	unitless
variable	OFI_per_cell		float
attribute	OFI_per_cell	_FillValue	float	NaN
attribute	OFI_per_cell	actual_range	float	0.0, 2.39
attribute	OFI_per_cell	bcodmo_name	String	count
attribute	OFI_per_cell	description	String	Number of OFIs per P. minimum cell
attribute	OFI_per_cell	long_name	String	OFI Per Cell
attribute	OFI_per_cell	units	String	unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.