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     data   graph     files  public [Mytilus Elemental Fingerprints] - Elemental fingerprints of larval, juvenile, and settler
Mytilus collected in the Gulf of Maine between 2015 and 2017 (An integrated theoretical and
empirical approach to across-shelf mixing and connectivity of mussel populations)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String We quantified larval fingerprints during spawning at each of the potential
source populations to develop a reference map that will allow us to assign
settled mussels to natal sites based on the elemental fingerprints of their
larval shell. To develop a reference map of elemental signatures, we reared
larvae in situ at each major mussel population (see sample locations data).
After populations spawned in the field (based on our monitoring of gonad
indices in each population) adult mussels from multiple sites were collected,
spawned in the laboratory and the eggs fertilized. Within 24-48 hours (before
shell formation begins) larvae were placed into small chambers and moored in
situ at 3-5m depth. Because spawning varied among the populations, we
staggered our deployment of larvae to coincide with our estimates of spawning
times. After four weeks, larval mussels were removed from in situ chambers,
shells were rinsed in deionized water, dried and prepared for ICPMS. Settlers
were collected from settlement plates deployed at each population.

Elemental concentrations were determined from single spot ablation in larval
shells (85% energy, 10 hz, 200 shots, 25 \u03bcm spot diameter) for both
larvae and settlers. Elemental compositions were quantified by external
calibration (USGS MACS-3) and internal standardization. We quantified
elemental compositions from count per second (cps) measurements of 24Mg, 55Mn,
59Co, 88Sr, 138Ba, 139La, 208Pb and 46Ca using laser ablation inductively
coupled mass spectrometry (213 Nd:YAG laser coupled with Elan DRC II ICPMS) at
the University of Massachusetts, Boston (LA-ICPMS). Elemental abundances were
calculated as analyte cps/ 43Ca cps. We performed a one-point calibration
using gas blank and MACS-3, and ran MACS-1 as a check standard every ten
samples. Analytical accuracy and precision based on repeated check standard
analyses was 96 + 4.4% (mean + sd). Each sample was analyzed in triplicate and
mean concentrations normalized to calcium are reported.

Location:
Gulf of Maine: Frenchmen Bay (44 28.239 N -68 15.927 W) to Machais Bay (44
39.350 N -67 21.320 W).
attribute NC_GLOBAL awards_0_award_nid String 527081
attribute NC_GLOBAL awards_0_award_number String OCE-1334022
attribute NC_GLOBAL awards_0_data_url String http://nsf.gov/awardsearch/showAward?AWD_ID=1334022 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL awards_1_award_nid String 542418
attribute NC_GLOBAL awards_1_award_number String OCE-1458154
attribute NC_GLOBAL awards_1_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=1458154 (external link)
attribute NC_GLOBAL awards_1_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_1_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_1_funding_source_nid String 355
attribute NC_GLOBAL awards_1_program_manager String David L. Garrison
attribute NC_GLOBAL awards_1_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Mytilus fingerprints
PI: Ron Etter
Data Version 1: 2019-12-09
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2019-01-04T21:23:49Z
attribute NC_GLOBAL date_modified String 2019-12-20T16:34:45Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.752530.2
attribute NC_GLOBAL Easternmost_Easting double -66.97212
attribute NC_GLOBAL geospatial_lat_max double 44.832368
attribute NC_GLOBAL geospatial_lat_min double 42.31377
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -66.97212
attribute NC_GLOBAL geospatial_lon_min double -71.04074
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/752530 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Mass Spec
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Teledyne Cetac 213 G2+ laser coupled with a Perkin Elmer NexION 2000C mass spectrometer with autosampler and cross-flow nebulizer.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 763563
attribute NC_GLOBAL instruments_0_description String General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB16/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Mass Spectrometer
attribute NC_GLOBAL instruments_0_instrument_nid String 685
attribute NC_GLOBAL instruments_0_supplied_name String Perkin Elmer NexION 2000C
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Teledyne Cetac 213 G2+ laser coupled with a Perkin Elmer NexION 2000C mass spectrometer with autosampler and cross-flow nebulizer
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 763564
attribute NC_GLOBAL instruments_1_description String A device that generates an intense beam of coherent monochromatic light (or other electromagnetic radiation) by stimulated emission of photons from excited atoms or molecules.
attribute NC_GLOBAL instruments_1_instrument_name String Laser
attribute NC_GLOBAL instruments_1_instrument_nid String 564857
attribute NC_GLOBAL instruments_1_supplied_name String Teledyne Cetac 213 G2+
attribute NC_GLOBAL keywords String age, bco, bco-dmo, biological, chemical, code, data, dataset, date, dmo, erddap, latitude, longitude, management, number, oceanography, office, preliminary, sample, Sample_Number, site, Site_Code, time
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/752530/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/752530 (external link)
attribute NC_GLOBAL Northernmost_Northing double 44.832368
attribute NC_GLOBAL param_mapping String {'752530': {'Latitude': 'master - latitude', 'Longitude': 'master - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/752530/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Massachusetts Boston
attribute NC_GLOBAL people_0_affiliation_acronym String UMass Boston
attribute NC_GLOBAL people_0_person_name String Dr Ron J Etter
attribute NC_GLOBAL people_0_person_nid String 51359
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_1_person_name String Amber York
attribute NC_GLOBAL people_1_person_nid String 643627
attribute NC_GLOBAL people_1_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL project String MuLTI-2,GOMEPRO
attribute NC_GLOBAL projects_0_acronym String MuLTI-2
attribute NC_GLOBAL projects_0_description String Acronym "MuLTI-2" (Mussel Larval Transport Initiative-2)
Extracted from the NSF award abstract:
Existing larval transport models focus mainly on along-shelf transport and have done little to explicitly incorporate the effects of cross-shelf mixing and transport processes. Yet cross-shelf transits (both outgoing and incoming legs) are critical components of the dispersal paths of coastal invertebrates. This project will explore the role of cross-shelf mixing in the connectivity of blue mussel populations in eastern Maine. Previous work has shown that the Eastern Maine Coastal Current (EMCC) begins to diverge from shore southwest of the Grand Manan Channel and creates a gradient in cross-shelf mixing and larval transport, with cross-shelf mixing being more common on the northeastern end, episodic in the transitional middle area, and then becoming rare in the southwestern half of the region of the Gulf of Maine. As a result, the investigators predict that northeastern populations of mussels are seeded mostly from up-stream sources, while a significant component of self-seeding (local retention) exists in southwestern populations. Larvae settling in the intervening bays are expected to be derived from a mixture of local and up-stream sources. Using a combined empirical and theoretical approach hydrographic, current profile, and larval vertical migration data will be collected and used to develop and validate a high-resolution coastal circulation model coupled to a model of larval behavior. The investigators will model simulations in different years using the empirical data from mussel reproductive output and spawning times. Connectivity predicted from this model will be then tested against independent empirical estimates of connectivity based on trace element fingerprinting for larvae which can be connected to specific natal habitats. Regions of agreement and discrepancy in the model will be identified to guide additional data collection and model refinement. This iterative process will ensure an understanding of both larval transport patterns and processes, and provide estimates of inter-annual variability in connectivity for blue mussel populations in the Gulf of Maine.
attribute NC_GLOBAL projects_0_end_date String 2017-08
attribute NC_GLOBAL projects_0_geolocation String Gulf of Maine: Frenchmen Bay (44 28.239 N -68 15.927 W) to Machais Bay (44 39.350 N -67 21.320 W)
attribute NC_GLOBAL projects_0_name String An integrated theoretical and empirical approach to across-shelf mixing and connectivity of mussel populations
attribute NC_GLOBAL projects_0_project_nid String 527082
attribute NC_GLOBAL projects_0_start_date String 2013-09
attribute NC_GLOBAL projects_1_acronym String GOMEPRO
attribute NC_GLOBAL projects_1_description String Rocky intertidal habitats in the Gulf of Maine (GoM) provide a model system to examine the structure and dynamics of natural communities. Throughout the Gulf of Maine, the same species are often found in these habitats but community structure, dynamics and productivity differ markedly among 3 distinct regions (southern, central and northern GoM). Past influential work, conducted primarily in the southern and central GoM, focused on the local processes driving intertidal community structure but produced very different conceptual models of how these communities are structured. This project examines whether regional differences in rocky shore community processes are driven by differences in recruitment that are shaped by regional variation in temperature and food availability and nearshore coastal oceanography. This project will improve the understanding of how large-scale environmental forces interact with local processes to control the distribution of species and the structure and dynamics of these communities. Understanding the interaction between processes operating at different scales is fundamentally important to developing more reliable models that can be used to predict community dynamics. In addition, data resulting from this project will have important implications for regional dynamics in commercially important species and for ecosystem and fisheries management within the GoM.
The overarching hypothesis of this project is that regional differences in community-level processes are driven by very different patterns of population connectivity and recruitment in a few key species, and that these differences are ultimately caused by regional variation in temperature and food availability and mediated by physical larval transport processes. Hence, the project will test the following hypotheses with manipulative field experiments, field sampling, connectivity estimates, and integrative modeling:
1) Locally-dispersing species dominate dynamics in regions with a net export of planktonic larvae (Northern GoM), while species with planktonic larvae dominate the dynamics in regions with high settlement and extensive connectivity among populations (Southern GoM).
2) Settlement density of species with planktonic larvae increases from northern to southern regions in accord with regional variation in food availability.
3) Population connectivity varies greatly among regions, with regions differing in the degree to which they are self-seeded or serve as larval sources vs. sinks; self-seeding leads to relatively localized population dynamics in the middle portion of the GoM.
4) Patterns of population connectivity are driven by physical transport processes and can be represented by coupling basic larval behavior models with circulation models.
At 18 different sites in the GoM across ~ 600 km, surveys will evaluate variation in recruitment, food availability and secondary productivity and experiments will assess community processes in wave-exposed and sheltered habitats. We will use hydrographic, current profile, and larval vertical distribution surveys to collect data for coupled larval/circulation models. Population connectivity will be both modeled and empirically evaluated (for one species) using elemental fingerprinting. A spatially explicit metacommunity model will integrate across all project components and test the relative importance of regional and local processes in controlling community organization and dynamics.
attribute NC_GLOBAL projects_1_end_date String 2019-01
attribute NC_GLOBAL projects_1_geolocation String Rocky intertidal shores and nearshore coastal waters throughout the Gulf of Maine
attribute NC_GLOBAL projects_1_name String Intertidal community assembly and dynamics: Integrating broad-scale regional variation in environmental forcing and benthic-pelagic coupling
attribute NC_GLOBAL projects_1_project_nid String 542407
attribute NC_GLOBAL projects_1_start_date String 2015-02
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 42.31377
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Elemental fingerprints of larval, juvenile, and settler Mytilus collected in the Gulf of Maine between 2015 and 2017.
attribute NC_GLOBAL title String [Mytilus Elemental Fingerprints] - Elemental fingerprints of larval, juvenile, and settler Mytilus collected in the Gulf of Maine between 2015 and 2017 (An integrated theoretical and empirical approach to across-shelf mixing and connectivity of mussel populations)
attribute NC_GLOBAL version String 2
attribute NC_GLOBAL Westernmost_Easting double -71.04074
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Site   String  
attribute Site bcodmo_name String site
attribute Site description String Sample location
attribute Site long_name String Site
attribute Site units String unitless
variable Site_Code   String  
attribute Site_Code bcodmo_name String site_descrip
attribute Site_Code description String Sample location abbreviation
attribute Site_Code long_name String Site Code
attribute Site_Code units String unitless
variable Age   String  
attribute Age bcodmo_name String sample_descrip
attribute Age description String Age (Larvae, Juvenile or Settler)
attribute Age long_name String Age
attribute Age units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 42.31377, 44.832368
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -71.04074, -66.97212
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable Date   String  
attribute Date bcodmo_name String date_utc
attribute Date description String Sample collection date (UTC) in ISO 8601 format yyyy-MM-dd
attribute Date long_name String Date
attribute Date source_name String Date
attribute Date time_precision String 1970-01-01
attribute Date units String unitless
variable Sample_Number   byte  
attribute Sample_Number _FillValue byte 127
attribute Sample_Number actual_range byte 1, 119
attribute Sample_Number bcodmo_name String sample
attribute Sample_Number colorBarMaximum double 100.0
attribute Sample_Number colorBarMinimum double 0.0
attribute Sample_Number description String Indicator to distinguish specific individuals
attribute Sample_Number long_name String Sample Number
attribute Sample_Number nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Sample_Number units String unitless
variable Mg   double  
attribute Mg _FillValue double NaN
attribute Mg actual_range double 0.0, 1.913999631E10
attribute Mg bcodmo_name String trace_metal_conc
attribute Mg description String Magnesium concentration (umol Mg/mol Ca)
attribute Mg long_name String MG
attribute Mg nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Mg units String dimensionless
variable Mn   double  
attribute Mn _FillValue double NaN
attribute Mn actual_range double 0.0, 6.497053151E7
attribute Mn bcodmo_name String trace_metal_conc
attribute Mn description String Manganese concentration (umol Mn/mol Ca)
attribute Mn long_name String MN
attribute Mn nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Mn units String dimensionless
variable Co   double  
attribute Co _FillValue double NaN
attribute Co actual_range double 0.0, 242523.26
attribute Co bcodmo_name String trace_metal_conc
attribute Co description String Cobalt concentration (umol Co/mol Ca)
attribute Co long_name String Co
attribute Co nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Co units String dimensionless
variable Sr   double  
attribute Sr _FillValue double NaN
attribute Sr actual_range double 0.0, 2.68862335914E11
attribute Sr bcodmo_name String trace_metal_conc
attribute Sr description String Strontium concentration (umol Sr/mol Ca)
attribute Sr long_name String SR
attribute Sr nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Sr units String dimensionless
variable Ba   double  
attribute Ba _FillValue double NaN
attribute Ba actual_range double 0.0, 5.912782338E7
attribute Ba bcodmo_name String trace_metal_conc
attribute Ba description String Barium concentration (umol Ba/mol Ca)
attribute Ba long_name String Ba
attribute Ba nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Ba units String dimensionless
variable La   double  
attribute La _FillValue double NaN
attribute La actual_range double 0.0, 1.885516664E7
attribute La bcodmo_name String trace_metal_conc
attribute La description String Lanthanum concentration (umol La/mol Ca)
attribute La long_name String La
attribute La nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute La units String dimensionless
variable Pb   double  
attribute Pb _FillValue double NaN
attribute Pb actual_range double 0.0, 2113952.36
attribute Pb bcodmo_name String trace_metal_conc
attribute Pb description String Lead concentration (umol Pb/mol Ca)
attribute Pb long_name String PB
attribute Pb nerc_identifier String https://vocab.nerc.ac.uk/collection/P03/current/C035/ (external link)
attribute Pb units String dimensionless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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