Accessing BCO-DMO data
log in    
Brought to you by BCO-DMO    

ERDDAP > info > bcodmo_dataset_752974

Title Sum-
Institution Dataset ID
     data   graph     files  public Body characteristic measurements of sand dollar larvae (Dendraster excentricus) reared in
different pCO2 conditions, July 2017
   ?     I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_752974

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String We collected adult sand dollars (D. excentricus) from Semiahmoo Bay, WA, in
July 2017 and maintained them in 14\u00b0C continuous flowing seawater at the
Shannon Point Marine Center. We induced twelve individuals to spawn by
injecting 1-mL of 0.5-M KCl into the coelom following methods outlined by
Strathmann (1987).\u00a0 We then collected and mixed concentrated gametes of
four males and four females for fertilization. We added five drops of sperm to
500-mL of filtered seawater and 5-mL of eggs. We placed the fertilized eggs in
12\u00b0C incubator and bubbled them with ambient pCO2 condition for 12-hrs
before dividing the embryos into pCO2 treatment conditions before

We reared D. excentricus larvae (2 individuals mL-1) at 12\u00b0C in eight 3-L
jars that were individually bubbled with CO2 to achieve four replicates of
ambient (400ppm) and acidic (1500ppm) pCO2 conditions. Daily we gave each
larval jar a water change from pre-equilibrated ambient and acidic water and
fed the larvae D. tertiolecta (6,000 cells ml-1).

We measured morphological characteristics of larvae from each rearing jar when
larvae were in the 4- and 6-arm stages following diagrams in Chan et al.
(2011) and Reitzel et al. (2004). Larvae were relaxed in 7% magnesium
chloride, fixed in 3% formalin, imaged using a stereomicroscope equipped with
a camera (Leica MC170 HD and Leica Application Suite, Leica, Wetzlar,
Germany), and measured with ImageJ software. 4-arm larvae were fixed on
7/24/17 and 6-arm larvae were fixed on 7/28/18. The following morphological
characteristics were measured: length of postoral arms (PO), anterolateral
arms (AL), length of body midline, body width, and longitudinal length of

This dataset includes unprocessed data.
attribute NC_GLOBAL awards_0_award_nid String 684166
attribute NC_GLOBAL awards_0_award_number String OCE-1538626
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538626 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Dendraster Characters_OA Expt, 2017
S. Arellano, B. Olson, S. Yang (WWU)
version: 2019-01-14
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2019-01-16T20:14:50Z
attribute NC_GLOBAL date_modified String 2019-09-25T19:39:12Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.752974.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/752974 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL keywords String AL_1, AL_2, bco, bco-dmo, biological, body, Body_ML, chemical, condition, data, dataset, date, dmo, erddap, jar, jar_replicate, management, oceanography, office, PD_1, PD_2, PO_1, PO_2, preliminary, rearing, rearing_condition, replicate, stomach, time
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/752974/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/752974 (external link)
attribute NC_GLOBAL param_mapping String {'752974': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/752974/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Western Washington University
attribute NC_GLOBAL people_0_affiliation_acronym String WWU
attribute NC_GLOBAL people_0_person_name String Shawn M Arellano
attribute NC_GLOBAL people_0_person_nid String 684169
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Western Washington University
attribute NC_GLOBAL people_1_affiliation_acronym String WWU
attribute NC_GLOBAL people_1_person_name String Dr Brady M. Olson
attribute NC_GLOBAL people_1_person_nid String 51528
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Western Washington University
attribute NC_GLOBAL people_2_affiliation_acronym String WWU
attribute NC_GLOBAL people_2_person_name String Dr Sylvia Yang
attribute NC_GLOBAL people_2_person_nid String 684172
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Nancy Copley
attribute NC_GLOBAL people_3_person_nid String 50396
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String Climate stressors on larvae
attribute NC_GLOBAL projects_0_acronym String Climate stressors on larvae
attribute NC_GLOBAL projects_0_description String In the face of climate change, future distribution of animals will depend not only on whether they adjust to new conditions in their current habitat, but also on whether a species can spread to suitable locations in a changing habitat landscape. In the ocean, where most species have tiny drifting larval stages, dispersal between habitats is impacted by more than just ocean currents alone; the swimming behavior of larvae, the flow environment the larvae encounter, and the length of time the larvae spend in the water column all interact to impact the distance and direction of larval dispersal. The effects of climate change, especially ocean acidification, are already evident in shellfish species along the Pacific coast, where hatchery managers have noticed shellfish cultures with 'lazy larvae syndrome.' Under conditions of increased acidification, these 'lazy larvae' simply stop swimming; yet, larval swimming behavior is rarely incorporated into studies of ocean acidification. Furthermore, how ocean warming interacts with the effects of acidification on larvae and their swimming behaviors remains unexplored; indeed, warming could reverse 'lazy larvae syndrome.' This project uses a combination of manipulative laboratory experiments, computer modeling, and a real case study to examine whether the impacts of ocean warming and acidification on individual larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea. The project will tightly couple research with undergraduate education at Western Washington University, a primarily undergraduate university, by employing student researchers, incorporating materials into undergraduate courses, and pairing marine science student interns with art student interns to develop art projects aimed at communicating the effects of climate change to public audiences
As studies of the effects of climate stress in the marine environment progress, impacts on individual-level performance must be placed in a larger ecological context. While future climate-induced circulation changes certainly will affect larval dispersal, the effects of climate-change stressors on individual larval traits alone may have equally important impacts, significantly altering larval transport and, ultimately, species distribution. This study will experimentally examine the relationship between combined climate stressors (warming and acidification) on planktonic larval duration, morphology, and swimming behavior; create models to generate testable hypotheses about the effects of these factors on larval dispersal that can be applied across systems; and, finally, use a bio-physically coupled larval transport model to examine whether climate-impacted larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea.
attribute NC_GLOBAL projects_0_end_date String 2018-08
attribute NC_GLOBAL projects_0_geolocation String Coastal Pacific, USA
attribute NC_GLOBAL projects_0_name String RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal
attribute NC_GLOBAL projects_0_project_nid String 684167
attribute NC_GLOBAL projects_0_start_date String 2015-09
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String This dataset includes body characteristic measurements of sand dollar larvae (Dendraster excentricus) reared in different pCO2 conditions as part of a laboratory experiment to investigate the behavioral effects of ocean acidification on this species in July 2017.
attribute NC_GLOBAL title String Body characteristic measurements of sand dollar larvae (Dendraster excentricus) reared in different pCO2 conditions, July 2017
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable Date   String  
attribute Date bcodmo_name String date_local
attribute Date description String Date larvae were fixed formatted as yyyy-mm-dd
attribute Date long_name String Date
attribute Date source_name String Date
attribute Date time_precision String 1970-01-01
attribute Date units String unitless
variable rearing_condition   String  
attribute rearing_condition bcodmo_name String treatment
attribute rearing_condition description String The pH condition of the water larvae were reared in; "acidic" water was bubbled to be 1500ppm and "ambient" water was bubbled to be 400ppm
attribute rearing_condition long_name String Rearing Condition
attribute rearing_condition units String unitless
variable jar_replicate   String  
attribute jar_replicate bcodmo_name String replicate
attribute jar_replicate description String Jar replicate larvae were sampled from. There were four replicate jars per rearing condition.
attribute jar_replicate long_name String Jar Replicate
attribute jar_replicate units String unitless
variable PO_1   float  
attribute PO_1 _FillValue float NaN
attribute PO_1 actual_range float 222.65, 544.02
attribute PO_1 bcodmo_name String length
attribute PO_1 description String Postoral arm length; one of two
attribute PO_1 long_name String PO 1
attribute PO_1 units String micrometers (um)
variable PO_2   float  
attribute PO_2 _FillValue float NaN
attribute PO_2 actual_range float 224.54, 519.02
attribute PO_2 bcodmo_name String length
attribute PO_2 description String Postoral arm length; one of two
attribute PO_2 long_name String PO 2
attribute PO_2 units String micrometers (um)
variable AL_1   float  
attribute AL_1 _FillValue float NaN
attribute AL_1 actual_range float 44.53, 565.19
attribute AL_1 bcodmo_name String length
attribute AL_1 description String Anterolateral arm length; one of two
attribute AL_1 long_name String AL 1
attribute AL_1 units String micrometers (um)
variable AL_2   float  
attribute AL_2 _FillValue float NaN
attribute AL_2 actual_range float 243.2, 429.39
attribute AL_2 bcodmo_name String length
attribute AL_2 description String Anterolateral arm length; one of two
attribute AL_2 long_name String AL 2
attribute AL_2 units String micrometers (um)
variable Body_ML   float  
attribute Body_ML _FillValue float NaN
attribute Body_ML actual_range float 208.28, 447.95
attribute Body_ML bcodmo_name String length
attribute Body_ML description String Length of body midline
attribute Body_ML long_name String Body ML
attribute Body_ML units String micrometers (um)
variable BW   float  
attribute BW _FillValue float NaN
attribute BW actual_range float 94.39, 1992.67
attribute BW bcodmo_name String length
attribute BW description String Length of body width
attribute BW long_name String BW
attribute BW units String micrometers (um)
variable PD_1   float  
attribute PD_1 _FillValue float NaN
attribute PD_1 actual_range float 141.68, 469.57
attribute PD_1 bcodmo_name String length
attribute PD_1 description String Posterodorsal arm length; one of two
attribute PD_1 long_name String PD 1
attribute PD_1 units String micrometers (um)
variable PD_2   float  
attribute PD_2 _FillValue float NaN
attribute PD_2 actual_range float 183.24, 427.82
attribute PD_2 bcodmo_name String length
attribute PD_2 description String Posterodorsal arm length; one of two
attribute PD_2 long_name String PD 2
attribute PD_2 units String micrometers (um)
variable Stomach   float  
attribute Stomach _FillValue float NaN
attribute Stomach actual_range float 53.67, 162.01
attribute Stomach bcodmo_name String length
attribute Stomach description String Longitudinal length of stomach
attribute Stomach long_name String Stomach
attribute Stomach units String micrometers (um)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

ERDDAP, Version 2.02
Disclaimers | Privacy Policy | Contact