ERDDAP > info > bcodmo_dataset_752974

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	Body characteristic measurements of sand dollar larvae (Dendraster excentricus) reared in different pCO2 conditions, July 2017		I   M	background			BCO-DMO	bcodmo_dataset_752974

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	We collected adult sand dollars (D. excentricus) from Semiahmoo Bay, WA, in July 2017 and maintained them in 14\u00b0C continuous flowing seawater at the Shannon Point Marine Center. We induced twelve individuals to spawn by injecting 1-mL of 0.5-M KCl into the coelom following methods outlined by Strathmann (1987).\u00a0 We then collected and mixed concentrated gametes of four males and four females for fertilization. We added five drops of sperm to 500-mL of filtered seawater and 5-mL of eggs. We placed the fertilized eggs in 12\u00b0C incubator and bubbled them with ambient pCO2 condition for 12-hrs before dividing the embryos into pCO2 treatment conditions before gastrulation. We reared D. excentricus larvae (2 individuals mL-1) at 12\u00b0C in eight 3-L jars that were individually bubbled with CO2 to achieve four replicates of ambient (400ppm) and acidic (1500ppm) pCO2 conditions. Daily we gave each larval jar a water change from pre-equilibrated ambient and acidic water and fed the larvae D. tertiolecta (6,000 cells ml-1). We measured morphological characteristics of larvae from each rearing jar when larvae were in the 4- and 6-arm stages following diagrams in Chan et al. (2011) and Reitzel et al. (2004). Larvae were relaxed in 7% magnesium chloride, fixed in 3% formalin, imaged using a stereomicroscope equipped with a camera (Leica MC170 HD and Leica Application Suite, Leica, Wetzlar, Germany), and measured with ImageJ software. 4-arm larvae were fixed on 7/24/17 and 6-arm larvae were fixed on 7/28/18. The following morphological characteristics were measured: length of postoral arms (PO), anterolateral arms (AL), length of body midline, body width, and longitudinal length of stomach. This dataset includes unprocessed data.
attribute	NC_GLOBAL	awards_0_award_nid	String	684166
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1538626
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538626
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Michael E. Sieracki
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50446
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Dendraster Characters_OA Expt, 2017 S. Arellano, B. Olson, S. Yang (WWU) version: 2019-01-14
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2019-01-16T20:14:50Z
attribute	NC_GLOBAL	date_modified	String	2019-09-25T19:39:12Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.752974.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/752974
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	keywords	String	AL_1, AL_2, bco, bco-dmo, biological, body, Body_ML, chemical, condition, data, dataset, date, dmo, erddap, jar, jar_replicate, management, oceanography, office, PD_1, PD_2, PO_1, PO_2, preliminary, rearing, rearing_condition, replicate, stomach, time
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/752974/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/752974
attribute	NC_GLOBAL	param_mapping	String	{'752974': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/752974/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Western Washington University
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	WWU
attribute	NC_GLOBAL	people_0_person_name	String	Shawn M Arellano
attribute	NC_GLOBAL	people_0_person_nid	String	684169
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Western Washington University
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WWU
attribute	NC_GLOBAL	people_1_person_name	String	Dr Brady M. Olson
attribute	NC_GLOBAL	people_1_person_nid	String	51528
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Western Washington University
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	WWU
attribute	NC_GLOBAL	people_2_person_name	String	Dr Sylvia Yang
attribute	NC_GLOBAL	people_2_person_nid	String	684172
attribute	NC_GLOBAL	people_2_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_3_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_3_person_nid	String	50396
attribute	NC_GLOBAL	people_3_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	project	String	Climate stressors on larvae
attribute	NC_GLOBAL	projects_0_acronym	String	Climate stressors on larvae
attribute	NC_GLOBAL	projects_0_description	String	In the face of climate change, future distribution of animals will depend not only on whether they adjust to new conditions in their current habitat, but also on whether a species can spread to suitable locations in a changing habitat landscape. In the ocean, where most species have tiny drifting larval stages, dispersal between habitats is impacted by more than just ocean currents alone; the swimming behavior of larvae, the flow environment the larvae encounter, and the length of time the larvae spend in the water column all interact to impact the distance and direction of larval dispersal. The effects of climate change, especially ocean acidification, are already evident in shellfish species along the Pacific coast, where hatchery managers have noticed shellfish cultures with 'lazy larvae syndrome.' Under conditions of increased acidification, these 'lazy larvae' simply stop swimming; yet, larval swimming behavior is rarely incorporated into studies of ocean acidification. Furthermore, how ocean warming interacts with the effects of acidification on larvae and their swimming behaviors remains unexplored; indeed, warming could reverse 'lazy larvae syndrome.' This project uses a combination of manipulative laboratory experiments, computer modeling, and a real case study to examine whether the impacts of ocean warming and acidification on individual larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea. The project will tightly couple research with undergraduate education at Western Washington University, a primarily undergraduate university, by employing student researchers, incorporating materials into undergraduate courses, and pairing marine science student interns with art student interns to develop art projects aimed at communicating the effects of climate change to public audiences As studies of the effects of climate stress in the marine environment progress, impacts on individual-level performance must be placed in a larger ecological context. While future climate-induced circulation changes certainly will affect larval dispersal, the effects of climate-change stressors on individual larval traits alone may have equally important impacts, significantly altering larval transport and, ultimately, species distribution. This study will experimentally examine the relationship between combined climate stressors (warming and acidification) on planktonic larval duration, morphology, and swimming behavior; create models to generate testable hypotheses about the effects of these factors on larval dispersal that can be applied across systems; and, finally, use a bio-physically coupled larval transport model to examine whether climate-impacted larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea.
attribute	NC_GLOBAL	projects_0_end_date	String	2018-08
attribute	NC_GLOBAL	projects_0_geolocation	String	Coastal Pacific, USA
attribute	NC_GLOBAL	projects_0_name	String	RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal
attribute	NC_GLOBAL	projects_0_project_nid	String	684167
attribute	NC_GLOBAL	projects_0_start_date	String	2015-09
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	This dataset includes body characteristic measurements of sand dollar larvae (Dendraster excentricus) reared in different pCO2 conditions as part of a laboratory experiment to investigate the behavioral effects of ocean acidification on this species in July 2017.
attribute	NC_GLOBAL	title	String	Body characteristic measurements of sand dollar larvae (Dendraster excentricus) reared in different pCO2 conditions, July 2017
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Date		String
attribute	Date	bcodmo_name	String	date_local
attribute	Date	description	String	Date larvae were fixed formatted as yyyy-mm-dd
attribute	Date	long_name	String	Date
attribute	Date	source_name	String	Date
attribute	Date	time_precision	String	1970-01-01
attribute	Date	units	String	unitless
variable	rearing_condition		String
attribute	rearing_condition	bcodmo_name	String	treatment
attribute	rearing_condition	description	String	The pH condition of the water larvae were reared in; "acidic" water was bubbled to be 1500ppm and "ambient" water was bubbled to be 400ppm
attribute	rearing_condition	long_name	String	Rearing Condition
attribute	rearing_condition	units	String	unitless
variable	jar_replicate		String
attribute	jar_replicate	bcodmo_name	String	replicate
attribute	jar_replicate	description	String	Jar replicate larvae were sampled from. There were four replicate jars per rearing condition.
attribute	jar_replicate	long_name	String	Jar Replicate
attribute	jar_replicate	units	String	unitless
variable	PO_1		float
attribute	PO_1	_FillValue	float	NaN
attribute	PO_1	actual_range	float	222.65, 544.02
attribute	PO_1	bcodmo_name	String	length
attribute	PO_1	description	String	Postoral arm length; one of two
attribute	PO_1	long_name	String	PO 1
attribute	PO_1	units	String	micrometers (um)
variable	PO_2		float
attribute	PO_2	_FillValue	float	NaN
attribute	PO_2	actual_range	float	224.54, 519.02
attribute	PO_2	bcodmo_name	String	length
attribute	PO_2	description	String	Postoral arm length; one of two
attribute	PO_2	long_name	String	PO 2
attribute	PO_2	units	String	micrometers (um)
variable	AL_1		float
attribute	AL_1	_FillValue	float	NaN
attribute	AL_1	actual_range	float	44.53, 565.19
attribute	AL_1	bcodmo_name	String	length
attribute	AL_1	description	String	Anterolateral arm length; one of two
attribute	AL_1	long_name	String	AL 1
attribute	AL_1	units	String	micrometers (um)
variable	AL_2		float
attribute	AL_2	_FillValue	float	NaN
attribute	AL_2	actual_range	float	243.2, 429.39
attribute	AL_2	bcodmo_name	String	length
attribute	AL_2	description	String	Anterolateral arm length; one of two
attribute	AL_2	long_name	String	AL 2
attribute	AL_2	units	String	micrometers (um)
variable	Body_ML		float
attribute	Body_ML	_FillValue	float	NaN
attribute	Body_ML	actual_range	float	208.28, 447.95
attribute	Body_ML	bcodmo_name	String	length
attribute	Body_ML	description	String	Length of body midline
attribute	Body_ML	long_name	String	Body ML
attribute	Body_ML	units	String	micrometers (um)
variable	BW		float
attribute	BW	_FillValue	float	NaN
attribute	BW	actual_range	float	94.39, 1992.67
attribute	BW	bcodmo_name	String	length
attribute	BW	description	String	Length of body width
attribute	BW	long_name	String	BW
attribute	BW	units	String	micrometers (um)
variable	PD_1		float
attribute	PD_1	_FillValue	float	NaN
attribute	PD_1	actual_range	float	141.68, 469.57
attribute	PD_1	bcodmo_name	String	length
attribute	PD_1	description	String	Posterodorsal arm length; one of two
attribute	PD_1	long_name	String	PD 1
attribute	PD_1	units	String	micrometers (um)
variable	PD_2		float
attribute	PD_2	_FillValue	float	NaN
attribute	PD_2	actual_range	float	183.24, 427.82
attribute	PD_2	bcodmo_name	String	length
attribute	PD_2	description	String	Posterodorsal arm length; one of two
attribute	PD_2	long_name	String	PD 2
attribute	PD_2	units	String	micrometers (um)
variable	Stomach		float
attribute	Stomach	_FillValue	float	NaN
attribute	Stomach	actual_range	float	53.67, 162.01
attribute	Stomach	bcodmo_name	String	length
attribute	Stomach	description	String	Longitudinal length of stomach
attribute	Stomach	long_name	String	Stomach
attribute	Stomach	units	String	micrometers (um)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.