ERDDAP > info > bcodmo_dataset_753036

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	Lipid analysis data from experiment on grazing and physiological effects of ocean acidification on sand dollar larvae (Dendraster excentricus), July 2017		I   M	background			BCO-DMO	bcodmo_dataset_753036

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Spawning and fertilization We collected adult sand dollars (D. excentricus) from Semiahmoo Bay, WA, on July 7, 2017, and maintained them in 14\u00b0C continuous flowing seawater at the Shannon Point Marine Center. On July 12, 2017, we induced twelve individuals to spawn by injecting 1-mL of 0.5-M KCl into the coelom following methods outlined by Strathmann (1987).\u00a0 We then collected and mixed concentrated gametes of four males and four females for fertilization. We added five drops of sperm to 500-mL of filtered seawater and 5-mL of eggs. We placed the fertilized eggs in 12\u00b0C incubator and bubbled them with ambient pCO2 condition for 12-hrs before dividing the embryos into pCO2 treatment conditions before gastrulation. We then counted and transferred the larvae into jars with 1.5 L of nanopore filtered seawater at densities of 1-2 individuals mL-1. Grazing experiment To assess the interactive effects of high temperature and pCO2 on Dunaliella excentricus feeding behavior, our experimental design had six treatments with four experimental jars (replicates) in each. The treatments combined three levels of CO2: 400 ppmv (ambient atmospheric level), 800 ppmv (moderate atmoshpheric level) and 1,500 ppmv (high atmospheric level), and two temperatures: 12\u00b0C (ambient temperature) and 17\u00b0C (high temperature). We fed D. tertiolecta at approximately 6,000 cells ml-1 to six- arm stage larvae to evaluate feeding rates at each treatment condition. For each replicate, a corresponding 150-mL control bottle containing only D. tertiolecta was also prepared. Feeding rate was estimated as ingestion rate by measuring the algal concentration (cells ml-1) at the beginning (T0) and after 24 hours (Tf) in control bottles and experimental jars using a Sedgewick Rafter Chamber (Stumpp et al., 2011). Ingestion rate (cells ind-1 hr-1) was calculated as I = (Clearance rate) x (time-average algae concentration).\u00a0 Lipid storage analysis At the end of the long-term experiment, larval lipid index was assessed using a procedure adapted from Talmage et. al (2010).\u00a0 First, we randomly selected D. excentricus larvae from each treatment and stained them with Nile Red dissolved in acetone. Nile Red stains intracellular lipid droplets bright yellow. Larvae were exposed to the stain for \u223c1.5 h, after which they were photographed under an epi-fluorescent microscope (Leica 80i) within 4 hours of being stained\u00a0 (Ko et al., 2014). The lipid areas of approximately 5\u221215 larvae per sample were measured using the ImageJ software. The lipid index was calculated by dividing the area of the larva stomach containing the fluorescing lipids by the total stomach area (Talmage & Gobler, 2010).\u00a0
attribute	NC_GLOBAL	awards_0_award_nid	String	684166
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1538626
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538626
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Michael E. Sieracki
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50446
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Dendraster Lipid Analysis - OA Expt 2017 S. Arellano, B. Olson, S. Yang (WWU) version: 2019-01-14
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2019-01-16T21:40:53Z
attribute	NC_GLOBAL	date_modified	String	2019-09-25T20:02:15Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.753036.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/753036
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used to photograph larval intracellular lipid droplets.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	753043
attribute	NC_GLOBAL	instruments_0_description	String	Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB06/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Microscope-Fluorescence
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	695
attribute	NC_GLOBAL	instruments_0_supplied_name	String	epi-fluorescent microscope (Leica 80i)
attribute	NC_GLOBAL	keywords	String	area, bco, bco-dmo, biological, chemical, data, dataset, dmo, erddap, index, jar, jar_replicate, lipid, lipid_area, lipid_index, lipid_presence, management, oceanography, office, pH_treatment, preliminary, presence, replicate, stomach, stomach_area, temp_treatment, temperature, treatment
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/753036/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/753036
attribute	NC_GLOBAL	param_mapping	String	{'753036': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/753036/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Western Washington University
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	WWU
attribute	NC_GLOBAL	people_0_person_name	String	Shawn M Arellano
attribute	NC_GLOBAL	people_0_person_nid	String	684169
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Western Washington University
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WWU
attribute	NC_GLOBAL	people_1_person_name	String	Dr Brady M. Olson
attribute	NC_GLOBAL	people_1_person_nid	String	51528
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Western Washington University
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	WWU
attribute	NC_GLOBAL	people_2_person_name	String	Dr Sylvia Yang
attribute	NC_GLOBAL	people_2_person_nid	String	684172
attribute	NC_GLOBAL	people_2_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_3_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_3_person_nid	String	50396
attribute	NC_GLOBAL	people_3_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	project	String	Climate stressors on larvae
attribute	NC_GLOBAL	projects_0_acronym	String	Climate stressors on larvae
attribute	NC_GLOBAL	projects_0_description	String	In the face of climate change, future distribution of animals will depend not only on whether they adjust to new conditions in their current habitat, but also on whether a species can spread to suitable locations in a changing habitat landscape. In the ocean, where most species have tiny drifting larval stages, dispersal between habitats is impacted by more than just ocean currents alone; the swimming behavior of larvae, the flow environment the larvae encounter, and the length of time the larvae spend in the water column all interact to impact the distance and direction of larval dispersal. The effects of climate change, especially ocean acidification, are already evident in shellfish species along the Pacific coast, where hatchery managers have noticed shellfish cultures with 'lazy larvae syndrome.' Under conditions of increased acidification, these 'lazy larvae' simply stop swimming; yet, larval swimming behavior is rarely incorporated into studies of ocean acidification. Furthermore, how ocean warming interacts with the effects of acidification on larvae and their swimming behaviors remains unexplored; indeed, warming could reverse 'lazy larvae syndrome.' This project uses a combination of manipulative laboratory experiments, computer modeling, and a real case study to examine whether the impacts of ocean warming and acidification on individual larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea. The project will tightly couple research with undergraduate education at Western Washington University, a primarily undergraduate university, by employing student researchers, incorporating materials into undergraduate courses, and pairing marine science student interns with art student interns to develop art projects aimed at communicating the effects of climate change to public audiences As studies of the effects of climate stress in the marine environment progress, impacts on individual-level performance must be placed in a larger ecological context. While future climate-induced circulation changes certainly will affect larval dispersal, the effects of climate-change stressors on individual larval traits alone may have equally important impacts, significantly altering larval transport and, ultimately, species distribution. This study will experimentally examine the relationship between combined climate stressors (warming and acidification) on planktonic larval duration, morphology, and swimming behavior; create models to generate testable hypotheses about the effects of these factors on larval dispersal that can be applied across systems; and, finally, use a bio-physically coupled larval transport model to examine whether climate-impacted larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea.
attribute	NC_GLOBAL	projects_0_end_date	String	2018-08
attribute	NC_GLOBAL	projects_0_geolocation	String	Coastal Pacific, USA
attribute	NC_GLOBAL	projects_0_name	String	RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal
attribute	NC_GLOBAL	projects_0_project_nid	String	684167
attribute	NC_GLOBAL	projects_0_start_date	String	2015-09
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Lipid analysis data collected from a laboratory experiment to investigate the grazing and physiological effects of ocean acidification on sand dollar larvae (Dendraster excentricus), July 2017.
attribute	NC_GLOBAL	title	String	Lipid analysis data from experiment on grazing and physiological effects of ocean acidification on sand dollar larvae (Dendraster excentricus), July 2017
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	temp_treatment		byte
attribute	temp_treatment	_FillValue	byte	127
attribute	temp_treatment	actual_range	byte	12, 17
attribute	temp_treatment	bcodmo_name	String	treatment
attribute	temp_treatment	description	String	The temperature condition of the water larvae were reared within
attribute	temp_treatment	long_name	String	Temp Treatment
attribute	temp_treatment	units	String	degrees Celsius
variable	pH_treatment		String
attribute	pH_treatment	bcodmo_name	String	treatment
attribute	pH_treatment	description	String	The pH condition of the water larvae were reared within: L = treatment of 400ppm; M = treatment of 800pm; H = treatment of 1500ppm
attribute	pH_treatment	long_name	String	P H Treatment
attribute	pH_treatment	units	String	unitless
variable	jar_replicate		byte
attribute	jar_replicate	_FillValue	byte	127
attribute	jar_replicate	actual_range	byte	1, 15
attribute	jar_replicate	bcodmo_name	String	replicate
attribute	jar_replicate	description	String	Replicate of the pH and temperature treatment combination. Four jars were maintained at each treatment.
attribute	jar_replicate	long_name	String	Jar Replicate
attribute	jar_replicate	units	String	unitless
variable	lipid_presence		String
attribute	lipid_presence	bcodmo_name	String	flag
attribute	lipid_presence	description	String	Is lipid present within measured larva? Yes or No
attribute	lipid_presence	long_name	String	Lipid Presence
attribute	lipid_presence	units	String	unitless
variable	lipid_area		double
attribute	lipid_area	_FillValue	double	NaN
attribute	lipid_area	actual_range	double	0.0, 13250.238
attribute	lipid_area	bcodmo_name	String	surface_area
attribute	lipid_area	description	String	Area of larva's lipid content
attribute	lipid_area	long_name	String	Lipid Area
attribute	lipid_area	units	String	pixels per micron
variable	stomach_area		double
attribute	stomach_area	_FillValue	double	NaN
attribute	stomach_area	actual_range	double	4692.515, 19999.86
attribute	stomach_area	bcodmo_name	String	surface_area
attribute	stomach_area	description	String	Area of larva's stomach
attribute	stomach_area	long_name	String	Stomach Area
attribute	stomach_area	units	String	pixels per micron
variable	lipid_index		float
attribute	lipid_index	_FillValue	float	NaN
attribute	lipid_index	actual_range	float	0.0, 0.924
attribute	lipid_index	bcodmo_name	String	unknown
attribute	lipid_index	description	String	Area of larva's lipid content divided by area of larva's stomach
attribute	lipid_index	long_name	String	Lipid Index
attribute	lipid_index	units	String	unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.