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Title Sum-
Institution Dataset ID
     data   graph     files  public Oyster larvae vertical distribution data collected from laboratory water column experiments
on the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida),
July 2017
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Collection & Larval Rearing

\u00a0We collected adult Olympia oysters (Ostrea lurida) from Fidalgo Bay in
June 2017 and maintained them in a sea table with continuous flowing seawater
heated to 19-20\u00b0C at the Shannon Point Marine Center. We fed adult
oysters were fed concentrated algae once a day (Shellfish Diet, Reed
Mariculture) and utilized banjo-style filters (60-m) attached to the outflow
pipes of the sea table to catch released O. lurida larvae. We then collected
and reared larvae at 12\u00b0C in 3-L jars (2 individuals mL-1). Each jar of
larvae received a 50% water change with 0.35-m filtered sea water and were fed
Isochrysis galbana algae (50,000 cells mL-1) daily.

\u00a0Experimental Design

\u00a0To measure the effect of pH conditions on the vertical distribution of
larvae we established three experimental pycnocline treatments within clear
plexiglass water columns (2.5cm x 2.5cm x 30cm): (1) ambient water (400ppm) in
the top layer and acidic water in the bottom layer (1500ppm), (2) ambient
water (400ppm) in both top and bottom layers, and (3) acidic water (1500ppm)
in the top layer and ambient water (400ppm) in the bottom layer. Each water
layer was 60-mL of water and filled the column 10-cm high, so when each
experimental treatment was established it filled the column to 20-cm. We
established the experimental treatments by increasing the density of seawater
in the bottom layer by 0.003-0.005 g ml-1 using PercollTM GE Healthcare
(Podolsky & Emlet 1993). Experimental treatment water was kept at 12\u00b0C
and pre-equilibrated to the desired pCO2 level and density. We also included
blue food coloring (1 drop per 100-mL) to the dense bottom layer to more
easily visualize the density layers while establishing experimental
treatments. We set-up four replicate columns for each experimental treatment
making twelve columns total per experiment.

\u00a0On the day of each experiment, we incubated the experimental treatment
columns in clear plexiglass water baths connected to a Fisher Scientific
Isotemp recirculating water bath to maintain treatment temperature at
12\u00b0C throughout the experiment. We carefully injected 150 larvae by
syringe into the bottom 2-cm of each column with no more than 2-mL of their
culture water. Olympia oyster larvae are highly phototactic (personal
observations), so larvae were kept in the dark and we video recorded their
vertical positions under infrared light two times: the first time at 10
minutes of acclimation in the columns and the second time at 30 minutes of
acclimation in the columns. To record, we used an infrared uEye camera
equipped with Edmund Optics VIS-NIR Lens mounted on a motorized stand. We
later counted by eye the number of larvae per centimeter area of each column
from the videos.
attribute NC_GLOBAL awards_0_award_nid String 684166
attribute NC_GLOBAL awards_0_award_number String OCE-1538626
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538626 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Oyster larvae vertical distribution
lab expts, July 2017
S. Arellano, B. Olson, S. Yang (WWU)
version: 2019-01-14
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2019-01-17T13:32:22Z
attribute NC_GLOBAL date_modified String 2019-09-25T19:43:40Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.753058.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/753058 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String in-situ incubator
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to maintain treatment temperature during experiment
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 753069
attribute NC_GLOBAL instruments_0_description String A device on shipboard or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/82/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String In-situ incubator
attribute NC_GLOBAL instruments_0_instrument_nid String 494
attribute NC_GLOBAL instruments_0_supplied_name String Fisher Scientific Isotemp Circulating Water Bath
attribute NC_GLOBAL instruments_1_acronym String camera
attribute NC_GLOBAL instruments_1_dataset_instrument_description String uEye camera equipped with Edmund Optics VIS-NIR Lens mounted on a motorized stand
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 753070
attribute NC_GLOBAL instruments_1_description String All types of photographic equipment including stills, video, film and digital systems.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/311/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Camera
attribute NC_GLOBAL instruments_1_instrument_nid String 520
attribute NC_GLOBAL instruments_1_supplied_name String uEye video camera
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, chemical, column, column_name, count, count_id, data, dataset, date, dmo, erddap, height, height_cm, larvae, larvae_count, management, middepth, middepth_cm, name, oceanography, office, preliminary, proportion, proportion_larvae, time, trial
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/753058/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/753058 (external link)
attribute NC_GLOBAL param_mapping String {'753058': {'middepth_cm': 'master - depth'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/753058/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Western Washington University
attribute NC_GLOBAL people_0_affiliation_acronym String WWU
attribute NC_GLOBAL people_0_person_name String Shawn M Arellano
attribute NC_GLOBAL people_0_person_nid String 684169
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Western Washington University
attribute NC_GLOBAL people_1_affiliation_acronym String WWU
attribute NC_GLOBAL people_1_person_name String Dr Brady M. Olson
attribute NC_GLOBAL people_1_person_nid String 51528
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Western Washington University
attribute NC_GLOBAL people_2_affiliation_acronym String WWU
attribute NC_GLOBAL people_2_person_name String Dr Sylvia Yang
attribute NC_GLOBAL people_2_person_nid String 684172
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Nancy Copley
attribute NC_GLOBAL people_3_person_nid String 50396
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String Climate stressors on larvae
attribute NC_GLOBAL projects_0_acronym String Climate stressors on larvae
attribute NC_GLOBAL projects_0_description String In the face of climate change, future distribution of animals will depend not only on whether they adjust to new conditions in their current habitat, but also on whether a species can spread to suitable locations in a changing habitat landscape. In the ocean, where most species have tiny drifting larval stages, dispersal between habitats is impacted by more than just ocean currents alone; the swimming behavior of larvae, the flow environment the larvae encounter, and the length of time the larvae spend in the water column all interact to impact the distance and direction of larval dispersal. The effects of climate change, especially ocean acidification, are already evident in shellfish species along the Pacific coast, where hatchery managers have noticed shellfish cultures with 'lazy larvae syndrome.' Under conditions of increased acidification, these 'lazy larvae' simply stop swimming; yet, larval swimming behavior is rarely incorporated into studies of ocean acidification. Furthermore, how ocean warming interacts with the effects of acidification on larvae and their swimming behaviors remains unexplored; indeed, warming could reverse 'lazy larvae syndrome.' This project uses a combination of manipulative laboratory experiments, computer modeling, and a real case study to examine whether the impacts of ocean warming and acidification on individual larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea. The project will tightly couple research with undergraduate education at Western Washington University, a primarily undergraduate university, by employing student researchers, incorporating materials into undergraduate courses, and pairing marine science student interns with art student interns to develop art projects aimed at communicating the effects of climate change to public audiences
As studies of the effects of climate stress in the marine environment progress, impacts on individual-level performance must be placed in a larger ecological context. While future climate-induced circulation changes certainly will affect larval dispersal, the effects of climate-change stressors on individual larval traits alone may have equally important impacts, significantly altering larval transport and, ultimately, species distribution. This study will experimentally examine the relationship between combined climate stressors (warming and acidification) on planktonic larval duration, morphology, and swimming behavior; create models to generate testable hypotheses about the effects of these factors on larval dispersal that can be applied across systems; and, finally, use a bio-physically coupled larval transport model to examine whether climate-impacted larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea.
attribute NC_GLOBAL projects_0_end_date String 2018-08
attribute NC_GLOBAL projects_0_geolocation String Coastal Pacific, USA
attribute NC_GLOBAL projects_0_name String RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal
attribute NC_GLOBAL projects_0_project_nid String 684167
attribute NC_GLOBAL projects_0_start_date String 2015-09
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Oyster larvae vertical distribution data collected from a laboratory water column experiments to investigate the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida), July 2017.
attribute NC_GLOBAL title String Oyster larvae vertical distribution data collected from laboratory water column experiments on the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida), July 2017
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable trial   byte  
attribute trial _FillValue byte 127
attribute trial actual_range byte 1, 2
attribute trial bcodmo_name String exp_id
attribute trial description String Trial number
attribute trial long_name String Trial
attribute trial units String unitless
variable date   String  
attribute date bcodmo_name String date_local
attribute date description String Date of trial formatted as yyyy-mm-dd
attribute date long_name String Date
attribute date source_name String date
attribute date time_precision String 1970-01-01
attribute date units String unitless
variable column_name   String  
attribute column_name bcodmo_name String sample
attribute column_name description String Identifies the experimental water column treatment and replicate: AN-# = Acidic water at the top and Neutral water at the bottom ; NN-# = Neutral water at the top and Neutral water at the bottom; NA-# = Neutral water at the top and Acid water at the bottom. Acidic water was bubbled to be 400 pCO2 and neutral (ambient) water was bubbled to be 1500 pCO2
attribute column_name long_name String Column Name
attribute column_name nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute column_name units String unitless
variable count_id   byte  
attribute count_id _FillValue byte 127
attribute count_id actual_range byte 1, 2
attribute count_id bcodmo_name String replicate
attribute count_id colorBarMaximum double 100.0
attribute count_id colorBarMinimum double 0.0
attribute count_id description String Identifies the count number (1 or 2) per experimental date. The vertical positions of larvae in the columns were counted twice for each experiment; the first count at 10 minutes post-larval introduction into the column and the second count at 30 minutes post-larval introduction into the column.
attribute count_id long_name String Count Id
attribute count_id units String unitless
variable height_cm   byte  
attribute height_cm _FillValue byte 127
attribute height_cm actual_range byte 1, 20
attribute height_cm bcodmo_name String height
attribute height_cm description String The height above the bottom of the water column where larvae were counted
attribute height_cm long_name String Height Cm
attribute height_cm units String centimeters (cm)
variable middepth_cm   double  
attribute middepth_cm _FillValue double NaN
attribute middepth_cm actual_range double 0.5, 19.5
attribute middepth_cm bcodmo_name String depth
attribute middepth_cm description String The middepth of the section of the water column in which larvae were counted
attribute middepth_cm long_name String Middepth Cm
attribute middepth_cm nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute middepth_cm units String centimeters (cm)
variable larvae_count   byte  
attribute larvae_count _FillValue byte 127
attribute larvae_count actual_range byte 0, 63
attribute larvae_count bcodmo_name String count
attribute larvae_count colorBarMaximum double 100.0
attribute larvae_count colorBarMinimum double 0.0
attribute larvae_count description String The number of Olympia oyster larvae occupying that area of the water column during the count
attribute larvae_count long_name String Larvae Count
attribute larvae_count units String larvae
variable proportion_larvae   float  
attribute proportion_larvae _FillValue float NaN
attribute proportion_larvae actual_range float 0.0, 0.738
attribute proportion_larvae bcodmo_name String relative_abund
attribute proportion_larvae description String Proportion of total Olympia oyster larvae occupying that area of the water column during the count
attribute proportion_larvae long_name String Proportion Larvae
attribute proportion_larvae units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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