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Title Sum-
Institution Dataset ID
     data   graph     files  public pH measurements from laboratory water column experiments on the behavioral effects of ocean
acidification on Olympia oyster larvae (Ostrea lurida), July 2017
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Collection & Larval Rearing

We collected adult Olympia oysters (Ostrea lurida) from Fidalgo Bay in June
2017 and maintained them in a sea table with continuous flowing seawater
heated to 19-20\u00b0C at the Shannon Point Marine Center. We fed adult
oysters were fed concentrated algae once a day (Shellfish Diet, Reed
Mariculture) and utilized banjo-style filters (60-m) attached to the outflow
pipes of the sea table to catch released O. lurida larvae. We then collected
and reared larvae at 12\u00b0C in 3-L jars (2 individuals mL-1). Each jar of
larvae received a 50% water change with 0.35-m filtered sea water and were fed
Isochrysis galbana algae (50,000 cells mL-1) daily.

Experimental Design

To measure the effect of pH conditions on the vertical distribution of larvae
we established three experimental pycnocline treatments within clear
plexiglass water columns (2.5cm x 2.5cm x 30cm): (1) ambient water (400ppm) in
the top layer and acidic water in the bottom layer (1500ppm), (2) ambient
water (400ppm) in both top and bottom layers, and (3) acidic water (1500ppm)
in the top layer and ambient water (400ppm) in the bottom layer. Each water
layer was 60-mL of water and filled the column 10-cm high, so when each
experimental treatment was established it filled the column to 20-cm. We
established the experimental treatments by increasing the density of seawater
in the bottom layer by 0.003-0.005 g ml-1 using dialyzed Percoll (Mills 1984;
Podolskey & Emlet 1993). Experimental treatment water was kept at 12\u00b0C
and pre-equilibrated to the desired pCO2 level and density. We also included
blue food coloring (1 drop per 100-mL) to the dense bottom layer to more
easily visualize the density layers while establishing experimental
treatments. We set-up four replicate columns for each experimental treatment
making twelve columns total per experiment.

On the day of each experiment, we incubated the experimental treatment columns
in clear plexiglass water baths connected to a Fisher Scientific Isotemp
recirculating water bath to maintain treatment temperature at 12\u00b0C
throughout the experiment. We carefully injected 150 larvae by syringe into
the bottom 2-cm of each column with no more than 2-mL of their culture water.
Olympia oyster larvae are highly phototactic (personal observations), so we
gave the larvae 10 minutes to acclimate in darkness and then recorded their
vertical position in the water columns under infrared light. We video recorded
the larvae\u2019s vertical position in each column using an infrared uEye
camera equipped with Edmund Optics VIS-NIR Lens mounted on a motorized stand.
We later counted by eye the number of larvae per centimeter area of each
column from the videos.

Sampling and analytical procedures:

Carefully collected water with a syringe and pipet from the top 1-3cm of the
column, the bottom 1-3cm of the water column and right at the transition layer
where the top and bottom layers of water met and was visible by the blue dye
in the bottom layer of water. The water from the syringe was carefully
transferred to a clean 2 ml microcentrifuge tube and pH was measured directly
using a pH probe (Micro PerpHect Ross Ross\u00ae Combination pH electrode) and
read with a Thermo Scientific Orion Star pH meter.
attribute NC_GLOBAL awards_0_award_nid String 684166
attribute NC_GLOBAL awards_0_award_number String OCE-1538626
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538626 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Michael E. Sieracki
attribute NC_GLOBAL awards_0_program_manager_nid String 50446
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String pH measurements
Ostrea behavior lab expts, July 2017
S. Arellano, B. Olson, S. Yang (WWU)
version: 2019-01-14
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2019-01-17T14:28:24Z
attribute NC_GLOBAL date_modified String 2019-09-25T19:58:11Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.753080.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/753080 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String in-situ incubator
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to maintain treatment temperature during experiment
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 753087
attribute NC_GLOBAL instruments_0_description String A device on shipboard or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/82/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String In-situ incubator
attribute NC_GLOBAL instruments_0_instrument_nid String 494
attribute NC_GLOBAL instruments_0_supplied_name String Fisher Scientific Isotemp Circulating Water Bath
attribute NC_GLOBAL instruments_1_acronym String Benchtop pH Meter
attribute NC_GLOBAL instruments_1_dataset_instrument_description String The pH electrode was prepared before each set of measurements following instructions in the ROSS® Electrode User Guide (Thermo Fisher Scientific Inc.) and calibrated with a three-buffer calibration using Thermo ScientificTM OrionTM pH Buffer Individual Use Pouches.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 753090
attribute NC_GLOBAL instruments_1_description String An instrument consisting of an electronic voltmeter and pH-responsive electrode that gives a direct conversion of voltage differences to differences of pH at the measurement temperature. (McGraw-Hill Dictionary of Scientific and Technical Terms)
This instrument does not map to the NERC instrument vocabulary term for 'pH Sensor' which measures values in the water column. Benchtop models are typically employed for stationary lab applications.
attribute NC_GLOBAL instruments_1_instrument_name String Benchtop pH Meter
attribute NC_GLOBAL instruments_1_instrument_nid String 681
attribute NC_GLOBAL instruments_1_supplied_name String Thermo Scientific Orion Star A214 pH/ISE meter with a Micro PerpHect Ross® Combination pH electrode
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, cat, chemical, chemistry, column, column_depth_cat, column_depth_cm, column_name, data, dataset, date, depth, dmo, earth, Earth Science > Oceans > Ocean Chemistry > pH, erddap, management, name, ocean, oceanography, oceans, office, preliminary, reported, scale, science, sea, sea_water_ph_reported_on_total_scale, seawater, time, total, trial, water
attribute NC_GLOBAL keywords_vocabulary String GCMD Science Keywords
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/753080/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/753080 (external link)
attribute NC_GLOBAL param_mapping String {'753080': {'column_depth_cat': 'master - depth'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/753080/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Western Washington University
attribute NC_GLOBAL people_0_affiliation_acronym String WWU
attribute NC_GLOBAL people_0_person_name String Shawn M Arellano
attribute NC_GLOBAL people_0_person_nid String 684169
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Western Washington University
attribute NC_GLOBAL people_1_affiliation_acronym String WWU
attribute NC_GLOBAL people_1_person_name String Dr Brady M. Olson
attribute NC_GLOBAL people_1_person_nid String 51528
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Western Washington University
attribute NC_GLOBAL people_2_affiliation_acronym String WWU
attribute NC_GLOBAL people_2_person_name String Dr Sylvia Yang
attribute NC_GLOBAL people_2_person_nid String 684172
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Nancy Copley
attribute NC_GLOBAL people_3_person_nid String 50396
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String Climate stressors on larvae
attribute NC_GLOBAL projects_0_acronym String Climate stressors on larvae
attribute NC_GLOBAL projects_0_description String In the face of climate change, future distribution of animals will depend not only on whether they adjust to new conditions in their current habitat, but also on whether a species can spread to suitable locations in a changing habitat landscape. In the ocean, where most species have tiny drifting larval stages, dispersal between habitats is impacted by more than just ocean currents alone; the swimming behavior of larvae, the flow environment the larvae encounter, and the length of time the larvae spend in the water column all interact to impact the distance and direction of larval dispersal. The effects of climate change, especially ocean acidification, are already evident in shellfish species along the Pacific coast, where hatchery managers have noticed shellfish cultures with 'lazy larvae syndrome.' Under conditions of increased acidification, these 'lazy larvae' simply stop swimming; yet, larval swimming behavior is rarely incorporated into studies of ocean acidification. Furthermore, how ocean warming interacts with the effects of acidification on larvae and their swimming behaviors remains unexplored; indeed, warming could reverse 'lazy larvae syndrome.' This project uses a combination of manipulative laboratory experiments, computer modeling, and a real case study to examine whether the impacts of ocean warming and acidification on individual larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea. The project will tightly couple research with undergraduate education at Western Washington University, a primarily undergraduate university, by employing student researchers, incorporating materials into undergraduate courses, and pairing marine science student interns with art student interns to develop art projects aimed at communicating the effects of climate change to public audiences
As studies of the effects of climate stress in the marine environment progress, impacts on individual-level performance must be placed in a larger ecological context. While future climate-induced circulation changes certainly will affect larval dispersal, the effects of climate-change stressors on individual larval traits alone may have equally important impacts, significantly altering larval transport and, ultimately, species distribution. This study will experimentally examine the relationship between combined climate stressors (warming and acidification) on planktonic larval duration, morphology, and swimming behavior; create models to generate testable hypotheses about the effects of these factors on larval dispersal that can be applied across systems; and, finally, use a bio-physically coupled larval transport model to examine whether climate-impacted larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea.
attribute NC_GLOBAL projects_0_end_date String 2018-08
attribute NC_GLOBAL projects_0_geolocation String Coastal Pacific, USA
attribute NC_GLOBAL projects_0_name String RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal
attribute NC_GLOBAL projects_0_project_nid String 684167
attribute NC_GLOBAL projects_0_start_date String 2015-09
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String This dataset contains pH measurements collected from a laboratory water column experiments to investigate the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida).
attribute NC_GLOBAL title String pH measurements from laboratory water column experiments on the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida), July 2017
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable trial   byte  
attribute trial _FillValue byte 127
attribute trial actual_range byte 1, 2
attribute trial bcodmo_name String exp_id
attribute trial description String Trial number
attribute trial long_name String Trial
attribute trial units String unitless
variable date   String  
attribute date bcodmo_name String date_local
attribute date description String Date of trial formatted as yyyy-mm-dd
attribute date long_name String Date
attribute date source_name String date
attribute date time_precision String 1970-01-01
attribute date units String unitless
variable column_name   String  
attribute column_name bcodmo_name String sample
attribute column_name description String Identifies the experimental water column treatment and replicate #: AN-# = Acidic water at the top and Neutral water at the bottom ; NN-# = Neutral water at the top and Neutral water at the bottom; NA-# = Neutral water at the top and Acid water at the bottom
attribute column_name long_name String Column Name
attribute column_name nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute column_name units String unitless
variable column_depth_cat   double  
attribute column_depth_cat _FillValue double NaN
attribute column_depth_cat bcodmo_name String depth
attribute column_depth_cat description String Water column depth category where the pH sample was collected. top = 18-20 cm from bottom of water column; bottom = 1-2 cm from the bottom of the water column; transition point = middle of the water column where two treatment waters meet
attribute column_depth_cat long_name String Column Depth Cat
attribute column_depth_cat nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute column_depth_cat units String unitless
variable column_depth_cm   String  
attribute column_depth_cm bcodmo_name String depth
attribute column_depth_cm description String Water column depth in cm where the pH sample was collected.
attribute column_depth_cm long_name String Column Depth Cm
attribute column_depth_cm nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute column_depth_cm units String centimeters (cm)
variable pH   float  
attribute pH _FillValue float NaN
attribute pH actual_range float 7.43, 7.9
attribute pH bcodmo_name String pH
attribute pH colorBarMaximum double 9.0
attribute pH colorBarMinimum double 7.0
attribute pH description String pH of seawater in water column
attribute pH long_name String Sea Water Ph Reported On Total Scale
attribute pH nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/ (external link)
attribute pH units String standard pH units

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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