ERDDAP > info > bcodmo_dataset_753080

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	pH measurements from laboratory water column experiments on the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida), July 2017		I   M	background			BCO-DMO	bcodmo_dataset_753080

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Collection & Larval Rearing We collected adult Olympia oysters (Ostrea lurida) from Fidalgo Bay in June 2017 and maintained them in a sea table with continuous flowing seawater heated to 19-20\u00b0C at the Shannon Point Marine Center. We fed adult oysters were fed concentrated algae once a day (Shellfish Diet, Reed Mariculture) and utilized banjo-style filters (60-m) attached to the outflow pipes of the sea table to catch released O. lurida larvae. We then collected and reared larvae at 12\u00b0C in 3-L jars (2 individuals mL-1). Each jar of larvae received a 50% water change with 0.35-m filtered sea water and were fed Isochrysis galbana algae (50,000 cells mL-1) daily. Experimental Design To measure the effect of pH conditions on the vertical distribution of larvae we established three experimental pycnocline treatments within clear plexiglass water columns (2.5cm x 2.5cm x 30cm): (1) ambient water (400ppm) in the top layer and acidic water in the bottom layer (1500ppm), (2) ambient water (400ppm) in both top and bottom layers, and (3) acidic water (1500ppm) in the top layer and ambient water (400ppm) in the bottom layer. Each water layer was 60-mL of water and filled the column 10-cm high, so when each experimental treatment was established it filled the column to 20-cm. We established the experimental treatments by increasing the density of seawater in the bottom layer by 0.003-0.005 g ml-1 using dialyzed Percoll (Mills 1984; Podolskey & Emlet 1993). Experimental treatment water was kept at 12\u00b0C and pre-equilibrated to the desired pCO2 level and density. We also included blue food coloring (1 drop per 100-mL) to the dense bottom layer to more easily visualize the density layers while establishing experimental treatments. We set-up four replicate columns for each experimental treatment making twelve columns total per experiment. On the day of each experiment, we incubated the experimental treatment columns in clear plexiglass water baths connected to a Fisher Scientific Isotemp recirculating water bath to maintain treatment temperature at 12\u00b0C throughout the experiment. We carefully injected 150 larvae by syringe into the bottom 2-cm of each column with no more than 2-mL of their culture water. Olympia oyster larvae are highly phototactic (personal observations), so we gave the larvae 10 minutes to acclimate in darkness and then recorded their vertical position in the water columns under infrared light. We video recorded the larvae\u2019s vertical position in each column using an infrared uEye camera equipped with Edmund Optics VIS-NIR Lens mounted on a motorized stand. We later counted by eye the number of larvae per centimeter area of each column from the videos. Sampling and analytical procedures: Carefully collected water with a syringe and pipet from the top 1-3cm of the column, the bottom 1-3cm of the water column and right at the transition layer where the top and bottom layers of water met and was visible by the blue dye in the bottom layer of water. The water from the syringe was carefully transferred to a clean 2 ml microcentrifuge tube and pH was measured directly using a pH probe (Micro PerpHect Ross Ross\u00ae Combination pH electrode) and read with a Thermo Scientific Orion Star pH meter.
attribute	NC_GLOBAL	awards_0_award_nid	String	684166
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1538626
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538626
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Michael E. Sieracki
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50446
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	pH measurements Ostrea behavior lab expts, July 2017 S. Arellano, B. Olson, S. Yang (WWU) version: 2019-01-14
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2019-01-17T14:28:24Z
attribute	NC_GLOBAL	date_modified	String	2019-09-25T19:58:11Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.753080.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/753080
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	in-situ incubator
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used to maintain treatment temperature during experiment
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	753087
attribute	NC_GLOBAL	instruments_0_description	String	A device on shipboard or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/82/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	In-situ incubator
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	494
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Fisher Scientific Isotemp Circulating Water Bath
attribute	NC_GLOBAL	instruments_1_acronym	String	Benchtop pH Meter
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	The pH electrode was prepared before each set of measurements following instructions in the ROSS® Electrode User Guide (Thermo Fisher Scientific Inc.) and calibrated with a three-buffer calibration using Thermo ScientificTM OrionTM pH Buffer Individual Use Pouches.
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	753090
attribute	NC_GLOBAL	instruments_1_description	String	An instrument consisting of an electronic voltmeter and pH-responsive electrode that gives a direct conversion of voltage differences to differences of pH at the measurement temperature. (McGraw-Hill Dictionary of Scientific and Technical Terms) This instrument does not map to the NERC instrument vocabulary term for 'pH Sensor' which measures values in the water column. Benchtop models are typically employed for stationary lab applications.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Benchtop pH Meter
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	681
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Thermo Scientific Orion Star A214 pH/ISE meter with a Micro PerpHect Ross® Combination pH electrode
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, cat, chemical, chemistry, column, column_depth_cat, column_depth_cm, column_name, data, dataset, date, depth, dmo, earth, Earth Science > Oceans > Ocean Chemistry > pH, erddap, management, name, ocean, oceanography, oceans, office, preliminary, reported, scale, science, sea, sea_water_ph_reported_on_total_scale, seawater, time, total, trial, water
attribute	NC_GLOBAL	keywords_vocabulary	String	GCMD Science Keywords
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/753080/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/753080
attribute	NC_GLOBAL	param_mapping	String	{'753080': {'column_depth_cat': 'master - depth'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/753080/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Western Washington University
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	WWU
attribute	NC_GLOBAL	people_0_person_name	String	Shawn M Arellano
attribute	NC_GLOBAL	people_0_person_nid	String	684169
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Western Washington University
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WWU
attribute	NC_GLOBAL	people_1_person_name	String	Dr Brady M. Olson
attribute	NC_GLOBAL	people_1_person_nid	String	51528
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Western Washington University
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	WWU
attribute	NC_GLOBAL	people_2_person_name	String	Dr Sylvia Yang
attribute	NC_GLOBAL	people_2_person_nid	String	684172
attribute	NC_GLOBAL	people_2_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_3_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_3_person_nid	String	50396
attribute	NC_GLOBAL	people_3_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	project	String	Climate stressors on larvae
attribute	NC_GLOBAL	projects_0_acronym	String	Climate stressors on larvae
attribute	NC_GLOBAL	projects_0_description	String	In the face of climate change, future distribution of animals will depend not only on whether they adjust to new conditions in their current habitat, but also on whether a species can spread to suitable locations in a changing habitat landscape. In the ocean, where most species have tiny drifting larval stages, dispersal between habitats is impacted by more than just ocean currents alone; the swimming behavior of larvae, the flow environment the larvae encounter, and the length of time the larvae spend in the water column all interact to impact the distance and direction of larval dispersal. The effects of climate change, especially ocean acidification, are already evident in shellfish species along the Pacific coast, where hatchery managers have noticed shellfish cultures with 'lazy larvae syndrome.' Under conditions of increased acidification, these 'lazy larvae' simply stop swimming; yet, larval swimming behavior is rarely incorporated into studies of ocean acidification. Furthermore, how ocean warming interacts with the effects of acidification on larvae and their swimming behaviors remains unexplored; indeed, warming could reverse 'lazy larvae syndrome.' This project uses a combination of manipulative laboratory experiments, computer modeling, and a real case study to examine whether the impacts of ocean warming and acidification on individual larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea. The project will tightly couple research with undergraduate education at Western Washington University, a primarily undergraduate university, by employing student researchers, incorporating materials into undergraduate courses, and pairing marine science student interns with art student interns to develop art projects aimed at communicating the effects of climate change to public audiences As studies of the effects of climate stress in the marine environment progress, impacts on individual-level performance must be placed in a larger ecological context. While future climate-induced circulation changes certainly will affect larval dispersal, the effects of climate-change stressors on individual larval traits alone may have equally important impacts, significantly altering larval transport and, ultimately, species distribution. This study will experimentally examine the relationship between combined climate stressors (warming and acidification) on planktonic larval duration, morphology, and swimming behavior; create models to generate testable hypotheses about the effects of these factors on larval dispersal that can be applied across systems; and, finally, use a bio-physically coupled larval transport model to examine whether climate-impacted larvae may affect the distribution and restoration of populations of native oysters in the Salish Sea.
attribute	NC_GLOBAL	projects_0_end_date	String	2018-08
attribute	NC_GLOBAL	projects_0_geolocation	String	Coastal Pacific, USA
attribute	NC_GLOBAL	projects_0_name	String	RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal
attribute	NC_GLOBAL	projects_0_project_nid	String	684167
attribute	NC_GLOBAL	projects_0_start_date	String	2015-09
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	This dataset contains pH measurements collected from a laboratory water column experiments to investigate the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida).
attribute	NC_GLOBAL	title	String	pH measurements from laboratory water column experiments on the behavioral effects of ocean acidification on Olympia oyster larvae (Ostrea lurida), July 2017
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	trial		byte
attribute	trial	_FillValue	byte	127
attribute	trial	actual_range	byte	1, 2
attribute	trial	bcodmo_name	String	exp_id
attribute	trial	description	String	Trial number
attribute	trial	long_name	String	Trial
attribute	trial	units	String	unitless
variable	date		String
attribute	date	bcodmo_name	String	date_local
attribute	date	description	String	Date of trial formatted as yyyy-mm-dd
attribute	date	long_name	String	Date
attribute	date	source_name	String	date
attribute	date	time_precision	String	1970-01-01
attribute	date	units	String	unitless
variable	column_name		String
attribute	column_name	bcodmo_name	String	sample
attribute	column_name	description	String	Identifies the experimental water column treatment and replicate #: AN-# = Acidic water at the top and Neutral water at the bottom ; NN-# = Neutral water at the top and Neutral water at the bottom; NA-# = Neutral water at the top and Acid water at the bottom
attribute	column_name	long_name	String	Column Name
attribute	column_name	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	column_name	units	String	unitless
variable	column_depth_cat		double
attribute	column_depth_cat	_FillValue	double	NaN
attribute	column_depth_cat	bcodmo_name	String	depth
attribute	column_depth_cat	description	String	Water column depth category where the pH sample was collected. top = 18-20 cm from bottom of water column; bottom = 1-2 cm from the bottom of the water column; transition point = middle of the water column where two treatment waters meet
attribute	column_depth_cat	long_name	String	Column Depth Cat
attribute	column_depth_cat	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/DEPH/
attribute	column_depth_cat	units	String	unitless
variable	column_depth_cm		String
attribute	column_depth_cm	bcodmo_name	String	depth
attribute	column_depth_cm	description	String	Water column depth in cm where the pH sample was collected.
attribute	column_depth_cm	long_name	String	Column Depth Cm
attribute	column_depth_cm	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/DEPH/
attribute	column_depth_cm	units	String	centimeters (cm)
variable	pH		float
attribute	pH	_FillValue	float	NaN
attribute	pH	actual_range	float	7.43, 7.9
attribute	pH	bcodmo_name	String	pH
attribute	pH	colorBarMaximum	double	9.0
attribute	pH	colorBarMinimum	double	7.0
attribute	pH	description	String	pH of seawater in water column
attribute	pH	long_name	String	Sea Water Ph Reported On Total Scale
attribute	pH	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PHXXZZXX/
attribute	pH	units	String	standard pH units

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.