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   set  data   graph     files  public Chlorophyll measurements from HHQ experiments conducted during the MesoHux mesocosm
experiment, May 2017, Bergen, Norway
   ?     I   M   background (external link) RSS BCO-DMO bcodmo_dataset_753388

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Water samples for chlorophyll extraction were collected either from the
mesocosms via a 5 L Niskin or subsampled from experimental bottles.
Chlorophyll samples were filtered in triplicate through a 25mm Glass Fiber
Filter (GFF), and immediately extracted in 6 mL of ethanol for 12-18 hours in
the dark at room temperature. After extraction, filters were removed from the
sample, and the fluorescence of the sample was read on a Turner AU10. The
sample was then acidified with 1 drop of 10% HCL and re-read on the same
instrument. The fluorometer was calibrated prior to using with a chlorophyll
standard purchased from Sigma.\u00a0

Mesocosm treatment for all HHQ experiments was as follows:
Redfield: N:P added in a 16:1 ratio during the first 3 days of the
experiment, no shading

HHQ treatments here are as follows:
High HHQ - 100 ng mL-1 (410 uM) added to triplicate 5L bottles.
DMSO control - equivalent (v:v) DMSO added to triplicate 5L bottles.

\u00a0All bottles were incubated for 24h in a flow-through tank, that was
shaded to mimic in situ conditions. Chlorophyll samples were taken at T0 and
T24 for all experiments.

Data were processed in Excel with statistics run in Excel, R, or Matlab.
attribute NC_GLOBAL awards_0_award_nid String 709952
attribute NC_GLOBAL awards_0_award_number String OCE-1657898
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1657898 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String Dr David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String HHQ Chlorophyll
from MesoHux mesocosm experiment, May 2017, Bergen, Norway
PI: E. Harvey (SkIO)
version: 2019-01-23
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.2d 13 Jun 2019
attribute NC_GLOBAL date_created String 2019-01-23T15:43:33Z
attribute NC_GLOBAL date_modified String 2019-03-14T19:44:45Z
attribute NC_GLOBAL defaultDataQuery String &time
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.753388.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/753388 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Niskin bottle
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Used to collect water samples.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 753396
attribute NC_GLOBAL instruments_0_description String A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24 or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Niskin bottle
attribute NC_GLOBAL instruments_0_instrument_nid String 413
attribute NC_GLOBAL instruments_0_supplied_name String 5 L Niskin
attribute NC_GLOBAL instruments_1_acronym String Fluorometer
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 753399
attribute NC_GLOBAL instruments_1_description String A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/113/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Fluorometer
attribute NC_GLOBAL instruments_1_instrument_nid String 484
attribute NC_GLOBAL instruments_1_supplied_name String Turner AU10 fluorometer
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, blank, chemical, chl, chlorophyll, data, dataset, date, dilution, Dilution_Factor, dmo, erddap, experiment, Experiment_num, extract, Extract_Volume_mL, F_a, F_a_blank, F_o, F_o_blank, factor, filtered, management, num, oceanography, office, phaeo, preliminary, replication, sample, time, time2, total, Total_Chl_no_phaeo, Total_chl_with_phaeo, volume, Volume_Filtered_mL, with
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/753388 (external link)
attribute NC_GLOBAL param_mapping String {'753388': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/753388/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Skidaway Institute of Oceanography
attribute NC_GLOBAL people_0_affiliation_acronym String SkIO
attribute NC_GLOBAL people_0_person_name String Dr Elizabeth Harvey
attribute NC_GLOBAL people_0_person_nid String 645518
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of Rhode Island
attribute NC_GLOBAL people_1_affiliation_acronym String URI
attribute NC_GLOBAL people_1_person_name String Dr David Rowley
attribute NC_GLOBAL people_1_person_nid String 709954
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Haverford College
attribute NC_GLOBAL people_2_affiliation_acronym String Haveford
attribute NC_GLOBAL people_2_person_name String Dr Kristen E. Whalen
attribute NC_GLOBAL people_2_person_nid String 709960
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Nancy Copley
attribute NC_GLOBAL people_3_person_nid String 50396
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String Collaborative Research: Building a framework for the role of bacterial-derived chemical signals in mediating phytoplankton population dynamics
attribute NC_GLOBAL projects_0_acronym String HHQSignals
attribute NC_GLOBAL projects_0_description String NSF Abstract:
Bacteria and phytoplankton play a central role in the modification and flow of materials and nutrients through the marine environment. While it has been established that interactions between these two domains are complex, the mechanisms that underpin these interactions remain largely unknown. There is increasing recognition, however, that dissolved chemical cues govern these microbial interactions. This project focuses on establishing a mechanistic framework for how bacterially derived signaling molecules influence interactions between phytoplankton and bacteria. The quorum-sensing (QS) molecule, 2-heptyl-4-quinolone (HHQ) will be used as a model compound for these investigations. Previously published work suggests that exposure to very low levels of HHQ results in phytoplankton mortality. Gaining a mechanistic understanding of these ecologically important interactions will help to inform mathematical models for the accurate prediction of the cycling of material through the marine microbial loop. This work initiates a new, hybrid workshop-internship undergraduate research program in chemical ecology, with a focus
Bacteria and phytoplankton play a central role in the modification and flow of materials and nutrients through the marine environment. While it has been established that interactions between these two domains are complex, the mechanisms that underpin these interactions remain largely unknown. There is increasing recognition, however, that dissolved chemical cues govern these microbial interactions. This project focuses on establishing a mechanistic framework for how bacterially derived signaling molecules influence interactions between phytoplankton and bacteria. The quorum-sensing (QS) molecule, 2-heptyl-4-quinolone (HHQ) will be used as a model compound for these investigations. Previously published work suggests that exposure to very low levels of HHQ results in phytoplankton mortality. Gaining a mechanistic understanding of these ecologically important interactions will help to inform mathematical models for the accurate prediction of the cycling of material through the marine microbial loop. This work initiates a new, hybrid workshop-internship undergraduate research program in chemical ecology, with a focus into bacteria-phytoplankton interactions. Undergraduate students participate in an intense summer learning experience where research and field-based exercises are supplemented with short-lecture based modules. Students return to their home institutions and work closely with the PIs to conduct interdisciplinary research relating to the aims and scope of the summer research. This research also provides training and career development to two graduate students and a postdoctoral scientist.
Interactions between phytoplankton and bacteria play a central role in mediating biogeochemical cycling and microbial trophic structure in the ocean. The intricate relationships between these two domains of life are mediated via excreted molecules that facilitate communication and determine competitive outcomes. Despite their predicted importance, identifying these released compounds has remained a challenge. The PIs recently identified a bacterial QS molecule, HHQ, produced by globally distributed marine gamma-proteobacteria, which induces phytoplankton mortality. The PIs therefore hypothesize that bacteria QS signals are critical drivers of phytoplankton population dynamics and, ultimately, biogeochemical fluxes. This project investigates the timing and magnitude of HHQ production, and the physiological and transcriptomic responses of susceptible phytoplankton species to HHQ exposure, and quantifies the influence of HHQ on natural algal and bacterial assemblages. The work connects laboratory and field-based experiments to understand the governance of chemical signaling on marine microbial interactions, and has the potential to yield broadly applicable insights into how microbial interactions influence biogeochemical fluxes in the marine environment.
attribute NC_GLOBAL projects_0_end_date String 2020-03
attribute NC_GLOBAL projects_0_geolocation String Bergen, Norway
attribute NC_GLOBAL projects_0_name String Collaborative Research: Building a framework for the role of bacterial-derived chemical signals in mediating phytoplankton population dynamics
attribute NC_GLOBAL projects_0_project_nid String 709948
attribute NC_GLOBAL projects_0_start_date String 2017-04
attribute NC_GLOBAL publisher_name String Nancy Copley
attribute NC_GLOBAL publisher_role String BCO-DMO Data Manager(s)
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v29
attribute NC_GLOBAL subsetVariables String Extract_Volume_mL, Dilution_Factor
attribute NC_GLOBAL summary String This dataset includes chlorophyll measurements from HHQ experiments conducted during the MesoHux mesocosm experiment, May 2017, Bergen, Norway. Microbial mesocosms were spiked with 2-heptyl-4-quinolone (HHQ).
attribute NC_GLOBAL title String Chlorophyll measurements from HHQ experiments conducted during the MesoHux mesocosm experiment, May 2017, Bergen, Norway
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5-beta
variable Date   String  
attribute Date description String sampling date formatted as Mon dd yyyy
attribute Date ioos_category String Time
attribute Date long_name String Date
attribute Date units String unitless
variable Sample   String  
attribute Sample description String sample identifier
attribute Sample ioos_category String Unknown
attribute Sample long_name String Sample
attribute Sample units String unitless
variable Experiment_num   byte  
attribute Experiment_num _FillValue byte 127
attribute Experiment_num actual_range byte 1, 8
attribute Experiment_num description String experiment number
attribute Experiment_num ioos_category String Unknown
attribute Experiment_num long_name String Experiment Num
attribute Experiment_num units String unitless
variable time2   byte  
attribute time2 _FillValue byte 127
attribute time2 actual_range byte 0, 24
attribute time2 description String time since start of experiment
attribute time2 ioos_category String Time
attribute time2 long_name String Time
attribute time2 units String hours
variable Replication   byte  
attribute Replication _FillValue byte 127
attribute Replication actual_range byte 1, 3
attribute Replication description String replicate number
attribute Replication ioos_category String Unknown
attribute Replication long_name String Replication
attribute Replication units String unitless
variable Volume_Filtered_mL   short  
attribute Volume_Filtered_mL _FillValue short 32767
attribute Volume_Filtered_mL actual_range short 100, 155
attribute Volume_Filtered_mL description String volume filtered
attribute Volume_Filtered_mL ioos_category String Unknown
attribute Volume_Filtered_mL long_name String Volume Filtered M L
attribute Volume_Filtered_mL units String milliliters (mL)
variable Extract_Volume_mL   byte  
attribute Extract_Volume_mL _FillValue byte 127
attribute Extract_Volume_mL actual_range byte 6, 6
attribute Extract_Volume_mL description String volume extracted
attribute Extract_Volume_mL ioos_category String Unknown
attribute Extract_Volume_mL long_name String Extract Volume M L
attribute Extract_Volume_mL units String milliliters (mL)
variable Dilution_Factor   byte  
attribute Dilution_Factor _FillValue byte 127
attribute Dilution_Factor actual_range byte 1, 1
attribute Dilution_Factor description String dilution factor
attribute Dilution_Factor ioos_category String Unknown
attribute Dilution_Factor long_name String Dilution Factor
attribute Dilution_Factor units String unitless
variable F_o   float  
attribute F_o _FillValue float NaN
attribute F_o actual_range float 11.4, 176.0
attribute F_o description String initial fluorescence reading
attribute F_o ioos_category String Unknown
attribute F_o long_name String F O
attribute F_o units String Relative Fluorescence Units (RFU)
variable F_o_blank   float  
attribute F_o_blank _FillValue float NaN
attribute F_o_blank actual_range float 11.4, 176.0
attribute F_o_blank description String initial fluorescence of control blank
attribute F_o_blank ioos_category String Unknown
attribute F_o_blank long_name String F O Blank
attribute F_o_blank units String Relative Fluorescence Units (RFU)
variable F_a   float  
attribute F_a _FillValue float NaN
attribute F_a actual_range float 6.49, 96.9
attribute F_a description String fluorescence after acidification
attribute F_a ioos_category String Unknown
attribute F_a long_name String F A
attribute F_a units String Relative Fluorescence Units (RFU)
variable F_a_blank   float  
attribute F_a_blank _FillValue float NaN
attribute F_a_blank actual_range float 6.49, 96.9
attribute F_a_blank description String fluorescence of control blank after acidification
attribute F_a_blank ioos_category String Unknown
attribute F_a_blank long_name String F A Blank
attribute F_a_blank units String Relative Fluorescence Units (RFU)
variable Total_chl_with_phaeo   float  
attribute Total_chl_with_phaeo _FillValue float NaN
attribute Total_chl_with_phaeo actual_range float 0.5, 6.77
attribute Total_chl_with_phaeo description String total chlorophyll including phaeophytin
attribute Total_chl_with_phaeo ioos_category String Unknown
attribute Total_chl_with_phaeo long_name String Total Chl With Phaeo
attribute Total_chl_with_phaeo units String micrograms chlorophyll/Liter (ug/L)
variable Total_Chl_no_phaeo   float  
attribute Total_Chl_no_phaeo _FillValue float NaN
attribute Total_Chl_no_phaeo actual_range float 0.42, 6.42
attribute Total_Chl_no_phaeo description String total chlorophyll NOT including phaeophytin
attribute Total_Chl_no_phaeo ioos_category String Unknown
attribute Total_Chl_no_phaeo long_name String Total Chl No Phaeo
attribute Total_Chl_no_phaeo units String micrograms chlorophyll/Liter (ug/L)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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