ERDDAP > info > bcodmo_dataset_753388

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
	set	data	graph		files	public	[HHQ Chlorophyll] - Chlorophyll measurements from HHQ experiments conducted during the MesoHux mesocosm experiment, May 2017, Bergen, Norway (Collaborative Research: Building a framework for the role of bacterial-derived chemical signals in mediating phytoplankton population dynamics)		I   M	background			BCO-DMO	bcodmo_dataset_753388

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Water samples for chlorophyll extraction were collected either from the mesocosms via a 5 L Niskin or subsampled from experimental bottles. Chlorophyll samples were filtered in triplicate through a 25mm Glass Fiber Filter (GFF), and immediately extracted in 6 mL of ethanol for 12-18 hours in the dark at room temperature. After extraction, filters were removed from the sample, and the fluorescence of the sample was read on a Turner AU10. The sample was then acidified with 1 drop of 10% HCL and re-read on the same instrument. The fluorometer was calibrated prior to using with a chlorophyll standard purchased from Sigma.\u00a0 Mesocosm treatment for all HHQ experiments was as follows: Redfield: N:P added in a 16:1 ratio during the first 3 days of the experiment, no shading HHQ treatments here are as follows: High HHQ - 100 ng mL-1 (410 uM) added to triplicate 5L bottles. DMSO control - equivalent (v:v) DMSO added to triplicate 5L bottles. \u00a0All bottles were incubated for 24h in a flow-through tank, that was shaded to mimic in situ conditions. Chlorophyll samples were taken at T0 and T24 for all experiments. Data were processed in Excel with statistics run in Excel, R, or Matlab.
attribute	NC_GLOBAL	awards_0_award_nid	String	709952
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1657898
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1657898
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	HHQ Chlorophyll from MesoHux mesocosm experiment, May 2017, Bergen, Norway PI: E. Harvey (SkIO) version: 2019-01-23
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2019-01-23T15:43:33Z
attribute	NC_GLOBAL	date_modified	String	2019-03-14T19:44:45Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.753388.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/753388
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Niskin bottle
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Used to collect water samples.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	753396
attribute	NC_GLOBAL	instruments_0_description	String	A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24 or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Niskin bottle
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	413
attribute	NC_GLOBAL	instruments_0_supplied_name	String	5 L Niskin
attribute	NC_GLOBAL	instruments_1_acronym	String	Fluorometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	753399
attribute	NC_GLOBAL	instruments_1_description	String	A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/113/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Fluorometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	484
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Turner AU10 fluorometer
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, blank, chemical, chl, chlorophyll, data, dataset, date, dilution, Dilution_Factor, dmo, erddap, experiment, Experiment_num, extract, Extract_Volume_mL, F_a, F_a_blank, F_o, F_o_blank, factor, filtered, management, num, oceanography, office, phaeo, preliminary, replication, sample, time, time2, total, Total_Chl_no_phaeo, Total_chl_with_phaeo, volume, Volume_Filtered_mL, with
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/753388/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/753388
attribute	NC_GLOBAL	param_mapping	String	{'753388': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/753388/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Skidaway Institute of Oceanography
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	SkIO
attribute	NC_GLOBAL	people_0_person_name	String	Elizabeth Harvey
attribute	NC_GLOBAL	people_0_person_nid	String	645518
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	University of Rhode Island
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	URI
attribute	NC_GLOBAL	people_1_person_name	String	Dr David Rowley
attribute	NC_GLOBAL	people_1_person_nid	String	709954
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Haverford College
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	Haveford
attribute	NC_GLOBAL	people_2_person_name	String	Kristen E. Whalen
attribute	NC_GLOBAL	people_2_person_nid	String	709960
attribute	NC_GLOBAL	people_2_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_3_person_name	String	Nancy Copley
attribute	NC_GLOBAL	people_3_person_nid	String	50396
attribute	NC_GLOBAL	people_3_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	project	String	HHQSignals
attribute	NC_GLOBAL	projects_0_acronym	String	HHQSignals
attribute	NC_GLOBAL	projects_0_description	String	NSF Abstract: Bacteria and phytoplankton play a central role in the modification and flow of materials and nutrients through the marine environment. While it has been established that interactions between these two domains are complex, the mechanisms that underpin these interactions remain largely unknown. There is increasing recognition, however, that dissolved chemical cues govern these microbial interactions. This project focuses on establishing a mechanistic framework for how bacterially derived signaling molecules influence interactions between phytoplankton and bacteria. The quorum-sensing (QS) molecule, 2-heptyl-4-quinolone (HHQ) will be used as a model compound for these investigations. Previously published work suggests that exposure to very low levels of HHQ results in phytoplankton mortality. Gaining a mechanistic understanding of these ecologically important interactions will help to inform mathematical models for the accurate prediction of the cycling of material through the marine microbial loop. This work initiates a new, hybrid workshop-internship undergraduate research program in chemical ecology, with a focus Bacteria and phytoplankton play a central role in the modification and flow of materials and nutrients through the marine environment. While it has been established that interactions between these two domains are complex, the mechanisms that underpin these interactions remain largely unknown. There is increasing recognition, however, that dissolved chemical cues govern these microbial interactions. This project focuses on establishing a mechanistic framework for how bacterially derived signaling molecules influence interactions between phytoplankton and bacteria. The quorum-sensing (QS) molecule, 2-heptyl-4-quinolone (HHQ) will be used as a model compound for these investigations. Previously published work suggests that exposure to very low levels of HHQ results in phytoplankton mortality. Gaining a mechanistic understanding of these ecologically important interactions will help to inform mathematical models for the accurate prediction of the cycling of material through the marine microbial loop. This work initiates a new, hybrid workshop-internship undergraduate research program in chemical ecology, with a focus into bacteria-phytoplankton interactions. Undergraduate students participate in an intense summer learning experience where research and field-based exercises are supplemented with short-lecture based modules. Students return to their home institutions and work closely with the PIs to conduct interdisciplinary research relating to the aims and scope of the summer research. This research also provides training and career development to two graduate students and a postdoctoral scientist. Interactions between phytoplankton and bacteria play a central role in mediating biogeochemical cycling and microbial trophic structure in the ocean. The intricate relationships between these two domains of life are mediated via excreted molecules that facilitate communication and determine competitive outcomes. Despite their predicted importance, identifying these released compounds has remained a challenge. The PIs recently identified a bacterial QS molecule, HHQ, produced by globally distributed marine gamma-proteobacteria, which induces phytoplankton mortality. The PIs therefore hypothesize that bacteria QS signals are critical drivers of phytoplankton population dynamics and, ultimately, biogeochemical fluxes. This project investigates the timing and magnitude of HHQ production, and the physiological and transcriptomic responses of susceptible phytoplankton species to HHQ exposure, and quantifies the influence of HHQ on natural algal and bacterial assemblages. The work connects laboratory and field-based experiments to understand the governance of chemical signaling on marine microbial interactions, and has the potential to yield broadly applicable insights into how microbial interactions influence biogeochemical fluxes in the marine environment.
attribute	NC_GLOBAL	projects_0_end_date	String	2020-03
attribute	NC_GLOBAL	projects_0_geolocation	String	Bergen, Norway
attribute	NC_GLOBAL	projects_0_name	String	Collaborative Research: Building a framework for the role of bacterial-derived chemical signals in mediating phytoplankton population dynamics
attribute	NC_GLOBAL	projects_0_project_nid	String	709948
attribute	NC_GLOBAL	projects_0_start_date	String	2017-04
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	subsetVariables	String	Extract_Volume_mL,Dilution_Factor
attribute	NC_GLOBAL	summary	String	This dataset includes chlorophyll measurements from HHQ experiments conducted during the MesoHux mesocosm experiment, May 2017, Bergen, Norway. Microbial mesocosms were spiked with 2-heptyl-4-quinolone (HHQ).
attribute	NC_GLOBAL	title	String	[HHQ Chlorophyll] - Chlorophyll measurements from HHQ experiments conducted during the MesoHux mesocosm experiment, May 2017, Bergen, Norway (Collaborative Research: Building a framework for the role of bacterial-derived chemical signals in mediating phytoplankton population dynamics)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	Date		String
attribute	Date	bcodmo_name	String	date
attribute	Date	description	String	sampling date formatted as Mon dd yyyy
attribute	Date	long_name	String	Date
attribute	Date	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	Date	units	String	unitless
variable	Sample		String
attribute	Sample	bcodmo_name	String	sample
attribute	Sample	description	String	sample identifier
attribute	Sample	long_name	String	Sample
attribute	Sample	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/ACYC/
attribute	Sample	units	String	unitless
variable	Experiment_num		byte
attribute	Experiment_num	_FillValue	byte	127
attribute	Experiment_num	actual_range	byte	1, 8
attribute	Experiment_num	bcodmo_name	String	exp_id
attribute	Experiment_num	description	String	experiment number
attribute	Experiment_num	long_name	String	Experiment Num
attribute	Experiment_num	units	String	unitless
variable	time2		byte
attribute	time2	_FillValue	byte	127
attribute	time2	actual_range	byte	0, 24
attribute	time2	bcodmo_name	String	incubation time
attribute	time2	description	String	time since start of experiment
attribute	time2	long_name	String	Time
attribute	time2	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/AZDRZZ01/
attribute	time2	units	String	hours
variable	Replication		byte
attribute	Replication	_FillValue	byte	127
attribute	Replication	actual_range	byte	1, 3
attribute	Replication	bcodmo_name	String	replicate
attribute	Replication	description	String	replicate number
attribute	Replication	long_name	String	Replication
attribute	Replication	units	String	unitless
variable	Volume_Filtered_mL		short
attribute	Volume_Filtered_mL	_FillValue	short	32767
attribute	Volume_Filtered_mL	actual_range	short	100, 155
attribute	Volume_Filtered_mL	bcodmo_name	String	fluor_vol_filt
attribute	Volume_Filtered_mL	description	String	volume filtered
attribute	Volume_Filtered_mL	long_name	String	Volume Filtered M L
attribute	Volume_Filtered_mL	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/VOLFMCXX/
attribute	Volume_Filtered_mL	units	String	milliliters (mL)
variable	Extract_Volume_mL		byte
attribute	Extract_Volume_mL	_FillValue	byte	127
attribute	Extract_Volume_mL	actual_range	byte	6, 6
attribute	Extract_Volume_mL	bcodmo_name	String	volume
attribute	Extract_Volume_mL	description	String	volume extracted
attribute	Extract_Volume_mL	long_name	String	Extract Volume M L
attribute	Extract_Volume_mL	units	String	milliliters (mL)
variable	Dilution_Factor		byte
attribute	Dilution_Factor	_FillValue	byte	127
attribute	Dilution_Factor	actual_range	byte	1, 1
attribute	Dilution_Factor	bcodmo_name	String	dilution
attribute	Dilution_Factor	description	String	dilution factor
attribute	Dilution_Factor	long_name	String	Dilution Factor
attribute	Dilution_Factor	units	String	unitless
variable	F_o		float
attribute	F_o	_FillValue	float	NaN
attribute	F_o	actual_range	float	11.4, 176.0
attribute	F_o	bcodmo_name	String	fluorescence
attribute	F_o	description	String	initial fluorescence reading
attribute	F_o	long_name	String	F O
attribute	F_o	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/
attribute	F_o	units	String	Relative Fluorescence Units (RFU)
variable	F_o_blank		float
attribute	F_o_blank	_FillValue	float	NaN
attribute	F_o_blank	actual_range	float	11.4, 176.0
attribute	F_o_blank	bcodmo_name	String	fluorescence
attribute	F_o_blank	description	String	initial fluorescence of control blank
attribute	F_o_blank	long_name	String	F O Blank
attribute	F_o_blank	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/
attribute	F_o_blank	units	String	Relative Fluorescence Units (RFU)
variable	F_a		float
attribute	F_a	_FillValue	float	NaN
attribute	F_a	actual_range	float	6.49, 96.9
attribute	F_a	bcodmo_name	String	fluorescence
attribute	F_a	description	String	fluorescence after acidification
attribute	F_a	long_name	String	F A
attribute	F_a	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/
attribute	F_a	units	String	Relative Fluorescence Units (RFU)
variable	F_a_blank		float
attribute	F_a_blank	_FillValue	float	NaN
attribute	F_a_blank	actual_range	float	6.49, 96.9
attribute	F_a_blank	bcodmo_name	String	fluorescence
attribute	F_a_blank	description	String	fluorescence of control blank after acidification
attribute	F_a_blank	long_name	String	F A Blank
attribute	F_a_blank	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/
attribute	F_a_blank	units	String	Relative Fluorescence Units (RFU)
variable	Total_chl_with_phaeo		float
attribute	Total_chl_with_phaeo	_FillValue	float	NaN
attribute	Total_chl_with_phaeo	actual_range	float	0.5, 6.77
attribute	Total_chl_with_phaeo	bcodmo_name	String	chl_tot
attribute	Total_chl_with_phaeo	description	String	total chlorophyll including phaeophytin
attribute	Total_chl_with_phaeo	long_name	String	Total Chl With Phaeo
attribute	Total_chl_with_phaeo	units	String	micrograms chlorophyll/Liter (ug/L)
variable	Total_Chl_no_phaeo		float
attribute	Total_Chl_no_phaeo	_FillValue	float	NaN
attribute	Total_Chl_no_phaeo	actual_range	float	0.42, 6.42
attribute	Total_Chl_no_phaeo	bcodmo_name	String	unknown
attribute	Total_Chl_no_phaeo	description	String	total chlorophyll NOT including phaeophytin
attribute	Total_Chl_no_phaeo	long_name	String	Total Chl No Phaeo
attribute	Total_Chl_no_phaeo	units	String	micrograms chlorophyll/Liter (ug/L)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.